| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,234,233 | 0 | T | A | 23.4% | 106.7 / 10.0 | 48 | Y162F (TAT→TTT) | nhaB | Na(+):H(+) antiporter NhaB |
| Reads supporting (aligned to +/- strand): ref base T (29/7); new base A (0/11); total (29/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGC > NC_000913/1233984‑1234474 | aCCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGCTGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCATCGGTACGGGAAGAGGCTACGCGGTGATAAATACCAt > 2:400563/1‑250 (MQ=255) cGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATaa > 1:465626/1‑250 (MQ=255) aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GAc > 1:111543/1‑251 (MQ=255) aGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAAc < 1:209295/251‑1 (MQ=255) aagaaAGCGCCAAAATTCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑CCCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATCCCATAAAAACC‑GACTGCAACGCt < 2:475771/251‑1 (MQ=255) aaTCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAAc > 2:359305/1‑234 (MQ=255) aaTCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCAGAAAAAc < 1:359305/234‑1 (MQ=255) ccAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATcaccac > 1:524688/1‑251 (MQ=255) aaaaTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGCTCACCACCg > 2:2528/1‑251 (MQ=255) aaaaTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCg > 2:121021/1‑251 (MQ=255) aaaTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGc > 1:216056/1‑251 (MQ=255) aaaTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGc > 1:274519/1‑251 (MQ=255) agccagccGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGAGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCacgacg > 2:194961/1‑251 (MQ=255) ggatgatCGGTTTCTGTGGTTCGCCAACCTTGGTCATACCGCCGCCTAATGCGGTGCCGAC‑CCCGGCATGCATCATCAGGCTAGGCAGAAAG‑CCGCGGAA‑CTGTTCCAGAACCACTTTGTAATGTTTGTGGATATGATTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTCCGCGGTGAAAAATACCATAAAAGCC‑GACTGCAACGCTGATCCCCCCCGCCACGACGGTTAAGGCATCGAgg < 1:52249/250‑1 (MQ=255) atgatCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GAATGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGa > 2:335775/1‑250 (MQ=255) gatCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAAcg > 2:249986/1‑251 (MQ=255) tCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAgg > 1:236940/1‑251 (MQ=255) gTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGc > 1:417170/1‑250 (MQ=255) gTTCCCCCCCCATGGTCATTCCCCCCCCTAAGGCGGTGCCGCC‑CCCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATCCCATAAAAACC‑GACTGCAACGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCa < 1:10667/251‑1 (MQ=255) ccgccccaccgggTCATTCCCCCGCCTAATGGGGTGCCGAC‑ACCGGCATGCATCATCAGGCTCCGCAGAAAG‑CCGCGGAC‑CTTTCCCAGAACCTTGTTGAAATGCTGGTCGATATGACTATCGTGTTGCAGG‑ACGGTGTATTCGGTACGGGAAGAGGCTACGCGGTGATAAATACAAAAAAACCC‑GACGGCAACGCGGATCACAACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAgc < 2:232694/240‑1 (MQ=255) caccaTGGTCTTTGCGCCGCCTAATGCGGGCCCGAC‑CCCGGCTTGCATCATCAGGCTACGAAAAAAG‑CCACGGAA‑CTGTCCCAGAACCCTTTTGTAATGCTTGTCGATATGCTTATGGTTTTCCGGG‑TCGTTTTCTGCGGTACGGGAAGAGGCTACGCGGGGAAAAATCCCAAAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCa < 1:474484/251‑1 (MQ=255) cgccgcCTAAGGCGGTCCCGCC‑ACCGGCATGCATCATCAGGCTCCGC‑GAAAGGCCAGGGAA‑CTTTTCCAGACCCCCTTTGTAATGCTTTTCGATATGACTTCCGTCTTGCAGG‑TCGGTGTTTTGGGTACGGGAAGGGGCTCCGCGGTGAAAAACACCATAAAAACC‑GACTGCACCGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGc < 1:399942/251‑1 (MQ=255) ccaaTGGGGTGGCGACGACCGGCATGCATCACCGGCCTCCGCA‑AAAG‑CCCCCGAATCTGTTCCAGAACCCTTTTTTAATGCTGTTCGTTATGACTGT‑GGCTTGCAGGGTGGGGGCTTTCGTTAGGGGAAGAGGCAACGCGGTGA‑AAAACCCAAAAAAACCCGACGGCACCGCGGATCCCCACCGCCACGACGGTTAAGGCAGCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGAcag < 2:155883/249‑1 (MQ=255) ttGCGGCGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTAAGCGGTGATAAATACCATACAAACC‑GACAGCAACGCTGACCACCACCGCCACGACGGTTAAGGCATAGAGGAACGCGGAGAGGAAGGAAGCCGCCACGCAAAAAGAGAGCGAcagc > 2:50423/2‑248 (MQ=255) gCGGGGCCGAC‑CCCGGCATGCACCACCAGGCTACGAAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGCCGATATGCTTATCGTCTTGCGGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGAAAAATACCAAAAAACCC‑GACTGCAACGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCAt < 1:217598/251‑1 (MQ=255) ccGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTgg > 1:344036/1‑250 (MQ=255) ggCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGCGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCCTTTTGGAGCGAATGc > 2:504265/1‑251 (MQ=255) gcatgcatCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCt > 1:305247/1‑251 (MQ=255) gtcaccatctGGCTACGCAGAAAG‑CCGCGGAA‑CGGTCCCAGACCCCTTTTGTATGGTTTGTCGATATGACTAGCGTGTGGCAGT‑TCGTTGTCTTGGGAACGGAAAGAGGCTACGCGGTTATAAAACCCAAAAAACCC‑GACGGCAACGCTGACCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTaa < 2:527506/246‑1 (MQ=255) catcaacgggCTACGAAAAAAG‑CCACGAAA‑CTGTCCCAAAACCATTTTGTAATGTTTGTCAATATGTCTATCGTCTGGCGGG‑TCGGTGTCTGCGGTCGGGGAAGAGGCGCCGGGGTGAAAAATACCAAAAAAACC‑GACTGCAACGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACa < 2:305247/243‑1 (MQ=255) atcaGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATc > 2:200991/1‑173 (MQ=255) accaGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCCCTTTGTATTGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCCCCACCGCCACGACGGTTAAGGCATc < 1:200991/171‑1 (MQ=255) ccaGGCTACGCAGAAAC‑CCACGGAA‑CTGTTCCAGACCCACTTTGAAATGTTTGTCGATAGGACTATCGTCTTGCAGG‑TGGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATCCCAAAAAACCC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCaaa < 1:121021/250‑1 (MQ=255) ccttctactactacctccACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATAACATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAAc > 1:1992/17‑251 (MQ=255) aCGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCCCTTTGTAATGCTTGTCGATATGACTACCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCGCCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTa < 1:335775/250‑1 (MQ=255) cGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGaacagca > 2:119404/1‑250 (MQ=255) ggAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGaacagcaac > 1:156509/1‑251 (MQ=255) gaactttccaaaacccattttGTAAGGCTTGTCGATATGACTAGCGTCGTGCGGG‑TGGGTTTCTTGGGTAGGGGAAGAGCCTACGCGGTGAAAAATACCAAAAAAACC‑GACGGCACCGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGaacagcaaca < 2:236940/233‑1 (MQ=255) tttCCAGAACCTCTTTGTAATGTTTGTCGATATGCCTATCGGCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTGCGCGGTGATAAATCCCAAAAAAACC‑GACTGCACCGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTg < 1:300345/249‑1 (MQ=255) gTTCCAGAACCATTTTGTAATGGTTGTCGATATGACTATCGTCTTGCGGG‑TGGGTGTCTTCGGTCCGGGAAGGGGCTACGCGGTGATAAATACCAAAAAACCC‑GACTGCAACGCGGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGt < 2:465626/251‑1 (MQ=255) ccAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTc > 2:161502/1‑251 (MQ=255) aaCCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACGGCAAACGGGAAAATATGAACAGCAACAGCTGTTTCATa > 1:252876/1‑250 (MQ=255) cTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAAt > 2:409866/1‑249 (MQ=255) aTGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCg > 2:99406/1‑251 (MQ=255) tGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGccacc > 1:455115/1‑251 (MQ=255) gatgcctaccttttgcgaGG‑TGGGTGTCTTGGGTAGGGGAAGGGGCTCCGCGGTAAAAAATCCAAAAAAAACC‑CACGGCACCGCTGATCCCCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAAc < 1:466106/234‑1 (MQ=255) ggTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTgccc > 2:277382/1‑250 (MQ=255) aTAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAACGAGAGCGACAGCAGCATTTTGGAGCGACTGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGCGTTCcg > 2:289791/1‑251 (MQ=255) tAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCcgc > 1:157742/1‑251 (MQ=255) | ACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGAC‑ACCGGCATGCATCATCAGGCTACGCAGAAAG‑CCACGGAA‑CTGTTCCAGAACCACTTTGTAATGCTTGTCGATATGACTATCGTCTTGCAGG‑TCGGTGTCTTCGGTACGGGAAGAGGCTACGCGGTGATAAATACCATAAAAACC‑GACTGCAACGCTGATCACCACCGCCACGACGGTTAAGGCATCGAGGAACGCGGAGAGGAACGCAGCCGCCACGCAAAAAGAGAGCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGC > NC_000913/1233984‑1234474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |