| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,185,199 | 0 | T | G | 26.9% | 181.2 / 13.6 | 95 | T35P (ACC→CCC) | thiM | hydroxyethylthiazole kinase |
| Reads supporting (aligned to +/- strand): ref base T (15/53); new base G (25/0); total (42/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGC > NC_000913/2184952‑2185449 | aaaaGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTTAAGGGTTg > 1:208252/1‑251 (MQ=255) aCAAAAATGGCGGCGATAATCGAGCGACCCCACCGCTACTGGATCAAGCGGCCCGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCTCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTTTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑catcat < 2:10882/251‑1 (MQ=255) aaaaTGGCGGCGATAATCGAGCCCACCCCTCGCTACTGGATCATGCGTCCAGGGGGTTTGAGAGCTTTTTGCTTGCTTAACGGCAGCACTCATCGCCTGAGCGCTTGGCTTCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTGCGGTTTCGATAGCCATCTCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTgg < 2:313681/251‑1 (MQ=255) gggcggcGATAATCGAGCGCACCCACCGCTACGGGATCAATCGTCCAGGGTGTTTGATAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCAGCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCAt < 1:391651/250‑1 (MQ=255) gcgATAATCGAGCTCAACCACCGCTACGGGATCAAGCGTCCGGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAATGCACTGGCGATAGCCGCAAAATGACTGGCCTCTGCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGt < 2:88380/251‑1 (MQ=255) cgATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTTCCCCA‑CCTCATTTG‑Tccatcaggg > 1:260035/1‑243 (MQ=255) cgATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTg < 1:481413/251‑1 (MQ=255) gATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTTG‑TCATGCAATGc > 1:94458/1‑251 (MQ=255) aTCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTTGACCC‑CATCATTGG‑Tccctagcgtgccc > 2:42698/1‑238 (MQ=255) aTCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGGACCC‑AATCATTTG‑TCATGCCGTGgaca > 1:459091/1‑250 (MQ=255) cgggcgcACCCACCTCTACTGTAATAAGCGGCCAGGGTGTTTGAGAGCTTTTTGCTTTCTCAACGTCAGCACGCACTGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGTCCTCTTCGGGTTCGATAACCATCGCTGTCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTGTTCACCA‑CATCATTGG‑TCATGCAGTGCACAag‑a < 2:408634/248‑1 (MQ=255) cGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAag < 1:310089/250‑1 (MQ=255) aGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTGGCACCA‑CACCATTGG‑GCATGCAGTGAACAAG‑Agg > 2:260728/1‑251 (MQ=255) cgcACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTg < 2:240814/240‑1 (MQ=255) cgcACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CCTCATTTG‑TCATGCAGTg > 1:240814/1‑240 (MQ=255) ccccaccGCTACTGTATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTTCTTGCTCAACGTCAGCACGCAACTCCGGCGCTCGTGGCTGCGTCAGTGTGCCAACGTTAAGCAACAAGGCATTGGCGATAGCCGCAAACTGACTGGCCTCTTTGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCCAGGTATTGGCGGTATAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACCAG‑AGGGG‑AAt < 2:390886/250‑1 (MQ=255) cccGCTCCTGGATCGAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCCCCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGTGG‑AATGTTg < 1:305508/250‑1 (MQ=255) accGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCC‑CATCCTTGG‑TCATGCAGTGCACAAG‑AGGGG‑AAAGttt > 1:267635/1‑250 (MQ=255) ccGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTgg > 1:381441/1‑251 (MQ=255) cGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCATTTG‑TCATGCAGTGCACAAG‑AGGGG‑GATGtttgg > 1:203855/1‑248 (MQ=255) cTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCCTCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGGAAAGGGTTGTACCC‑CATTACTTG‑TCGTGCAGAGCACACG‑AGGGG‑AATgattgtgg > 2:242144/1‑244 (MQ=255) cTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATAATTGG‑TCATGCCATGCACAAG‑AGGGG‑GATGTTgggg > 1:183003/1‑248 (MQ=255) cAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGgtgt < 1:413265/251‑1 (MQ=255) aaGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCCCCAACA‑CATTGG‑TCATGTAGTGGGAAAG‑AGGGGGAAGGTTGGGG‑aaaaggggg > 1:456231/1‑245 (MQ=255) aGCGTCCAGGGTGTTTGAGATCTTTTTGCTTGCTCAACTGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTCACAGTTTTCACCC‑CCTCATTTG‑TCATGCAGTTCACCAC‑AGGGG‑AATTTTTGTG‑gaaaaatgtta > 2:231569/1‑244 (MQ=255) ccAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTTG‑TCATGCCGTGCACAAG‑AGGGG‑GATGTTGGTG‑AAAAAGGTGTAAcgcgg > 1:506239/1‑250 (MQ=255) ccAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAAcgcg < 1:185072/250‑1 (MQ=255) gggTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTgcg < 1:449110/251‑1 (MQ=255) gagaGCTTTTTGTGTGCTCAACGGCAGCCCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTgc < 2:94458/251‑1 (MQ=255) gagaGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCCTTGGGTC‑TGCAGTGCATAAG‑AGGGG‑GGTGTTGGGG‑GAAAAGGGGTAAcgcgggggcaagatgg > 2:219345/1‑239 (MQ=255) gagCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCCTTGG‑GCATGCAGGGCACAAG‑AGGGG‑GGTGTTGGTG‑AAAAAGGGGTAACGCGGGCGCCGATTgcgc > 1:48572/1‑251 (MQ=255) agCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGTG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGCt > 1:208732/1‑251 (MQ=255) agCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGGAAAAGGTTGCAACA‑CAACATTGG‑TCATGCAGTGGACAAG‑GGGGG‑AATGTTGGGG‑AAAAAGGGGGAACGCGGGCGCAGAATGCGCt > 1:213610/1‑251 (MQ=255) ttttGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGaa < 1:391845/250‑1 (MQ=255) tttGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAAGGTTGCACCA‑CATCATTGG‑GCCTTCAGTGGCCAAG‑AGGGG‑GAGGGTGGTG‑GAAAAGGGTTCACCCGTGCGCAGATTacgccggacc > 2:257990/1‑245 (MQ=255) tgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCATTGG‑TCATGCAGGGCACAAG‑AGGGG‑GATGTTGGGG‑AAAAAAGGGTAACGCGGGCGCAGATTGCGCTGAACCcagc > 1:200336/1‑249 (MQ=255) tgctCAACGGCAGCACGCATCGCCTCCGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGGAAAGGCTTGCAACC‑CATCATTGG‑GCATGCAGTGCCCAAGCAGGGG‑A‑TGTTGGTG‑AAAAAGGGGTTACGTGTGCGCAGGTTGCGCTGCACCcagc > 1:152357/1‑249 (MQ=255) gctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCcagcag < 1:278562/250‑1 (MQ=255) ctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTTCACCA‑CATCATTTG‑TCATTCAGGGCACAAG‑AGGGG‑AATGTTGGGG‑GAAAAGGGGTAACGCGGGCGCAGATTGCGCTGAACCCAGCAggg > 1:200538/1‑250 (MQ=255) tCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGACTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTGAAGAGTTGCCCCC‑CCTCATTTG‑GCATGCCGCGCACAAG‑AGGGG‑ACAGGTGGTG‑GAAAAGGAGTAACGCCTgcgccgaatgtccttaaacccgcatgtc > 2:138412/1‑227 (MQ=255) cAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCg < 1:18993/251‑1 (MQ=255) aaCGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTTG‑TCATGCCGGGCACAAG‑AGGGG‑GATGTTGGGG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAgggcg > 1:398272/1‑247 (MQ=255) cGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACt < 1:501910/251‑1 (MQ=255) agcaCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAAGTGTAACGCGTGCGCAGATTGCGCTGCAACCAGCAGGTGGACtttc > 1:276702/1‑248 (MQ=255) agcaCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGTG‑AAAAAGGTGTAACGCGGGCGCCGATTGCGCTGAACCCAGCAGGGCGACTTGc > 1:317841/1‑250 (MQ=255) cGCCTGAGCGCGTGGCTGAGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCt < 1:12429/251‑1 (MQ=255) gCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCCTTGG‑GCATGCAGTGCACAAG‑AGGGG‑GATGTTGGGGGAAAAAGG‑GTAACGCGTGCGCAGATTGCGCTGGACCCAGCAGGTCGACTTGCATAGTTTGCTc > 1:352913/1‑251 (MQ=255) gCCTGAGCGCGGGGCTGCGTCAGTGTGCCAACGTTAATCAATAAGTCACTGGCGATGGCCGCAAACTGACTGGTCTCTTCGGTTTCGATAACCATCGCTTGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATGTTTTGCTc < 2:345077/251‑1 (MQ=255) ccTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCt < 2:207981/249‑1 (MQ=255) gcgcGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGAAAAGGGTTGCACCC‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGGG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGACTTGCATAGTTTGCTCCTGccc > 1:3494/1‑250 (MQ=255) ggTGGCTGCGTCAGTGGGCCAACGTTAAACAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCCCCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATaa < 2:317841/250‑1 (MQ=255) gCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAg < 1:22148/231‑1 (MQ=255) gCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGGAAAGGGTTTCCCCA‑CCTCATTGG‑TCATGCAGGGCCCAAA‑AGGGG‑GGTGTTGGTG‑GAAAAGGGGTAACGCGTGCGCAGATTGCGCGGAACCCCGCAGGGCGACTTGCATAgaggacgggagcgcggcgggt > 2:22148/1‑231 (MQ=255) gCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGa < 1:223039/250‑1 (MQ=255) ccAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGAcc < 1:390214/251‑1 (MQ=255) ttAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGGAAAGGGTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGTG‑AAAAAGGGGTAACGCGTGCGCAGATTTCGCTGAACCCCGCAGGTCGACTTGCATAGTTTTCTCCTGCCCTAACGGGAAGGA‑GCAATGAACtggtgg > 1:397717/1‑251 (MQ=255) aTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGTCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGGG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTTGTGGTcc < 2:278278/251‑1 (MQ=255) aTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTcc < 1:471799/251‑1 (MQ=255) acaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGa < 1:257990/251‑1 (MQ=255) aaaGGCACTGGCGATAGCGGCAAACTGACTGGCCTCGTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATGGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGAGCTGGTGGTCCGTGAc < 2:231364/250‑1 (MQ=255) gCACTGGGGATAGCCGCAAACTGACGGGCCTTTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCGGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTcc < 2:87936/251‑1 (MQ=255) ggCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCATTGG‑GCCTGCAGGGCACAAG‑GGGGG‑AATGGTGGGG‑AAAAAGGTGTAAAGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGACTTGCATAGTTTGCTCCTGCCATAAAGTGAAGAA‑GCAATGACCTTGTGGGACGTGACTTcccctcc > 1:99811/1‑247 (MQ=255) gATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCACGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGGG‑GAAAAGGTGTAACGCGTGCGCCGATTGCGCTGAACCCCGCAGGTCGACTTGCATAGATTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTg > 2:221970/1‑251 (MQ=255) aTAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCA‑CATCATTTG‑TTATGCAGAGCACAAG‑AGGGG‑GATGTTGGTG‑AAAAAAGGGTAACGCGGGCGCAGATTGCGCTGAACCCAGCAGGGCGACTTGCATAGTTTGCTCCTGCCCTAACGGGAAGAA‑GCAAAGACCTTGTGGGTCGTGACTTTCCTACgacgg > 1:303037/1‑247 (MQ=255) cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATc < 2:200538/251‑1 (MQ=255) tgGCCTCTTCGGGTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGGGGTCGGTGACTTCCCTACGCGGGCATTATCCAGATCAg < 2:456231/250‑1 (MQ=255) tgCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAAGCTTGCCCCA‑AATCAATGG‑GCATGCCGGGCACAAG‑GGGGG‑AAAGGTGGGG‑AAAAAGGGGTAAAGCAGGCGCAGATTTCGCTGAAACAACCAGGGGGGCATACATAGTTTGCTCCCGCCCTAAAGTGAAGAAAGCAAGAACCTG‑AGGTCCGGGACTTCCCTaacccagcactttcccacaaaggg > 2:371243/2‑228 (MQ=255) gCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CCTCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GAAGTTGGTG‑AAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCCTAACCTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCCGGCATTATCCAGATCAgggg > 1:297023/1‑249 (MQ=255) ccTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGa < 2:242631/251‑1 (MQ=255) ctctTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCGCCA‑CATCATTGG‑TCATGCAGGGCACAAG‑AGGGG‑GATGTTGGGG‑GAAAAGGGGTAACGCGTGCGCAGATTGCGCTGGACCCAGCAGGGCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGAACTGGTGGTCCGTGACTTCCCCACGCTGGCATTATCCAGATCAGGTGAt > 2:169845/1‑251 (MQ=255) gttttCGCTAACTATCGCTGGCGATGCACCGCGCGCCAGCAAGGTTTTGGCGGTAAATGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACACG‑AGGTG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTGGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGCTCATGTGATACGGGt < 2:141784/249‑1 (MQ=255) gtCGATAACCTTCGCTGGCGATGCATCGAGCGTCAGCATGGTTTTGGCGGTAAAGGTTTGCACCA‑CATCAGTGG‑TCATGCAGTGCACAAG‑AGGTG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTGGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGTTCCTTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTag < 2:381441/249‑2 (MQ=255) tCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAtt < 1:30742/250‑1 (MQ=255) acGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCCCAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATATTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACGTCCCTACGCTGGCATTATCCAGATCAGGGGATACGGGTAtt < 1:222185/249‑1 (MQ=255) cGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAttt < 1:8126/250‑1 (MQ=255) aTAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtctc < 1:321734/251‑1 (MQ=255) aaCCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CCTCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTTGGCTGAAACCAGCCAGTCGACTTGCATAGTTTGCTCCTGCCCTAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCCGGGGATACGGGGATTTCTCa > 1:165479/1‑250 (MQ=255) aaCCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTAGTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGTCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtctaag < 2:207312/251‑4 (MQ=255) cccATCGCTGGAGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCAACA‑CAGCATGGG‑TCATGCAGGGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGAAACGCGTGCGCAGATTGGGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGGCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGc < 2:208252/250‑1 (MQ=255) aCCATCGCTGGCTATGCACCGAGCGCCCGCATGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGTCTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGCCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTAATACGGGTATTTCTCAGc < 2:10834/251‑1 (MQ=255) cGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCAATGG‑TCATGCAGTGCACAAG‑AGGGG‑GATGTTGGTG‑GAAAAGGGGTAACGCGGGCGCAGATTGAGCTGAACCCAGCAGGGCGACTTGCCTAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCCGGGGGTTCCTGACTTCCCTACGCGTGCCTTATCCAGATCACGGGATTAGGGGATTTCTCCGCCTTcc > 1:493831/1‑250 (MQ=255) ggCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCCCAA‑AATAACTGG‑TCATGCAGGGCACAAG‑AGGGG‑GATGTTGGGG‑AAAAAAGTGTAACGCGTGGGCAGATTGCGCTGAACCCAGCAGGGCGAATTGCATAAGTTGCTCCTGCCATAACGTGAAGAA‑GAAAAGACCTGGGGGTCCGTGGTTGCCCTACCCATGCATTATCCAGATCAGGTGAGACGGGGATTTCTCAGCCTTCACGc > 2:503577/1‑250 (MQ=255) ccGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAc < 1:93188/251‑1 (MQ=255) ggcgcCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAcccc < 2:506239/250‑1 (MQ=255) cgcCAGCAAGGTATTGGCGGTAAAGGTTTTCACCA‑CAACCTTTG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCCCGCAGAAGGGCCCCCCg > 1:104162/1‑250 (MQ=255) cAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTc < 1:141604/251‑1 (MQ=255) gggTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatg < 2:3494/250‑1 (MQ=255) cGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatg < 1:176586/251‑1 (MQ=255) tAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTgaag < 1:154249/134‑1 (MQ=255) tAAAGGTTTGCACAA‑CATCCTTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGCTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTgaag > 2:154249/1‑134 (MQ=255) aaagTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGGCTGGCATAGTTGGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCATACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCGTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATaaa < 2:459091/248‑1 (MQ=255) aaGGTTTGCACCA‑CATCATTGG‑TCATGCAGTTCAGAAG‑AGGGG‑AAGGTTGGTG‑AAAAAGGTGTAACGCGTGCTCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGGCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTGCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTGGCGATGATaaa < 2:166938/251‑1 (MQ=255) aGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAt < 1:106417/251‑1 (MQ=255) aGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAt < 2:200336/251‑1 (MQ=255) gTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTGCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCg < 2:495093/251‑1 (MQ=255) tttGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGc > 1:162675/1‑251 (MQ=255) | AAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCA‑CATCATTGG‑TCATGCAGTGCACAAG‑AGGGG‑AATGTTGGTG‑AAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAA‑GCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGC > NC_000913/2184952‑2185449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |