Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,177	A→G	intergenic (‑116/+257)	rfbB ← / ← galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,177	0	A	G	100.0%	122.8 / NA	34	intergenic (‑116/+257)	rfbB/galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

GTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGG  >  NC_000913/2112935‑2113422                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ggacattacatactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGcc                                                                                                                                                                                                                                               >  2:283960‑M2/133‑251 (MQ=255)            actcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATGAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGa                                                                                                                                                                                                                                     <  1:149659‑M2/129‑1 (MQ=255)                  gtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCGGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGt                                                                                                                                                                                                                              <  2:61270‑M2/136‑1 (MQ=255)                           gatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCGATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACt                                                                                                                                                                                                                      <  2:425197‑M2/144‑1 (MQ=255)                            atcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTcg                                                                                                                                                                                                                    >  1:395474‑M2/106‑251 (MQ=255)                                 cgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCt                                                                                                                                                                                                                >  2:401761‑M2/101‑250 (MQ=255)                                 cgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTa                                                                                                                                                                                                               <  2:355293‑M2/151‑1 (MQ=255)                                         atgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGa                                                                                                                                                                                                        >  1:280690‑M2/93‑250 (MQ=255)                                               gcggcctgaaatgttctcaaataagtatacaaagtacttattaaagtagaaatagcttacccatgcttatatgcttacggctttatTTATGCCATATTCCTTCAGAGCTTGCTTTGAAATAAGCGGCTCTCAGTTTGATAAATAGAGTTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATTCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTc                                                                                                                                                                                                 <  2:342339‑M2/165‑1 (MQ=255)                                                              cgcaaataagtagacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTc                                                                                                                                                                                   <  1:447116‑M2/179‑1 (MQ=255)                                                                         atacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGc                                                                                                                                                                       >  1:412377‑M2/61‑251 (MQ=255)                                                                            caaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTACTTGTCTTACCAATCCGctct                                                                                                                                                                    >  1:459353‑M2/58‑251 (MQ=255)                                                                              aagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTgg                                                                                                                                                                  >  1:234308‑M2/56‑251 (MQ=255)                                                                                  acttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGaaa                                                                                                                                                               <  1:227086‑M2/199‑1 (MQ=255)                                                                                     tattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGTGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACaa                                                                                                                                                            <  2:16125‑M2/202‑1 (MQ=255)                                                                                     tattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGATACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACa                                                                                                                                                             >  1:219437‑M2/49‑249 (MQ=255)                                                                                                 aatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGa                                                                                                                                               <  2:211770‑M2/215‑1 (MQ=255)                                                                                                         atccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTg                                                                                                                                       >  1:367440‑M2/29‑251 (MQ=255)                                                                                                                      tgcttacggctttatTTATTCCATATTACTTCAGAGAATGCTGTGAAATAAGCGGCTCTCAATTTGATAAATAGAGAGATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAAAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGCCTTACCAATCCGCTCTGGAAACAAGAAAAATCCTGGAAAACTTTGAATAAAACACTAc                                                                                                                          >  1:178963‑M2/16‑251 (MQ=255)                                                                                                                            cggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTa                                                                                                                     >  2:58530‑M2/10‑250 (MQ=255)                                                                                                                             ggctttatTTATTCCATATTACTTCAGCGCATGCTGTGAAATAAGCGGCTCTCAGTTTGCTTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAAc                                                                                                                   >  2:385935‑M2/9‑251 (MQ=255)                                                                                                                                     ttATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTTCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCTCTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCGCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCgttgt                                                                                                            <  1:376276/250‑1 (MQ=255)                                                                                                                                               ttACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATgg                                                                                                 >  1:7082/1‑251 (MQ=255)                                                                                                                                                tACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGt                                                                                                >  1:76749/1‑251 (MQ=255)                                                                                                                                                                                 cAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGcaac                                                                <  2:221366/250‑1 (MQ=255)                                                                                                                                                                                      tGATTAATAGGGGGATTAATGCCCGCTTCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGATCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAAtt                                                           <  2:234308/250‑1 (MQ=255)                                                                                                                                                                                       gATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTAc                                                         >  1:73521/1‑251 (MQ=255)                                                                                                                                                                                           aaTAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACttt                                                      <  2:7082/250‑1 (MQ=255)                                                                                                                                                                                                       aaTGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAAtttt                                         <  2:236393/251‑1 (MQ=255)                                                                                                                                                                                                                tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTGGCACTGcc                                  <  1:407700/249‑1 (MQ=255)                                                                                                                                                                                                                 aCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGAAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCg                                 <  2:11476/249‑1 (MQ=255)                                                                                                                                                                                                                                aCAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCa                <  1:256569/251‑1 (MQ=255)                                                                                                                                                                                                                                          gagCACTGCGTGCATGTCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACGCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACAt      <  1:385935/251‑1 (MQ=255)                                                                                                                                                                                                                                              aCTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCgg  >  1:436251/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCGG  >  NC_000913/2112935‑2113422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑