Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,795	G→A	Q128* (CAG→TAG)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,795	0	G	A	100.0%	51.6 / NA	16	Q128* (CAG→TAG)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/2);  total (14/2)

GGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑GTGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGC  >  NC_000913/2481572‑2482029                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            ggTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCAt                                                                                                                                                                                                                 >  1:88684/1‑251 (MQ=255)        tctcAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAAcccc                                                                                                                                                                                                          >  2:101972/1‑251 (MQ=255)                          taagtaagGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATAACCAACCCCAGCTAAAGTTATCACAAATCTAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTATTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TAAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAat                                                                                                                                                                                        >  2:437656/1‑251 (MQ=255)                                                         aaTCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATAAAGATTGGTCGATAGGCTac                                                                                                                                                         >  2:417355/1‑251 (MQ=255)                                                                    gaaaaTTGATAAAAAATACCCACCCCCAGAAAAATTAATCAAAAATAAAACCCCCCAAT‑GCGGCCCCACATATCGGAGAAATAACCCCGGTCATTGACCATAA‑TGCCAAAGAAAATTTTTTTTTTTCGGGCGGATAATCCCTTAAAAAAAACCTCT‑ATGACAGTGAAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa                                                                                                                                              <  1:416516/247‑1 (MQ=255)                                                                      aaaTTGATTAAAAATATCGAACACAAGGTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑AGCAAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATATTAAACTCT‑ATGACAGTGGAA‑TTAACGACACAGACATTAACCCCTGAACGACTCTAAAAACTATCAGCCCATCAAGATTGCTCGATAGGCAACACATTAATGaaa                                                                                                                                             >  2:333889/3‑249 (MQ=255)                                                                      aCATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGa                                                                                                                                               >  2:43747/1‑248 (MQ=255)                                                                                                             caAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACTGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                                                                                                      >  1:245344/1‑250 (MQ=255)                                                                                                                   taACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGcc                                                                                               >  1:176129/1‑251 (MQ=255)                                                                                                                           caaaagcGGCCC‑ACATGTGGGAGCGATAATGAGGGACATTGAC‑ATAAATGCAAGAGAAAATTTTTTTTTTTCGGGCGGATAATTCATTAA‑AAAAAAATCTTATG‑CAGTGGAAATAAACGCCCCCGCCATTAACCCCTGAACGACGCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCttt                                                                                       <  2:401569/245‑1 (MQ=255)                                                                                                                                           tatCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                                                          >  1:233971/1‑249 (MQ=255)                                                                                                                                                  gCGATAATCACGGGCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATCAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCATGAACGACTCTAAAAAATATCAGCACATCCAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGCCAAAAAAGTGACTGAAAGGAAAAATAATCTTAATTCGCCTATTCTTTGAGCCAACCTGCCAGTAAAAgg                                                                 >  2:9933/1‑251 (MQ=255)                                                                                                                                                   cGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTACTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGCCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGCAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAggg                                                                >  1:47986/1‑251 (MQ=255)                                                                                                                                                                                    gCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAggg                                >  1:371236/1‑250 (MQ=255)                                                                                                                                                                                                                  cTTAATAATAAACTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGc  >  1:375806/1‑251 (MQ=255)                                                                                                                                                                                                                            aaCTCT‑ATGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCtt                                                                                                       >  1:220363/1‑140 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            GGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCC‑ACATATCGGAGCGATAATCACGGTCATTGACCATAA‑TGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCT‑GTGACAGTGGAA‑TTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGC  >  NC_000913/2481572‑2482029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑