Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,1570AT21.2% 148.2 / 10.4 67Y82F (TAT→TTT) tqsAautoinducer 2 exporter
Reads supporting (aligned to +/- strand):  ref base A (23/29);  new base T (14/0);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.12e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGT  >  NC_000913/1673917‑1674406
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                  
cAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGCTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTcc                                                                                                                                                                                                                                                         <  1:464885/251‑1 (MQ=255)
 aaaGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATTCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCcg                                                                                                                                                                                                                                                        <  1:320098/251‑1 (MQ=255)
        atcatcACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGTTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCaa                                                                                                                                                                                                                                                 <  1:535997/251‑1 (MQ=255)
                 cTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGGTTTT‑TGGGA‑CCAT‑CATCGTGATGGCGG‑TGGGGTTGCTATTTA‑CTTATCTTGGGTTC‑GCGCTCAAa‑ga‑ggtg                                                                                                                                                                                                                                        >  1:535125/1‑246 (MQ=255)
                    aaTGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGGTTTT‑GATGA‑CCAT‑CATCGGGATGGCGG‑TGGGGTTGCTATTTG‑CTTATCT‑GGGTTCCGCGCCCAAC‑GA‑GTTGAcgcg                                                                                                                                                                                                                                   >  1:107591/1‑251 (MQ=255)
                       ggCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGGCGGGTTT‑GTTTA‑CCCT‑CATCGTGGTGGGGG‑TGGGGTTGCTTTTTG‑CTTTTAT‑GGGtttccagctcaaaaaggtggagcgggc                                                                                                                                                                                                                                  >  1:97841/1‑226 (MQ=255)
                        gCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑TATGA‑CCCT‑CATCGGGATGGCGA‑TGGTGGTTCTATTAG‑CTTATCT‑TGGGTCCGCGCTCAAA‑GA‑GTTGACGCGG‑ACg                                                                                                                                                                                                                              >  1:246033/1‑251 (MQ=255)
                                aTCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑AATCGTGGTGGCGG‑TGGTGTTGCTATTAG‑CTTATTTTGGGTTC‑GCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTAACGcc                                                                                                                                                                                                                     >  1:288676/1‑250 (MQ=255)
                                                          cATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGAcgcc‑gc                                                                                                                                                                                         <  2:515989/251‑1 (MQ=255)
                                                                     gCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTTGATTTT‑TGTGA‑CCAT‑CATCGTGACGGCGG‑TGGGGTTGGTATTAG‑ATTTTCT‑TGGGTCCGCGCCCCAC‑GA‑GTTGACGCGG‑ACG‑GTACCGCAATATCCC‑AACTCTATTATGACGCC‑GCCGCAAACGCtt                                                                                                                                                                              >  2:544898/1‑251 (MQ=255)
                                                                       ggTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGGATTT‑GATGA‑CCAT‑CCTCGTGATGGCGG‑TGGGGTTGCTATTTG‑CTTATCT‑GGGTTTCGCGCTCCAC‑GG‑GGTGAAGCGG‑GCG‑TTACCCCAAAATTGCCAAC‑CTATTTTGTCGCC‑GCCGCAAGCGCTTg                                                                                                                                                                             >  1:333820/1‑250 (MQ=255)
                                                                            ccGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGAGTGCCGCGTGTACTGGCGGAGTTGATTTT‑TTAGC‑CCAT‑CATCCTGGTGGCGG‑TGGTGTTTGTTTTGA‑CTCTTCT‑GCGGTCCGCGCTCCAC‑CG‑GGTGAAGCGG‑ACG‑TTACCACTATATCCC‑AACTCTTTTATAACGCC‑GCTgacaacacttggaccgt                                                                                                                                                                       >  1:452040/1‑235 (MQ=255)
                                                                              gTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑TATGA‑CCAT‑CATCGGGGTGGCGG‑TGGGGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCg‑ttt                                                                                                                                                                    >  1:346972/1‑250 (MQ=255)
                                                                                tttGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGGGTCCATTTT‑TTTGA‑CCCT‑CCTCGGGGGGGGGG‑TGGGGGTTCTATTAGACTTTTTT‑GGGT‑CCGCGCCCAAC‑AA‑GGTGTCGCGG‑ACG‑GTTCCGCCATATCGC‑AACTCTCTTTTGAGACC‑CCTGCAAGCGCCTGAAcacttttt                                                                                                                                                                   >  2:242563/1‑244 (MQ=255)
                                                                                        cgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGGTTTT‑GATGA‑ACAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTTTCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑GTACCGCAATATCGC‑CACTCTATTATGACGCC‑CCTGCAAGCGCCTGAACCG‑TTGTTGCAAcgcg                                                                                                                                                          >  1:479050/1‑251 (MQ=255)
                                                                                              catcGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑TTTGA‑CCAT‑CATCGGTATGGCGG‑TGGGGGTGCTATTAG‑CTTTTAT‑TGGGTACGCGCTCCACAGG‑GTTG‑CGCGG‑GCGGTTAC‑GCAAAATCGC‑CAATCTATTATGACGCC‑GCTGCCAGCGGTTATACCG‑ttgttgtaaaccggagggg                                                                                                                                                    >  2:457847/1‑238 (MQ=255)
                                                                                                  gTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑TTTGA‑CCCT‑CATCGGGGTGGCGGGTGG‑GTTGCTATTAG‑CTTTTCT‑GGGGTCCGCGCCCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATTGC‑CACTCTTTTTTGAAGCC‑GCTGCCAGCGCTTGAACCG‑TTTTTGCAAAGCGGAGGGGTTgg                                                                                                                                                >  2:431314/1‑250 (MQ=255)
                                                                                                                cGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGGTTTT‑GATGA‑CCCT‑CCTCGTGATGGCGA‑TGGTGGTGCTTTTAG‑CTTATTT‑GGGGTTCGCGCTCAAC‑GA‑GTTTACGCGG‑GCG‑TTACCGCAATATCGC‑CACCCTATTATGACGCC‑GCTGCAAGCGCTTGTAACG‑TTGTTGCAAAGCGGAGGGGTTGT‑CGGCTCAGTTTAcc                                                                                                                                 >  1:104428/1‑251 (MQ=255)
                                                                                                                 gCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCa                                                                                                                                <  2:534619/251‑1 (MQ=255)
                                                                                                                   attattTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGc                                                                                                                              <  1:544898/251‑1 (MQ=255)
                                                                                                                      atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑GTTTTCT‑GGGGTTCGCGCTCAAC‑GG‑GTTGACGCGG‑ACG‑GTACCGCAATATTGC‑AACTCTATTATGAACCC‑GCTGCAAGCGCCTGAACCG‑GTGTTGCCAAGCGTAGGGGTTGA‑CGTCTCAGTTGACCCGCTgg                                                                                                                           >  1:447458/1‑251 (MQ=255)
                                                                                                                       tttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGGCGATTTT‑GATGA‑CCAT‑CCTTGTTATGGCGA‑TGGTGTTGGTCTTAA‑TTTTTCT‑GGGTTCCGCGGCCAAC‑AA‑GATGACGCGGGAC‑‑TTACCGCAATTTCGC‑AACTATTTTATTCCGCCTGC‑GCGAGAGCTTGAACCG‑TTTGTGCCACGCGTCGGGactggcctctccgtttgtccgctggg                                                                                                                           >  2:138959/1‑226 (MQ=255)
                                                                                                                        ttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTTTCT‑GGGGTTCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTAACGCAAAAACGC‑AACTCTATTATTACGCC‑GCTTCCAGCGCTTTAACCG‑CTGTTGCAAAGCGGAGGGATTTC‑CGGCTCCGATGACCAGCGGgcg                                                                                                                         >  2:185226/1‑251 (MQ=255)
                                                                                                                              cTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGCTTTT‑TATTA‑CCAT‑CATCGTGATGGCGG‑TGGTGGTGCTCTTAG‑CTTATCT‑GGGTTCCGCGCTCCAA‑GA‑GTTGACGCGG‑ACG‑TTACCGCCATATCGC‑CACTCCATTTTGACCAC‑GCTGCCAGCGCTTTAACCG‑TTGTTGCAACGCGTAGGGATTGG‑CTTCTCACTTgtaccgctcgggcattat                                                                                                                   >  2:79966/1‑234 (MQ=255)
                                                                                                                              cTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGGGTCGATTTTTGA‑GA‑CCAT‑CATCGCGATGGGGG‑TGGTGTTGCTCTTAG‑CTTTTTT‑TGGGTCCGCGCTCAAC‑GG‑GTTGACCCGG‑ACG‑TTACACCAATATCGC‑CAATCTATTTTGACGCCCGC‑GCCAGCGCTTGAACCG‑TTGTTGCTACGCGTCGGGGTTGGGCG‑CTCAGTTGGCCAGCTGGCGCATtat                                                                                                                   >  2:95032/1‑251 (MQ=255)
                                                                                                                              cTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGGGACGAATTT‑TATTA‑CCAT‑CATCGCGGTGGCGA‑TGGCGTTGCTATTAGTCTT‑TCTTGGATTCC‑CGCTCAAA‑GA‑GGTGCGGCGG‑GCG‑TTACCGCAACATCCC‑AAATCCATTATGACGCC‑GCTCCCAACGCTTTAAACGCTTATTGCA‑CGCGTTGGGATTGA‑CGtcttcgttgtacagctgggcgattat                                                                                                                   >  1:220492/1‑228 (MQ=255)
                                                                                                                                    ttCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGGGTCGAATTT‑TAAGA‑CCCC‑CATCGGGAAGGCGA‑TGGTGTTTCTATTAG‑CTTTTTT‑GGGGTTCGCGCTCAAC‑GG‑GTTGACGCGG‑ACG‑CTACCCCACTATCGC‑AAATCTATTTTTACCCC‑CCCGCAAGCGCTTTAACCG‑TTTTTGCAAAGCGTAGGGACTGA‑CGGCCCAGGTGACCCGCTGGCGCCTTAAAttttt                                                                                                             >  1:379470/1‑248 (MQ=255)
                                                                                                                                     tCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGGGGCGGTTTT‑TATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATTT‑GGGGTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑GTTCCCCCATATCGC‑AACTCTATTATGACGCC‑GCTGCCAGCGCCTGAACCG‑TTGGTGCCAAGCGGAGGGATTTA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTTATc                                                                                                            >  1:38803/1‑251 (MQ=255)
                                                                                                                                            ccGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAAcgc                                                                                                     <  1:459045/251‑1 (MQ=255)
                                                                                                                                            ccGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGCGTGGCTTTT‑‑TTCATCCCT‑CATCCTGATGGCGG‑TGGGGCTGCTATTAG‑CTTTTCT‑GGGGTCCCCGCTCCAC‑GA‑GGTGACGCGG‑TCG‑GTACCGCCATATCCC‑AACCCTTTTATGACGAC‑CCTCCCAGCGCTTGTACCC‑TTTTTTCACCGCGTAGGGGTTGA‑CCGCTCACTTGGCCAGccgggctactttattgatccgcacgc                                                                                                     >  2:107874/1‑226 (MQ=255)
                                                                                                                                              gCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGGCGATTTT‑TATGA‑CCAT‑CCTCGTGATGGCGA‑TGGGGTTGCTATTAG‑CTTATCT‑GGGTTCCCCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGGAGCC‑CCCGCAAGCGCTTGAACCG‑TTTTTGCCACGCGGAGGGGTTGA‑CGTCCCATGTGACCAGCTGGCGCATTATTTTTATCCGCACgcgg                                                                                                   >  2:517155/1‑251 (MQ=255)
                                                                                                                                                  gTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTTCCGAGTTT‑GGTGA‑CCATACA‑CGGGAAGGCGG‑TGGTGTTGGCATTAG‑CTTTTTTTGGGTTT‑GCGCTCAAA‑GAATTTAAGGGGG‑GCG‑T‑ACCCCCATATCGC‑AACTCCATTATGTCGCC‑CCTGGCAGCGCTTGAACCG‑CTGGTGCAACGCGTCTGGATTGA‑CGTCCTAGTTGACCAGCTGGCGGATTATATTGATCCGAACgcggcg                                                                                                 >  1:167098/1‑249 (MQ=255)
                                                                                                                                                             ggTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTc                                                                                     <  1:30637/250‑1 (MQ=255)
                                                                                                                                                             ggTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGGTGATTTT‑GGTGG‑CCCT‑CATCGTGGTGGCGG‑TGGGGTTTCTATTAG‑CTTATCT‑GGGGTCCGCGCTCCAA‑GA‑GGTGTCGCGG‑GCG‑TTTCCGCCATATCTC‑AACTCTATTATGACGCC‑GCTGCAAGCCGTTTAAACG‑TTGTTTCCAAGCGGAGGGATTTG‑CGGCTCAGTTGTCCCGCTGGCGCAGTATATTTATCCGCAcggcgcggtggagttgcccc                                                                                    >  2:44083/1‑233 (MQ=255)
                                                                                                                                                              gTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑CA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATTACGCC‑GCTGCAAGCGCTTGAAACG‑TTGTTGCAACGCGTAGGGGTTGA‑CGTCTCCGTTGACCCGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCAc                                                                                   >  1:377813/1‑251 (MQ=255)
                                                                                                                                                              gTCCGCTGGCGTGTGCCGCGTGTACTGGCGGCGGCGATTTT‑GGTGG‑CCCA‑CATCGTGATGGCGG‑CGGGGTTGCGAATAG‑ATTATTT‑GGGGTCCGGGATCAAC‑GG‑GGTGAAGCGG‑ACG‑TTACCGCCATATCGC‑AACTCCTTTATTACGCC‑CCCGCAAGCGCTTGGACCG‑TTGTTGCAACGCGAAGGGAATGA‑CGGCCAAGGTGACCCACTGGCGCATTATATTGATCCggaccccgaggtgtccgttcccac                                                                                   >  2:151861/1‑228 (MQ=255)
                                                                                                                                                                     ggCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATAGTGATCCGAACGCGGCGATGACGTTGCTCACCAACt                                                                              <  1:380756/249‑1 (MQ=255)
                                                                                                                                                                     ggCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GAAGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTTTTAA‑CTTATCT‑TGGGTCCGCGCTCAAC‑GG‑GTTGACGCGG‑GCG‑TTACCGCAATATCGC‑AACTCTATTTTGACGCC‑CCTGCCAGCGCTTGAACCG‑TTGTTTCAACGCGTAGGGGATGA‑CGGCTCAGGTGACCAGCTTGCGCATTATATTGATCCGAACgcggcggtggcggttctccccaaattt                                                                            >  1:376988/1‑230 (MQ=255)
                                                                                                                                                                        gtgtGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCCT‑CATCGTGATGGCGC‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑CCG‑ATCCCGCAATATCGC‑AAATCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCCACGCGTATGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTTATCCGAACACGGCGATGACGTTGCTCACCAACTTAttt                                                                         >  1:460380/1‑250 (MQ=255)
                                                                                                                                                                            gCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGc                                                                     <  1:353894/251‑1 (MQ=255)
                                                                                                                                                                              cgcgTGTACTTGCTGTGTCGATTTT‑GATGT‑CCAT‑CATCGTGATGGCTA‑TGGTGTTGCTATTAG‑CTTAGCT‑GGGTTCTTCGCTCATC‑GA‑GTTGTCGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑TCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCGCAGTTGACCAGCTGGCACATTATATTGATCCTAACTCGGCGATGACATTGCTCACCAACTTATTGTCGCat                                                                   <  2:518152/251‑2 (MQ=255)
                                                                                                                                                                                     aCTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCt                                                             <  1:191675/250‑1 (MQ=255)
                                                                                                                                                                                       tGGCGGGGACGATTTT‑GAAGA‑CTAT‑CATCGTGATGGCGA‑TTGTGTTGCTATTAG‑CCTATAT‑GGGGTCCGCGCTCAAC‑GC‑GTTGCCGCGG‑ACG‑TTACCGCAATATCGC‑CACTATATTATGAAGCC‑GCTGCAAGCGCCTGAACCC‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCCGATGGCTCATGATATTGATCCGAACGCGGCGATGACGTTGCTCACAAACTTATTTATGCAGTTATCTAAt                                                          >  2:481947/1‑251 (MQ=255)
                                                                                                                                                                                        ggCGGTGTCTATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAAt                                                          <  2:531137/250‑1 (MQ=255)
                                                                                                                                                                                        ggCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAAt                                                          <  2:429684/250‑1 (MQ=255)
                                                                                                                                                                                            gtgtCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCAt                                                     <  1:517155/251‑1 (MQ=255)
                                                                                                                                                                                              gtCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGt                                                   <  1:82149/251‑1 (MQ=255)
                                                                                                                                                                                              gtCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCA‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGt                                                   >  2:82149/1‑251 (MQ=255)
                                                                                                                                                                                              gtCGATTTT‑GATGA‑CCAG‑CAGCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGTCGATGACGTTGCTCTCCAACTGATTGACGCAGTTATCTAATTCCATg                                                    <  2:417924/250‑1 (MQ=255)
                                                                                                                                                                                                cGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGtc                                                  <  1:34964/250‑1 (MQ=255)
                                                                                                                                                                                                     tt‑GATGA‑CCAT‑CATCGTGATGGCGA‑TTGTGTTGCTATTAG‑CTTATCT‑GGTTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTTCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCaa                                             <  2:461731/250‑1 (MQ=255)
                                                                                                                                                                                                           ga‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGGCGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATCtttt                                       >  2:932/1‑251 (MQ=255)
                                                                                                                                                                                                            a‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATAttttt                                      <  2:354843/251‑1 (MQ=255)
                                                                                                                                                                                                              ccAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTa                                     <  2:479050/251‑1 (MQ=255)
                                                                                                                                                                                                               cat‑catCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTAt                                    <  1:290284/251‑1 (MQ=255)
                                                                                                                                                                                                                at‑catcGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTAtt                                   >  1:366815/1‑251 (MQ=255)
                                                                                                                                                                                                                   catcGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATtgc                                 >  2:68439/1‑251 (MQ=255)
                                                                                                                                                                                                                   catcGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATtg                                  <  1:240603/250‑1 (MQ=255)
                                                                                                                                                                                                                    atcGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATtgct                                >  1:34976/1‑251 (MQ=255)
                                                                                                                                                                                                                            ggCGA‑TGGTGTTGCGATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACg                         <  2:22483/250‑1 (MQ=255)
                                                                                                                                                                                                                                a‑TGGTGTTGCTTTTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGCCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTgctgctaccgttggt                    >  2:26749/1‑242 (MQ=255)
                                                                                                                                                                                                                                a‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCt                    <  1:398472/251‑1 (MQ=255)
                                                                                                                                                                                                                                a‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTACGCGCTCAAC‑GA‑GTTGACGCCG‑ACG‑TTACAGCAATAGCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCATCTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTTACGGTGCt                    <  2:470789/251‑1 (MQ=255)
                                                                                                                                                                                                                                   ggTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTg                   <  1:231753/250‑1 (MQ=255)
                                                                                                                                                                                                                                    gtgtTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGtt                 >  1:315133/1‑251 (MQ=255)
                                                                                                                                                                                                                                            aTTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTAtgct           <  1:431314/249‑1 (MQ=255)
                                                                                                                                                                                                                                              tAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCTG‑ACG‑TTACCGCAAGATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATgctgct        <  1:479101/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     aTCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAAGATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGt  <  2:172227/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                  
CAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTT‑GATGA‑CCAT‑CATCGTGATGGCGA‑TGGTGTTGCTATTAG‑CTTATCT‑GGGTTCCGCGCTCAAC‑GA‑GTTGACGCGG‑ACG‑TTACCGCAATATCGC‑AACTCTATTATGACGCC‑GCTGCAAGCGCTTGAACCG‑TTGTTGCAACGCGTAGGGATTGA‑CGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGT  >  NC_000913/1673917‑1674406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑