Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,153	C→T	P58S (CCC→TCC)	lhgD →	L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,153	0	C	T	100.0%	221.7 / NA	65	P58S (CCC→TCC)	lhgD	L‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/24);  total (41/24)

TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTT  >  NC_000913/2789907‑2790393                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 aggcccaacgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATTATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCGGTCCCCAGACGGGCCACAACAGCGTCGTGATCCATGCCGGGGTCTATTACACGTCCg                                                                                                                                                                                                                                               <  1:137160‑M2/194‑1 (MQ=255)        acgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGCGTGGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTg                                                                                                                                                                                                                                       <  2:219590‑M2/202‑1 (MQ=255)           aaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGGGATCCATGCCGGGGGCTATTCCACGTCCGGCAGCCTGa                                                                                                                                                                                                                                      >  2:407226‑M2/47‑249 (MQ=255)            aaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCCACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGCAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTACATTACACGTCCGGCAGCCTGAAgg                                                                                                                                                                                                                                   >  2:567988‑M2/46‑251 (MQ=255)                 tctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGCTTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGCGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTCCACGTCCGGCCACCCGAAGgcgc                                                                                                                                                                                                                                >  2:100162‑M2/41‑249 (MQ=255)                 tctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGgcgc                                                                                                                                                                                                                                >  1:623672‑M2/41‑249 (MQ=255)                        tttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGcc                                                                                                                                                                                                                       >  1:274034‑M2/34‑251 (MQ=255)                          tacgttgggcctaaactcagggagactccccGCGCAAAGGAATTGAGCGGAGGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTccggcagcctgaaggaaaaatgtggatt                                                                                                                                                                                                                      <  1:333751‑M2/219‑14 (MQ=255)                             gttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCCGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGAGCAGTTTTGCCTGGCg                                                                                                                                                                                                                  >  1:198694‑M2/29‑251 (MQ=255)                                gggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCggg                                                                                                                                                                                                                <  1:121260‑M2/225‑1 (MQ=255)                                  gcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGCTTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGCTGTCTATCCGGCCGCCCGCATTGCGTTGCTGGAAAAAGAGGCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGAGCGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCCGTTTTGCCTGGCGGGaa                                                                                                                                                                                                              >  2:338732‑M2/24‑250 (MQ=255)                                    ctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGATGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACCGCGGCGTGATCCACGACGGGGGCTATTACAACTCCGGCAGCCCGAAGGCGCAGGTTTGCCTGGCGggaaaac                                                                                                                                                                                                           >  2:176704‑M2/22‑249 (MQ=255)                                        acgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCCGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGACGGCGCAGTTTTTCCTGGCGGGAAACCgcgc                                                                                                                                                                                                       >  2:249909‑M2/18‑251 (MQ=255)                                         cgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGcc                                                                                                                                                                                                      >  1:505397‑M2/17‑251 (MQ=255)                                                        cGCGCAAAGGAGTTGTGCGTAGTTATGATTTTGTGATTTGTGGCGGCGGTACCATCGGCATGTCGCCCGCAATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGc                                                                                                                                                                                       <  2:215387‑M2/250‑1 (MQ=255)                                                        cGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGc                                                                                                                                                                                       >  1:486617‑M2/2‑251 (MQ=255)                                                         gcgcAAAGGAATGGGGCGGATTTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCg                                                                                                                                                                                      <  1:430142/251‑1 (MQ=255)                                                            cAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTACCCTGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATc                                                                                                                                                                                   >  1:592339/1‑251 (MQ=255)                                                             aaaGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCCCGCCACCAAAGCCCTTTGCGATc                                                                                                                                                                                   >  2:222400/1‑250 (MQ=255)                                                             aaaGGAATTGAGCGGATGTATGATTTTGTGATGATTGGCGGCGGCAACATAGGAATGTCGTCCGCCATGCAACTGATTGATGACTATCCGGACGACCGCATTGCGTTGCTGGAAAAAGAGACCGCACCGGCCTGTCACCAGACGGGCCACAAAAGCGGCGAGATCCATGCAGGGGTCTATTACACGTCCGGGAGCCTGAAGGCGCAGGTTTGACTGGCGGGAAACCCCGCCACAACAGCCCTTTGCCATc                                                                                                                                                                                   >  2:342271/1‑250 (MQ=255)                                                                ggAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGGGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGGTTTGCCTGGCGGGAAACCGCGCCCCCAAAGCCTTTTGCGGTCaaa                                                                                                                                                                                >  2:288743/1‑250 (MQ=255)                                                                 gAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCAGGCAACTGATTGACGTCTATCCGGACGCCCGCATGGCGTCGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaaagc                                                                                                                                                                              <  2:196112/251‑3 (MQ=255)                                                                   aTTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACgg                                                                                                                                                                            >  1:557075/1‑251 (MQ=255)                                                                                     tttGTGATTATTGGCGGCGGCATCACCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACt                                                                                                                                                           <  1:548037/250‑1 (MQ=255)                                                                                        gtgATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGCAAACGCGCCACCACAGCCTTTTGCGATCAAAACGGCATTCGCTACGACCAATGCgg                                                                                                                                                       >  1:539671/1‑251 (MQ=255)                                                                                         ggATTATTGGCGGCGGCATCACCGGCTTGTCGACCGCCATGCAACGGAGTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGc                                                                                                                                                      <  2:404855/250‑1 (MQ=255)                                                                                             tattGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAg                                                                                                                                                   <  1:407226/250‑1 (MQ=255)                                                                                              attGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGa                                                                                                                                                  <  1:372878/250‑1 (MQ=255)                                                                                              attGGCGGCGGAATCATCGGCAGGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCTTTGCTGGAAAAAGAGCCCGCCCCGCCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGAt                                                                                                                                                 <  2:628182/251‑1 (MQ=255)                                                                                                 ggcggcggcATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCCGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCt                                                                                                                                              >  1:70679/1‑251 (MQ=255)                                                                                                     gcggcATCATCGGCAGGTCGACCGCCATGCAACTGTTTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCGGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGtt                                                                                                                                          <  1:282152/251‑1 (MQ=255)                                                                                                      gggaaccatcGGCATGTCGACCGCCATGCAACTGATTGATGTCTACCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTg                                                                                                                                         <  2:609722/245‑1 (MQ=255)                                                                                                                   aTGTCGACCTCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtct                                                                                                                            <  1:293980/251‑1 (MQ=255)                                                                                                                         aCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGCCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAAt                                                                                                                      <  1:249909/251‑1 (MQ=255)                                                                                                                          ccGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATg                                                                                                                     >  2:384974/1‑251 (MQ=255)                                                                                                                           cGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATgg                                                                                                                    >  2:426201/1‑251 (MQ=255)                                                                                                                                       gattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcgcg                                                                                                        >  2:73137/1‑251 (MQ=255)                                                                                                                                            atGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAAATGCTGGTTGCCACGCCCGATCTCGAAATGGAACGGATGCGCGCCttt                                                                                                   >  2:283767/1‑250 (MQ=255)                                                                                                                                             tGTCTACCCGGAGGCCCGCATGGGGTTGCGGAAAAAAGAGTCCGCCCGGGCTGGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTa                                                                                                   <  2:138969/250‑1 (MQ=255)                                                                                                                                               tCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATgg                                                                                                >  1:378830/1‑251 (MQ=255)                                                                                                                                                    ccGGACGCCCGCATTGCGTTGCTGGCAAAACAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCGACGACAAATGCGGCAAGATGCTGGTTGACACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGGACg                                                                                           >  1:173108/1‑251 (MQ=255)                                                                                                                                                                   gCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACg                                                                             <  1:16854/250‑1 (MQ=255)                                                                                                                                                                    cGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAATCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                           >  1:220892/1‑251 (MQ=255)                                                                                                                                                                    cGTTGCTCGACAAAGAGTCCCCCCCGGTCTGTCACCAGACGTGTCACAACAGCGGCGTGATCCATGACGGGGCCTATTACACGTCAGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGACACCGCGCCACCCAAGCCTTTTGCGATCATAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCCCGTCCGATCTAGAAATGGAACGGATGCGCGCCTTATGGGCACGCACAGTGGCGAACggg                                                                           >  2:544738/1‑250 (MQ=255)                                                                                                                                                                       tGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTAt                                                                         <  1:268501/250‑1 (MQ=255)                                                                                                                                                                        gcgggAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAAc                                                                                            <  1:131749/227‑1 (MQ=255)                                                                                                                                                                        gCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAAc                                                                                            >  2:131749/1‑230 (MQ=255)                                                                                                                                                                         cTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCg                                                                       >  1:213687/1‑250 (MQ=255)                                                                                                                                                                              aaaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACCGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGcgcg                                                                 >  2:107400/1‑251 (MQ=255)                                                                                                                                                                                  gagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGGACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATg                                                             >  2:273932/1‑251 (MQ=255)                                                                                                                                                                                        gCCCCGGCCTGACACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGAGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTAAGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCTAAATGGAACGGCTGCGAGCCTTAGGGGAACGCACAGTAGCGAACGGTATCGAGCGCGAATGGCTTa                                                        >  2:330268/1‑250 (MQ=255)                                                                                                                                                                                             ggCCTGTCACCGGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcg                                                  <  2:623672/251‑1 (MQ=255)                                                                                                                                                                                                    cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGGTCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGGTCTCGAAATGGAACGGATGCGCGCCTTTTGGGAACGCACAGTGGCGAACGGTATCGAGAGCAAAGGGCGTAACGTcgactgact                                           >  2:245063/1‑246 (MQ=255)                                                                                                                                                                                                    cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGCGCGCGAATGGCTTAACGCCGACGaa                                             >  2:573456/1‑249 (MQ=255)                                                                                                                                                                                                       cAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcg                                        >  1:502691/1‑251 (MQ=255)                                                                                                                                                                                                        aGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgc                                       >  1:284538/1‑251 (MQ=255)                                                                                                                                                                                                        aGACGGGCCACAACAGCGGCGTGATCCATGACGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGCCGAAATgcgc                                       >  2:488133/1‑251 (MQ=255)                                                                                                                                                                                                                 acaacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACCAACTgcgcgagcgcgc                               >  2:28425/1‑249 (MQ=255)                                                                                                                                                                                                                              gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCg                 >  1:466940/1‑251 (MQ=255)                                                                                                                                                                                                                                tgcATGCCGGGGACTATTACACGTCTGGCAGCCTGAAGGCGCAGGTTTGCCTGGCTGGAAACTTCGCCACGAAAGACTTTTGCGATCAAAACCGCATTCGCTACCACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCCTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCCCCAGCGCGAACCGAATCACACCggg               >  2:200733/3‑251 (MQ=255)                                                                                                                                                                                                                                   aTGCCGTGGTCTATTACACGTCCGGCATCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTACGGCAAGATGCTGGTTGCCACGTCCGCTCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGGGAACCGAATATCACCGGGCTc            >  2:72210/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTc            <  1:222400/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTc            <  1:83207/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcg           <  1:518774/250‑1 (MQ=255)                                                                                                                                                                                                                                             cTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAttt  >  2:179628/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTT  >  NC_000913/2789907‑2790393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑