| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,434,861 | 0 | G | T | 24.6% | 111.7 / 13.9 | 61 | P259Q (CCA→CAA) | truA | tRNA pseudouridine(38‑40) synthase |
| Reads supporting (aligned to +/- strand): ref base G (13/33); new base T (15/0); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCGGTTTCACAGAACAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCG > NC_000913/2434615‑2435111 | gACCGGTTTCACAGAACAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGa > 1:214478/1‑249 (MQ=255)gACCGGTTTCACAGAACAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATGCTCGGTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAg < 2:423090/251‑1 (MQ=255) ccGGTTTCACAGAACAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATTTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGT‑TTTTGGAAGA‑t < 1:399174/251‑1 (MQ=255) ggTTTCAAAGAACAAAATTACGAACAAAAGGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTATGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGCCCATATTTGCCTATGCCTTGAAATCGTCATCTTTCAGGCTGTCACTACGTTGGCTACAGCCTGAAATGTTCCGAGACTTATTCTCGTTAGTCCGCCAGAAATAGCGGGCACATTGGCGGGTTTTggtgtca > 2:162190/1‑245 (MQ=255) acaGAACAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGTAGA‑T‑cctaaccg > 2:349403/1‑244 (MQ=255) aaCAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑T‑AATAcagggc > 1:193634/1‑244 (MQ=255) aaaTTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTTAAGA‑T‑CACACCGGTccaggt > 2:414611/1‑245 (MQ=255) aaGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGA‑T‑CATACCGGTCAGGGGAATccc > 1:99297/1‑250 (MQ=255) gAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGGA‑GA‑T‑CATaacactcagggggaaccacc > 2:128791/1‑232 (MQ=255) aaCAAAATGGCATAAACCCAGACGCCGTACTCCGCGTCCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑CATACCGGTCAGGGGAATCCAccc > 1:198014/1‑250 (MQ=255) aaaaTGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TGTTTGACGA‑T‑AATACCGGTAAGGGGAAaccaccacg > 1:273598/1‑247 (MQ=255) aTGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTTTTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGACT‑C‑TAACGGTCAGGGGAAaccaccccgcccc > 1:439748/1‑244 (MQ=255) cATAACCCCAGACGCCGGACGCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAAGCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATa < 2:318198/251‑1 (MQ=255) tAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATTCCCGTCAGGGGAATCCACCGCGACCAGATAcc > 1:328453/1‑250 (MQ=255) aGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑CATACCCGTCAGGGGAATCCACCGCGACCCGATACA‑Acccctc > 1:59748/1‑248 (MQ=255) gCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑ATTTTGAAGA‑G‑CATACCGGGGAGGGTAATCCACCGACACCAGAGACAAGCCCtccgcc > 1:130767/1‑246 (MQ=255) cTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGG‑T‑CATAACGGACAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGcc > 1:93629/1‑251 (MQ=255) gACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑TACA‑ACCGGGCCGGGGAATACCCCGCGACCACACACA‑ACCCTCCCGCTTTTGTCCTtgct > 2:29537/1‑251 (MQ=255) gACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCAGATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTtgct < 2:211293/251‑1 (MQ=255) aCCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTtgctg < 2:76313/251‑1 (MQ=255) cAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGA‑T‑CATACCGGGGAGGGGAATCCACCGCGACCAGATACA‑GCCCCTCCGCCTTTTCCGTTGCTgcc > 1:54596/1‑251 (MQ=255) ctGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTgccgcc < 2:272896/249‑1 (MQ=255) gATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCt < 2:11277/251‑1 (MQ=255) aTGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑CATACCGGGCAGGGGAATTCCCCCCGCCCCGATAAA‑CGCCTTCTCCTTTTTCCGTTGCTGCCCCCacgctcttt > 2:283429/1‑243 (MQ=255) gCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTc < 1:38860/251‑1 (MQ=255) cGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑CTTTGGGAGA‑T‑CATAACCGCCAGGGGAATCCACCCCCACCACATACA‑GCCCTTCCCCTTTTTCCCTTTCTGCCGCCacacgtctctcctt > 2:399855/1‑238 (MQ=255) gCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCa < 2:210574/250‑1 (MQ=255) gCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑ATTTGGAAGA‑T‑CATAACGGGCAGGGGAAACCACCGCGCCCCAATACA‑GCCCCTCCGCTTTTCCCCTTGCTGCCGCCCGCGGTCTGTCCCTTTgcgccac > 1:392070/1‑246 (MQ=255) aTAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑CATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTTCCCCTGCTGCCGCCCGCGTTCTGTCC‑CTTTCCGCCCGCaaac > 1:374326/1‑249 (MQ=255) atCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑AATACCCGGCAGGGGAATCCACCGCGACCAGATACA‑AACCCTCCCCTTTTGCCGTTGCTTCCCCCCGCGTTCTGTCC‑TTTTCCCCCAACAAATCTTCTATCCACCTCTCCGGCt > 1:455199/1‑251 (MQ=255) tatTTGCATATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑T‑CCTAACGGTCAGGGGAATCCACCCCGACCAGGAATA‑ACCCCTCCGCTTTTTCCGCTGGTGCCGCCAGCCTGCTTTCC‑TTTTCCGCCCGCGACGATGCTTTCCAACTCTCCCGCTgggggtgggg > 1:109861/1‑243 (MQ=255) tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAcc < 2:268902/250‑1 (MQ=255) gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCg < 2:113843/250‑1 (MQ=255) ccTATGCCTTGTGCTCGTCATCTTTCAGGCTGTAACTACGGTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGGCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGGTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc < 1:114386/251‑1 (MQ=255) ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCAt < 2:448005/251‑1 (MQ=255) aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTCCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGc < 2:264624/250‑1 (MQ=255) gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGA‑T‑CATACCCGGCAGGGTAAACCACCGCCACCCGATACA‑GCCCTTCCCCCTTTGCCCGTGCTGCCGCCCGCGGTCTGTCC‑TTTTGCGCCAGCCACACCGATATTCAGCTCCCCGGCCGGGTGTGGGCACCCGC‑TTCCATCCGGCTGccc > 2:186263/1‑250 (MQ=255) tgcAGGCTGTAACTACGTTGGCGACAACCTGAAAGGTGCCGAGCATAATACTAGATAGTCCGCCAGAAATGGCGGGCCCGTGGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCGCCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCGGGCTGCCGAccat < 2:198007/247‑4 (MQ=255) cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑CATACCGGTCAGGGGAATCCACCGCGACCCGATACA‑GCCCTTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCC‑TTTTCCGCCCGCAACTCTTCTATCCAGCTCTccc > 1:303250/1‑196 (MQ=255) cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg < 2:303250/197‑1 (MQ=255) aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATatg < 2:59748/250‑1 (MQ=255) gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑T‑CATAACCGTCAGGGGAATCCACCGCGACCAGATACA‑ACCCCTCCGCTTTTTCCGTTTCTGCCGCCCACGTTCTGTCC‑TTTGCCGCCCGCAAATCTGCTTTCCAGCTCTCCCGCTGGGTTTGGGCCCCCACCTTCC‑TCCGGCTGCCGACAATAATCCTCACCCTatgatg > 1:371137/1‑251 (MQ=255) gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATatgatg < 1:349403/251‑1 (MQ=255) aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACaaaa < 2:198014/251‑1 (MQ=255) aCAGCCTGAAATGTTCCGAGCATTATGCTCGTTAGTCCGCCATAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCAGCAGGCTGCCGACAATATTCCTGACCATATGATGTACaaaa < 2:87581/251‑1 (MQ=255) gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATCCCTGACCATATGATGTACAAAAGCa < 2:214478/251‑1 (MQ=255) tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGA‑A‑CAAACCGGTCAGGGTAATCCACCGCGACCCGATACA‑GCCCTTCCGCCTTTGCCGTTTCTGCCGCCCGCGTTCTTGCC‑TTTGCCGCCCGCCACTCTTCTATCCACCTCTCCCGGTGGGTGTGGGCACCCAC‑TTTCATCAGGCTGCCCACAAAATTTCTGACCATATGATGTACAAAAGCATTCGcctttat > 1:329280/1‑245 (MQ=255) tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCAGTCGCTTTaa < 2:371137/250‑1 (MQ=255) attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTacc < 1:391687/251‑1 (MQ=255) cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGA‑T‑CATACCGGTCAAGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCCCCAGCGTTCTGTCC‑TTTGCCGCCAGCAAATCTTCTTTCCAGCTCTCCCGCTGGTTGTGGGCACCGAC‑TTCCCTCAGGCTGCCGACAATATTCCTGGACATATGGTGTAAAAAAGCATTTGCTTTAATATCTACCACcacac > 1:267681/1‑249 (MQ=255) cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACcaca < 2:74995/249‑1 (MQ=255) ccGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAata < 2:214007/230‑1 (MQ=255) ccGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGGAATCCACCCCGACCAGATACA‑GCCCCTCCGCCTTTGCCGTTGCTGCCCCCCGCGTTCTGTCC‑TTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCACCGAC‑TTCCCTCCGGCTGCCGGCAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTtataagatctgcagagcacacggcc > 1:214007/1‑230 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGt < 1:310421/249‑1 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGa < 1:399855/251‑1 (MQ=255) aaGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCCCCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGAc < 2:328453/250‑1 (MQ=255) gAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGAcgc < 1:461443/251‑1 (MQ=255) aGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCATGGTAATCCACCGCGACCAGATACC‑GCCCTTCCGCTTTTGCCGTTTCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCTACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCGCCACATAAGGACCGTGACGCGTGAc < 1:182846/251‑1 (MQ=255) cATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCCCCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCTCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAatat < 2:99297/251‑1 (MQ=255) ttGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGc > 1:411172/1‑251 (MQ=255) tGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGc < 2:54596/250‑1 (MQ=255) ggCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCAt < 1:376054/251‑1 (MQ=255) ggAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTgcg > 1:157159/1‑251 (MQ=255) ggAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTgcg > 1:55766/1‑251 (MQ=255) | GACCGGTTTCACAGAACAAAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGA‑T‑CATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCC‑TTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAC‑TTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCG > NC_000913/2434615‑2435111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |