Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	97.4%	134.3 / ‑3.6	38	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (15/22);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

GTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTT  >  NC_000913/674276‑674761                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gttcgttcGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTCCGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTcc                                                                                                                                                                                                                                             <  1:464457/251‑1 (MQ=255)           aagttcGGTCTGTTCGCTCGGGCACCGGTTGACCGCAGAAGTCTTCTTATATCCCAGGCCTTTTTTATACAGCTCGGAGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGCCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATTTTGTCGTCCGTCCCCGGTGCCGGGGCGGTTTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGAc                                                                                                                                                                                                                                   <  2:197039/246‑1 (MQ=255)             gTACGGTCTGGTCGGTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCTTTTTTTAAACAGCTCGGTGAAGAATTCCTGTTCCCAGCGGTAGGATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCGGGTTTTTCATATACGCGATGTTGTCGTCCGTCCACGGTGCCGGAGGGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCa                                                                                                                                                                                                                                 <  2:103330/251‑1 (MQ=255)               aCGGGCTGGTCGTTCGGGCAACAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGGGCTGGTTTTTAATATACGCGTTGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGCCCaa                                                                                                                                                                                                                                >  2:237771/1‑250 (MQ=255)                cGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATCCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAc                                                                                                                                                                                                                              <  1:104502/251‑1 (MQ=255)                      ggTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTcc                                                                                                                                                                                                                        <  1:324150/251‑1 (MQ=255)                          gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCACACGCGTCCCAGc                                                                                                                                                                                                                    >  2:227001/1‑251 (MQ=255)                                                          tatataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCCCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCa                                                                                                                                                                                      <  1:376935/249‑1 (MQ=255)                                                              taCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCt                                                                                                                                                                                >  1:316431/1‑251 (MQ=255)                                                                             ataCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                                                                                                                  >  2:317724/1‑250 (MQ=255)                                                                                    tCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                                                                                                                          >  1:145250/1‑251 (MQ=255)                                                                                     gggTGAAAAATGTCTGTTCCCAACGGTATTTTCCCGGCGTCCAGGTTGCCAGCCCGCTGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTGGTCGTACGTCCGCGGTGCCGGAGCGGTGTTGTTTTTCACCGCAACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                         <  1:397093/250‑1 (MQ=255)                                                                                          aagaaTTTCTGTTCCCAACGGTATTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATACCCAAA‑GCCCAGCTTTTTGAGCTGTTTTTTCATTTCCGCTATGTTGTCGTACGTCCCCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgt                                                                                                                                                    <  1:334641/251‑1 (MQ=255)                                                                                            gaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgtgt                                                                                                                                                  >  1:467668/1‑251 (MQ=255)                                                                                                      ccccAACGGGAGTATGACGGCGTACAGTTGGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGGGAGGTTGTCGTACTTCCACGGTGCCGGAGGGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtac                                                                                                                                        <  1:237771/250‑1 (MQ=255)                                                                                                           aCGGTAGTATTCCGGCGTACGGGTTGCCAGCTCGCGGCTCAAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGGGGGGTTTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGc                                                                                                                                   <  1:116921/251‑1 (MQ=255)                                                                                                             ggTATTATTCCGGGGTACAGGTTGCCTGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGTGATGTTGTCGTCCGTCCCCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAGCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTTCGTGGccc                                                                                                                                 <  1:332029/251‑1 (MQ=255)                                                                                                                          cGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                                                                                                                    >  1:87916/1‑251 (MQ=255)                                                                                                                          cGTACAGGTGCCCAGCTCGCGGCTCCGGTCATAACCAAA‑GCCCAGCATTTGGAGCTGTTTTTTCATATACGCGTGGTTGTCGTACGTCCACGGTGCCGGGGCGGTTTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                                                                                                                    <  1:360880/251‑1 (MQ=255)                                                                                                                           gTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                                                                                                                   >  1:324582/1‑251 (MQ=255)                                                                                                                                             gggcaccgttCATAACCAAG‑CCCCAGCTTTTGGAGCTGGTTTTTCATATCCGGAATGTTGTCGGCCGCCCCGGGTGCCGGAGCGGTTTTGTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                                 <  2:61607/242‑1 (MQ=255)                                                                                                                                               gCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                                 >  1:247233/1‑249 (MQ=255)                                                                                                                                               gCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTCCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                                 >  2:246535/1‑249 (MQ=255)                                                                                                                                                          aagccaagccccggCATTTGGAGCTGGTTTTTAATATACGCGATGTGGTCGTCCGCCCACGGTGCCGGGGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTa                                                                                     <  2:145250/238‑1 (MQ=255)                                                                                                                                                           aCCAAACGCCCAGACTTTTGGGCTGGTTTTTCATATCCGCGTTGTTGCCGGCCGTCCCGGGTGCGGGAGGGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTaa                                                                                    <  2:155312/251‑1 (MQ=255)                                                                                                                                                           aCCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAAt                                                                                   <  1:227001/251‑1 (MQ=255)                                                                                                                                                              aaa‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACt                                                                                <  1:420271/251‑1 (MQ=255)                                                                                                                                                                      aGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCttt                                                                         >  1:423357/1‑251 (MQ=255)                                                                                                                                                                         aTTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGct                                                                      >  2:217708/1‑251 (MQ=255)                                                                                                                                                                          ttttGAGCTGGTTTTTCATATCCGCGATTTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGct                                                                      <  1:105044/250‑1 (MQ=255)                                                                                                                                                                               aGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCAGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAgg                                                                                                                                                                                                      >  1:98600/1‑117 (MQ=255)                                                                                                                                                                               aGCTGGTTTTTCATATACGCGATGTTGTCGTACGCCCCCGGTGCCAGAGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAgg                                                                                                                                                                                                      <  2:98600/117‑1 (MQ=255)                                                                                                                                                                                         tCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACt                                                      >  2:340656/1‑251 (MQ=255)                                                                                                                                                                                                   gttgttctCGTCCGCCCAGGGTGCCGGAGCGGTGTTTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgc                                            <  2:247233/249‑1 (MQ=255)                                                                                                                                                                                                    gggtggtcgtccgccccgggggCCGGGGCGGTGTTTTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgcg                                           <  2:269510/230‑1 (MQ=255)                                                                                                                                                                                                       ttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCtt                                        >  2:269225/1‑251 (MQ=255)                                                                                                                                                                                                                              aGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                 >  1:248782/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtct     <  1:423671/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCtt  <  2:467668/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAA‑GCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTT  >  NC_000913/674276‑674761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑