Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	98.7%	291.8 / NA	78	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (52/24);  minor base C (1/0);  total (53/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674272‑674762                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      aCTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACAGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGcc                                                                                                                                                                                                                                                   >  2:635005/1‑251 (MQ=255)             gCCAGTACGATCGGGTCGTTCGGAAACCAGTTGACCGCACAAGTCTTCTTATATACCAGGCCTTATTTA‑AACAGCTCGGTGACGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGACTCCGGTCATAACAAAAGCCCAGCATTTTGAGCTGGTTTATCATATACAAGATGTTGTA‑GTACGTCCACGGTACCGGAG‑CGGTCTTGTTTTTCACCGCCCAGCCATCCgcagac                                                                                                                                                                                                                                         >  2:551141/1‑247 (MQ=255)              ccAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGACGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGACTTTCTGTTCCCAACGGTAGTATTCAGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAACGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCgcaggc                                                                                                                                                                                                                                         >  1:18602/1‑248 (MQ=255)                 gTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGAcc                                                                                                                                                                                                                                    >  2:98375/1‑250 (MQ=255)                   aCGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGTTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGCCCaa                                                                                                                                                                                                                                  >  2:211807/1‑250 (MQ=255)                             cGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCTTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGGGGCTCCAGCCATAACCAAAGCCCAGCATTTTGAGCTGTTTTTTCATATCCGCGTTGTGGGC‑GTACGTCCATGGTGCCGGAG‑CGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCa                                                                                                                                                                                                                        <  2:487346/250‑1 (MQ=255)                             cGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCac                                                                                                                                                                                                                       >  2:269760/1‑250 (MQ=255)                              gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc                                                                                                                                                                                                                      >  2:384993/1‑251 (MQ=255)                                 cGGGCACCAGTTGACCGCAAAATTCTTCTAATATACCAGGCCTTTTTTA‑TACAGCTGGTTGAAGAATTTCTGTTCCAAACGGTAGTATCCCGGCGAACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCg                                                                                                                                                                                                                    <  1:397150/250‑1 (MQ=255)                                  gggCACCAGTGGCCCGCAAAAGTTTTTTAATAT‑CCCGGCCTTTTTTAATACAGCTCGGGAAAGAATTTCTGTTCCAACCGGTAGTATCCCGGGGTAAAGTTTGCCAGCTCGCGGCCCAAGTCATACCCAAAGCCCGGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTC‑GTACGTCCCCGGTGCGGGGG‑CGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAt                                                                                                                                                                                                                  <  1:302554/251‑1 (MQ=255)                                   ggCACCAGTGGACCGAAGAGGTCTTCTTATCTACCAGGCTTTTTTTA‑TACCGCGCGTTAAAGAATTTCTTTTCCCAACGGTAGTATTCCGGCGTACAGGGTGCCGGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCTTTTTGGGCTTGTTTTTCATATACGCGATGTTGCC‑GTACGCCCAGGGTGCCGGAG‑CGGTGTTTTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc                                                                                                                                                                                                                 <  2:324099/251‑1 (MQ=255)                                                       gTCTTCTTCTATACAAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGGTTTTGcc                                                                                                                                                                                             >  2:194336/1‑251 (MQ=255)                                                         cttcttATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGccc                                                                                                                                                                                            >  1:9058/1‑250 (MQ=255)                                                             ttATATACCAGGCTTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGAAGTATTCCGGCGTCAAGGTTGCCAGCTCGCGGCTCAAGTCAAAACCAAAGCCCAGCATTGTGAGCGGGTTTTTCATATACGGGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTCCAGGACGTTTTTGCCCAG‑Ca                                                                                                                                                                                       <  1:572956/250‑1 (MQ=255)                                                              tatataCCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CAt                                                                                                                                                                                      >  1:635997/1‑250 (MQ=255)                                                                 ataCCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGc                                                                                                                                                                                  >  1:63234/1‑251 (MQ=255)                                                                  taCCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCt                                                                                                                                                                                 >  1:425303/1‑251 (MQ=255)                                                                   aCCAGCCCTTTTTTA‑TACGGCTCGGTGAAAAATTTCTGTCCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCCCCAGTCATAACCAAAGCCCAGCATTTTGAGCGGTTTTTTCATATACGAGATGTTGTC‑GTGCGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTg                                                                                                                                                                                <  1:340266/251‑1 (MQ=255)                                                                        gCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGc                                                                                                                                                                           >  2:180527/1‑251 (MQ=255)                                                                                 a‑taCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTACCGGGCGATc                                                                                                                                                                   >  2:98431/1‑250 (MQ=255)                                                                                      cgcccGGTGAATAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTc                                                                                                                                                              <  1:116751/247‑1 (MQ=255)                                                                                       gcgcgttGAAGAATTTCGGTTCCCACCGGTAGTATTCCGGCGTACAGGTTCCCAGCTCGCGGCCCCATTCATACCCAAAGCCCATCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTCCGCCCACGGTGCCGTAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCa                                                                                                                                                             <  1:252481/245‑1 (MQ=255)                                                                                        cTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCAc                                                                                                                                                            >  2:61145/1‑251 (MQ=255)                                                                                          cGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                          >  1:68509/1‑251 (MQ=255)                                                                                           ggTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                          >  1:524625/1‑250 (MQ=255)                                                                                           ggTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGACGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACTCCTTCCGCAGGCAGACCACACGATTCACCGCCGATCGGCTGCAGGACGTTTTTGCCCAGACA‑ACGCTGGAATCTGGCGATCACGTAACCg                                                                                                                                                          >  2:360793/1‑250 (MQ=255)                                                                                            gTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCCCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATTTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGAt                                                                                                                                                        <  1:306148/251‑1 (MQ=255)                                                                                              gaagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                                                                                                                      >  2:630508/1‑251 (MQ=255)                                                                                                      tCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                                                                                                                               >  2:432864/1‑250 (MQ=255)                                                                                                      tCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                                                                                                                               <  1:584871/250‑1 (MQ=255)                                                                                                      tCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGGAGtt                                                                                                                                               >  2:384566/1‑250 (MQ=255)                                                                                                       ctgctCCCAACGGTAGGATTCCGGCGTACAGGTTACCAGCTCGCGGCTCCAGTCATAACTAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGAACAAACGCGACTCAGCCGAGCGGCTGCCGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGTGCGCTCACGACACCGATAGTGGAGTtac                                                                                                                                             >  2:616481/5‑251 (MQ=255)                                                                                                            cccAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTtacgtacg                                                                                                                                        >  1:207039/1‑251 (MQ=255)                                                                                                             ccAACGTTAGTATTCGGGGTTACGGTTTGCCGGCTCGCGGTCCCATTAATAACCAACGCCCAGTTTTTGGAGTGGGTTTTTCATATACGCGATGTTGGC‑GTACGTCCACGTTGCCGGGG‑CGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacgg                                                                                                                                       <  2:634600/251‑2 (MQ=255)                                                                                                             ccAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacgg                                                                                                                                       >  2:561717/1‑250 (MQ=255)                                                                                                                 cGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGGGTAGTTACGTACGTGGcc                                                                                                                                   >  1:366719/1‑251 (MQ=255)                                                                                                                   gtagtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGACATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATCTACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCCGGCAGACCAAACGCGTCCCAGCCGACCGGCTGCCGGACGCTTTTGCCCAG‑AATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTGTGTGGcca                                                                                                                                  >  2:327182/1‑249 (MQ=255)                                                                                                                      gtaTTCCGGCTTACAGGTAGCGAGCTCGCGTTCCCAGTCATACCCAAGCCCCTGCATTTTGAGTTGGTTTTCCATATACGCGTTGTTTTC‑TGACTCCCACGGGGCCTGAT‑CGGTGTTGTTTTTCACCGCCTTGCCTTCCGCATGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATAGTGTAGTTACGTACGTGGCCCAtgt                                                                                                                              <  1:530416/251‑1 (MQ=255)                                                                                                                      gtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtgt                                                                                                                              >  1:380589/1‑251 (MQ=255)                                                                                                                      gtaTTCCGGCGTACAGGGTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGACGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGAAGTTTTTGCCCAG‑AACACGCTGGTAGCGGGCGATCAAGTCACCGATGGTGTAGTTAAGGACGTGGcccgtgg                                                                                                                              >  2:19073/1‑247 (MQ=255)                                                                                                                        aTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATgtgt                                                                                                                            >  1:8061/1‑251 (MQ=255)                                                                                                                        aTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGAGGGTGTAGTTACGTACGTGGCCCATgtgt                                                                                                                            >  2:410017/1‑251 (MQ=255)                                                                                                                           ccGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGt                                                                                                                         >  1:381363/1‑251 (MQ=255)                                                                                                                            cGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                                                                                                                        >  2:383550/1‑251 (MQ=255)                                                                                                                                 tACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                                                                                                                    >  1:519101/1‑250 (MQ=255)                                                                                                                                  aCAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAg                                                                                                                  <  1:347772/251‑1 (MQ=255)                                                                                                                                    aGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGa                                                                                                                 >  1:514284/1‑250 (MQ=255)                                                                                                                                         cccggcccgggcttcaagcaatacccAACGCCAAGCTTTTTGACCGGGTTTTTAATATACGCGTTGTTGCC‑GTCCGCCCACGGTGCGGGGG‑GGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATa                                                                                                           <  2:212390/227‑1 (MQ=255)                                                                                                                                           cAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTAATATACGCGATGTTGTC‑GTACGTCCACGGGGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAgg                                                                                                         >  1:471995/1‑251 (MQ=255)                                                                                                                                            aGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAggg                                                                                                        >  1:286187/1‑251 (MQ=255)                                                                                                                                             gCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGa                                                                                                       <  1:630508/251‑1 (MQ=255)                                                                                                                                                            cATACCCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCa                                                                                         <  1:383550/250‑1 (MQ=255)                                                                                                                                                            aaTAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAg                                                                                        <  1:61145/250‑1 (MQ=255)                                                                                                                                                                  cAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGAC‑GTACGTCCACGTTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCACACCAAACGCGTCCCAACCGCTCGGCTGCAGAACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGACACCGATGCTGTAGTTACGTACGTGGCCCATGTGTAGTCCACCAGAAGGATAGGGAACCATAGACAGGCGGTAATat                                                                                  >  2:237720/1‑250 (MQ=255)                                                                                                                                                                    aaGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCCCGGTGCCGGAG‑CGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACtt                                                                                <  1:180527/251‑1 (MQ=255)                                                                                                                                                                        ccAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTctct                                                                            >  1:51150/1‑251 (MQ=255)                                                                                                                                                                         cggCATTTTGGGCTGGTTTTTCATATACTGGATGTTGTC‑GTACGCCCACGGTGCCGGGG‑CGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCtt                                                                           <  1:578080/249‑1 (MQ=255)                                                                                                                                                                          aGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCCCGGTGCCGGAG‑CGGGGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCttt                                                                          <  1:98375/251‑1 (MQ=255)                                                                                                                                                                             aTTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGct                                                                       >  1:202855/1‑251 (MQ=255)                                                                                                                                                                                   agttGGTTTTTCATATACGGGATGTTGTC‑GTCCGCCCACGGTGCCGGGC‑CGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTc                                                                 <  1:635005/248‑1 (MQ=255)                                                                                                                                                                                       gttttttCATATGCGCGATGTTGGC‑GTACGTCCCCGGTGCCGGGG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTc                                                              <  1:470666/248‑1 (MQ=255)                                                                                                                                                                                       ggTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCg                                                             >  1:129035/1‑251 (MQ=255)                                                                                                                                                                                         tttttCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGt                                                           >  2:5141/1‑251 (MQ=255)                                                                                                                                                                                           tttCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGtt                                                          >  1:229495/1‑250 (MQ=255)                                                                                                                                                                                           tttCATATA‑GCGGTGTTGTCCGTCCGTCCCCGGTGC‑GGAGGCGTTGTTGTTTTCCCCCGCCACGCCTTCCGCAGCCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTGTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGGAGCAGAGACAGGCAGTAATACCTCTCTTTGCTCTCGTCTTCGGtt                                                          <  2:216942/250‑1 (MQ=255)                                                                                                                                                                                               atatACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGAGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTc                                                     >  1:174239/1‑251 (MQ=255)                                                                                                                                                                                                 ataCGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCa                                                    >  2:630440/1‑250 (MQ=255)                                                                                                                                                                                                       gATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCATACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCCTGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgc                                             >  1:512162/1‑251 (MQ=255)                                                                                                                                                                                                        aTGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGGTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgcg                                            >  2:581563/1‑251 (MQ=255)                                                                                                                                                                                                             gtC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtct                                       >  1:24576/1‑251 (MQ=255)                                                                                                                                                                                                               c‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCTGTTACTTCAAATGTGCGCTtctc                                      >  2:601750/1‑250 (MQ=255)                                                                                                                                                                                                                     gTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccc                                 >  1:549996/1‑250 (MQ=255)                                                                                                                                                                                                                     gTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccc                                 >  1:610232/1‑250 (MQ=255)                                                                                                                                                                                                                       ccACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGACGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATccccat                              >  2:304766/1‑248 (MQ=255)                                                                                                                                                                                                                        ccgggTGCCGGTG‑CGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCATACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATg                             <  2:530407/248‑1 (MQ=255)                                                                                                                                                                                                                         aCGGTGCCGGAG‑CGGGGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGCCCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATg                             >  2:516237/1‑250 (MQ=255)                                                                                                                                                                                                                                  gAG‑CGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACt                   <  1:211807/251‑1 (MQ=255)                                                                                                                                                                                                                                             gttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCt          >  1:347994/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ttctccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAAGGTGCGCTTCTCAACCCAATGAAGCTGTACTTTGGATTCTAtct      <  2:629427/246‑1 (MQ=255)                                                                                                                                                                                                                                                  cccccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtctc     <  2:347994/247‑1 (MQ=255)                                                                                                                                                                                                                                                    cccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTc  <  2:411061/250‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      ACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTA‑TACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTC‑GTACGTCCACGGTGCCGGAG‑CGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674272‑674762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑