Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA45.5% 13.3 / 12.3 22intergenic (‑119/+84)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (11/1);  new base A (0/10);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 3.40e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT  >  NC_000913/1824812‑1825300
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
ggatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGagaaa                                                                                                                                                                                                                                                     >  1:471073/1‑248 (MQ=255)
  atgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaagaaa                                                                                                                                                                                                                                                <  1:476166/251‑1 (MQ=255)
      aaTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGTTGGTAACGTGCAAATTTCGCACGTTTCACCGGCAGCATTTCGCCAAAGATTAACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGACAGTTATTTTAATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTTCAG‑GGTGATCA‑GGCTTTTTTTACAA‑CTGTTTtagcagagaacggcacaccc                                                                                                                                                                                                                                            >  2:145141/1‑241 (MQ=255)
                             cACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGCGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CCGTTTGAGAAGCGAAAAGAAAACCCCCGATCCTGTCCCCCGCATTACt                                                                                                                                                                                                                     >  2:311195/1‑251 (MQ=255)
                                gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACCGATTCACCGACATTTTACGCATATCAAAGTATTCCCTACTCGCTCCCAGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTTG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑TGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAATACAACCGCCTATCCTGGCCAGCGCATTACttc                                                                                                                                                                                                                   >  2:279968/1‑248 (MQ=255)
                                          tGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCTGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCCAGGTAGTgg                                                                                                                                                                                                         >  1:479312/1‑250 (MQ=255)
                                            ggAACGTGCAAATTTCTCGCGTTTCACCGGCAGCCTTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGAGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAACAGACAACCGCCGTTCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                                                                                                                       >  2:194357/1‑250 (MQ=255)
                                                                                gtCGCCACAGATGCTCCGACTTTTTACGCATATAAAAGTTTTCCATCCCCTCCCCGGGCCCGTTCTTTGTATTGTTATCGTTAAGTTAACCGGGG‑AGGTTTATCGGCAACTGGGGTGATTTACGTT‑AGGGGTGATCA‑GTTTTTTTTTACAA‑CTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                                                                                                                   <  1:277492/249‑1 (MQ=255)
                                                                                                          cacatataaaatttttCCATACTCGCCCCGGGCCCTTTTTTTTCATTGTTATGGTTGAGGTACCGGTGG‑ATGTTTATCGGCAACTGGGTTGATTTACTTT‑AGTGGTGATCG‑GGTTTTTTTTACAA‑CTTTTTGATAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACCGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCtt                                                                                                                                       <  1:149948/237‑1 (MQ=255)
                                                                                                            cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCAGAAAGATTATTtagccactgccaggatttttactttt                                                                                                                                      >  2:320084/1‑229 (MQ=255)
                                                                                                                      tcttCCATACCCGCCCCCGTCCCTTTTTTTTATTTTTTATCTTTGAGGTA‑CCGTGGGATGGTTTTCGGCAACGGGGGTGTTTTACGTT‑ATTGGTGATC‑‑GGGTTTTTTTAAAATCTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGa                                                                                                                            <  2:227401/248‑1 (MQ=255)
                                                                                                                          caatccTCGCCCCCGCCCTGTGCTTTTCTTTGTTATCGTTCAGGTACCGGGGG‑AGGGTTATCGCCAACTGGGGTGATTTCCGTA‑GGTGGTTA‑CACGGCTTTTTTTACAA‑CTGTTGGAAAAGAAAAAAGAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTc                                                                                                                       <  1:304116/246‑1 (MQ=255)
                                                                                                                           cATACCCGCTCCCGGCCCTTTCTTTTAATTTTTATCGTTCAGGTACCCGGGG‑ATGGTTAGCGGAACCGGGGGTTTTTTACTTT‑AGTGGTGATCA‑GTTTTTTTTTAAAA‑TTGTTAGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCt                                                                                                                      <  1:418165/251‑1 (MQ=255)
                                                                                                                              cctcccTCCCGGCCCTTTCTTTTTTTTTTTATGTTTCAGGTAACGGGGG‑TTGGTTATCGGCACCTCGGGTATTTTACTTT‑AGTGGTGATCA‑GGTTTTTTTTACAA‑CTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTc                                                                                                                   <  1:360482/246‑1 (MQ=255)
                                                                                                                               ccccccccgggCCCTTTCTTTTCTTTGTTTTCTTTCAGTTAACGGGGG‑ATTGTTACCGGCAACTGGGGTGTTTTATGTT‑AGTGGTGATCG‑GTTTTTTTTTACAC‑TTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCa                                                                                                                  <  1:119369/242‑1 (MQ=255)
                                                                                                                                  gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑Ac                                                                                                              >  1:190289/1‑251 (MQ=255)
                                                                                                                                         gCCCGATCTTTTCATTGTTATCGCTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGTTTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACAGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACAGGCGGTCTTAAGATTTTTGGCTGGAAGAATTTTTAGCAGTTGGAGGACATTT‑ACCTTTTTCCGCTGGTGGGTCTTTCAG‑ACGCctctt                                                                                                       >  2:174791/1‑250 (MQ=255)
                                                                                                                                                                 tcggttacccgggg‑ATGGTTACCGGCAACTGGGGTGTTTTACGTT‑AGTGGTGATCA‑GGTTTTTTTTACAA‑CTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCtttt                                                                                <  1:266543/238‑1 (MQ=255)
                                                                                                                                                                   aGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGCAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGAATATTCAGCCGGTGCAGG‑CATTTTACCCTTTGCCGCTGGGCGTTCTGTCAGGAC‑ATTCTCAAAATTAGCATTTAATTTCTTTTTCt                                                                             >  2:488694/1‑251 (MQ=255)
                                                                                                                                                                     ggtatcggtgaaTGGTTATCGGAAACTGGGTTGTTTTACTTT‑GGGGGTGATCG‑GGTTTTTTTTAAAT‑GTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTg                                                                            <  1:293681/240‑1 (MQ=255)
                                                                                                                                                                          ccgtgggtggtaaccggaacccggggTGATTTAGGTT‑GGTGGTGAGCG‑GGTTTTTTATACAG‑CGGTTTGAAAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCg                                                                       <  1:223828/228‑1 (MQ=255)
                                                                                                                                                                             gg‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAAATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTTTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGt                                                                   >  2:377952/1‑251 (MQ=255)
                                                                                                                                                                                         ggCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTTACTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTTTTTCGGCGTAAGGAtgttgt                                                        >  2:193159/1‑251 (MQ=255)
                                                                                                                                                                                                                                ttttttAAAA‑TTGTTTGAAAAGAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                    <  1:377952/250‑1 (MQ=255)
                                                                                                                                                                                                                                           cTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTTCGGCGTCAGGGTGTTGTAAATTTTGTTCTGGGTTTCCATGTTCGCCAGCATTTTAGCTTTgcgc         >  1:282179/1‑251 (MQ=255)
                                                                                                                                                                                                                                               ttgaaaaaagaaaaaaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGt      <  1:113463/242‑1 (MQ=255)
                                                                                                                                                                                                                                                taagaagaaaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGtt     <  1:104661/248‑1 (MQ=255)
                                                                                                                                                                                                                                                  agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTATTCAGTTTTTTGGCTGGAAGATTATTCAGCAGTTGGAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGGAAATTTTTTTCTAGGTTTCCCTGTGCGCCAGCATGTTAGCTTTGCGCTGttctt  >  1:268550/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      gagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCt                                                                                                           >  1:17627/1‑142 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
GGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGG‑ATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCA‑GGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTT  >  NC_000913/1824812‑1825300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑