| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,173,431 | T→A | I97N (ATC→AAC) | ygiN → | putative quinol monooxygenase YgiN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,173,431 | 0 | T | A | 98.7% | 286.4 / NA | 77 | I97N (ATC→AAC) | ygiN | putative quinol monooxygenase YgiN |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (37/39); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTT > NC_000913/3173187‑3173670 | aaacggcaacgcacggtccatgatcacgtacatggcgtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgatccgacggcgataaaggcatcaGAATAACcgtct > 2:530139‑M2/240‑249 (MQ=255) aacgcacggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAg < 1:403541‑M2/19‑1 (MQ=255) acgcacggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGc < 1:504580‑M2/20‑1 (MQ=255) cgcacggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtcaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggcgatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGcc > 2:519041‑M2/231‑251 (MQ=255) cggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAggg < 1:303118‑M2/25‑1 (MQ=255) ggtccatgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGCATTCTGCAGCCAggg > 2:287795‑M2/226‑250 (MQ=255) catgatcacgtacatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGAttt > 2:26012‑M2/222‑250 (MQ=255) acgtacatggagtagtttaagaaggcgttttccggaaatgcaggtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagaacaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGTGATTTAATccttc < 2:370078‑M2/37‑3 (MQ=255) acatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTg < 1:320697‑M2/41‑1 (MQ=255) acatggagtagtttaagtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTg > 1:257015‑M2/211‑251 (MQ=255) ggatgagtttaagtagtcgtgttccgtaattccatgtagggcaaggcgctctgccatatggctcattaagtctgatgccgctacttattcgccagccttcaaaggggcaatattgccggagatacaacattatcggccgcgtcgagtcacaggagatcaacgtgaatagctccgccgggtgtgctccgacggcgataaaggcatcaTAATATCCGTATTCTGCATCCAGGGATTTAATCCTGCCTTGttt < 2:44302‑M2/44‑1 (MQ=255) agtaggctttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATcc < 1:356591‑M2/56‑1 (MQ=255) gtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCt > 1:319967‑M2/195‑251 (MQ=255) gtaggcgttttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattccacaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCt < 2:225168‑M2/57‑1 (MQ=255) ttccgtaaattcatgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGc < 2:465722‑M2/65‑1 (MQ=255) catgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTcc < 1:637858‑M2/77‑1 (MQ=255) catgtagcgcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatagtgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTc < 1:239108‑M2/76‑1 (MQ=255) gcaaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAAcc > 2:126201‑M2/167‑251 (MQ=255) gcaaggcgctctgccatatcgcacattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggaaatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAAcc > 1:199609‑M2/167‑251 (MQ=255) caaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCt > 2:370887‑M2/166‑251 (MQ=255) caaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCt > 2:89996‑M2/166‑251 (MQ=255) caaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCt > 2:541295‑M2/166‑251 (MQ=255) aaggcgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTc < 1:547433‑M2/87‑1 (MQ=255) cgctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAAtc < 2:605161‑M2/91‑1 (MQ=255) gctctgccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAAtct < 1:511354‑M2/92‑1 (MQ=255) gccatatcgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTcc < 2:83343‑M2/94‑1 (MQ=255) atatcgcttattaattctgattcctcaactgattcgccagacttcaaaggggcaatattgccggggatcctacattatctgccgcgtcgagtcacaggagatcaaagtgaatagcgccgccggacgttctcagacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGGTCTTTCATTAAAACCTCAATCTCCGCCATg < 2:467955‑M2/100‑1 (MQ=255) tcgctcattcgttctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCg > 2:185754‑M2/149‑251 (MQ=255) cgctcattaattctgattcctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCATATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGc > 1:216640‑M2/148‑251 (MQ=255) cctcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGt < 2:40040‑M2/122‑1 (MQ=255) tcaacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTg > 1:51815‑M2/128‑251 (MQ=255) ccaacttattcgccagccttcaaaggggaaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTg < 2:113525‑M2/124‑1 (MQ=255) aacttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCACCATGTCGCCTTTCTCTTGCAACCAGTTgcg > 1:418421‑M2/126‑251 (MQ=255) cttattcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaccgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTACACCTCTATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTgcggc > 2:273165‑M2/124‑251 (MQ=255) tcgccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATc < 1:129584‑M2/132‑1 (MQ=255) gccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTc > 1:396263‑M2/117‑251 (MQ=255) ccagccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCACTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTcc > 2:388868‑M2/116‑251 (MQ=255) ccttcaaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGc < 1:375629‑M2/140‑1 (MQ=255) aaaggggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTttc < 1:325879‑M2/145‑1 (MQ=255) ggcaatattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGc < 2:343443‑M2/150‑1 (MQ=255) atattgccggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCcagc > 2:384840‑M2/98‑251 (MQ=255) ggagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATc > 1:230200‑M2/90‑251 (MQ=255) gagatactacattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATcc < 1:286402‑M2/163‑1 (MQ=255) atactccattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCcatc > 1:316503‑M2/86‑251 (MQ=255) gcatcatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCCTCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTATTCGCCAGCAGCATCTCCATCATcg < 2:342112‑M2/170‑1 (MQ=255) acattatctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCatcatc < 2:53640‑M2/169‑1 (MQ=255) atctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGCATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCCCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTc > 1:30263‑M2/76‑249 (MQ=255) atctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTc > 1:563764‑M2/76‑249 (MQ=255) ctgccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacgacgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGt > 1:549687‑M2/74‑250 (MQ=255) ggccgcgtcgagtcacaggagatcaaagtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAGACCTCAATCTCCGCCATGTCGCCTTACTCGTGCAACCAGTTGCGGCGATCTTCCGGGCGTTTCTTCGCCATCAGCATAGCCATCATCGCGTCAGTAc > 2:435076‑M2/73‑251 (MQ=255) ccgcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGc < 1:311928‑M2/181‑1 (MQ=255) gcgtcgagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg < 1:367208‑M2/183‑1 (MQ=255) tcgagtcacaggagatcaacgtgaatagcgccgccggaagtgccccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTAAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGCGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTAAGTACGCTGATc > 2:163962‑M2/66‑251 (MQ=255) cgagtcacaggagatcaacgtgaatagcgcagccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCATCCAGTGATTTATGCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCTACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATc < 2:271746‑M2/186‑1 (MQ=255) cgaggcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCTGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCTCCTTTCTCTTGCAACCGGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATc < 1:468557‑M2/186‑1 (MQ=255) gagtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGt > 1:207402‑M2/64‑251 (MQ=255) agtcacaggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTc > 2:511415‑M2/63‑251 (MQ=255) caggagatcaacgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCAATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcat > 2:149092‑M2/58‑251 (MQ=255) ggagatcaacgtgaatagcgccgccggccgggctccgacggcgataaaggcatctGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGCCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcatca < 2:77106‑M2/196‑1 (MQ=255) cgtgaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCTTCTTCATCATCTATAGTc < 1:149119‑M2/205‑1 (MQ=255) gaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCaa < 1:459510‑M2/207‑1 (MQ=255) gaatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAAc < 1:236460‑M2/208‑1 (MQ=255) aatagcgccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACt < 1:126201‑M2/209‑1 (MQ=255) gccgccggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAcc > 1:397741‑M2/37‑250 (MQ=255) cggacgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCCGACgg > 2:431132‑M2/32‑251 (MQ=255) ggacgtgctccgacggcgctaaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCAATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGAACGCAGATCGTCTTCATCATCGATAGTCACCTGCACCAGACGGc > 2:322566‑M2/31‑251 (MQ=255) acgtgctccgacggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGa < 1:613911‑M2/223‑1 (MQ=255) ggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATc > 2:604696‑M2/17‑250 (MQ=255) ggcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCa < 1:231005‑M2/235‑1 (MQ=255) gcgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTTGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCaa < 2:532082‑M2/236‑1 (MQ=255) cgataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGATCGGATCa > 1:108204‑M2/15‑249 (MQ=255) gataaaggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGc < 1:573781‑M2/238‑1 (MQ=255) gtcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCGGCCATGTTGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGTGAGCGTTCGGATCAAGCGTGGtt > 1:336997‑M2/8‑251 (MQ=255) ggcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGtt > 1:337001‑M2/8‑251 (MQ=255) gcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCATCCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGttt < 2:508511‑M2/245‑1 (MQ=255) gcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGttt < 1:33166‑M2/245‑1 (MQ=255) gcatcaGAATAACCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCACGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCGGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGTTAGTCAACTGCACCAGTCGGCGAGTGTTCGGATCAAGCGTGGttt > 2:113880‑M2/7‑251 (MQ=255) | CACCGTCAGGCGGTATTGGATCAGTTTGCTAAAATCGTTCCAACCGTACTGAAAGAAGAAGGTTGCCACGGCTATGCGCCAATGGTGGATTGCGCAGCTGGCGTGAGTTTCCAGTCTATGGCACCGGATTCTATCGTGATGATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAAGCGTGGTTT > NC_000913/3173187‑3173670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |