Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,518,356	A→G	intergenic (‑121/‑16)	fecI ← / → ykiE	RNA polymerase sigma factor FecI/protein YkiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,356	0	A	G	100.0%	171.1 / NA	47	intergenic (‑121/‑16)	fecI/ykiE	RNA polymerase sigma factor FecI/protein YkiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/16);  total (31/16)

CATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTATAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTCGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTG  >  NC_000913/4518118‑4518599                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    cATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATCTGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGGTTTTCTAAGTGTTGTAAGGTAGGtat                                                                                                                                                                                                                                         >  1:278765/1‑251 (MQ=255)      gcctCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATaaa                                                                                                                                                                                                                                      <  2:196137/246‑1 (MQ=255)      gCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAAt                                                                                                                                                                                                                                    >  1:245704/1‑251 (MQ=255)       cATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTACGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTTGAAATAAAATTTTTTCCAATAGACATTTTTAACATGTTGATTTTCTAAGTGTTGTACGGTAGGTATaaactg                                                                                                                                                                                                                                   >  2:418539/1‑248 (MQ=255)            aaaGCAGACTGGAGTTTGCGCGTCAGCGAGCTTTTCAACCAGACGTGATGAGTGACATAAAGCGACTAGAACGTTAAGGAAGCTGTGGTAGTGGCGAGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCAGGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTTCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATg                                                                                                                                                                                                                              >  2:66953/1‑251 (MQ=255)                cAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGc                                                                                                                                                                                                                          >  1:29165/1‑251 (MQ=255)                     tGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTg                                                                                                                                                                                                                     >  1:209085/1‑251 (MQ=255)                          tttGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTTGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGTTTATCATTACAATTGGAAATAAAATTGTTGCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTATGTATAAAATGTGATGGAGCCTCTGCTTc                                                                                                                                                                                                                 <  1:146170/250‑1 (MQ=255)                                 gTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTTAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAAAAGACATTTTTAACATGTTGTTTTTCTAAGGGTTGAAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCAttt                                                                                                                                                                                                         >  2:439803/1‑249 (MQ=255)                                     gccagcTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCg                                                                                                                                                                                                     >  2:163299/1‑251 (MQ=255)                                        agcTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTc                                                                                                                                                                                                  <  2:403451/251‑1 (MQ=255)                                              gcAACCAGCCGTGATGTGGGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAAt                                                                                                                                                                                             <  2:204314/249‑1 (MQ=255)                                                  acAGCCGTGATGTGGGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGAGGCGCGGTCAGACATGCGGAGGGCATCAAAAGTTAATTATCAAGTAGTCAAATTAATATGAGAATGGTTATCATTACAATTGGACATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTATAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTTTGCTTCTGTCAtgttgcggttcgaaagactc                                                                                                                                                                                        >  2:23965/2‑234 (MQ=255)                                                          gATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGAtgt                                                                                                                                                                                 <  2:69551/250‑1 (MQ=255)                                                               gtgCCATAAAGCGACGCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCt                                                                                                                                                                            <  2:245704/250‑1 (MQ=255)                                                                      aaaGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActca                                                                                                                                                                    <  2:418769‑M1/251‑5 (MQ=255)                                                                                gAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaatt                                                                                                                                                          >  1:67525‑M1/1‑237 (MQ=255)                                                                                  aCGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgt                                                                                                                                                        <  2:29165‑M1/251‑17 (MQ=255)                                                                                         ggAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgc                                                                                                                                                   >  2:410152‑M1/1‑228 (MQ=255)                                                                                           aaGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgt                                                                                                                                                      >  1:234940‑M1/1‑226 (MQ=255)                                                                                           aaGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgt                                                                                                                                                      <  2:234940‑M1/244‑19 (MQ=255)                                                                                                    tAGTGGCGCGGTCAGACATGCGGCGTGCATCACAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGAATCATGATGTGGTCTGCTActcaaggtggcattgtgtcgcagtataccgcc                                                                                                                                        >  2:414435‑M1/1‑217 (MQ=255)                                                                                                         gcgcGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacg                                                                                                                                 <  1:418851‑M1/251‑40 (MQ=255)                                                                                                                cAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggatt                                                                                                                           >  2:295284‑M1/1‑205 (MQ=255)                                                                                                                  gACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtttcgcagtataccgccagaaacggggattaat                                                                                                                        <  1:173527‑M1/251‑49 (MQ=255)                                                                                                                        cGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctt                                                                                                                  <  1:261989‑M1/251‑55 (MQ=255)                                                                                                                         agaGTGAATCAACAGTTAATTATCACGTCGTCATATTACCATGAGAATGGTTATCATTACAATAGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGCAGGTATAAACTGGACTGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtgaaattgtgtcgcagtataccgccagatacggggattaatactcttt                                                                                                                 >  2:398861‑M1/2‑196 (MQ=255)                                                                                                                                  cAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttac                                                                                                        >  2:376260‑M1/1‑187 (MQ=255)                                                                                                                                     aaGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccg                                                                                                     >  1:436161‑M1/1‑184 (MQ=255)                                                                                                                                                   gTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctat                                                                                       <  1:431012‑M1/251‑82 (MQ=255)                                                                                                                                                     aGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcc                                                                                     >  1:22075‑M1/1‑168 (MQ=255)                                                                                                                                                         atatTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagt                                                                                 >  2:417639‑M1/1‑164 (MQ=255)                                                                                                                                                                tatGAGAATGGTTATCATTTCAATTGGAAATAAAATTGTTTCCAATCGACATTTTTACCTTGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAATTGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGCGGTCTGCTCctcacggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttccccggctacggcagctatcccagtttaagct                                                                          >  2:122080‑M1/1‑157 (MQ=255)                                                                                                                                                                 atGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagat                                                                          <  1:453915‑M1/250‑95 (MQ=255)                                                                                                                                                                   gagaATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGAAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcac                                                                       >  2:356109‑M1/1‑154 (MQ=255)                                                                                                                                                                    agaATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacc                                                                      >  2:129915‑M1/1‑153 (MQ=255)                                                                                                                                                                      aaTGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcaccc                                                                     >  1:46936‑M1/1‑151 (MQ=255)                                                                                                                                                                              tCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaataatctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgt                                                            >  2:96058‑M1/1‑143 (MQ=255)                                                                                                                                                                                                        tCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcac                                   <  2:22075‑M1/250‑134 (MQ=255)                                                                                                                                                                                                               gACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgc                           >  2:90374‑M1/1‑110 (MQ=255)                                                                                                                                                                                                                     tttAACATGATGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcacagttgaagataacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagc                      >  2:180426‑M1/1‑104 (MQ=255)                                                                                                                                                                                                                        aaCATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaact                  >  1:8224‑M1/1‑101 (MQ=255)                                                                                                                                                                                                                             gttgttTTTCTAAGTGAGGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcacagcaacttggtn             <  2:278765‑M1/251‑156 (MQ=255)                                                                                                                                                                                                                                 tttttCTAAGTGTTGTAAGGTAGGAATAAAATGGTCTGGCGCCTCTGCTTCTGGCATGTGTCGGTCAGTATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagaataccgccagaaacggggattaatacgctttacctgttacccggctacggtagctctccgagttaaagatcacccacaaactgttacccagtcgcatacggtccatcacgcgatcgcccagcaacttggtcatct         >  2:76330‑M1/1‑92 (MQ=255)                                                                                                                                                                                                                                   tttCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctct       >  1:133765‑M1/1‑90 (MQ=255)                                                                                                                                                                                                                                      cTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctcttcc    >  2:58916‑M1/1‑87 (MQ=255)                                                                                                                                                                                                                                         aGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctcttccat  >  2:323399‑M1/1‑84 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    CATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTATAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTCGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTG  >  NC_000913/4518118‑4518599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑