Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,492,0280CT21.4% 98.7 / 10.3 45Q90Q (CAG→CAA) ppiApeptidyl‑prolyl cis‑trans isomerase A
Reads supporting (aligned to +/- strand):  ref base C (2/31);  new base T (9/0);  total (14/31)
Fisher's exact test for biased strand distribution p-value = 1.23e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCG  >  NC_000913/3491787‑3492275
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          
cAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATc                                                                                                                                                                                                                                                   <  1:20965/251‑1 (MQ=255)
 aaCGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCt                                                                                                                                                                                                                                                  <  1:450262/251‑1 (MQ=255)
         gAGTCGTCCCCTGGTAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGt                                                                                                                                                                                                                                           <  2:366747/250‑1 (MQ=255)
         gAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGt                                                                                                                                                                                                                                           <  1:267967/250‑1 (MQ=255)
              ggCACCGGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTGTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTTCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAAcc                                                                                                                                                                                                                                     <  2:282139/251‑1 (MQ=255)
                             ttATCGGCAACGTCCATGCCTTTTACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGGTTTTTT‑TTCTGCATTTGCTCGGTGGAACCGCCCCCCCGAATCAt                                                                                                                                                                                                                      >  1:441482/1‑251 (MQ=255)
                                 cGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAg                                                                                                                                                                                                                  <  1:463075/251‑1 (MQ=255)
                                      aCGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑gg                                                                                                                                                                                                            <  1:281612/251‑1 (MQ=255)
                                        gTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑GTTGTTTTTTTTTTCTGCATCTCCTCGGTGAAACCGCCCCCCTGGAAATTAAAGCCA‑GGa                                                                                                                                                                                                           >  1:95065/1‑250 (MQ=255)
                                            aggcctttagccacTTGACCAAAGACCGCGTAACCGAGATCACGCTGACCATGGGCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGGGGTACGTGCCATCGCGATGGTGCCATGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCGGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGAc                                                                                                                                                                                                      <  2:155106/241‑1 (MQ=255)
                                                 tttCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑GTTGGGTTTTTTTGCTGCCTCTGGCCGGTGGAACCGCCGCCCTCAATccttaaacccggactgacagggg                                                                                                                                                                                                  >  1:460401/1‑229 (MQ=255)
                                                    ccccccgtgaCCAAATACCGCGTAACCGAAAGCACGCTGACCATGGTCTAGGAAGTCGTTATCGGCAACGTTGATAAAGAACTGGCTGTTGGCGCTGTCTTTGTCAGCGGTGCGGGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCACTGTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAAGCATAAAGCCA‑GGAATGACGCGGTGa                                                                                                                                                                                               <  2:293209/241‑1 (MQ=255)
                                                     accacTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑GTTTGTTTTTTTTTCTGCATCTTCTCGGGGAAACCGCCCCCCCGGATCATAAAACCAAGGAA‑GACGCggggga                                                                                                                                                                                              >  1:164271/1‑246 (MQ=255)
                                                            tACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAgt                                                                                                                                                                                      <  1:295375/250‑1 (MQ=255)
                                                                 aaTACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTgtt‑gtt                                                                                                                                                                               <  1:242433/251‑1 (MQ=255)
                                                                   tACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑GTTGGGTTTTTTTGCTGCATCTTCTCGGGGGAAACGCCGCCCTGGATCATAAAACCA‑GGGAAGACGCGGGGA‑AAGGGAGTgtt‑gtttt                                                                                                                                                                             >  1:235220/1‑249 (MQ=255)
                                                                     ccGCGTAACCGAACTCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATaa                                                                                                                                                                           <  1:213143/251‑1 (MQ=255)
                                                                     ccGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATaa                                                                                                                                                                           <  1:111149/251‑1 (MQ=255)
                                                                            aCCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGc                                                                                                                                                                    <  1:377081/251‑1 (MQ=255)
                                                                               gAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGGTTTTTTTGCTGCATCTGCTCGGTGAAAACGCCGCCCTGGATCATAAAGACA‑GGGATGACGCGGGGG‑AAGGGAGTGTT‑GTTATAAAAAACGCTGt                                                                                                                                                                 >  1:169321/1‑251 (MQ=255)
                                                                                     aCGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGGCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACAt                                                                                                                                                           <  2:380471/251‑1 (MQ=255)
                                                                                              aTGGGCAAGGAAGGCGTTATCGGCAACGGTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACaa                                                                                                                                                  <  2:512675/251‑1 (MQ=255)
                                                                                                 gTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTTTTTTGCATCTGCTCGGGGAAAACGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGGGA‑AAGGGAGAGTT‑GTTTTAAAAAACGCTGTTTACATAAtaaacaa                                                                                                                                                  >  1:53748/1‑242 (MQ=255)
                                                                                                   cAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGGTTTTTT‑TTCTTGATCTTCTCGGGGAAACCGCCGCCCCGAATCATAAAAACA‑GGAATGACGCGGGGG‑AAGGGAGAGGT‑GGTTTAAAAAACCCTGGTCACATAAACGAAAAAGttt                                                                                                                                             >  1:502709/1‑251 (MQ=255)
                                                                                                                aTCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACt                                                                                                                                 <  2:169321/250‑1 (MQ=255)
                                                                                                                 tCGGCAACGTTGAAAAAGAACTGGCTGGGGGCGCTGTCTTTGGCAGCGGTACGTGCCATCGCGATGGTGCCAGGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGTTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GGTATAAAAACCGCTGTTCACATAATCGAAAAAGTTTTGCACAGACACTg                                                                                                                                <  2:393302/250‑1 (MQ=255)
                                                                                                                  cggcagtgTTGATAAAGAACGTGCTGGTGGATCTTGCTTTGTAAGCGGTACGGGCCATCGTAATTGTGCCAGGCGTGTTGCGCATGCCGTTATCGGTGTCATTTTTGATTCGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGTCCTTAATCATAAAGCCA‑GGAATGACGAGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGGTTTGCACTGACACTGgc                                                                                                                              <  2:380012/244‑1 (MQ=255)
                                                                                                                     cAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCt                                                                                                                           <  1:301626/251‑1 (MQ=255)
                                                                                                                            gATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCTTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGtt                                                                                                                    <  2:474158/251‑1 (MQ=255)
                                                                                                                                 aGAACTGGCTGGTGGTGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCAGTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATcc                                                                                                               <  2:490845/251‑1 (MQ=255)
                                                                                                                                      tggctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGGTTTTTTTGCTGCATCTGCCCGGTGAAAACGCCGCCCCGAATCATAAAACCA‑GGGATGAAGCGGGGG‑AAGGGAGGGTT‑GTTTTAAAAAACCCTGTTCACATAAACTACAAAATTTTTACCAGACACTGGAGCGTTTTGTTTATCCaaccc                                                                                                          >  1:524464/1‑247 (MQ=255)
                                                                                                                                       ggctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTc                                                                                                          <  1:171948/250‑1 (MQ=255)
                                                                                                                                               gcgcTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGAt                                                                                                 <  1:97641/251‑1 (MQ=255)
                                                                                                                                                            cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTTATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAg                                                                                    <  2:95065/251‑1 (MQ=255)
                                                                                                                                                                                       cACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGGTTTTTTTTCTGCCTCTGCTCGGGGGAAACGCCGCCCTGGATCATAAAACCA‑GGAAGGACGCGGGGG‑AAGGGAGTGTTTGTT‑TAAAAAACCCTGTTCCCATAATTGACAAAGTTTTGTACAGACACTGGCGCCTTTTGTTTTTTCCGCTCCACTTCGATGTTACCAGCTGGGGGTGTCAACAATACATGCGGGTccccc                                                         >  2:328238/1‑250 (MQ=255)
                                                                                                                                                                                                   gcAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGGTTTTTT‑TTTTGCATCTGCTCGGGGAAAACGCCGCCCTGAATCATAACACCA‑GGGATGACGCGGTGGGAAGG‑AGGGTT‑GTTTTAAAAAAAGCTGTTCACATAATCGGCAAAAATTTGCACAGAAACAGGCGCTTTTTTTTTATCTCGCACCCGTTCTATTTTACCACATGAGGTTGTGAAAAATACGTGCGGGTGCCCTCTCTCTGccctt                                             >  2:285800/1‑248 (MQ=255)
                                                                                                                                                                                                    agacatgTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATtg                                            <  1:402982/245‑1 (MQ=255)
                                                                                                                                                                                                                  cTTCATTTTTGATTGGCGGG‑CTTTGTTTTTTTTGCTGCTTCTTCTCGGTGCAACCGCCGCCCTGAATTATAAAGACT‑GGCATGACGCGGGGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCTACAAAGATTTGTACACACACTGGCGCTTTTTGTTTATTCAGCTCACGTTCGATGTTACCAGCTGGGGGTGTCAACAAAACATGCGGGACCCCTTTCGCTGCCATTGCTTCGGGggaa                                 >  2:27526/1‑248 (MQ=255)
                                                                                                                                                                                                                     cATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGagaaagagca                           <  1:327396/251‑1 (MQ=255)
                                                                                                                                                                                                                        ttttGATTGGCGGG‑TTTGCTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGagaaagagcaga                         <  2:223307/250‑1 (MQ=255)
                                                                                                                                                                                                                                ggCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGa                 <  1:313222/250‑1 (MQ=255)
                                                                                                                                                                                                                                  cGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGaaa               >  2:6562/1‑250 (MQ=255)
                                                                                                                                                                                                                                          ggtttTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATACAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGCT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAAcag           >  2:158893/1‑247 (MQ=255)
                                                                                                                                                                                                                                          ggtttTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAAcag           <  1:158893/247‑1 (MQ=255)
                                                                                                                                                                                                                                                ttCTGCTGCATCTGCGCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATcg  <  2:235220/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          
CAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGG‑TTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCA‑GGAATGACGCGGTGA‑AAGGTAGTGTT‑GTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCG  >  NC_000913/3491787‑3492275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑