| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,396 | 0 | C | T | 26.3% | 92.9 / 14.2 | 57 | S39F (TCT→TTT) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base C (16/26); new base T (15/0); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAG > NC_000913/119147‑119644 | gCACTGATTGATTTCTATCCGTAGAGCGCTAAAAGCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGGGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTTAAACATTTTtct < 2:309786/251‑1 (MQ=255) ttgattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAAATTTTTTTC‑TCgggg > 1:304009/1‑250 (MQ=255) tgattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTC‑TCGGgcg > 2:98407/1‑251 (MQ=255) gattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTC‑TCGGgcgc > 1:397748/1‑251 (MQ=255) tgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCAc < 1:53172/250‑1 (MQ=255) ccGGATATCGCTAAAAACATGACGGGCAATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACACCATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGTAACATTTTTTTC‑TTGGgcggccgtttggg > 2:356173/1‑241 (MQ=255) ggATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTC‑TCGGGCGCACGttttgcc > 1:410580/1‑245 (MQ=255) ggATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTTTT‑TCGGGCGCAccgtaggatg > 2:299422/1‑242 (MQ=255) cGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATACCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGTCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCa < 1:220361/250‑1 (MQ=255) aaaaaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCattatt < 1:433394/251‑1 (MQ=255) aaaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGtt < 1:373652/233‑1 (MQ=255) aaCATGACGGGCCATTGTGATATTGCGCCGGATCTGAAAACCGAGCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACACCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCg < 2:137010/251‑1 (MQ=255) cGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAAATTTTTTTC‑TCGGGGGCCCGTT‑AGGCCTGACCCTTATTGCGATggggg > 2:324873/1‑249 (MQ=255) gCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACCTT‑AGGCATGACAATTATTTCGATGGGCGTgg > 1:320398/1‑250 (MQ=255) atatTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTTTC‑TCGGGCGGCCGTT‑ATGCATGACCATTATTGCGGTGGGGGGG‑ACtttttttt > 2:349383/1‑249 (MQ=255) cGGATCGGAAAACCGA‑CCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACgcgc < 2:5829/251‑1 (MQ=255) aTCGGAAAACCGATCCCGGTCCAGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCAt < 2:142485/251‑1 (MQ=255) aCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCCGGCGCGCGTT‑AGGGATGAACATTATTGCGATGGGCGTG‑ACTTTTTTACTTTTACGCGCATTGCAgggg > 2:437081/1‑250 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑Ta < 2:337094/251‑1 (MQ=255) aTCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTTCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAggat > 1:118451/1‑248 (MQ=255) aTCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCCTTATTTCGATGGGCGTG‑GCTTTTTTTCTGTTACGCGCATTGCAGGGAG‑TA‑t > 1:136874/1‑251 (MQ=255) tCCTGCATTTGATTGGGCACGGATTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAACTTTTTCTC‑TCTGGCGCACGTT‑AGGGATGGCCCATGTTGCGATTGGTGTG‑TCTTTTTTTCTGTTTCGCGCATTGCAGGGGG‑TA‑TTGTTCa > 2:196633/1‑250 (MQ=255) tGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCa < 1:393351/250‑1 (MQ=255) ggCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACCTTTTTCTC‑TCGGGGGCCCGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTTCTGTTACGCGCATTGGAGGGAG‑GA‑TTGTTCAACGTTCCCAccccact > 1:230709/1‑245 (MQ=255) tCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGGTGGGGGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑GA‑TTTTTTAAAGTTTCCAAGCTACTGGTGTGgcg > 1:248507/1‑250 (MQ=255) gtacTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGCTGGGCGAGCACTGGCAGCTTTATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTTCTATGGGCGTG‑ACTTTTTTACTGTTACGCGCTTTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGgctgct < 1:171970/247‑1 (MQ=255) cAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGttt < 2:460773/251‑1 (MQ=255) cAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGAGCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCt < 2:426119/251‑1 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGGAACATTTTTCTCCTCGG‑CGCCCGTT‑TGGCATGACCCTTATTTCGATGGGCGTG‑GCTTTTTTTCTGTTTCGCGCCTTTCAGGGGG‑TAATTTTTCAA‑GTTCCCCCGCTTCTTGTGTGGCTGCTGGtttgtttgttt > 2:55093/1‑248 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTTTTACGCGCATTGCAGGGAG‑GA‑TTGTTTAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCtg > 1:160252/1‑251 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCtg < 1:257172/251‑1 (MQ=255) agaCAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTC‑TCGGGCGCACGTT‑AGGCCTGACCATTATTGCGAGTGGCGTG‑ACTTTTTTTATGTTTAGCGCATTGCAGGGAG‑TA‑TTGGTCAACGTTCCCACGCTCCTGGTGTGGGTGCTGATTGGTTTTCTgtgtt > 1:85042/1‑250 (MQ=255) acaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgc < 2:452860/251‑1 (MQ=255) aTTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTCAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGTAGCGTTCTTTCGGCGGGGGAAACATTTTTCTC‑TCGCGCGCACGTTCTGCCCTGCCCCTT‑TTTCGATCGGCGGGCATTTTTTTAC‑GTTACGCGCATTTTAACGAGGTA‑TGGTACAA‑GTTCCCACGCTACTGTGGTGGCTGCTGAGTGGGTTTCTGTGTATTTgcgccagatca > 2:415081/1‑243 (MQ=255) aaCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAGAAACATTTTTCTC‑TCGGGCGCACGGT‑TGGCATGGCCATTATTGCGGTGGGCGTG‑ACTTTTTTACTGTTACGCGCCTTGCAGGGAG‑TA‑TTTTTCAACGTTCCCCCGCTACTGGGGTGGCTGCTGATTGGGTTGCTGTGTATTGGCGCA‑GG‑GAAAGTTCGTc > 1:385595/1‑251 (MQ=255) gtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATg < 2:160252/251‑1 (MQ=255) gtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATTACCATTTTTGGGGTGGGGGGG‑ACTTTTTTTTTGTTACGCGCCTTGCCGGGAG‑GA‑TTGTTCAACGTTCCCACGCTACTGGGGTGGGTGCTGATTGGTTTGCTGTGTATTTGGGCA‑GG‑GAAAGGTTGTCTTcttttttatt > 2:175629/1‑242 (MQ=255) aaTTTTCGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGGCCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGTTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCtt < 2:468319/251‑1 (MQ=255) aTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGGAACAATTTTTTC‑TCGGGCGCACGGT‑AGGCATGACCATTATTGCGGTGGGCGTG‑GCTTTTTTACTGTTACGCGCATTGCCGGGAG‑GA‑TTTTTCAAAGTTTCCAAGCTACTGGTGTGGCTGCCGATTGGTTTGCTGGGGATTGGGGCAAGG‑TAA‑GTTTGTCTTCTTTATCATGCttt > 1:13102/1‑250 (MQ=255) ttttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGGAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCCTTATTGCGGTGGGGGTG‑ACTTTTTTTCTGTTACGCGCCTTGCAGGGAG‑GA‑TTGTTCTAAGGTCCCACCCTACCGGGGGGGCTGATGGTTGGTTTGCTGTGTATTTGCGCA‑GG‑GAAAGGTCGTCTTTCTTATCATGCtttt > 2:293752/1‑249 (MQ=255) tttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAt < 1:324873/250‑1 (MQ=255) tttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTAGTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAt < 2:385595/250‑1 (MQ=255) tCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑Ct < 1:124290/250‑1 (MQ=255) cGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACCTTTTTTTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGGGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑GA‑TTGTTCAACGTTCCCACGCTACTTGGGTGGCTGCTGGTTGGGTTGCTGTGTATTGGCGCA‑GG‑GAAAGTTCGGCTTTATTATCATGCTTAT‑CTg > 1:396297/1‑250 (MQ=255) aGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTC‑TCGGGCGCCCGGT‑AGGCATGACCCTTATTGCGATGGGCGGG‑GCTTTTTTTCTGTTATGCGCATTGCAGGGAG‑TA‑TTGTTCAACGGTCCCACGCTACTGGTGTGGCTGCTGATTGGGTTTCTGTGTATTGGCGCA‑GG‑TAAAGGTCGTCTTCATTTTCATGCTTAT‑CTGAc > 2:67432/1‑250 (MQ=255) cgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCAt < 1:262822/237‑1 (MQ=255) cgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAATTTTTTTC‑TCGGGGGCCCGTT‑TGGCATGACCCTTATTGCGATGGGCGTG‑ACTTTTTTTTTGTTTCGCGCATTGCAGGGGG‑TA‑TTGTTCAAAGTTCCCCCGCTACTGGGGTGGGGGCTGATTGGGTTTCTGTGGATTGGCGCC‑GG‑GAAAAGTCGTTTTCATTAttatagattggaagagtg > 2:262822/1‑234 (MQ=255) attAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACCTTTTTTTT‑TCTGGCGCACGTG‑AGGCATGACCCTTTTTGCGATGGGGGGG‑ACTTTTTTATTGTTATCCGCACTGCAGGGGG‑TA‑TTGATCAACGTTTCCCCGCCCTTGGTGGGGCTGCTGATTGGGTTTTTGTGTGTTTGCGCC‑GG‑GTAAGTTCGGCTGCGTTTTCATGCTTAttatacatcggcttc > 2:27971/2‑237 (MQ=255) aaGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTC‑TCGGGCGCACGTT‑TGGCATGACCATTATTGCGATGGGCGTG‑GCTTTTTTTCTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAAAGTTCCCACGCTACTGGTGTGGCTGCTGATTGGGTTTCTGTGTATTTGGGCA‑GGCAAAAGTT‑GTCTTCCTTTTCATGCTTATTCT‑ACAGCTGCCTCACg > 2:143611/1‑251 (MQ=255) gggCGAGCTCTGGCATCTTGATCATCGTCTTGAAGCATTCTTTCGGCGGGTGAAACATTTTTCTC‑TCTTGCTCACGTT‑AGGCATGGCCATTAGTGCGATGGGCGTG‑ACTTTTTTACTGTGACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGAGTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATTCTTAT‑CTTACAGCTTCTTCACGCAAt < 2:245295/250‑1 (MQ=255) cGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATa < 1:349383/251‑1 (MQ=255) cGAGCACTGGCAGCGTGATCATCGTCTTGAAGCGTTCGTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTGACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATa < 2:346617/251‑1 (MQ=255) atcGTCTTGAAGCGTTCTTTCGGCGGGTGGAACATTTTTTTC‑CCGGGCGCACATT‑TAGCATTACCATTAATGAGTCGGGCGTG‑GCTTTTTTTCTGTTTCGCCCCCTTCCGGGGG‑TA‑TTTTTCAACGTTTCCAAGCTACTGGGGGGGTTTCTGGTTGGTGTGGTTTGTATTTGCGCC‑GG‑GAAAATTCGGCTTCTTTATTACGCTTAT‑TTGTCAGCTTCCTCACGGAATAGTAGCCATGccctttccccgatg > 2:414966/1‑240 (MQ=255) gTCTTGAAGCGTTCTTTCGGCGGGTGAAGCATTTTTCTC‑TCGTGCGCACGTT‑AGGCGTGACCATTATTGCGATGGGCGTG‑ACTTTTTTTCTGTTACGCGTATTGCAGGGAG‑TA‑TTGTGCAACGTACCCGCGCTTCTGGTGTGGCTTCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑GAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATATCCATGCCCGTGCCACGAtggct < 1:181648/251‑1 (MQ=255) tctatCGGCGGTTGAAACATTTTTCTC‑TCTGGCGCACGTT‑ATGCATGACCATTAGTGCGATGGTCGTG‑ATCTTTTTACCTTTTCGCGCATTGCAGTGAG‑TA‑TTTTTCAACGTTCCCACGCTACTTGTGTGGCTGCTGATTGGTTTGCTGTGTATTGTCGCT‑GG‑TAAAGTTGGTCTTAATTATCATGCTTAT‑CTGTCAGCTGCTTCACGTAATTATAGCCATACCCGTGCCACGAGGGCTGGCTATCTCAc < 2:193414/246‑1 (MQ=255) gcggGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTc > 1:63371/1‑251 (MQ=255) aaCATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCAGTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGGTCGTCTTCATTAGCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGCTTCACGGCGTc < 2:378624/251‑1 (MQ=255) aTTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGGG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑CAACGTTTGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATCATAGCCATGCCCGTGCCACGATGGCCGCCGAACTCACCCTGATGCACGGCGAcccc > 2:61386/1‑250 (MQ=255) tctc‑tcGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTTCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAg < 1:26809/250‑1 (MQ=255) | GCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTC‑TCGGGCGCACGTT‑AGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAG‑TA‑TTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCA‑GG‑TAAAGTTCGTCTTCATTATCATGCTTAT‑CTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAG > NC_000913/119147‑119644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |