Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	31 [0]	[0] 31	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	31 (0.510)	30/492	0.6	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

GCCGCTTTCCAGGTCGATAAACAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACACGTTTCACGCGCCCTTCCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACAAAGGCGATACGCTTAACCCTGCTTAACGAGAATGCACCCTCTTGTTTCATCGCCTTAACCGCATTGGCTGACTGAGCAAA  >  NC_000913/3077320‑3077815                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         gccgcTTTCCAGGTCGATAAACAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATAGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTTGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaa                                                                                                                                                                                                                                                        >  2:33795‑M1/1‑248 (MQ=255)                      cAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggg                                                                                                                                                                                                                                  >  1:386471‑M1/1‑227 (MQ=255)                              gTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaa                                                                                                                                                                                                                          <  2:502283‑M1/251‑33 (MQ=255)                                               ccatgaattcatGATCGGTAATTTCTGGCAGTTCAAATACGAGCAGACTGACATCCCGTTTTCCATGAATAGCTGCAACTTTGGTTTTCATTTTTTCCTCTGTAATCAGTGTTTATGGTTGTCGGTTAATTGATTAAATAAAGTGTCGATTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCATAaagcaattgtgcagggaaaccggtgtgttgaaaacagtgaatttttaat                                                                                                                                                                                                         <  2:272381‑M1/246‑50 (MQ=255)                                                               ggTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattt                                                                                                                                                                                         >  2:242906‑M1/1‑186 (MQ=255)                                                                   aTTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgtgcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagtt                                                                                                                                                                                     >  2:83057‑M1/1‑182 (MQ=255)                                                                        tGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacatttagttat                                                                                                                                                                                   >  2:438536‑M1/1‑177 (MQ=255)                                                                                              cGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacg                                                                                                                                                          <  1:33795‑M1/251‑97 (MQ=255)                                                                                               ggACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgc                                                                                                                                                         >  1:231929‑M1/1‑154 (MQ=255)                                                                                                     cccGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatc                                                                                                                                                    <  2:238773‑M1/250‑103 (MQ=255)                                                                                                           tGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatg                                                                                                                                              <  1:242906‑M1/250‑109 (MQ=255)                                                                                                                  aaTAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattac                                                                                                                                      >  1:353873‑M1/1‑135 (MQ=255)                                                                                                                           aaCTTTGGTTTTCATTTTTACCTCTGAAATCAGTGCTTATGTTCGGCGGTTAATTGATTAAATAAAGTTTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATGCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatcgatatatcaacaaatgtcaatacgcatatcgtgatgcatattacataattgat                                                                                                                             >  2:220883‑M1/1‑126 (MQ=255)                                                                                                                                    tttCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgc                                                                                                                    >  1:277368‑M1/1‑117 (MQ=255)                                                                                                                                    tttCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattg                                                                                                                     <  1:5694‑M1/250‑134 (MQ=255)                                                                                                                                    tttCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattg                                                                                                                     <  2:353873‑M1/250‑134 (MQ=255)                                                                                                                                       cATTTTTACCTCTGTAATCAGTTTTTATGGTCGGCGGTGAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGATTATCCGTCACAaagcaattgagcagagaaaccggtgttttgaaaacagtgaattattaatttctgatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttatttcat                                                                                                                  <  2:80635‑M1/250‑137 (MQ=255)                                                                                                                                        aTTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatat                                                                                                                <  2:277368‑M1/251‑139 (MQ=255)                                                                                                                                          ttttACCTCTGTACTCAGTGTTTATGTTCGGCGGTTAATTGATTAAATACAGTTTCGAGATTTGGATCGTCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttggaaacagtgaattcttaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcataatacataattgatatttattgcatata                                                                                                               >  2:195822‑M1/1‑111 (MQ=255)                                                                                                                                                    gTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtg                                                                                                     <  2:231929‑M1/250‑150 (MQ=255)                                                                                                                                                             gtTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaaca                                                                                           <  2:381925‑M1/251‑160 (MQ=255)                                                                                                                                                                                   aTTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccg                                                                     >  1:63476‑M1/1‑70 (MQ=255)                                                                                                                                                                                     taaataaaGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgc                                                                    <  1:411968‑M1/250‑183 (MQ=255)                                                                                                                                                                                                                        ttattaATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacgtatcaaggtaataaaaaag                                        >  1:100486‑M1/1‑33 (MQ=255)                                                                                                                                                                                                                        ttattaATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacgtatcaaggtaataaaaaag                                        <  2:100486‑M1/243‑211 (MQ=255)                                                                                                                                                                                                                         tattaATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaattgcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccga                                <  1:363430‑M1/250‑219 (MQ=255)                                                                                                                                                                                                                          attaATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaa                               <  1:83057‑M1/250‑220 (MQ=255)                                                                                                                                                                                                                                cAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacct                        >  2:45114‑M1/1‑25 (MQ=255)                                                                                                                                                                                                                                  atatCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgaccttt                      <  1:254026‑M1/251‑229 (MQ=255)                                                                                                                                                                                                                                    atCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctgttt                    <  2:184871‑M1/251‑231 (MQ=255)                                                                                                                                                                                                                                                       caaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataagtatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccgaaatgctaattac  >  1:88366‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         GCCGCTTTCCAGGTCGATAAACAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACACGTTTCACGCGCCCTTCCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACAAAGGCGATACGCTTAACCCTGCTTAACGAGAATGCACCCTCTTGTTTCATCGCCTTAACCGCATTGGCTGACTGAGCAAA  >  NC_000913/3077320‑3077815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.