| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 2,498,768 | 9486 bp→CCC | [pyrS]–fryB | [pyrS], pyrR, ypdC, fryA, ypdE, ypdF, fryC, fryB | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 2498768 | 2508253 | 9486 | 33 [0] | [0] 33 | [pyrS]–fryB | [pyrS],pyrR,ypdC,fryA,ypdE,ypdF,fryC,fryB |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 2498767 | 0 (0.000) | 33 (0.560) +CCC |
30/488 | 0.6 | 100% | coding (97/1698 nt) | pyrS | sensor histidine kinase PyrS |
| ? | NC_000913 | 2508254 = | 0 (0.000) | intergenic (‑12/+207) | fryB/glk | putative PTS enzyme IIB component FryB/glucokinase | |||||
TGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAAT > NC_000913/2508012‑2508492 | cattatcaagagatgcattgctaatacaccatttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTg > 1:230595‑M2/243‑251 (MQ=255) atcaagagatgcattgctaatacaccatttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGAt > 1:133174‑M2/239‑249 (MQ=255) ccatttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgataatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTg > 1:51409‑M2/215‑251 (MQ=255) atttatctatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCa < 2:75669‑M2/39‑1 (MQ=255) tatcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctgatcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaactattcaacatgctgctggcggtcttcgctcgggcagcgttaatgcttttctgcctgttctttctcctccgtatccgcctgtttcgcgcccCGTTCAGAGATAGTTGACGAGTGACATTTTGTACGTGCAAAACaa > 2:79871‑M2/206‑250 (MQ=255) atcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAAt > 1:219780‑M2/205‑251 (MQ=255) atcaatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAAt < 2:353218‑M2/47‑1 (MQ=255) aatccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTTTGAACGTGCAAAACAAatccc > 2:303363‑M2/202‑249 (MQ=255) ccccttttaaaattgtggtttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGt > 1:74692‑M2/199‑251 (MQ=255) ttcctcaaaaacgtcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 2:385086‑M2/73‑1 (MQ=255) cggcttgctggtcatcagccttcaggttacctatcataaaggtttaatccttattcaaagtcccccgtgcacgaaatattcaacatgctgcgggcggtcttcgatcgggcagcgttaatgcttatctgcctgttttttcccatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGATAAtt < 2:239318‑M2/85‑1 (MQ=255) ggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt < 2:133174‑M2/94‑1 (MQ=255) catcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGt > 1:400282‑M2/155‑250 (MQ=255) gcttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATTCCGCTGTACGGAAGTGTGAc > 2:68480‑M2/143‑251 (MQ=255) atcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCg < 1:8836‑M2/117‑1 (MQ=255) atcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCg < 2:248678‑M2/117‑1 (MQ=255) atcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCg > 1:248678‑M2/135‑251 (MQ=255) atcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcgtcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCg < 2:400282‑M2/117‑1 (MQ=255) atagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGAcc > 2:394698‑M2/132‑251 (MQ=255) tttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGGGTGACGCAGGTCGACCTtgtg > 1:323539‑M2/125‑249 (MQ=255) cagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGaa < 1:407369‑M2/140‑1 (MQ=255) gcacgaaatattcaacatgctgctgacggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGCTAGTTGACGAGTGAACTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGGGACGCAGGACGACCTTGTGCGGGGACACAAAGAAAAATTGAATaaa > 1:66001‑M2/99‑250 (MQ=255) cgaaatattcaacatgctgctggctgtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGt < 2:435210‑M2/156‑1 (MQ=255) gctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATc > 1:460830‑M2/78‑251 (MQ=255) gctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTGACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGAtt < 1:437006‑M2/171‑1 (MQ=255) gggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCg < 2:387210‑M2/191‑1 (MQ=255) atctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAt < 1:72121‑M2/207‑1 (MQ=255) ctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAttt > 2:125367‑M2/42‑250 (MQ=255) tctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGAcc > 2:24809‑M2/30‑251 (MQ=255) tcccatccttatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCACCCGATATAAAAGGAAGGATTTACAGAATGTGAc < 2:28314‑M2/221‑1 (MQ=255) ctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCt > 1:5815‑M2/29‑251 (MQ=255) catccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTa > 1:448279‑M2/27‑250 (MQ=255) tgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAAt > 1:152789‑M2/13‑251 (MQ=255) | TGATTCGAAACGTTCGTTATCTTCCGGGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAAT > NC_000913/2508012‑2508492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |