Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	433,641	0	G	A	20.3%	82.3 / 10.4	65	A63T (GCC→ACC)	ribD	fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase
Reads supporting (aligned to +/- strand):  ref base G (21/30);  new base A (13/0);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 7.98e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAC  >  NC_000913/433398‑433885                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTGTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGTTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAgg                                                                                                                                                                                                                                                <  2:275100/251‑1 (MQ=255)      cGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGGAAAAGCCAAAGGTGCg                                                                                                                                                                                                                                            >  1:447146/1‑250 (MQ=255)             atCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCt                                                                                                                                                                                                                                     <  2:51452/250‑1 (MQ=255)               cAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGGAAGGCGGGTGGAAAAACCaaaagaggcaccccccacg                                                                                                                                                                                                                                  >  2:43777/1‑235 (MQ=255)                   gAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGCCAAAAGTGGGACCCCCTATGTcac                                                                                                                                                                                                                              >  1:373009/1‑251 (MQ=255)                         agCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGGTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGGCAAAAGAGGGACCGGCTATGTcaccagcg                                                                                                                                                                                                                         >  2:75383/2‑245 (MQ=255)                            gAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGAGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTAACACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCAAAAGTGCggccacctctctcacccacgaaac                                                                                                                                                                                                                     >  2:256787/1‑228 (MQ=255)                               gagaTCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGAGCGACCGCCTATGTCACACTCGAAACCTg                                                                                                                                                                                                                  >  1:158458/1‑251 (MQ=255)                                agaTCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTAGAGCACAGAGGACGTTTTACCACGCATCCCAACCCGACTG‑TCGGGTGCGTCATTGTCAAAGCTGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACAACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGACAAAGGGTCGAACCCCCATGTCACACTCGAACCCTGt                                                                                                                                                                                                                 >  1:360828/1‑251 (MQ=255)                                     gcgcgcCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGTGCGACCGCCCATGTCCCACTCGGACCCTGTAGcc                                                                                                                                                                                                             >  1:334675/1‑250 (MQ=255)                                        ggcCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCCCGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatca                                                                                                                                                                                                         <  1:109983/250‑1 (MQ=255)                                         gcCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAAGTGCGACCGCCTATGTCACAATCGGACCCTGGAGCcatcat                                                                                                                                                                                                        >  1:445117/1‑251 (MQ=255)                                                     aaGCCGTGCAGGAGGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAGCCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGGGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                            <  1:130214/251‑1 (MQ=255)                                                     aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGACAAAAGGGCGGCCGCCTATGTCACACTCGAACCCCGTAGCCCTCATGGTCGGACGccc                                                                                                                                                                                            >  1:5722/1‑250 (MQ=255)                                                                       aTTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGGAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGGGCTGTGACGCACTc                                                                                                                                                                          >  1:46438/1‑251 (MQ=255)                                                                         tACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATAGTCGTACGCCACCGTGATGTGACGCACTCa                                                                                                                                                                         >  1:253708/1‑250 (MQ=255)                                                                             gggCGCGGGCGCTAAAGGTGGCGCACCGAGACCGTTTTACCACGCATCCAACCCCGAATG‑GCGGGGGCGTCATTGTCAAAGAGGGCAAAATGGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCGGAAGTACCGGCGTTGCTTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgcc                                                                                                                                                                    <  2:25309/250‑1 (MQ=255)                                                                                          aaaGCTGGGGAAACGAGGTCGTTTTCCCACGCATCCCCACCTGAGTG‑TCGGGTGCGTCATTGGCAAATATGGCGAAATTGTCGGTGAAGGTTCCCACCACCTTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGCGGGTGAAAAAGCCACAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcg                                                                                                                                                        <  1:426489/250‑1 (MQ=255)                                                                                          aaaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCACCCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgc                                                                                                                                                         <  1:201023/249‑1 (MQ=255)                                                                                                   gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGGCAAAGGGGCGACCGCCTATGTCACACTCGGAACCTGGAGCCATCAAGGTCGTACCGCAACGGGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTg                                                                                                                                              >  1:442916/1‑251 (MQ=255)                                                                                                     aaCGAGGACGTTTTACCACGCATCCCACCCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGcc                                                                                                                                            <  1:270299/251‑1 (MQ=255)                                                                                                     aaCGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGTGCGGCCGCCTATGTCACACTCGCACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCCCTGGCGCAGCGCGCCTGGTTGcc                                                                                                                                            >  1:297223/1‑251 (MQ=255)                                                                                                                tttcccACGCATCCCACCCCGAATG‑TGGGGGGAGTCATTGTCAAAGATGGCGAAATGGTCGGGGAAGGTTACCCCCAACGTGGGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTAGGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAg                                                                                                                                  <  2:260766/246‑1 (MQ=255)                                                                                                                   aCCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCCTCATGGGCGTACGCCACCGTGCTGTGACGCACCCATCGCCGCCGGCGGAGCGCGCGGGGGTGCCTCGATGCaaaaacc                                                                                                                              >  2:382532/1‑246 (MQ=255)                                                                                                                    acaccgcacccAACCCGAATGGTCGGGTGCGTCATTGTCAAAGATGGCAAAATTGTCGGTGAAGTTCACCCCCAACGTGGGGGTAAACCACAGGCGGAAGTCCACGCGTTGGGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATc                                                                                                                               <  2:260492/242‑1 (MQ=255)                                                                                                                     cACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGGGCGACCGCCCATGTCACACCCCAACCCTGCAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCCTCCCCCCCGGCGCCGCGCGCGTGGGTGCCTCCATGCCAGATCCt                                                                                                                             >  2:352977/1‑250 (MQ=255)                                                                                                                       cGCACCCCAACCCGAAGT‑TCGGGTGCGACATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTGGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                                                                                                                          <  2:46438/251‑1 (MQ=255)                                                                                                                                aCCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGACAAAGGGGCGACCGCCTATGTCACACTCGGACCCTGTAGCCAACATGGTCGTACGCCCCCGCGCTGGGACGCCCTCATCCCCGCTGGCGGAGCGCGCGTGGTTGCCTCCATGCAAGAACCTAAccccccgggc                                                                                                                 >  2:4550/1‑244 (MQ=255)                                                                                                                                     aaTG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCAAAAGGGCGGACGCCCATGTCCCACTCGAACCCCGGAACCCTCATGGGCGGACGCCACCGGGCGGGGACGCCCCCATCGCCGCTGGCGGAGCGCGCGTGGGTGCCTCCGTGCCAGATCCTAACCCCCAGGGCGC‑Tgg                                                                                                           >  1:425005/1‑251 (MQ=255)                                                                                                                                     aaTG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAAGGGGGACCCCCCATGTCACACTCGAACCCTTTAGCCAACCTTGGCGTACCCCCCCCTGCTGTGACCCCCTCATCGCCCCTGGCGGAGGGCGCCGGGGTGCCCCGATGCAATATTCTCACCCCCAgggcccttg                                                                                                            >  1:267006/1‑244 (MQ=255)                                                                                                                                      aTG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCAAAAGGGCGACCGCCCCTTTCACAACCGAAACCTGTAGCCCTCATGGGCGTACGCCACCCTGCTGTGACGCCCTCCACGCCGCCGGCGTAGCGCGCGCGGGTGCCTCGGTGCAACATCCTAAACCCCAGGGCGC‑Tggg                                                                                                          >  2:390664/1‑251 (MQ=255)                                                                                                                                        g‑TCGGGAGCGTCATTGTCAAAGATGGCGAAATTGACGGTGAAGGTTACCACCAACGTGCGGGTGGACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCAAAAGTGCGACCACCTATGTAAAAATCAAACCCCGTAGAAATCAAGGTCGTACGCCCCCGTGGTGTGACGCACCCATCGCCGCCGGCGGAGCGCCCGTGGGTGCGTCGATGCAAAATCCTAAccccccgggcgcggggcg                                                                                                         >  2:344504/1‑236 (MQ=255)                                                                                                                                                        gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCggct                                                                                         >  1:81940/1‑248 (MQ=255)                                                                                                                                                        gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCt                                                                                         <  2:51686/251‑1 (MQ=255)                                                                                                                                                             aGATGGCGCAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAACCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGAGGCAAGATCCCAACCCGCAGGTCGC‑TGGGGGTGGACTTTACCGTCTGCAAc                                                                                    >  1:456572/1‑251 (MQ=255)                                                                                                                                                                    gAAATGGTCGGTGAAGGTTACCACCAACTTGCGGGTGAACCACATGCCAAAGTACCCGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTgg                                                                             <  1:373449/251‑1 (MQ=255)                                                                                                                                                                           gcGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACTCGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGAc                                                                      <  1:152935/250‑1 (MQ=255)                                                                                                                                                                            cGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGTGCGACCGCCCATGTCACACTCCAACCCTGTAGCCATCATGGTCGTACGCCACCGCGCTGTGACGCCCTCATCGCCGCTGGCGTAGCGCGCGGGGTTGCCTCGATTCAAGACCCTAACCCCCAGGTCGC‑CGGGGGGGGGCTTTACCGTCTGCAACAGGCCGGccatga                                                                       >  2:90503/1‑244 (MQ=255)                                                                                                                                                                             ggTGAAGGTTACCACCAACGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                                    <  1:60635/251‑1 (MQ=255)                                                                                                                                                                              gTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCCTCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGGGGGGGTGCCTCGATGCAAGATCCTAACCCCCCGGGCGC‑TGGGCGGGGGCTTTACCGTCCGCAACAGGCTGGccgtgacggc                                                                   >  2:304770/1‑242 (MQ=255)                                                                                                                                                                                  aGGTTACCACCAACGTGCGGGTGAACCACATGCCGACGTACACGCGTTGCGTATGGCGGGCGAAAAAGCCAAAAGCGCGACCCCCTATATCACACTCGAACCCTGTAGCCATCATCGTCGTACGCCACCCCGCTGTGACGCACCCACACCCGCTGGCCTAGCGCGCGCGGTTGCCTCGATGCAAGACCCTAACCCGCAGGGCGC‑CGGGCGTGGGCTTTACCGTCTGCAACAGGCTGGCATTGACGGCAGcc                                                               >  1:325777/1‑251 (MQ=255)                                                                                                                                                                                     ttACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCAACGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                            >  1:312448/1‑251 (MQ=255)                                                                                                                                                                                         ccccaACGGGGGGGTGACCCACAGGCCGAAGACCACGTGTTGGGTAGGGCGGGGAAAAAAGCCAAGGGTCAGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCt                                                        <  2:305189/249‑1 (MQ=255)                                                                                                                                                                                             aaCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                    <  1:4550/251‑1 (MQ=255)                                                                                                                                                                                               ggTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGCGGGTGAAAAAGCCAAGGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatgat                                                  <  1:198961/250‑1 (MQ=255)                                                                                                                                                                                               cGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCCGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatgat                                                  >  1:331770/1‑251 (MQ=255)                                                                                                                                                                                                 ggggggtaacccacaggcgaaaGTACACGGGTTGCGTGTGGGGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatgatga                                                <  2:81940/231‑1 (MQ=255)                                                                                                                                                                                                 ggCGGGTGACCCACTGGCGGAAGTACCCGGGTGGCGTAGGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatgatga                                                <  2:231622/250‑1 (MQ=255)                                                                                                                                                                                                        gAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGGTGATGAGTGAAGc                                         >  1:243146/1‑251 (MQ=255)                                                                                                                                                                                                           ccACATGCCGAAGTACCCGCGTGGCGTAGGGCGGGGGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGa                                      <  2:447146/251‑1 (MQ=255)                                                                                                                                                                                                            cacaTGCCGAAGTCCACGGGTTGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                     <  1:401595/251‑1 (MQ=255)                                                                                                                                                                                                            cacaTGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGGGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                       >  1:255060/1‑249 (MQ=255)                                                                                                                                                                                                              caTGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCCAAAGTGCGGCCGCCTAATTTACACTCGAACCCTGTAGCCATCCTGGGCGTCCGCCACCGGGCTGTGACGCACTCATCCCCGCTGGCGGAGCGCGCGTGGGTGCCCCGCTGCAAGATCCTAACCCGCCGGTTGCCGGGGGGTGG‑CGTTACCGCCTGCAACAAGCTGGGATTGAAGTCAGCCACCGCCTGCTGTTGAGTggaaccgcgc                                    >  2:429163/1‑241 (MQ=255)                                                                                                                                                                                                                 gCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAAtt                                <  1:176036/251‑1 (MQ=255)                                                                                                                                                                                                                  ccGAAGTCCCCGCGTGGCGTAGGGGGGGGAAAAAACCCAAGGGGGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTg                               <  2:364608/251‑1 (MQ=255)                                                                                                                                                                                                                     aaGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCCAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTTACGTCAGCCACGGCCTGGTGATGAGTGAAGCCGAGCAAtttgat                            >  1:229798/1‑247 (MQ=255)                                                                                                                                                                                                                     aaGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAAt                            <  1:279834/251‑1 (MQ=255)                                                                                                                                                                                                                       gTACACGGGTTGCTTATGGCGGGTGAAAAAGCCAAAGTTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATaa                          <  1:7808/251‑1 (MQ=255)                                                                                                                                                                                                                            gcgtttGCGTAGGGCGGTTAAAAAGGCCTAGGGGGCTACCGCCTATGTCACCCTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCt                     <  2:451948/247‑1 (MQ=255)                                                                                                                                                                                                                                      aTGGGGGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGc            <  2:47611/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCg           <  1:140727/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATg       <  1:90503/251‑1 (MQ=255)                                                                                                                                                                                                                                            ggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATgcg     >  1:352668/1‑251 (MQ=255)                                                                                                                                                                                                                                             gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATgcgc    >  2:424806/1‑251 (MQ=255)                                                                                                                                                                                                                                               aaaaaaGCCAAGGAGGCGACCGCCTATGTCACACTCGAACCTTGTAGCCATCATGGTCGTCCGCCACCGTGCTGTGACGCAGTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGATCCTAGCCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACGGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAATGGCTTTCTCAAGCGGATGCGCAc  <  2:51458/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AGTTTCGAAGATATCAAAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATG‑TCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGC‑TGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAC  >  NC_000913/433398‑433885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑