Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,426	Δ1 bp	intergenic (‑16/‑216)	yhiN ← / → pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,426	0	G	.	100.0%	235.0 / NA	50	intergenic (‑16/‑216)	yhiN/pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (21/29);  total (21/29)

GCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTT  >  NC_000913/3637187‑3637670                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     gcatgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAaacggacg                                                                                                                                                                                                                                          >  1:484991‑M2/225‑248 (MQ=255) gcatgtgcacagcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcgacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAacggac                                                                                                                                                                                                                                           <  1:463827‑M2/26‑3 (MQ=255)       gcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAAt                                                                                                                                                                                                                                     <  1:223328‑M2/32‑1 (MQ=255)           atcaacaacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTa                                                                                                                                                                                                                                 >  1:25488‑M2/215‑250 (MQ=255)             caaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAg                                                                                                                                                                                                                              <  1:467323‑M2/39‑1 (MQ=255)                  acgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTcc                                                                                                                                                                                                                         >  2:494169‑M2/208‑251 (MQ=255)                    gacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTc                                                                                                                                                                                                                       <  2:239165‑M2/46‑1 (MQ=255)                      cgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTc                                                                                                                                                                                                                       >  1:15975‑M2/204‑249 (MQ=255)                           gtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTc                                                                                                                                                                                                                <  1:408353‑M2/53‑1 (MQ=255)                                     cgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCAt                                                                                                                                                                                                      >  1:419025‑M2/189‑251 (MQ=255)                                      gccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATc                                                                                                                                                                                                     <  1:38392‑M2/64‑1 (MQ=255)                                       gcgttttgaaaggtgacatgggttcggtgcagccttttgccgatgacggtatcgcccaggccggcgtgcggctcgggcatttgccgtgcttgccgacggaagattgagtctattaatgggtcctttggtgaacgagcaacaaagggccactagctcacctgattcctctcgaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGCCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCTGCATCa                                                                                                                                                                                                    <  2:5403‑M2/65‑1 (MQ=255)                                       ccgtgtcgaaaggtgacatgggtgccgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AACAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCCCCAGCATc                                                                                                                                                                                                     >  2:405793‑M2/187‑250 (MQ=255)                                           gttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAatat                                                                                                                                                                                                <  1:78403‑M2/69‑1 (MQ=255)                                                aaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACt                                                                                                                                                                                            >  1:249209‑M2/178‑250 (MQ=255)                                                aaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTa                                                                                                                                                                                           <  1:327395‑M2/74‑1 (MQ=255)                                                    tgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTaaa                                                                                                                                                                                         >  2:133419‑M2/174‑249 (MQ=255)                                                       catgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCGCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATTAATATTACTAAAAGGaa                                                                                                                                                                                    <  1:384374‑M2/81‑1 (MQ=255)                                                                 gtgcagcagtttgccgatgacgttatcgctcagcggggcgggcggcacgggcatttgcagtgcgtgccgaatgaagattgagtctattattgggtcctttggtgaacgatcaacaatgggccactagcacccctgattccgctctaaattccctatccgaGTTGCGTTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATGCATCAGTGGTCGCACCTGCCTCAATATTACTAAAAGTAAGTATTTGCCt                                                                                                                                                                          <  2:53585‑M2/91‑1 (MQ=255)                                                                        attttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagatttagtctatgagtgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctcgctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATtat                                                                                                                                                                   <  2:17818‑M2/98‑1 (MQ=255)                                                                                   gacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAat                                                                                                                                                         <  1:420035‑M2/108‑1 (MQ=255)                                                                                   gacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAat                                                                                                                                                         <  1:83949‑M2/108‑1 (MQ=255)                                                                                                   gcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCATCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTGTTGAGTGAAAt                                                                                                                                        <  1:173114‑M2/125‑1 (MQ=255)                                                                                                       cgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCAt                                                                                                                                    <  1:45242‑M2/129‑1 (MQ=255)                                                                                                            ggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAgg                                                                                                                               <  1:107594‑M2/134‑1 (MQ=255)                                                                                                              cacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAgggg                                                                                                                             <  1:343497‑M2/136‑1 (MQ=255)                                                                                                                 gggcatttgcagtgcttgccgaaggaagattgcgtctattattgggtcctgttgtgaacgatcaacaaagggccactagcacacctgattcctctctaaattccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGAACCAGCAACAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCa                                                                                                                          <  2:59611‑M2/139‑1 (MQ=255)                                                                                                                           agtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCaaaaaa                                                                                                                <  2:375456‑M2/149‑1 (MQ=255)                                                                                                                                  gccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaaggtccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGTACGTTAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCtat                                                                                                         <  2:278576‑M2/156‑1 (MQ=255)                                                                                                                                     gaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGCTTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGTCAAATCAAAAAAAGTCTATAtt                                                                                                      >  2:371760‑M2/93‑251 (MQ=255)                                                                                                                                     gaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATAtt                                                                                                      <  2:484991‑M2/159‑1 (MQ=255)                                                                                                                                        ggaagattgcgtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTc                                                                                                    >  2:233829‑M2/90‑250 (MQ=255)                                                                                                                                               tgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGc                                                                                             <  1:286857‑M2/168‑1 (MQ=255)                                                                                                                                                      attattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgcc                                                                                      <  1:133419‑M2/175‑1 (MQ=255)                                                                                                                                                           tgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGaa                                                                                 >  1:10172‑M2/71‑250 (MQ=255)                                                                                                                                                                           cgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATgcgc                                                                >  2:55395‑M2/55‑251 (MQ=255)                                                                                                                                                                                aacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGt                                                           <  1:278487‑M2/202‑1 (MQ=255)                                                                                                                                                                                         ccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGACAGTCACTGATAATGCGCCGCGTTCATGTCCt                                                  >  2:42972‑M2/41‑251 (MQ=255)                                                                                                                                                                                           actagcacagctgattcctctctaaataccctatcctaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGCGGTACCACCAGCATCAATATTACTAAAAGGAAGTATTGGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCTATACAGGGGGCAAATCAAACAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTc                                                 >  1:124682‑M2/39‑250 (MQ=255)                                                                                                                                                                                               gcacacctgattcctctctaaataccctagccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAAt                                            <  2:162291‑M2/217‑1 (MQ=255)                                                                                                                                                                                                  cacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGc                                         >  1:4717‑M2/32‑251 (MQ=255)                                                                                                                                                                                                   acctgattcctctctaaataccctatcagaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAACGATTCCTCAGTGGTCGCACACGCATCAATATTACTAAAAGGCAGTCTTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATACATACAGGGGGCAACTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGAGTTCATGTCCTCAAAATGGCg                                        >  2:273457‑M2/31‑251 (MQ=255)                                                                                                                                                                                                       gattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAAc                                    >  1:234552‑M2/27‑251 (MQ=255)                                                                                                                                                                                                                  aaataccctatccgaGTTGCGCTCCTCTTA‑CAAAAACGGACGTGAATTGTAAAGATTCCTCCGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAGGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCt                          >  2:314073‑M2/16‑250 (MQ=255)                                                                                                                                                                                                                    ttaccctatacgaGTTACGCTCCTCTTA‑AACAAACGGACGTGAATTGTAAAGATTCCTCAGCGGTCTATCCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATACGATACTTATTTTTTGAGTGCAATCCATACAGCGGACAAATCAAAAAAAGCCTATATTTCACTTTGCCCGCGCCGCGCAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGATAc                        >  2:128307‑M2/14‑250 (MQ=255)                                                                                                                                                                                                                              cgaGTAGCGCTCCTCTTA‑AAAAAACGTACGTGAATTGTAAAGATTGCTCAGTGGTCGCTCCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTGTTTTAGTGAAAGCCGTACAGCGGGCAAATCAAAAAAAGTCTATACTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCGATGCTACATTTTTTTGCt             <  2:67440‑M2/248‑1 (MQ=255)                                                                                                                                                                                                                               gaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCCGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAATAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGAAACGTCCTATGCTACATTTGTTTGCTg            >  2:237075‑M2/3‑251 (MQ=255)                                                                                                                                                                                                                                aGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCCGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTgg           >  2:435578‑M2/2‑251 (MQ=255)                                                                                                                                                                                                                                    gcgcTCATCTTA‑AAAAAACGGACGTTAATTGTAAAGATTCCTCAGTGGTAGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCTAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTg       <  2:505401/251‑1 (MQ=255)                                                                                                                                                                                                                                         cctcTTA‑AAAAAACGGACGTGAATTGTAAAGCGTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGGCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGGCCGCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGAttt  <  2:255068/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     GCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTT  >  NC_000913/3637187‑3637670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑