Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,310	C→T	A110V (GCG→GTG)	lhgD →	L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,310	0	C	T	100.0%	262.2 / NA	69	A110V (GCG→GTG)	lhgD	L‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/41);  total (28/41)

CGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGG  >  NC_000913/2790063‑2790543                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          cGCATTGCGTTGATGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCATAAAAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAATGCGCAGTTTTGCCTGGCGGGAAACCGCGCCAACAAAGACTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGCTGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGCAGCGCGCCTTATGGGAACGCGCAggggc                                                                                                                                                                                                                                        >  2:34035/1‑251 (MQ=255)        cGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                                                                                                                                                                                 <  1:167380/251‑1 (MQ=255)         gTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTa                                                                                                                                                                                                                                <  1:360231/251‑1 (MQ=255)           ttcggaaaaaaGAGCCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTAt                                                                                                                                                                                                                               <  2:414421/244‑1 (MQ=255)            gCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATc                                                                                                                                                                                                                              <  1:42865/250‑1 (MQ=255)                          ccGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                                                                                                                                                                               >  2:522010/1‑251 (MQ=255)                           cgcccccgcccgtccccgggcGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTTTTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTa                                                                                                                                                                                                              <  1:325659/231‑1 (MQ=255)                                       gcACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAc                                                                                                                                                                                                  <  1:76165/250‑1 (MQ=255)                                             gACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcg                                                                                                                                                                                            <  2:280385/251‑1 (MQ=255)                                               gggggcacaACAGCGGCGTGATCCATGCCGGGGTCTATTACGCGTCCGGGAGCCTGAAGGCGCAGTTTTGCCGAGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGGTGCCACGTCCGATCTCGAAATGGAACGGATACGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAAGGGCTTAACGCCGACGAACTgcgcgag                                                                                                                                                                                          <  2:547199/246‑1 (MQ=255)                                               cGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgag                                                                                                                                                                                          <  1:485780/251‑1 (MQ=255)                                                      caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                                                                                                                                                                                   <  1:17123/251‑1 (MQ=255)                                                      caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                                                                                                                                                                                   <  1:43768/251‑1 (MQ=255)                                                       aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAcc                                                                                                                                                                                  <  1:129924/251‑1 (MQ=255)                                                                 tGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAcc                                                                                                                                                                        >  1:52246/1‑251 (MQ=255)                                                                     ccATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGc                                                                                                                                                                    <  1:437472/251‑1 (MQ=255)                                                                       aTGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTc                                                                                                                                                                  >  1:481129/1‑251 (MQ=255)                                                                         gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcg                                                                                                                                                                 <  1:316195/250‑1 (MQ=255)                                                                          ccGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcggc                                                                                                                                                               >  1:7637/1‑251 (MQ=255)                                                                              ggTCTATTACACGTCCGGCAGCCTGAAGGAGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGACCGCGAACAGAATATCACAGGGCTCggcggc                                                                                                                                                            >  2:292787/1‑250 (MQ=255)                                                                                 ctattacacgtccggcctccctcaGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTTCTTCCCTCTTCTTTTTTCCCCTTCCCCTCCCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAttt                                                                                                                                                        >  1:294242/24‑251 (MQ=255)                                                                                  tATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtttt                                                                                                                                                       >  1:227086/1‑251 (MQ=255)                                                                                   attccacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAttttt                                                                                                                                                      <  2:430389/247‑1 (MQ=255)                                                                                            ccGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGttc                                                                                                                                             <  2:219807/251‑3 (MQ=255)                                                                                            ccGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTcc                                                                                                                                             >  2:25411/1‑251 (MQ=255)                                                                                               gCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCggcccgc                                                                                                                                          >  2:281677/1‑245 (MQ=255)                                                                                                cAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCAGCATTTTTGTGCCGTCCAGCg                                                                                                                                         <  1:441240/251‑1 (MQ=255)                                                                                                       aagtcgcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCAgtgt                                                                                                                                  <  2:65623/247‑1 (MQ=255)                                                                                                            gcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGAAAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCt                                                                                                                             <  2:382030/251‑1 (MQ=255)                                                                                                                    gCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGAc                                                                                                                     <  1:369246/251‑1 (MQ=255)                                                                                                                         gCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGAc                                                                                                                <  2:52246/251‑1 (MQ=255)                                                                                                                          ggggAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGAcg                                                                                                               <  2:263550/250‑1 (MQ=255)                                                                                                                            ggAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGAcggc                                                                                                             <  1:275715/251‑1 (MQ=255)                                                                                                                             gAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCCTTTTTGTGCCGTCCAGCGGCATTGTCAGCCACCGCGACGTGAcggcg                                                                                                            >  2:525291/1‑251 (MQ=255)                                                                                                                               aaCCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACggcggc                                                                                                          <  2:120184/251‑1 (MQ=255)                                                                                                                                     gcCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTCCCGCGACGGGACGGCGGCGATggg                                                                                                    >  2:329035/1‑250 (MQ=255)                                                                                                                                       caccaAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCa                                                                                                   >  2:552662/1‑250 (MQ=255)                                                                                                                                        accaAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTATGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCaa                                                                                                  >  1:473155/1‑250 (MQ=255)                                                                                                                                                cTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGCCGTGACGGCGGAGATGGCAAAAATCTtcc                                                                                         >  2:137018/1‑251 (MQ=255)                                                                                                                                                cTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtcc                                                                                         >  2:505587/1‑251 (MQ=255)                                                                                                                                                   ttGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagt                                                                                      >  1:103816/1‑251 (MQ=255)                                                                                                                                                   ttGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagt                                                                                      >  2:545981/1‑251 (MQ=255)                                                                                                                                                    tGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtcgagt                                                                                      <  1:144828/250‑1 (MQ=255)                                                                                                                                                     gCGATCAAAACGGCATGCGCGACTACATCTGCGGCAAGAGGCGGGTTGCCCCGTCCGATCTCGAAATGGAACGGATGCTCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGATTATCACCGGGCTCGGCGTCATTTTTGTGCCGTCCAGCGGCATTGTCATCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagtcc                                                                                    <  2:461803/251‑1 (MQ=255)                                                                                                                                                      cGATCAAAACGGCATTCGCTACGACAACTGCGCCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACGGTGGCGAACGGCATCGAGCGCGAATGGCTTAACGCCGCCGAACTGCGCCAGCGCGAACCGAATATCACCGGGCTCGGCGGCCTTTTTGTGCCGTCCAGCGGCATTGTCCGCTACCGCGACGTGACGGCGGCGCTGGCAAAAATCTtccagtccc                                                                                   >  2:482262/1‑250 (MQ=255)                                                                                                                                                       gATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGCCGTGACGGCGGCGATGGCAAAAATCTtccagtcc                                                                                    >  1:73234/1‑249 (MQ=255)                                                                                                                                                       gATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagtccag                                                                                  <  1:522010/251‑1 (MQ=255)                                                                                                                                                          cAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAgg                                                                               >  1:474893/1‑251 (MQ=255)                                                                                                                                                                ggCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGGTGGCAAAAATCTTCCAGTCCAGcggcgggtg                                                                         >  2:430309/1‑248 (MQ=255)                                                                                                                                                                     tCGCTACGACAGCTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACATTGGCGAGCGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGTCGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAAtt                                                                     <  2:301486/250‑1 (MQ=255)                                                                                                                                                                      cGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTa                                                                    <  1:49520/250‑1 (MQ=255)                                                                                                                                                                       gCTACGACAACTGCGGCAAGATGCTTGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGATCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCAGCATTGTCAGCTACCGCGACGTGACAGCGGCGATGGCAAAAATCTTCCATTCCAGAGGCGGTGAAATTATc                                                                  <  2:79770/251‑1 (MQ=255)                                                                                                                                                                       gCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGAGCTGGCAAAAATCTTCCAGTCCAGAGGCgggggaaatttt                                                                  >  2:325540/1‑241 (MQ=255)                                                                                                                                                                          aCGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCt                                                                 <  1:461622/249‑1 (MQ=255)                                                                                                                                                                           cGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTgtacatatctatt                                                              >  1:436392/1‑239 (MQ=255)                                                                                                                                                                            gACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATaa                                                             <  1:270776/251‑1 (MQ=255)                                                                                                                                                                            gACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATaa                                                             <  2:165600/251‑1 (MQ=255)                                                                                                                                                                             aCAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGaaaatttctttaa                                                             >  1:498878/1‑240 (MQ=255)                                                                                                                                                                              cAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGTTGAAATTATCTATAACg                                                           <  1:362684/251‑1 (MQ=255)                                                                                                                                                                                  tGCGGCAAGATGCTGGGTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCgggggaaatatctgtaaaccccc                                                       >  2:189265/1‑230 (MQ=255)                                                                                                                                                                                           ctGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCCGAGGCGGGGAAATTATCTATTACGCCGAAGTTAGCgg                                              >  2:291738/2‑251 (MQ=255)                                                                                                                                                                                                ggTTGCCAAGTCCGATCACAAAATGGAGCGGATGCGCGCCTTCTGGGAACGCACAGTGGCGAACGGTGTCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCTTTTTTGTGCCCCCCCGCGGCATTGTCAGCTACCGCGGCGTGGCGGCGGCGATGGCCAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCt                                           <  2:516064/249‑1 (MQ=255)                                                                                                                                                                                                    gCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCCCCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATg                                      <  2:73234/250‑1 (MQ=255)                                                                                                                                                                                                    gCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGGGGAATTATTTATAAAACCGCAGTCAGCGGGGTTAATg                                      >  1:530319/1‑250 (MQ=255)                                                                                                                                                                                                         gTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATa                                <  1:322658/251‑1 (MQ=255)                                                                                                                                                                                                                          aaCGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGAGGAATTTTTTTTAAAGCCGAAGGCAGAGGGCGTACTGAggcaaaaaacagggtggggaa                >  1:246438/1‑230 (MQ=255)                                                                                                                                                                                                                           aCGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATAc              <  1:400508/251‑1 (MQ=255)                                                                                                                                                                                                                             ggATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGt            <  2:265057/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcgcgcCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGaa                                                      <  2:246000/205‑1 (MQ=255)                                                                                                                                                                                                                                         tATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAgg  <  2:108190/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          CGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGG  >  NC_000913/2790063‑2790543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑