Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	121.4 / NA	34	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652087‑1652574                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aTGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                                                <  1:373757/251‑1 (MQ=255)   ggTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACCTTTACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                                                >  1:457319/1‑249 (MQ=255)    gtgtgtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAAACGACGCTCTCCGGAGTCCGATGACATTAAACAGACTGAGCAAAGAG‑CATATCATTTATGTTCACCTCCGAGAGt                                                                                                                                                                                                                                             >  1:17283/1‑251 (MQ=255)                ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAG‑CATATCATTGATGTTCACCTCCGAGAGTATGGcagaaca                                                                                                                                                                                                                                  >  2:151234/1‑250 (MQ=255)                       ggAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaaaa                                                                                                                                                                                                                            <  1:393778/249‑1 (MQ=255)                       ggAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGACATTCATCTTTTTAAGCCAGCAATCGTCGCTCCCCGGAGGCAGATGACATTAACGAGACTGAGCCAAAAG‑CATATCATTGATGTTCACCTCAGAAAGTATGGCCGAAAAAAAAAAc                                                                                                                                                                                                                           >  2:318745/1‑250 (MQ=255)                              gCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGAACGTTTCACGGTACCAAAAACACAGGCGGGGAACAAACGGGTCATTTATCCTATTAAGGCAGCAATCGACGCTATCCGGGGTCAGATGACATTAACGAGACTGAGAACAGAG‑CATATCATTGATGTTTACCTCAGATAGTATGGCAGAACAGAAAAAAAAACATg                                                                                                                                                                                                                    >  1:362841/1‑250 (MQ=255)                                               gCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAAc                                                                                                                                                                                                   <  1:402480/250‑1 (MQ=255)                                                cACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCt                                                                                                                                                                                                 <  1:271748/251‑1 (MQ=255)                                                    tGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGaa                                                                                                                                                                                              <  1:318745/250‑1 (MQ=255)                                                      gATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGACCGCAATCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAGCAAAAAG‑CATATCCTTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAgtg                                                                                                                                                                                           >  1:449150/1‑251 (MQ=255)                                                           gaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACTGTACCAAAAACACAGGCGGGCACCCATCGGGGCATTCCTCTTTTTAAGCCAGCCAACGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGAGTCAGc                                                                                                                                                                                      >  1:322995/1‑251 (MQ=255)                                                                                    cAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAt                                                                                                                                                             <  1:208779/251‑1 (MQ=255)                                                                                             tCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTGATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACCTTAACGAGACTGAGCAAAGAG‑CATATCATTGGTGTTCACCTCAGAGAGTATGGCAGAACAGCAAAACAAAAATGCACCTTTGTTTTTCAAACTGAAATGGCAGCGAGAGGAAAAAATTTTGGTGACCATTTT‑ACCGtt                                                                                                                                                   >  1:437890/1‑251 (MQ=255)                                                                                                    aaaTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAAAGAGACTGGGCAAAGAG‑CATATCATTTATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAAAGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTTACTCa                                                                                                                                             >  2:252289/1‑250 (MQ=255)                                                                                                      aTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAAACGACGCTCTCCGGAGTCCGATGAAATTAACGAGGCTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGGGACCATTTTTACC‑TTGACTCAAt                                                                                                                                           >  2:308449/1‑250 (MQ=255)                                                                                                           gCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTTTTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttttt                                                                                                                                                                                                           >  2:430501/1‑180 (MQ=255)                                                                                                           gCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGt                                                                                                                                                                                                           <  1:430501/182‑1 (MQ=255)                                                                                                               aaGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGGCCTTCCTCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACCAGACTGAGGAAA‑AGACATATTATTTTTGTGCACCTCAGAGAGGATGGCAGAAAAAGAAAACAAAAATGCAGCTTTTTTTTTCAACCTGTAATGACAGCGGGGGAAAAAAATTTTGGGGATCATTTT‑ACCCTTGACTCAATAAGGCCGAtg                                                                                                                                 >  1:171803/1‑251 (MQ=255)                                                                                                                         cACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCa                                                                                                                        <  2:438706/250‑1 (MQ=255)                                                                                                                            ggTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCg                                                                                                                     >  1:185282/1‑250 (MQ=255)                                                                                                                                                  aCCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCa                                                                                              <  1:151234/251‑1 (MQ=255)                                                                                                                                                                               aGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACtt                                                                 <  1:451746/251‑1 (MQ=255)                                                                                                                                                                                      gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctg                                                          >  1:424281/1‑251 (MQ=255)                                                                                                                                                                                             tcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGCCGAGAAATACTTATGCCTGCTGGTCg                                                   >  2:221015/1‑251 (MQ=255)                                                                                                                                                                                              ccGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGt                                                     <  2:63655/248‑1 (MQ=255)                                                                                                                                                                                                             aTTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCTCCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCGTGTTTTTTCAACCGGAAATGTCAGCGAGAGTAAAAAATTATGGTGGCCATTTT‑ACCGTTGACTCAATAAGGCAGATGTAGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTa                                   <  2:270888/251‑1 (MQ=255)                                                                                                                                                                                                              ttAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTa                                   >  2:382541/1‑250 (MQ=255)                                                                                                                                                                                                                aaCGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAGGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAGCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAAc                                 <  2:17283/250‑1 (MQ=255)                                                                                                                                                                                                                   gagaCTGAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCgg                             >  1:199643/1‑251 (MQ=255)                                                                                                                                                                                                                         gAGCAAAGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTta                       <  1:309518/251‑1 (MQ=255)                                                                                                                                                                                                                          aGCAAAGAG‑CAAATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGAGCTAACCCGGCATTtat                      >  2:159821/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaGAG‑CATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGATGGTGCTAACCCGGCATTTATAGc                   >  1:103392/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATg  <  2:457319/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAG‑CATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT‑ACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652087‑1652574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑