Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,036,927	0	T	A	25.9%	107.6 / 11.8	55	L60Q (CTA→CAA)	hyaF	protein HyaF
Reads supporting (aligned to +/- strand):  ref base T (31/9);  new base A (0/14);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 2.52e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACA  >  NC_000913/1036679‑1037142                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                         gTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGAGAAACTGTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGACCGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GCGTTACACCATCAAC‑Taa                                                                                                                                                                                                                        <  2:109369/251‑1 (MQ=255)       aCCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAAc                                                                                                                                                                                                                  <  1:92496/251‑1 (MQ=255)              ggCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAAcc                                                                                                                                                                                                           <  2:230704/251‑1 (MQ=255)              ggCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAAcc                                                                                                                                                                                                           >  1:233670/1‑251 (MQ=255)                 ggAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAAccgcc                                                                                                                                                                                                        <  1:222625/251‑1 (MQ=255)                  gAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAAccgccg                                                                                                                                                                                                       >  1:305448/1‑251 (MQ=255)                        aTAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTATCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCCAC‑TAAGCGAACGCCAACCGCCGTTGGGc                                                                                                                                                                                                 >  2:499677/1‑251 (MQ=255)                          aaaCCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑GTTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑t                                                                                                                                                                                                                          >  2:331757/1‑224 (MQ=255)                          aaaCCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑t                                                                                                                                                                                                                          <  1:331757/224‑1 (MQ=255)                                       gggCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGTCCTGGAG‑CAATGTGCTCA‑CAGCCCGAAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGTTGCTGG‑CAGTc                                                                                                                                                                                 >  1:103128/1‑251 (MQ=255)                                       gggCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTc                                                                                                                                                                                 >  1:326231/1‑251 (MQ=255)                                        gttttgccGAACCGCGGCAGGGGCGTGCCTAATGAG‑GAAAATTTTTTCCTGTCTGCGGGGGCGA‑GGGCCGCAACCGAACGATGACGGTTTCAGA‑ATAACCCCCC‑GGCCAATCACCTGTCAGG‑AGAAGGATGAACCG‑TTTAGGG‑CGCCCCTGAAG‑CAATGTGCTCA‑CCCCCCGCAGTTG‑ATTGCGTTGTAAAAC‑GAGTTACAACAACAAC‑TAGGCAAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCg                                                                                                                                                                                <  1:115086/244‑1 (MQ=255)                                          cTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGAt                                                                                                                                                                              >  1:57644/1‑251 (MQ=255)                                                gAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGtt                                                                                                                                                                         >  1:41799/1‑250 (MQ=255)                                                gAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGtt                                                                                                                                                                         >  1:404208/1‑250 (MQ=255)                                                gAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGATGGGCGAGGTGCTGG‑CAGTCGATCTGtt                                                                                                                                                                         >  2:228540/1‑250 (MQ=255)                                                  cccgcggggggAGCGGGCCTCAGGAGC‑AAATTTTTTTCCCATCGGGGGGGGCCA‑GGAACGCACCCGAACGATGACTGTTCCAGC‑AGGACTCCCC‑GCCCAATC‑CCTGGAAGGGTGAATGATGACCCG‑ATTATGG‑GGGCCCTGGAG‑CAATGGGCTCA‑CAGCCCGCGGGGG‑ATTGGGCTGTTAACT‑GGGTTACATCATAAAC‑AAAGCGACCCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTaaa                                                                                                                                                                      <  1:410038/242‑1 (MQ=255)                                                     gcagcaGGAGCGTGCCTCATGAGCGAAATTTTTTCCC‑ATTGGCGGGGGCCA‑GGAACGCAACCGAAGAATGACAGTTTCAGA‑ATGAACCCAC‑TGCCGACCCCCTGTAAGG‑TGAATGATAAACCG‑ATTAGGG‑CGGCCCTGGAG‑CATTGTGCCCA‑CAGCCCGGAGGTG‑ATTGCGCTGTTAACC‑GAGTAAAAACATAAAC‑AAAGCAAACGCAAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAAtct                                                                                                                                                                   <  1:239728/251‑1 (MQ=255)                                                       agaagGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGCACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGACTGATGAACCG‑AGTATGG‑CGGCCCTCGAG‑AACTGTGCTCA‑CAGCAAGCAGTGG‑ATTGCGCTGTTAAAC‑GAGTTACCACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGACA‑TCGATATCTTAAAtctc                                                                                                                                                                  >  2:213388/4‑250 (MQ=255)                                                             ggCGTGCCTAATGAGCAAATCTTTTTTCC‑ATCTGGGGGGGCCA‑GGAACGCACCCAAACGTTGACAGTT‑CAGCCATGAACCCAC‑GCCCGATCCCCTTTCGGG‑TAAATGAAGACCGG‑AGTGGGG‑GGCCCCTGGAC‑AAATGTGCTCA‑CGCCCCGCGGGTG‑TTTGCCCGGTAAAAC‑GATTTAAAACACAAAC‑AAAGCAAACGCAAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCcg                                                                                                                                                           <  1:35313/250‑1 (MQ=255)                                                                gTGCCTCATGGGC‑AAATTTTTTTCCCATCTGC‑GGGGCCAAGAAACGCACCGGAAAGATGACGTTTTAAGC‑ATGAATCCCC‑GGCCGTTCGCTTGTCAGG‑TGAATGATGACCGG‑AGTAGGG‑CGCCCCTG‑AGACAATGTGCTCC‑CACCCCGCAGTTC‑TTGGCGCTTTAAAAC‑GAGTTACAAAATAAAC‑AAAGCAAGCGCAAGCCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCcgacg                                                                                                                                                        <  1:288817/251‑1 (MQ=255)                                                                gTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCC‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑AGACGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGA‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑CATGCGCTGTTAAAC‑GAGTTACAACCTCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGAGGCTGG‑CAGACGATCTGTTAAATCTCacacccgacg                                                                                                                                                        >  2:148989/1‑242 (MQ=255)                                                                    cccaggAGCAAAATTTTTTCCC‑ATCGGCGGGGGCCA‑GGAACCCAACCGAACGATGACAGTTTCAGA‑ATGAACCCCC‑TGCCGACCCCCTGTAAGG‑TGAATGAGGACCCG‑AGTAGGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTTTAAAAC‑GATTTACAACATCAAC‑TAAGCGACCGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCg                                                                                                                                                    <  1:38564/246‑1 (MQ=255)                                                                    cTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCg                                                                                                                                                    >  2:90032/1‑251 (MQ=255)                                                                      cATGAGCGAATTTTTTTTCC‑ATCTGCGGGGGCGA‑GAAACCCAACCGAACGATGACAGTTTCAG‑‑AAGAACCCCCCTGCCGATCCCCTGTAGGG‑TGAATGAGGACCGG‑GGTTTGG‑GGCCCCGGGAG‑AAATGTGCCCA‑CAGCCCGGGGGTG‑ATGGCGGTTTAAAAC‑GAGTTAAAACATCAAC‑AAAGCGACCGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCg                                                                                                                                                    <  1:26026/249‑1 (MQ=255)                                                                      cATGAGCAAAACTTTTTCCC‑ATCGGCTGGGGCCA‑GGAACGCAACCGAACGATGAAATTTTCAGC‑ATGAACCCAC‑GGCCGACCCCTTGTCAGG‑TGAATGATAAACCG‑AGTAGGG‑CGGCCGTGGAA‑AAATGTGCTCA‑CACCCCGCAGGTG‑ATTGCGCTGTAAAAC‑GATTAACAACA‑CAACCAAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTc                                                                                                                                                  <  1:65656/251‑1 (MQ=255)                                                                          aGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGGCGATCTGTTAAATCTTAACGCCGCAGATCGTCACtt                                                                                                                                              >  2:339793/1‑251 (MQ=255)                                                                                    tttccc‑ATCTGCGGGGGCCA‑GGAACGCAACCGAACGAGGAAAGTTTCAGC‑ATGAATCCCC‑TGCCGATCCCCTGTGAGG‑GGAATGAGAAACCG‑AGTAGGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCGGGTG‑ATTGCGCTTTTAAAC‑GAGTTACAACACCAAC‑TAAGCGAACCCAAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATAc                                                                                                                                     <  1:28864/246‑1 (MQ=255)                                                                                     ttccc‑ATCTGGTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTACAGC‑ATGAACCCCC‑TGCCAATCACCTGTCAGT‑TAAATGATGAGCCG‑AGTATGG‑CGGCCGTGGAG‑AAATGTGCCCA‑CAGCCCGCAGGTG‑ATTGCTCTGTAAAAC‑GATTTAAAACACCAAC‑AAAGCGACCGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACg                                                                                                                                    <  1:144394/247‑1 (MQ=255)                                                                                      ttCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCt                                                                                                                                  >  1:391140/1‑251 (MQ=255)                                                                                       ccc‑ATCTGCTGGGGCAA‑GGAACGCAACCGAACGATGAAATTTTCAGC‑ATAAACCCCC‑TCCCAATCCCCTGTAGGG‑TGAATGATGACCCG‑ATTATGG‑CGCCCCGGGAG‑AAATTTGCCCC‑CACCCCGCAGGTG‑ATTGCGCTGTAAACC‑GAGTTACAACATCAAC‑AAACCGAACCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCtt                                                                                                                                 <  1:451270/250‑1 (MQ=255)                                                                                                                 aaCCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGCAGGG‑CGGCCCTGGAG‑CAATGCGCTCA‑CCGCCCGCAGGAG‑ATTGAGAAGTTAAAC‑GAGTTAAAAAATCAAA‑TAAGCAAACAACAACCGCCGTTTGGCGAGGTGCTGG‑CAGTCGATCTATTAATTCTCAACGCCGCAGATCGTCACTTTATAAAAACGCTTCTCGGGGAAGGGGGAGTGTCAGTg                                                                                                         >  2:431963/1‑251 (MQ=255)                                                                                                                  aCCGAACGCTGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTgc                                                                                                        >  1:417516/1‑251 (MQ=255)                                                                                                                          aagactgTTTCAGC‑ATGAATCCAC‑TGCCGATACCCCGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGGG‑CCATGTGCTCA‑CACCCCGCAGGTG‑ATTGCGCTGTTACAC‑GAGTTACACCCACAAC‑TAAGCGAACGCCCACCGCCGTCGCGCGAGGTGATGC‑CAGTCGATTTGTTAACTCCAAACGCCGACGATCGTCACCTTATCACTACGCTTCTCGGGGCAGGAGAAGTGTAAGGCCGCATTc                                                                                                  >  2:30437/7‑249 (MQ=255)                                                                                                                             agaGTTTCAGT‑ATGAACCCAC‑TCCCCATCACTTGTAGGG‑GGATTGAT‑AACCGGAGTAGGG‑CGGCCCTGAGG‑AAATGTTCTCA‑CAGCCGGCAGGTG‑TTTGGGTTTTTAAGA‑GAGTTACACCAACAAC‑AAGGCAAACGCAAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTcagcag                                                                                             <  1:339793/249‑1 (MQ=255)                                                                                                                              caatttccagaataaacccAC‑GGCCGATCCCCTGTCGGG‑TAATTGATGAACCG‑AGTGTGG‑CGCCCCTGGAG‑CAATGTGCCCA‑CCGCCCGGAGGTT‑ATGGCGCTTTTAACC‑GATTAACAACACCAAC‑AAAGCAACCGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTcagcag                                                                                             <  1:268596/234‑1 (MQ=255)                                                                                                                               aGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGCTCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGc                                                                                           >  2:436311/1‑251 (MQ=255)                                                                                                                                gTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCt                                                                                          >  1:455098/1‑251 (MQ=255)                                                                                                                                      gC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCCGTGCGCATTCAGCAGGCTgacgac                                                                                    >  1:154151/1‑251 (MQ=255)                                                                                                                                            aaTCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACagtg                                                                                >  1:367101/1‑250 (MQ=255)                                                                                                                                                              cTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACaggagg                                                              >  1:330076/1‑251 (MQ=255)                                                                                                                                                                             gatgaccgaagtctGG‑CGGCCCGGGAG‑CAATGTCCTCA‑CGGCCCGC‑GGTGGCTTGCGGTTTAAAAC‑AATTCACACAAACAAC‑AAAGCAAACCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCgg                                                 <  2:233670/238‑1 (MQ=255)                                                                                                                                                                             atgaACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCgg                                                 >  1:343726/1‑250 (MQ=255)                                                                                                                                                                              tgaACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGCCGATCGTCACTTTATCAATACGCCTCTCGGGGAAGGGGAAGTGTCAGTGCGCCTTCAGCAGGCTGACGACAGTGAAAGGGAAATACAGGAGGGGATCCTCTGCGGAt                                               >  2:383755/1‑251 (MQ=255)                                                                                                                                                                              agaccgg‑AGTATGG‑CGGCCCTGGAG‑CAA‑GGGCCCAACGGCCCGCGGGTG‑ATTGCGCTTTAAAAC‑GAGTAAAAAAACAAAC‑AAAGCGACCCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGAt                                               <  1:290188/245‑1 (MQ=255)                                                                                                                                                                                gcaatcaGTATGGGCGGCCCGGGAG‑CATTGTCCTCA‑CAGCCCGCATGTG‑TTTGCGCTGTTAAGC‑GAGTAACAACACAAAA‑GAAGCAAACCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGAt                                               <  2:367101/245‑1 (MQ=255)                                                                                                                                                                                  ccG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATg                                           >  1:120226/1‑251 (MQ=255)                                                                                                                                                                                         aTGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAACC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCgggg                                     >  1:147884/1‑250 (MQ=255)                                                                                                                                                                                         aTGG‑CGGCCCTGGAG‑CAATGCGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGGTGGGCGAGGTGCCGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACCTTATCAATACGCTTCTCGGGGAAGGGGAAGTGCCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGGGAAATACAGGAGGCGATCTTCTGCGGATTATGGCgggg                                     >  2:79799/1‑250 (MQ=255)                                                                                                                                                                                         aTGG‑CGGCCCTGGAG‑CAATGAGCTCA‑CAGCCCGGAGGGG‑ATTGCGCTGGTAAAC‑GAGATACAACAACAAC‑TAAGCGAACGCCAAACGCCGAGGGGCGAGGGGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTGTATCAAAACGCTTCTCGGGGAAGGGGAAGTGTCAGGGCGCAGTAAGCAGGCTGACGACAGTGAAAGGGAAATACAGGAGGCGAGATTCTGCGGATTATGGCgggg                                     >  2:63294/1‑250 (MQ=255)                                                                                                                                                                                                    gggAG‑CAATGTGCCCA‑CAGCCCGCGGGTG‑TTTGCG‑TTTTAAACCGAGTTACAACACCAAC‑AAAGCAACCCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGt                           <  1:403141/250‑1 (MQ=255)                                                                                                                                                                                                     ggAG‑CCATGTGCTCA‑CAGACCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGAGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGAGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAACGTGAAATACAGGAGGCGATCTTCTGCGGATTATGCCgggagtggggacgtg                          >  2:275678/1‑239 (MQ=255)                                                                                                                                                                                                               tgCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCgggggggcggagagcgggggagg                  >  1:227383/1‑230 (MQ=255)                                                                                                                                                                                                                   ca‑caGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTgcggggcaagcgcggggagagagt             >  1:65995/1‑230 (MQ=255)                                                                                                                                                                                                                    caccgCCCGCAGGGT‑ATTGCGCTGTTAACC‑GTGTCACAACATCAAC‑AAAGCGACCGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGtt             <  1:20606/247‑1 (MQ=255)                                                                                                                                                                                                                     ccaGCCCGCGGGTG‑ATTGCGCTGTAAAAC‑GAGTTAAAACATCAAC‑TAAGCCAACCCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTg            <  1:312712/250‑1 (MQ=255)                                                                                                                                                                                                                               ggTG‑ATTGCGCTGTAAAAC‑GATTTACAACACCACC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACa  <  1:132070/251‑1 (MQ=255)                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                         GTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAGCAGGAGCGTGCCTCATGAGCGAAACTTTTTTCC‑ATCTGCTGGGGCCA‑GGAACGCAACCGAACGATGACAGTTTCAGC‑ATGAATCCAC‑TGCCGATCACCTGTCAGG‑TGAATGATGAACCG‑AGTATGG‑CGGCCCTGGAG‑CAATGTGCTCA‑CAGCCCGCAGGTG‑ATTGCGCTGTTAAAC‑GAGTTACAACATCAAC‑TAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGG‑CAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACA  >  NC_000913/1036679‑1037142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑