Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,992	C→A	intergenic (‑209/‑26)	leuS ← / → ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,992	0	C	A	100.0%	246.4 / NA	62	intergenic (‑209/‑26)	leuS/ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/31);  total (31/31)

ATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCGAACGTGATATCGACGCACTGGTGGAACAGGCGCGCGAGCGCGTAATAAAAACAGGGGAGTTAACGCGAACCGAGGTCGATGAGCTGACGCGAGCTGTCAGACGTGACCTGGAAGAGTTCGCCATGAGCTATGAAGAGAGCCTGAAAGAAG  >  NC_000913/674755‑675234                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   aTCTCTTCCTGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTTCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAActtcc                                                                                                                                                                                                                                         <  2:73730/249‑5 (MQ=255) aTCTCTGCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGAc                                                                                                                                                                                                                                          <  1:404661/248‑1 (MQ=255)     ctTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCggg                                                                                                                                                                                                                                     <  1:405187/249‑1 (MQ=255)     ctTCCGGGCGGTATTGCTCTTGCATGGCAGACAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCACAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCggg                                                                                                                                                                                                                                     >  1:81562/1‑249 (MQ=255)            gCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAAc                                                                                                                                                                                                                            <  2:151683/251‑1 (MQ=255)            gCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAAc                                                                                                                                                                                                                            <  1:538080/251‑1 (MQ=255)            gCGGTATTGCTCTTGCATGGCAGCCAGAGGTCCTGTTTTCAATACGGCTACCAATGTAGCGTTGCGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAACACAGCCTTTCGCTCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTACTTTACAGAGTTTGTGGGCGTATTAGCAAAGCACGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAAc                                                                                                                                                                                                                            >  2:118559/1‑251 (MQ=255)                     ctctTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTAAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccgg                                                                                                                                                                                                                   >  1:370424‑M1/1‑243 (MQ=255)                                    cAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaat                                                                                                                                                                                                    <  1:4333‑M1/251‑24 (MQ=255)                                           ccTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggat                                                                                                                                                                                             >  1:343733‑M1/1‑221 (MQ=255)                                              gTTTTCAATACGGCTACAAATGCCGCGTTGAGGAGGATTTTCAGATCCGCATAGCATAGCCTAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAAAACGTCTATTATTATAGTAAGTTACCGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggccga                                                                                                                                                                                           >  2:315924‑M1/1‑218 (MQ=255)                                                 ttCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtatt                                                                                                                                                                                        <  1:468752‑M1/250‑36 (MQ=255)                                                   cAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattcc                                                                                                                                                                                      <  2:119708‑M1/250‑38 (MQ=255)                                                     aTACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggc                                                                                                                                                                                   >  2:461582‑M1/1‑211 (MQ=255)                                                          gCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgat                                                                                                                                                                              <  2:399419‑M1/251‑46 (MQ=255)                                                             aCAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataag                                                                                                                                                                           <  2:94370‑M1/251‑49 (MQ=255)                                                                aaTGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaag                                                                                                                                                                        <  2:134414‑M1/251‑52 (MQ=255)                                                                     aGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggatt                                                                                                                                                                   <  1:289723‑M1/251‑57 (MQ=255)                                                                           gAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttac                                                                                                                                                             >  1:462572‑M1/1‑189 (MQ=255)                                                                           gAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGGTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgacgaaatatgggatgtattccggcacgataagaagggattattta                                                                                                                                                              <  2:456095‑M1/250‑62 (MQ=255)                                                                                 gtTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgggccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgct                                                                                                                                                       >  2:320584‑M1/1‑183 (MQ=255)                                                                                            ccGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactc                                                                                                                                            >  1:388568‑M1/1‑172 (MQ=255)                                                                                            ccGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagact                                                                                                                                             <  2:6958‑M1/250‑79 (MQ=255)                                                                                             cGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactc                                                                                                                                            >  1:350582‑M1/1‑171 (MQ=255)                                                                                                  agcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaac                                                                                                                                      >  1:180372‑M1/1‑166 (MQ=255)                                                                                                  agcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCACGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggctcgataagaagggattatttacgtcgctgacgcagactcatcaac                                                                                                                                      <  1:454584‑M1/251‑86 (MQ=255)                                                                                                     atagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacaca                                                                                                                                   <  1:68586‑M1/251‑89 (MQ=255)                                                                                                       agcCCACACGTCCGCGTCAAAACAGCCTTTCGCGAACTCGCCGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagc                                                                                                                                 >  1:82812‑M1/1‑161 (MQ=255)                                                                                                                 tCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcatcaaaacc                                                                                                                        >  2:314552‑M1/1‑151 (MQ=255)                                                                                                                  ccGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaa                                                                                                                      <  2:270503‑M1/251‑102 (MQ=255)                                                                                                                      gTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaaca                                                                                                                   >  1:219710‑M1/1‑146 (MQ=255)                                                                                                                          aaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgcc                                                                                                              >  2:383223‑M1/1‑142 (MQ=255)                                                                                                                          aaaCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgcc                                                                                                              <  2:108274‑M1/251‑110 (MQ=255)                                                                                                                                    tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCCAGGAACAAAGAACGTCTATTATTATAGCAAGTTAACGCCCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgccgctgacgcagcctcatccactcatcagcaaaaccaaacaatgccgtcagcacc                                                                                                     <  1:96‑M1/250‑119 (MQ=255)                                                                                                                                    tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcaccc                                                                                                    >  1:62433‑M1/1‑132 (MQ=255)                                                                                                                                      gcgcACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccac                                                                                                  <  1:264867‑M1/251‑122 (MQ=255)                                                                                                                                       cgcACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccac                                                                                                  >  2:331633‑M1/1‑129 (MQ=255)                                                                                                                                            tCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggc                                                                                            >  2:500105‑M1/1‑124 (MQ=255)                                                                                                                                              gACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggcc                                                                                           >  1:71472‑M1/1‑122 (MQ=255)                                                                                                                                                 gTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtg                                                                                                                                                                                                                 >  1:544631‑M1/1‑119 (MQ=255)                                                                                                                                                 gTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtg                                                                                                                                                                                                                 <  2:544631‑M1/129‑11 (MQ=255)                                                                                                                                                    gAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgcc                                                                                    >  2:374995‑M1/1‑116 (MQ=255)                                                                                                                                                       atgatgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggt                                                                                 >  1:80694‑M1/1‑113 (MQ=255)                                                                                                                                                            gCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgt                                                                             <  2:24106‑M1/250‑143 (MQ=255)                                                                                                                                                                 gATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtat                                                                       >  1:181922‑M1/1‑103 (MQ=255)                                                                                                                                                                     actcaTACATTAATTTACAGAGTTTGTGGGCGTAATAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtg                                                                   <  1:554741‑M1/249‑153 (MQ=255)                                                                                                                                                                         aTACATTAATTTACAGCGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcataaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtg                                                                 >  1:281210‑M1/1‑95 (MQ=255)                                                                                                                                                                                aaTTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtg                                                        >  1:35613‑M1/1‑88 (MQ=255)                                                                                                                                                                                    gacAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGGCCCGGGAGATGGAACGgcatccggtgagcgtgccgaaatatgggatgtatgccggcgcgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtc                                                     <  2:307‑M1/249‑167 (MQ=255)                                                                                                                                                                                      cAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtacgggtggtggc                                                  >  2:300656‑M1/1‑82 (MQ=255)                                                                                                                                                                                           tttGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcac                                             >  1:98052‑M1/1‑77 (MQ=255)                                                                                                                                                                                            ttGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcac                                             <  1:287556‑M1/250‑175 (MQ=255)                                                                                                                                                                                             tgtgGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgt                                           >  1:212818‑M1/1‑75 (MQ=255)                                                                                                                                                                                                   cGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtgg                                     >  1:234718‑M1/1‑69 (MQ=255)                                                                                                                                                                                                     tATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagcagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggtt                                   >  2:105800‑M1/1‑67 (MQ=255)                                                                                                                                                                                                         agcaaagcaaGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaaca                               <  2:159394‑M1/251‑189 (MQ=255)                                                                                                                                                                                                                        aaaGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaact                <  1:47299‑M1/251‑204 (MQ=255)                                                                                                                                                                                                                            aaCGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgg            <  1:500105‑M1/251‑208 (MQ=255)                                                                                                                                                                                                                            aaCGTCTATTATTATAGTAAGGTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacccctcgcgggtgaactgcgg            <  2:134286‑M1/251‑208 (MQ=255)                                                                                                                                                                                                                                   attattatAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggatcat      <  1:422390‑M1/250‑214 (MQ=255)                                                                                                                                                                                                                                      attatAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctctcgggtgaactgcgggatcatcgc   <  1:6232‑M1/250‑217 (MQ=255)                                                                                                                                                                                                                                      attatAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggatcatcgcc  >  1:364028‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   ATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCGAACGTGATATCGACGCACTGGTGGAACAGGCGCGCGAGCGCGTAATAAAAACAGGGGAGTTAACGCGAACCGAGGTCGATGAGCTGACGCGAGCTGTCAGACGTGACCTGGAAGAGTTCGCCATGAGCTATGAAGAGAGCCTGAAAGAAG  >  NC_000913/674755‑675234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑