Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,153	C→T	P58S (CCC→TCC)	lhgD →	L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,153	0	C	T	100.0%	267.4 / NA	74	P58S (CCC→TCC)	lhgD	L‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/35);  total (39/35)

AGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTT  >  NC_000913/2789913‑2790395                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   aacgaaaagctctattgtttacgttgggcctaaacgcaggaagcctccccGCGCAAAGGAATTGA‑GCGGATGTATGATTCTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCt                                                                                                                                                                                                                                          <  1:76389‑M2/201‑1 (MQ=255)    gaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGa                                                                                                                                                                                                                                        >  1:550874‑M2/48‑250 (MQ=255)        agctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGAATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGAAGCCCGCATTGCGATGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGAGGGATCCATGCCGGGGGCTATTACACGTCCCGCAGCCTGaagggg                                                                                                                                                                                                                                   >  2:343567‑M2/44‑249 (MQ=255)            ctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGCATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGAACGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGt                                                                                                                                                                                                                               >  2:400508‑M2/40‑251 (MQ=255)              attgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACCACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGttt                                                                                                                                                                                                                             >  1:134186‑M2/38‑251 (MQ=255)                      cgttgggcctaaacgcagggagaccccccGCGCAAAGGAATTGG‑GCGGATGTATGATTTTGTGATTATGGGCGGCGGCATCATCGGCATGT‑CGACCGCCAGGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGCCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGc                                                                                                                                                                                                                     <  2:203919‑M2/222‑1 (MQ=255)                           ggcctaaacgcggggagcctccccGGGCAAAGGAATTGA‑GCGGATGTATGATTGTGTGATTATTGGCGTCGGCATCAGCGTCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGGGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGaa                                                                                                                                                                                                                <  2:231840‑M2/227‑1 (MQ=255)                               taaacgcagggagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACcg                                                                                                                                                                                                            >  1:354333‑M2/21‑251 (MQ=255)                                aaacgcagggagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACcgc                                                                                                                                                                                                           >  1:310521‑M2/20‑251 (MQ=255)                                aaacgcagggagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGAGATTCTTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCCTGCCGGGGGCTATTCAACGTCCGGCCGCCTGAAGGCGCAGTTTTGCCGGGGGGGAaacccc                                                                                                                                                                                                           >  2:325659‑M2/20‑249 (MQ=255)                                   cgcagggagactccccCCGCAAGGGATTTGA‑GCGGTTTGATGATTTTGGGATTATTGGGGGCGGCATTATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTTTATCCGGACGCCGGCATTGCGTTGCTGGAAAAGGAGTCCGCCCCTGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGcc                                                                                                                                                                                                        <  2:417808‑M2/235‑1 (MQ=255)                                     aagggagaccccccGCGCAAAGGAA‑TGAGGCGGATGTATGATTTGGTGATTTTTGTCGGCGGCACCACCGGCA‑GTGCGACCGCCAGGCAACTGATTGATGTCTATCCGGACGCCGGCAGTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCAAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccac                                                                                                                                                                                                      <  2:59274‑M2/237‑1 (MQ=255)                                         gagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCaaa                                                                                                                                                                                                  <  1:323274‑M2/241‑1 (MQ=255)                                         gagactccccGCGCAAAGGAATTGA‑GCGGATGTATGATTTGGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCaaa                                                                                                                                                                                                  <  1:519553‑M2/241‑1 (MQ=255)                                           gactccccGCGCAAAGGAATGGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGCCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGc                                                                                                                                                                                                <  1:528699‑M2/243‑1 (MQ=255)                                                cccGCGCAAAGGAATTGG‑GGGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCAGGT‑CGACCGCCAGGCAACTGATTGATTTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCtttt                                                                                                                                                                                           <  2:462427‑M2/248‑1 (MQ=255)                                                cccGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTATATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACCTCCGGCAGCCTGAAAGCGCAGGTTTCCCTGGCGGGAAACCGCACCACCCAAGCCtttt                                                                                                                                                                                           >  2:111859‑M2/4‑251 (MQ=255)                                                     gcAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGAt                                                                                                                                                                                      >  1:223970/1‑251 (MQ=255)                                                       aaaGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGTAAAAGAGTCCGCCCCGGCCTGTCACCAGACCGGCCACAACAGCGGCGCGATCCATGCCGGGGTCTATTACACGTACGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATc                                                                                                                                                                                     >  1:234849/1‑250 (MQ=255)                                                             aTTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGG‑CGAACGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGACACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCCGCAGCCTGACGGCTCAGTTTTGCCTGGCGGGAAACCGCGCCAACCAAGCCCTTTGGGAACAAAACgg                                                                                                                                                                              >  2:347685/1‑251 (MQ=255)                                                                   gCGGATGTATGATTTGGTGATTATTGGCGGCGGCTTCATGGGCATGT‑CGTCCGCCATGCACCTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTc                                                                                                                                                                         <  1:226252/251‑1 (MQ=255)                                                                         gaaggATTTTGTGTTTATGGGCGGCGGCAGCAGCGGCATGT‑CGCCCGCCAGGCAACTGATGGATGTCTACCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACAAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTAc                                                                                                                                                                    <  2:36285/246‑1 (MQ=255)                                                                         gTATTATTTTGGGATTATTGGGGGCGGCATCATCGGCATGT‑CGCCCGCCATGCACCTGATTGATGCCTATCCGGCCGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTAc                                                                                                                                                                    <  2:453421/250‑1 (MQ=255)                                                                           aTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAACAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCCCCACAGCCTTTTGCGATCAAAAAGGCATTCGCTACGAc                                                                                                                                                                 >  2:486136/1‑251 (MQ=255)                                                                           aTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGAc                                                                                                                                                                 >  2:43768/1‑251 (MQ=255)                                                                           aTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGAc                                                                                                                                                                 >  2:205532/1‑251 (MQ=255)                                                                               ttttGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAAcc                                                                                                                                                             >  1:438823/1‑250 (MQ=255)                                                                                 ttGTGTTTATTGGCGGCGGCATCATCGGCATTT‑CGCCCGACATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGGGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCCAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACATCTg                                                                                                                                                            <  2:328824/250‑1 (MQ=255)                                                                                         atgggcggcggcATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGa                                                                                                                                                    <  1:24045/247‑1 (MQ=255)                                                                                               ggcggcATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGCTGCCGGt                                                                                                                                             >  2:441240/1‑251 (MQ=255)                                                                                                  ggcATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGCTGCTGGTTGc                                                                                                                                          >  1:461803/1‑251 (MQ=255)                                                                                                      tcatcGGCATGT‑CGACCGCCATGCAACTGACTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCTGGGTCTATTACACGTCCGGCAGACTGCAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCAACAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCACGATGCTGGCTGCCAcc                                                                                                                                      >  2:415567/1‑250 (MQ=255)                                                                                                         tcGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTcc                                                                                                                                   >  1:219807/1‑251 (MQ=255)                                                                                                              aTGT‑CGACCGCCATGCAACTGATTGATTTTTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtct                                                                                                                              <  1:197898/251‑1 (MQ=255)                                                                                                              aTGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtct                                                                                                                              >  1:265057/1‑251 (MQ=255)                                                                                                               atggcGACCGCCATGCAACTGATTGATGTCTTTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtct                                                                                                                              <  2:47739/247‑1 (MQ=255)                                                                                                                     aCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAAt                                                                                                                        >  1:280385/1‑251 (MQ=255)                                                                                                                        gCCATGCAACTGATTGATGTCTATCCGGACGTCCGCATTGCGTTGCTGGAAAAAGCGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTTGCGGGAAACCGCGCCACCAAAGCCTTTTACGATCAAAACGGCATTCGCTACGACAACTGCGCCAAGATGCTGGGTGCCACGTCCCATCTCGAAATgg                                                                                                                      >  2:123842/1‑250 (MQ=255)                                                                                                                         ccATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGaa                                                                                                                    <  1:148467/251‑1 (MQ=255)                                                                                                                             gCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACgg                                                                                                                 >  1:75028/1‑250 (MQ=255)                                                                                                                                  tgattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGCTgcgc                                                                                                           >  2:275715/1‑251 (MQ=255)                                                                                                                                   gattgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcgcg                                                                                                          >  1:120184/1‑251 (MQ=255)                                                                                                                                        atGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCtt                                                                                                      >  1:314251/1‑250 (MQ=255)                                                                                                                                              aTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGa                                                                                                >  1:217572/1‑250 (MQ=255)                                                                                                                                               cccGGACCCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAAc                                                                                              <  2:134186/250‑1 (MQ=255)                                                                                                                                                ccGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACg                                                                                             >  1:165600/1‑251 (MQ=255)                                                                                                                                                  ggACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGACGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAAAGGATGCGCGCCTTATGGGAACGc                                                                                            >  1:395129/1‑250 (MQ=255)                                                                                                                                                   cccccccGCATTTCGTTGCTGGAAAAAGAGTCCCCCCCGGCCTGTCCCCGGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcac                                                                                          <  2:419086/247‑1 (MQ=255)                                                                                                                                                    aCGCCCGCATTGCGTTGCTGGCAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcaa                                                                                          >  2:461622/1‑249 (MQ=255)                                                                                                                                                      gCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAgt                                                                                       >  1:368487/1‑250 (MQ=255)                                                                                                                                                         cGCATTGCGTTGATGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCATAAAAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAATGCGCAGTTTTGCCTGGCGGGAAACCGCGCCAACAAAGACTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGCTGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGCAGCGCGCCTTATGGGAACGCGCAggggc                                                                                    >  2:34035/1‑251 (MQ=255)                                                                                                                                                                cGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                             <  1:167380/251‑1 (MQ=255)                                                                                                                                                                 gTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTa                                                                            <  1:360231/251‑1 (MQ=255)                                                                                                                                                                   ttcggaaaaaaGAGCCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTAt                                                                           <  2:414421/244‑1 (MQ=255)                                                                                                                                                                    gCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATc                                                                          <  1:42865/250‑1 (MQ=255)                                                                                                                                                                                  ccGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                           >  2:522010/1‑251 (MQ=255)                                                                                                                                                                                   cgcccccgcccgtccccgggcGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTTTTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTa                                                          <  1:325659/231‑1 (MQ=255)                                                                                                                                                                                               gcACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAc                                              <  1:76165/250‑1 (MQ=255)                                                                                                                                                                                                     gACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcg                                        <  2:280385/251‑1 (MQ=255)                                                                                                                                                                                                       gggggcacaACAGCGGCGTGATCCATGCCGGGGTCTATTACGCGTCCGGGAGCCTGAAGGCGCAGTTTTGCCGAGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGGTGCCACGTCCGATCTCGAAATGGAACGGATACGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAAGGGCTTAACGCCGACGAACTgcgcgag                                      <  2:547199/246‑1 (MQ=255)                                                                                                                                                                                                       cGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgag                                      <  1:485780/251‑1 (MQ=255)                                                                                                                                                                                                              caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                               <  1:43768/251‑1 (MQ=255)                                                                                                                                                                                                              caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                               <  1:17123/251‑1 (MQ=255)                                                                                                                                                                                                               aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAcc                              <  1:129924/251‑1 (MQ=255)                                                                                                                                                                                                                         tGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAcc                    >  1:52246/1‑251 (MQ=255)                                                                                                                                                                                                                             ccATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGc                <  1:437472/251‑1 (MQ=255)                                                                                                                                                                                                                               aTGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTc              >  1:481129/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcg             <  1:316195/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcggc           >  1:7637/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggTCTATTACACGTCCGGCAGCCTGAAGGAGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGACCGCGAACAGAATATCACAGGGCTCggcggc        >  2:292787/1‑250 (MQ=255)                                                                                                                                                                                                                                          tATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtttt   >  1:227086/1‑251 (MQ=255)                                                                                                                                                                                                                                           attccacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAttttt  <  2:430389/247‑1 (MQ=255)                                                                                                                                                                                                                                              acacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTTGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGc                                                                                            <  2:369254/158‑1 (MQ=255)                                                                                                                                                                                                                                              acacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGc                                                                                            >  1:369254/1‑158 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   AGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGA‑GCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGT‑CGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTT  >  NC_000913/2789913‑2790395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑