Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,978	C→A	intergenic (‑103/‑6)	mngR ← / → mngA	DNA‑binding transcriptional repressor MngR/2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,978	0	C	A	100.0%	191.6 / NA	49	intergenic (‑103/‑6)	mngR/mngA	DNA‑binding transcriptional repressor MngR/2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/31);  total (18/31)

CGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATCACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGCC  >  NC_000913/765741‑766203                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                  cGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTAtt                                                                                                                                                                                                                      <  1:268136/251‑1 (MQ=255)  gTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTg                                                                                                                                                                                                                     >  2:339996/1‑251 (MQ=255)    aaCCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGtt                                                                                                                                                                                                                   <  1:307435/251‑1 (MQ=255)     aCCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGttt                                                                                                                                                                                                                  <  2:75046/251‑1 (MQ=255)      cccGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGTCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCACGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTTATAACATAATGGTATTGtttt                                                                                                                                                                                                                 <  2:77934/251‑1 (MQ=255)           cTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCgg                                                                                                                                                                                                            >  1:231162/1‑251 (MQ=255)             gACGCCAAACTCTGTTTGTGTCGCTGATTCAGTGGGTAACGCATCGCCAGTTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCggg                                                                                                                                                                                                           <  2:14412/250‑1 (MQ=255)                   aaaatctGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTg                                                                                                                                                                                                    <  2:207738/247‑1 (MQ=255)                   aaaCTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTg                                                                                                                                                                                                    >  2:53219/1‑251 (MQ=255)                   aaaCTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTg                                                                                                                                                                                                    >  2:112360/1‑251 (MQ=255)                          gTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGcgcg                                                                                                                                                                                               <  1:417651/249‑1 (MQ=255)                              gTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACtat                                                                                                                                                                                          >  1:38005/1‑250 (MQ=255)                                  cgcTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATaaaaa                                                                                                                                                                                     <  2:308242/251‑1 (MQ=255)                                          cAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAg                                                                                                                                                                             <  2:131576/251‑1 (MQ=255)                                                      cATCGCCAGGTTTCAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCatg                                                                                                                                                                  >  2:453680/1‑250 (MQ=255)                                                       aTCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCatgat                                                                                                                                                                >  2:190776/1‑251 (MQ=255)                                                              gttttAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCACGATAAATTCATCCTGTAAATAATACAAATAAAATACAAATAATTTCAATCAAAAGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGAACAAGTGAGGATAATGAGGAATCTg                                                                                                                                                         >  2:246642/3‑251 (MQ=255)                                                                  tAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTgacg                                                                                                                                                      >  2:258562/1‑250 (MQ=255)                                                                     gCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGActc                                                                                                                                                  >  2:156574/1‑251 (MQ=255)                                                                         gCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCAGTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGAGGAATCTGACGACTCTGAc                                                                                                                                              <  2:173032/251‑1 (MQ=255)                                                                              gcgcAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGCCTCTGACCCAcc                                                                                                                                         >  2:198695/1‑251 (MQ=255)                                                                                      tGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGc                                                                                                                                  <  1:258562/250‑1 (MQ=255)                                                                                         tCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGAGGCGtt                                                                                                                               <  1:453680/250‑1 (MQ=255)                                                                                            cGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTgtgt                                                                                                                           <  2:455315/251‑1 (MQ=255)                                                                                                  cGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAAt                                                                                                                     <  1:112360/251‑1 (MQ=255)                                                                                                     tCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATgcg                                                                                                                  >  1:297908/1‑251 (MQ=255)                                                                                                               gCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTAcc                                                                                                         >  1:63218/1‑250 (MQ=255)                                                                                                                  ggTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCGGAATGCGCGCTTTACCAGcc                                                                                                     <  2:38005/251‑1 (MQ=255)                                                                                                                    tttaAGGGCTTGTGTCCCATTTGTTTTTTCTCTTTTCTACGAATTTCACCATTATGCCCGATAAATTCATCCTGTAAATATTACAAATTCAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGTGTTGTGTCTGAATGCGCGCTTTACCAGct                                                                                                     <  2:96758/247‑2 (MQ=255)                                                                                                                              tgtgTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGcc                                                                                          <  1:393801/250‑1 (MQ=255)                                                                                                                               gtgtCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCAt                                                                                        <  1:259949/251‑1 (MQ=255)                                                                                                                                     cATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCAcgc                                                                                  <  2:158641/251‑1 (MQ=255)                                                                                                                                         tttAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGtt                                                                               <  1:156574/250‑1 (MQ=255)                                                                                                                                                    aTTAATACAAATTTAACCATTATGCCCTATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTAGCGGGCGCACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGAGGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCt                                                                   <  2:439117/251‑1 (MQ=255)                                                                                                                                                      tAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCTCACTGGCGCGTCTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGACGCCTTGTTTTTGAATCCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGGTGACTCAACGTCtt                                                                  <  2:129041/250‑1 (MQ=255)                                                                                                                                                          aCGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTtgctg                                                              <  1:232286/250‑1 (MQ=255)                                                                                                                                                                ttAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTgg                                                        <  1:197123/250‑1 (MQ=255)                                                                                                                                                                   aCCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGaa                                                    <  1:425660/251‑1 (MQ=255)                                                                                                                                                                        tATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAAtt                                                <  1:286260/250‑1 (MQ=255)                                                                                                                                                                                               aaaTAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGga                         >  2:26995/1‑250 (MQ=255)                                                                                                                                                                                                aataatACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGgaag                       <  1:40224/251‑1 (MQ=255)                                                                                                                                                                                                    atacaaatacaaTACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAgt                   >  1:264021/1‑251 (MQ=255)                                                                                                                                                                                                      acaaatacaaTACAAATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAgtgt                 >  1:338507/1‑251 (MQ=255)                                                                                                                                                                                                             caatacaaATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTtgct                                                               >  1:48389/1‑198 (MQ=255)                                                                                                                                                                                                             caatacaaATAATTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGGCTtgct                                                               <  2:48389/198‑1 (MQ=255)                                                                                                                                                                                                                     ataatTTCAATCAAGTGAAATTTATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGAATATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGcc  <  2:272878/251‑1 (MQ=255)                                                                                                                                                                                                                     ataatTTCAATCAAGTGAAATTGATAACATAATGGTATTGTTTTAACGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGcc  <  2:209092/251‑1 (MQ=255)                                                                                                                                                                                                                                caaGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGAc                                                                            <  2:116793/166‑1 (MQ=255)                                                                                                                                                                                                                                caaGTGAAATTGATAACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGAc                                                                            >  1:116793/1‑166 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                  CGTAACCCGGCTGACGCCAAACTCTGTTTGTAGCGCTGATTCAGTGGGTAACGCATCGCCAGGTTTAAGCTCGCCACGCGCAATTTGTTCACGAATGCGATCGGCAATCTGCCGGTATAAGGGCTTGTGTCCCATTTTTAGTATCTCATTAATACGAATTTAACCATTATGCCCGATAAATTCATCCTGTAAATAATACAAATACAATACAAATAATTTCAATCAAGTGAAATTGATCACATAATGGTATTGTTTTATCGGGCACACTGGCGCGACTATAAAAACGATCAAGTGAGGATCATGATGAATCTGACGACTCTGACCCACCGCGATGCGTTGTGTCTGAATGCGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGCC  >  NC_000913/765741‑766203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑