Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,9010TA30.3% 38.0 / 11.9 34Y44F (TAT→TTT) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (13/10);  new base A (0/10);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 2.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTT  >  NC_000913/4336658‑4337137
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            
aaTACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGc                                                                                                                                                                                                                                       <  1:30311/251‑1 (MQ=255)
               ttCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGt                                                                                                                                                                                                                           >  1:164660/1‑248 (MQ=255)
                   gAATCGGGAAGACGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGGAAGCGTAAGAACCACCAGGACTTTGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAAAGCCCCCGTTAAC‑CGATAATCGTCACCAACCATCCCCAAATGGCAATCCCGCCAggaacagcc                                                                                                                                                                                                                    >  2:324216/1‑243 (MQ=255)
                       cGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGt                                                                                                                                                                                                                >  1:192396/1‑251 (MQ=255)
                           aagaagTAACTTAAATATCCTCCGCCAATGCCCACCATGGCGATATTGCCGACCCAGCAGGCCAGCCAGTAGAGGACGTTGTTTTGATAACCGAGAACCGGGCCAAAGCAGCGGGGGGCGTAAGGTTAAGACCCACCAGGCTTTGGGTGGAGAAACGACTTTTTGGCTTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCCCCAACCACCCATAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGc                                                                                                                                                                                                            <  1:8543/251‑1 (MQ=255)
                                         atatCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGaaaa                                                                                                                                                                                               >  1:154509/1‑250 (MQ=255)
                                          aaccctccccaaatgccccccagggCGTTATTGCCGACCCAGCAGCCCAGCAAGTGGAGGAGGTTGTTTTGATAACGGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGACCCACCAGGTCTTGGGTGGGGGAACGACTTTTTGGCGTATC‑CCAGCGAGAGCCCCAGTGCCC‑CGATAATCGTCACCAACCACCCAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAac                                                                                                                                                                                             <  1:256657/228‑1 (MQ=255)
                                              ctacgcacaTGACCACCATGGCGATATTGCCGATCCAGAAGGCCCGCCCGTAGACGACGTTGGTTTGATACCCGAGAAACGGGCCAAAGCAGCGGCGGGCGTACACGTACGAACCACCCCGACTTGGGGCGCGGCAAGAAATTTTGGCGTATA‑CAATCGAGACCGCCAGCGCAC‑CGCTAAGCCACACCAACCATACATAAATAGCAATACCGCCCGCACAGGCCAGGTTTTAAGGTAACAGaaaaaaacat                                                                                                                                                                                         >  2:365168/9‑246 (MQ=255)
                                                  gCCAATGACGACCATGGAGCTATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGAAGCGGCGGGGGGAAGGGAAAAAACCACCAGGACGTGGGTCGAGGAAAGCCATTTTGGCGCAGA‑CCATCGAGGACCCCAGTCCACTGGGGAGTC‑TCACCAAGCAACCATAAATGGAAATTCCGCCCACAGAGGCCAGGGTTTCAGGTAATAGAAAAACACGTGAcc                                                                                                                                                                                     >  2:484560/1‑251 (MQ=255)
                                                   caaatgccccccaggccaatatTGCCGACCCAGCAGGCCGGCCAGAAGGGGACGTTGGTTTGAAACCCGAGAAACGGGCCAAAGCAGCGGGGGGCGTAAGCGAAAGAACCCCCAGTACTGGGGGCGAGGAACGACATTTGGGCGTATC‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCCCCACCCACCCATAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAccc                                                                                                                                                                                    <  1:107290/233‑1 (MQ=255)
                                                         cccaccaGGGCGATATTCCCGATCCAGCGGGCCAGCCAGTAGAGGACGTTGTTTTAATAACCGAGAACCGGCCCAAAGCAGCGGGGGGCGTAGGCGTAAGAACCCCCAGGACTGGGGTCGAGGAACGACTTTTGGGCGTATC‑CCATCGAGAGCCCCATTGCCC‑CAATAAGCGTCACCACCCATCAAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataat                                                                                                                                                                              <  1:175334/250‑1 (MQ=255)
                                                                                         cagccagTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAtt                                                                                                                                               >  1:333420/1‑250 (MQ=255)
                                                                                            ccagTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACCACATTTTGGCGTATA‑CCATCGAGAGCCCCCGTGCAC‑CGATAATCGTCACCCACCATCCCTAAATGGCCATCCCCCCCGTAGAGGCCCGGCATGCCGGTAACAGAAAAACACCTTACCCCATAATATTCCCCGACACCATCCGGGTgtcgaggcttaac                                                                                                                                            >  2:228435/1‑238 (MQ=255)
                                                                                                           ggTTTGATAACCGAGAAACGGGCCAAAGCGGCGGGGGTCGTAAGCGAAAAACCCACCAGTACTTGGGTCGAGGAACGACTTTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAACCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagca                                                                                                                             <  1:505146/250‑1 (MQ=255)
                                                                                                           ggTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAAAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGCAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcat                                                                                                                            >  1:401122/1‑251 (MQ=255)
                                                                                                               tGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                        >  1:177305/1‑251 (MQ=255)
                                                                                                                        agagaggggCCAAAGCAGCGGCGGGGGGAAGCGTAAGAACCCCCAGGAGTGGGTTGGAGGACCGA‑TCTTAGGGGTTTAACCATCGAGAGCCCCGGGGCAC‑AGATAATCGTCACCCACAAACCAAAAAGGGCCACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGCGCATCAGCATCCGAAGa                                                                                                               <  2:223636/245‑1 (MQ=255)
                                                                                                                         gaagcggcccAAAGCGGGGGCGGGCGAAAGCAAAAAACCCCCCAGGACTTGGGTCGAGGAACTACATTTTGGCGTATC‑CCATCGAGAGCCCCAGTGCAC‑AGAAACTCGCCACCAACCACCCAAAAAGGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGAc                                                                                                              <  2:333420/243‑1 (MQ=255)
                                                                                                                                  cAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                                      >  1:210727/1‑250 (MQ=255)
                                                                                                                                    aaGCAGGGGGGGGCGTAAGCGTAAGACCCACCAGGACTGGGGTCGAGGAACGACATTTGGGCGTATC‑CCACCGAGAGCCCCAGGGCAC‑CGATAATCGTCACCAACCACCAATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACt                                                                                                   <  1:389121/251‑1 (MQ=255)
                                                                                                                                         ggggcgggcaTAAGCGAAAAAACCACCGGGGTTTGGGTCGAGGACCGACATTTTGGGGTATC‑CCACCGAGGGCCCCAGTGAAC‑CGAAAATCGTCCCCAACCACCCATAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGc                                                                                              <  1:108459/249‑1 (MQ=255)
                                                                                                                                           ggcgggcgTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCg                                                                                             >  1:322436/1‑250 (MQ=255)
                                                                                                                                                 ggtaagggaaagaccccccaGGATTTGGGTGGAGAACCAACATTTTGGATTAGC‑CCATGGAGACCCCCAGTGCAC‑GAATAATCGCCCCCAACCATCAAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGa                                                                                      <  1:413529/234‑1 (MQ=255)
                                                                                                                                                    aggcttaaaaaCCACCAGGACTGGGGTCGAGAAACGACTTTTTGGCGTATA‑CCACCGAGACCCCCAGTGCAC‑CGATAATCGTCACAAACCACCAAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCt                                                                                   <  1:33954/242‑1 (MQ=255)
                                                                                                                                                    aagggtaaaAACCCCCAGGTCTTGGGGCGGGGAACGACTTTTTGGGGTAAC‑CCCTGGAGGGCCCCAGTCCCC‑CAATAATCCCCCCCAACCCCCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGc                                                                                    <  1:324216/246‑1 (MQ=255)
                                                                                                                                                                caccagcgtgtgggtgggagAACGCCATTTTGGTGTACC‑CCACCGAGAGCCCCGCAGCCC‑CGAGAAGCGCCCCCACCCAACCATAAATGCCACTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaa                                                                       <  2:423624/232‑1 (MQ=255)
                                                                                                                                                                     ggcttggggTCGAGGAACGACATTTGGGGGTATC‑CCACCGAGAGCCCCAGTGCCC‑CGAAAACCGTCACCACCCATCAAAAAAGGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                                  <  1:221001/245‑1 (MQ=255)
                                                                                                                                                                                 agcaacaaCATTTTGGCATATC‑CAAGCAAGACCCCCAGTCCAC‑AAATAATCTACACCAACCATACAAAAATGGTAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGGTAGCTTCGTGACAATAAATTACGACATAGCATAc                                                      <  2:376129/244‑1 (MQ=255)
                                                                                                                                                                                                                     cAGTGCCC‑CGATAACCGTCCACACCCAACCAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggg                   <  1:152707/251‑1 (MQ=255)
                                                                                                                                                                                                                       ggcccc‑cGAAAATCGCCACCAACCAACCATAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGtt                 <  1:478889/246‑1 (MQ=255)
                                                                                                                                                                                                                              cGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCt           >  2:34711/1‑251 (MQ=255)
                                                                                                                                                                                                                                 aaaTCGTCCCCAACCATCCATAAATTCCATCCCCGCCAGTAGAGGCCAGGTTAGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCAGCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGAt        <  1:57547/250‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGTCCCCAACCATCCATAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGcc     <  1:34711/250‑1 (MQ=255)
                                                                                                                                                                                                                                        cACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGtt  >  1:123833/1‑250 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            
AATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATA‑CCATCGAGAGCCCCAGTGCAC‑CGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTT  >  NC_000913/4336658‑4337137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑