Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	98.8%	299.5 / ‑4.6	81	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (34/46);  total (35/46)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGC‑CAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560207‑3560687                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           cttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGcgc                                                                                                                                                                                                                                           >  2:442806/1‑249 (MQ=255) cttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGcgc                                                                                                                                                                                                                                           >  1:245719/1‑249 (MQ=255)   tcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTCCCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                             <  1:599670/247‑1 (MQ=255)   tcttTTACCATCAACGTGTTAGAAACGTTGAGATTGTGGGTG‑ACAATGCGCAAATTGCTGTGATGGAGCAGGGCTTGCG‑CTACCG‑CTCCCGGGGTGG‑TGCCGGTATCAATAAA‑C‑AGCGTCGAGCCATTGGGT‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                             <  1:227831/247‑1 (MQ=255)     ttttACCATCCACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACATTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGCGCCATTGGGG‑CT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGTCCTTGCGATCGTGCCACGGCGCGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                        >  2:455807/1‑245 (MQ=255)     ttttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                        >  1:631615/1‑245 (MQ=255)        tACCATAAACGGGTTAGCAGCGTTGAGATTGTGGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGGGTGCG‑CTACCG‑CTCCGGGGGGGG‑GGCCGATATCGATAAA‑C‑GGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATc                                                                                                                                                                                                                                    <  1:506083/250‑1 (MQ=255)        cccccacAACTTGTTGGCAAGGTGGAGATTGT‑GGTGGACAATGCGCAAATTGTTGTGATTGAGCGGTTGGTGCG‑CTCCCG‑TTTCCGGCGTGG‑TGCCGATCTCG‑TAAAGT‑AGCGTCGGGCC‑TTTGGG‑ATGTT‑G‑CTCC‑GCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACGGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCa                                                                                                                                                                                                                                   <  1:239013/245‑1 (MQ=255)          ccATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGAGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAgg                                                                                                                                                                                                                                 >  1:523576/1‑251 (MQ=255)           cATCAACGTGTTAACAACGGTGGGATTGTTGGTG‑ACAATGCCCAAATTGCGGTGTTGGGGCAGTGGGTGCG‑CTACCG‑CTCCCGGCGTGG‑GGCCGATATCGATAAC‑C‑AGCGTCGAGCCATTGGGG‑TT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCGGCTCCGCACCGCCATGATGGCGCAG‑GATCAGGt                                                                                                                                                                                                                                <  1:181251/251‑1 (MQ=255)             tcaccGTGTTAGAAACGTTGAGATTGTGGGTG‑ACAATGCGAAAATTGCTGTGATTGAGAAGGGGGTGCG‑TTACCG‑CTCCCGGCGTGT‑TGCCGATATCGTTAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGG‑AAGGCAGCGCCGCTCCGCCATGATGGCGCAG‑GATCAGGtt                                                                                                                                                                                                                               <  1:457152/246‑1 (MQ=255)             caaaCGTGTAAGAAACTTTGAGATTGTGGGGG‑ACAATGCGCAATTGGCTTTGATTGAGCAGGGCGTGCG‑TTCCCG‑CTTCCGGCTGGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCGTTGGGG‑TT‑TT‑G‑CTCC‑GCCACTTTGCGGCCGATGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGACCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGttt                                                                                                                                                                                                                              <  1:135359/249‑1 (MQ=255)              cAACGTTTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTGCCGGGGGGG‑TGCGGATATCGATAAA‑C‑GGCGTGGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGtttt                                                                                                                                                                                                                             <  1:246849/251‑1 (MQ=255)              cAACGTGTTAGCAACGATGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGCGCAGTGTGTGCG‑CTACCG‑CTTCCGGCGAGG‑TGCCGTTATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGCGGCCTTGCGATAGTGCCACGGCGTGTTTACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGtttt                                                                                                                                                                                                                             >  2:493424/1‑251 (MQ=255)               aaCGGGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTAACG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCAACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTg                                                                                                                                                                                                                            >  1:289609/1‑251 (MQ=255)                aCGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGc                                                                                                                                                                                                                           >  2:148283/1‑251 (MQ=255)                 cGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCCGGTTTTGcc                                                                                                                                                                                                                          >  2:566586/1‑250 (MQ=255)                  gtgtTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTc                                                                                                                                                                                                                         >  1:623595/1‑251 (MQ=255)                          actgtggggatggttgGTG‑ACAATGCTCAAATTGCGGTGATTGGGCGGTGCG‑GCGTCT‑CCG‑CTTCCGGGGGGGGTGCAGATAGCAAAAAA‑C‑GGCTGCGGGCAATGGGGT‑GT‑GT‑G‑CTCC‑GCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                 <  2:613339/239‑1 (MQ=255)                                  gaTTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑gg                                                                                                                                                                                                        >  1:538414/1‑251 (MQ=255)                                     tgttgGGG‑ACAATGCGCAAATTGTTGTGATTGAGCGGTGCGTGCG‑CTCCCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTc                                                                                                                                                                                                      <  1:434811/250‑1 (MQ=255)                                                 aTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCA‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCGGCGTCGCACCGCCATGATGACGCAG‑GAACAGGTTTTGCCCCGCCAGCTC‑TTGAAGGTTGCGGCGAATAgg                                                                                                                                                                                          >  2:52522/1‑250 (MQ=255)                                                    ggaaattggtggggATTGAGCGGGGCGTGCG‑CT‑CCGTCTTCGGGCGGGG‑TGCCGTTAGCGAAAAA‑C‑AGCGCGGACCCGTTGGGT‑AT‑TT‑G‑CCCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTg                                                                                                                                                                                       <  2:631615/238‑1 (MQ=255)                                                     gcAAATTGCTGTGATTGAGCAGTGCGAGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑A‑AGCGTCGATCCATTGAGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGCTCTGGGTGACCTTGCGATCGTGCCACGGCGTGTTCACCGCACTGG‑ACGGCAGCGCCCCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCACCTCATAGA‑GGTCGGGCCGAACAGTCTGc                                                                                                                                                                                      >  2:136468/1‑251 (MQ=255)                                                       aaatggatgtgatggggctgggcgtgcg‑CTCCCC‑CTTCCGGGGTGG‑TGCCGATCT‑GAAAAA‑CCAGCGGCG‑GCCCTTGGGGGTT‑TT‑G‑CCCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                    <  2:105391/230‑1 (MQ=255)                                                         aTTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCATCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTGTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                  >  1:4545/1‑251 (MQ=255)                                                            gCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                                >  1:155264/1‑250 (MQ=255)                                                            gCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCAGATATCGATAAA‑C‑AGCGTAGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGACTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACAGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGGCTGCCGGCTgc                                                                                                                                                                               >  2:523019/1‑250 (MQ=255)                                                               gggtttaaGCAGTGCGCGCG‑CTACCT‑CTTCCGGCGTGG‑TGCCGATATCGATCCA‑C‑AGCGGCAACCCATTGGGG‑CT‑TT‑G‑CACC‑GCCACTTTGCAGGCGATGCGCTCTTTTGCTTGGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAAGTGG‑AAGGCAGCGCCGCACCGCCATGATGGC‑GTGCGATCAGGTGTTGCTCTGCAAGCTCATTGG‑GGTCGCGGCGTAAAGGCTGCgggttggcgg                                                                                                                                                                            >  2:39131/8‑244 (MQ=255)                                                                 gATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                            >  1:488400/1‑249 (MQ=255)                                                                   ttGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                                                                                                                         >  1:325378/1‑250 (MQ=255)                                                                      aGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                     >  2:91584/1‑251 (MQ=255)                                                                       gCAGTGCGTGCG‑CTCCCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAC‑C‑AGCTTCGAGCCATTGGGG‑TT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                                    <  1:21459/251‑1 (MQ=255)                                                                         aGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                  >  2:252103/1‑251 (MQ=255)                                                                            gcgtgcg‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                                                                                                               >  1:249962/1‑251 (MQ=255)                                                                              gtgcg‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTTA‑GGTCGCGGCGACTAGTCTGCGGGCTGACGGAGAAATGctctccc                                                                                                                                                              >  2:545602/1‑247 (MQ=255)                                                                                      cccG‑CTTCCGGCGGGG‑TGCCGTGATCGAAAAA‑C‑AGCGTCGATCCATTGGGG‑AT‑TT‑T‑CCCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                       <  1:401086/249‑1 (MQ=255)                                                                                      aCCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                      >  1:461505/1‑251 (MQ=255)                                                                                               cGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                                                                                                                               >  1:164831/1‑250 (MQ=255)                                                                                               cGGCGTGG‑GCCCTATACCGAAAAA‑C‑GGCGTCGGGCCGTGGGGG‑TT‑TT‑G‑CCCC‑GCCACGTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGTTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                              <  1:513274/251‑1 (MQ=255)                                                                                                ggCGTGG‑CGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GACACTTTGCGGGCGATGCGCTCTTTTTGTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                                                                                                                             >  1:554199/1‑251 (MQ=255)                                                                                                         gccaatatCGAAAAA‑C‑AGCGCCGAGCC‑TTGGGG‑GT‑TT‑GGCTCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                     <  1:491498/247‑1 (MQ=255)                                                                                                         gCCGATATCGATAAA‑C‑AGCGTCGAGCAATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                     >  1:380433/1‑251 (MQ=255)                                                                                                            gATATGGATAAC‑C‑TGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                                  <  1:109294/251‑1 (MQ=255)                                                                                                            ccgattacgaaaaatcggttggggCCATTGGGG‑AT‑TTTG‑CGCC‑GCTGTTTTGCGGGGGAGGCGCTCTTTTTCAGCGTTCGGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                     <  2:600918/228‑1 (MQ=255)                                                                                                                   cgaaaaaggcgttcggtctttggggg‑AT‑TT‑C‑CTCC‑GCCACTTGGCGGGCGATGCGCTCTTTTTCTGCGTTCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                              <  2:581254/228‑1 (MQ=255)                                                                                                                       aaaccaggttcggcctttGGGG‑TT‑TT‑G‑CCCCCGCCACTTTGCGGGCGATGCGCTCTTTTCCTTCGGTTGGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                          <  2:623595/235‑1 (MQ=255)                                                                                                                                    ccttggggg‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                                                                                                            <  1:359551/246‑1 (MQ=255)                                                                                                                                         gggg‑TT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGTTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTTTTAACCGAACTGG‑AAGGCGGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                       <  1:214197/251‑1 (MQ=255)                                                                                                                                                  t‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                >  1:479844/1‑251 (MQ=255)                                                                                                                                                    tccTCC‑GCCACTTTGCGGGCGGTGCGCTCTTTTGCTTGGTTCTGGGTGCCCTTGGGGTGGTGCCCCGGGGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                 <  2:537972/248‑1 (MQ=255)                                                                                                                                                       ccc‑GCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTgtgt                                                                                             <  2:241274/250‑1 (MQ=255)                                                                                                                                                         c‑GCCTCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACGGTTGTGACGTTGtgttt                                                                                           <  1:128405/251‑1 (MQ=255)                                                                                                                                                             catttggCGGGGGATGCGCTCTTTTGCTTCGGTGTGGGGGCCCTTGCGATGGTGCCCCGGCGTGTTAACCGAACGGG‑AAGGCCGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                        <  2:210760/245‑1 (MQ=255)                                                                                                                                                                  ggCGGGCGAGGCGCTTTTTTTCTTCGGGGTGGGTGCCCTCGCGATCGGGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAt                                                                                    <  2:69696/249‑1 (MQ=255)                                                                                                                                                                   gcggcctaTGCGCTCTTTTCCTGCGGTGTGGGGGGCCTTGGGACCGGGCCAGGGCGTGTTAACCGAACTGG‑AAGGCAGCCCCCCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                  <  2:325378/244‑1 (MQ=255)                                                                                                                                                                        cGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                              >  1:150704/1‑250 (MQ=255)                                                                                                                                                                         gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTc                                                                                                                                                                                                      >  1:276098/1‑129 (MQ=255)                                                                                                                                                                         gATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTc                                                                                                                                                                                                      <  2:276098/129‑1 (MQ=255)                                                                                                                                                                           tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                         >  2:182918/1‑220 (MQ=255)                                                                                                                                                                           tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                         <  1:182918/220‑1 (MQ=255)                                                                                                                                                                             ggctttttttCGTCGGTCGGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGACCTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                        <  2:276342/246‑1 (MQ=255)                                                                                                                                                                             cgcTCTTTTTCTGCGGGCTGGGGGGCCTGGCGATCGCGCCACGGCGTTTAAACCGAACGGG‑AAGGCAGCGCCGCCCCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                        <  2:142832/251‑1 (MQ=255)                                                                                                                                                                                   ttttCTTCGGCCTGGGGGTCCTTGCGATCGTGCCCCGGTGGTTTAACCGAACTGA‑AAGCCAGCGCCGCTCCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAat                                                                   <  2:501185/250‑1 (MQ=255)                                                                                                                                                                                   ttttCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAat                                                                   <  2:155264/250‑1 (MQ=255)                                                                                                                                                                                   tttgcttgggTCGGGGGGGCCTGGCGATCGGGCCACGGGGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                                     <  2:97489/240‑1 (MQ=255)                                                                                                                                                                                     ttcttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                >  2:408077/1‑251 (MQ=255)                                                                                                                                                                                       gttcGGGCTGGTTGGCCTGGCGACCGTGCCGCGGCGTGGTAACCGACCTGG‑AAGGCAGCGCCTCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                               <  2:347350/249‑1 (MQ=255)                                                                                                                                                                                         tcGGTGTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                             <  2:12592/250‑1 (MQ=255)                                                                                                                                                                                         tcGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                             <  1:511086/250‑1 (MQ=255)                                                                                                                                                                                         gcggtctgtggggcttggCGATCGTGCCACGGCGTGTTGACC‑ACCTGGGAGGGCCGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                             <  2:559374/233‑1 (MQ=255)                                                                                                                                                                                                gggTGGCTTGGCGATCGTGCCGCGGGTTGTTAACCGAACTGA‑ACGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGATAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                     <  2:214173/251‑1 (MQ=255)                                                                                                                                                                                                gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtttt                                                       <  1:252103/249‑1 (MQ=255)                                                                                                                                                                                                   gggCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                  <  1:165444/250‑1 (MQ=255)                                                                                                                                                                                                      cctggcgaccttGCCACGGCGGGTTAACCGACCTGG‑AAGGCGGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                               <  2:245719/240‑1 (MQ=255)                                                                                                                                                                                                            gATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACAGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTCTTTTCGTTTTCGCGCATTGAGCg                                          >  1:36366/1‑250 (MQ=255)                                                                                                                                                                                                                  gCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATACTACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGCATTATTTTCGTTTTCGCGCATTGAGCGAATcaa                                    >  2:245889/1‑250 (MQ=255)                                                                                                                                                                                                                   ccACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGGGCATTGAGCGAATcaac                                   >  2:390968/1‑250 (MQ=255)                                                                                                                                                                                                                    cACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                  >  1:368669/1‑250 (MQ=255)                                                                                                                                                                                                                       ggCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg                              <  1:427639/251‑1 (MQ=255)                                                                                                                                                                                                                          gtgtTAACCGAACTGG‑AAGGCAGCGCCGCAACGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGACTTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                            >  2:397456/1‑250 (MQ=255)                                                                                                                                                                                                                           ggtTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt                          <  1:245889/250‑1 (MQ=255)                                                                                                                                                                                                                                cccaaaCTGG‑AGGGCAGCGCCCCACCGCCATGAGGGCGCAG‑GATTAGGTTTTGCTCCGCCTGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa                     <  2:340219/247‑1 (MQ=255)                                                                                                                                                                                                                                  cGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAACTCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa                    >  1:455153/1‑250 (MQ=255)                                                                                                                                                                                                                                              gCAGCGCCGCACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTCTTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa         >  2:317507/1‑250 (MQ=255)                                                                                                                                                                                                                                              gCAGCCCCGTACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCTCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTaa        <  1:87753/251‑1 (MQ=255)                                                                                                                                                                                                                                                    ccgcACCGCCATGATGGCGCAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc  <  1:148283/251‑1 (MQ=255)                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTG‑ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCG‑CTACCG‑CTTCCGGCGTGG‑TGCCGATATCGATAAA‑C‑AGCGTCGAGCCATTGGGG‑AT‑TT‑G‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGG‑AAGGCAGCGCCGCACCGCCATGATGGC‑CAG‑GATCAGGTTTTGCTCCGCCAGCTCATTGA‑GGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560207‑3560687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑