Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,417,047	A→C	intergenic (+156/‑34)	pta → / → yfcC	phosphate acetyltransferase/putative transporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,417,047	0	A	C	100.0%	255.3 / NA	69	intergenic (+156/‑34)	pta/yfcC	phosphate acetyltransferase/putative transporter YfcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/47);  total (22/47)

GGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATCACTGAATCCAAACCAACAAGAAGATGGGCAATGCCCGATACGTTGGTGATTATCTTTTTTGTTGCTATTTTAACCAGCCTTGCCACCTGGGTAGTTCCGGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGA  >  NC_000913/2416804‑2417292                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ggTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAAGTGCGCCATCTCACTTTGCATACTTCCCACttt                                                                                                                                                                                                                                                <  2:250487/251‑1 (MQ=255)        gACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacat                                                                                                                                                                                                                                         <  2:355897‑M1/251‑5 (MQ=255)         aCCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacata                                                                                                                                                                                                                                        <  2:582283‑M1/251‑6 (MQ=255)            tGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataacc                                                                                                                                                                                                                                     >  2:314817‑M1/1‑243 (MQ=255)            tGTCCCGTGACGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataacc                                                                                                                                                                                                                                     >  2:312201‑M1/1‑243 (MQ=255)                ccGTGGCGCTCTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCCCAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTTCGCCCTCTCACTTTGCATACTTCCCACTTTGTTacataaccctca                                                                                                                                                                                                                                 <  1:318575‑M1/251‑13 (MQ=255)                      cgcACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaa                                                                                                                                                                                                                           <  1:222216‑M1/251‑19 (MQ=255)                       gcACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGCTTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcacgtgaa                                                                                                                                                                                                                           >  2:197249‑M1/1‑232 (MQ=255)                       gcACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCCCTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaa                                                                                                                                                                                                                          <  1:615327‑M1/251‑20 (MQ=255)                            ggTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTACCCACTTTGTTacataaccctcaagtgaaaatg                                                                                                                                                                                                                       <  1:458527‑M1/249‑23 (MQ=255)                             gTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggct                                                                                                                                                                                                                    <  2:508462‑M1/251‑26 (MQ=255)                               tGACGATGTCGTCTACACCATCGCGCTGACGGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCAGCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTGTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGACATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggctt                                                                                                                                                                                                                   <  2:111134‑M1/250‑27 (MQ=255)                                gACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggctttt                                                                                                                                                                                                                 >  1:532434‑M1/1‑223 (MQ=255)                                gACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttt                                                                                                                                                                                                                  <  2:256841‑M1/250‑28 (MQ=255)                                 aCGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACtt                                                                                                                                                                                                                                                 <  1:154906/218‑1 (MQ=255)                                 aCGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACtt                                                                                                                                                                                                                                                 >  2:154906/1‑218 (MQ=255)                                 aCGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttt                                                                                                                                                                                                                <  1:629555‑M1/251‑30 (MQ=255)                                  cGATATCGTCTACACCATCGCGCTGACTGCGAGTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTGTGTTacataaccctcaagtgaaaatggctttttt                                                                                                                                                                                                               <  2:72005‑M1/251‑31 (MQ=255)                                       gcgTCTACTCCATCGCGCTGACTGCGATTCAGTCTGCAGAGCAGCAGTAAGCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAAGCACTATTTCCGGTTTTTTATGCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACGGTGCTGCAGCAATGAATTGCGCCATCTCACTTTGCATACTTCCCTCCTTGGTacatatccctcaagtgaaaatggctttttgatgtt                                                                                                                                                                                                          <  2:435046‑M1/250‑36 (MQ=255)                                              cacCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGATCCTGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtatt                                                                                                                                                                                                   <  1:376564‑M1/251‑43 (MQ=255)                                                  aTCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgt                                                                                                                                                                                               >  2:396643‑M1/1‑205 (MQ=255)                                                    cgcgCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgtt                                                                                                                                                                                              <  2:470745‑M1/250‑48 (MQ=255)                                                               cGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcag                                                                                                                                                                                  <  2:11872‑M1/251‑60 (MQ=255)                                                                   tCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggct                                                                                                                                                                               <  1:133730‑M1/250‑63 (MQ=255)                                                                          gCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttc                                                                                                                                                                          <  1:362668‑M1/248‑68 (MQ=255)                                                                                    agTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttat                                                                                                                                                             <  1:422099‑M1/251‑81 (MQ=255)                                                                                            cGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCTCTATTTCCGGTTTTTTATGCTCTTAAGTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgttttgtgtgtttgcaggctctttcatgcaggtcgtatgccatctt                                                                                                                                                     <  2:614042‑M1/251‑89 (MQ=255)                                                                                                   atcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgac                                                                                                                                               >  1:185761‑M1/1‑156 (MQ=255)                                                                                                    cccgCAGCTGTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgacatcttgacgacaa                                                                                                                                             <  2:292009‑M1/250‑97 (MQ=255)                                                                                                     ccGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaa                                                                                                                                             <  1:300103‑M1/250‑97 (MQ=255)                                                                                                         aGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCAGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCCTCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaacatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagt                                                                                                                                         >  1:508779‑M1/1‑150 (MQ=255)                                                                                                          gCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaa                                                                                                                                       <  2:372932‑M1/251‑103 (MQ=255)                                                                                                             ttGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaac                                                                                                                                      <  1:333748‑M1/249‑104 (MQ=255)                                                                                                              tGCGCTTCGGATATCTGAACCGGAAATAATCGCTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGACTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACGTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttctttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcagttcttatgccatctttacgacaacagtaacat                                                                                                                                    <  2:340034‑M1/250‑106 (MQ=255)                                                                                                              tGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCGTCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcagttcttatgccatcttgacgacaacagtaacat                                                                                                                                    <  2:387632‑M1/250‑106 (MQ=255)                                                                                                               gcgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattc                                                                                                                                  <  1:419953‑M1/251‑108 (MQ=255)                                                                                                                 gcTGCGGATATCTGAAACGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaa                                                                                                                                <  2:242849‑M1/251‑110 (MQ=255)                                                                                                                    gCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaac                                                                                                                               <  2:582473‑M1/249‑111 (MQ=255)                                                                                                                     cGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgtt                                                                                                                            <  2:633259‑M1/251‑114 (MQ=255)                                                                                                                         tatCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtatt                                                                                                                                                                                                   <  2:98543‑M1/176‑43 (MQ=255)                                                                                                                         tatCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtatt                                                                                                                                                                                                   >  1:98543‑M1/1‑134 (MQ=255)                                                                                                                                 cctgaaaTAATCTCTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTTATTAGCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacagaaccctcaagtgaaaatggcttttttaggttgtgtattgtgttgtgtgtttgcaggctctttcatgcatggcttatgccatcttgacaacaacagtaacattcaacgtgaaatatgttaat                                                                                                                <  2:492065‑M1/248‑126 (MQ=255)                                                                                                                                     aaaTAATCACTATTTCCGGTTTGTTAGTCTCTTAATTTGAATTAATCCTTTCTGATTATCTTTCTTAACTTCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcagactctttcatgcaggtcttatggcatctagacgacaacagtaacattcaacgttaaatatgttaataaga                                                                                                            <  2:234191‑M1/251‑130 (MQ=255)                                                                                                                                       ataatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacg                                                                                                          >  2:551514‑M1/1‑120 (MQ=255)                                                                                                                                         aatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgtt                                                                                                        <  1:137857‑M1/251‑134 (MQ=255)                                                                                                                                         aatCACTATTTCCGGTTTTTTAGTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggcgctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgtt                                                                                                        <  1:465842‑M1/251‑134 (MQ=255)                                                                                                                                                       gTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctg                                                                                          <  1:563008‑M1/251‑148 (MQ=255)                                                                                                                                                              aTTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttg                                                                                    <  1:435434‑M1/250‑154 (MQ=255)                                                                                                                                                                     tAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTTCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatag                                                                             <  1:234109‑M1/250‑161 (MQ=255)                                                                                                                                                                     tAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagc                                                                            >  1:279821‑M1/1‑90 (MQ=255)                                                                                                                                                                         ttGCATTAATCCTTTCTGATTATCTTGCTTACCTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatattgacgacaacagtaacattcaacgttaaatatgttactaagacgttgcattattgtcctgaagttgacgatagaaggt                                                                        >  2:591305‑M1/1‑86 (MQ=255)                                                                                                                                                                          tGCACTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggt                                                                        <  2:619270‑M1/250‑166 (MQ=255)                                                                                                                                                                                aaTCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcgg                                                                 >  1:374984‑M1/1‑79 (MQ=255)                                                                                                                                                                                aaTCCTTTCTGATTATCATGCTTAACTGCGCTGCATCAATGAATTGAGCCATCTCACTTTGCATACTTCACACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttactaagacgttgcattattgtcctgaagttgaagatagcaggtatagcgg                                                                 >  1:160198‑M1/1‑79 (MQ=255)                                                                                                                                                                                          gATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcg                                                         <  1:395495‑M1/249‑181 (MQ=255)                                                                                                                                                                                          gATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatc                                                          <  1:155279‑M1/248‑180 (MQ=255)                                                                                                                                                                                              aTCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcg                                                    <  2:8654‑M1/250‑186 (MQ=255)                                                                                                                                                                                                    cTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggaatagcggttgaatcgcagcgtttcgat                                             >  1:344354‑M1/1‑59 (MQ=255)                                                                                                                                                                                                      tAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattg                                           >  2:398214‑M1/1‑57 (MQ=255)                                                                                                                                                                                                        aCTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattga                                          >  1:444669‑M1/1‑55 (MQ=255)                                                                                                                                                                                                            cgcTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggc                                     <  1:629903‑M1/251‑201 (MQ=255)                                                                                                                                                                                                                         aaTTGCGCCATATCACTGTGCATACTTCCCACTTTGTTacataaccctcaagggaaactggcttttttacgttgtgcattgtgttgtgtgtttgcaggctatttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgctgcattattatcctgaagttgaagatagcagggatagcggttgaatcgcagcgtttcgattgaatggcagaacaaaaatgt                        >  1:121253‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                               gcCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgagga                  <  1:534664‑M1/251‑220 (MQ=255)                                                                                                                                                                                                                                ccATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgaggat                 >  1:404370‑M1/1‑31 (MQ=255)                                                                                                                                                                                                                                 catctcgctttgcatGCTTCCCACTTTGTTacataaccctcaagtggaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgacatcttgtcgacaacagtatcattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgatagaatgacagaagcaaaatgtagaggatt                <  2:131965‑M1/244‑222 (MQ=255)                                                                                                                                                                                                                                 cATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaaaggcagaaacaaaatgtcgaggatt                >  2:550662‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                                      cACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgaggatttgatc           <  1:94642‑M1/251‑227 (MQ=255)                                                                                                                                                                                                                                        cTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgaggatttgatcgc         >  1:420001‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                                                aCTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgaggatttgatcgcggttgca  >  1:308483‑M1/1‑15 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATCACTGAATCCAAACCAACAAGAAGATGGGCAATGCCCGATACGTTGGTGATTATCTTTTTTGTTGCTATTTTAACCAGCCTTGCCACCTGGGTAGTTCCGGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGA  >  NC_000913/2416804‑2417292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑