| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 505,440 | 0 | C | A | 20.0% | 143.5 / 12.6 | 62 | A176E (GCA→GAA) | ushA | 5'‑nucleotidase/UDP‑sugar hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (40/8); new base A (0/12); total (41/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.00e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATG > NC_000913/505199‑505690 | gATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTgg > 1:302638/1‑251 (MQ=255) ccGAAACTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAAc > 1:69900/1‑251 (MQ=255) cGAACCTGCTTTTCGCGGCATGAATCTGGTGGGCTATGACGCGATGGCGATAGGTAATCATGCATTTGATAATCCGCTCACCGTATTACGCCCGCAGGAAAAGTGGGCCAAGATCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AACCCGTGGGCGCTGGTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGCAACAGCAAAAATTGG‑Taccc > 2:487723/1‑248 (MQ=255) gAACCTGCCTTTCGCGGTATGAATCTGGAGGGCTATGACGCGACCGCCATCCGTCATAATGACTTTGATCATCCGCCCACCGTATTACGCCAGCAGGAAAACCGGGCAAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑ACACCGTGGGCGCTGTTTAAC‑CGTCACGATCTGAAACTTG‑CCGGTATTGGGCTGCCAACCG‑ATGACCCCGCACAACTTGG‑TAcccc > 2:179440/1‑251 (MQ=255) gAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAAccc < 1:453832/251‑1 (MQ=255) aCCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑gg > 2:312321/1‑251 (MQ=255) ccTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TATCCC‑GGa < 1:340387/251‑1 (MQ=255) gATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATa < 1:321405/251‑1 (MQ=255) gcgGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACt < 1:168539/251‑1 (MQ=255) gAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGAGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCACCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GAAATA‑CTTCACTGCTATCg > 2:505778/1‑251 (MQ=255) aaTCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCg > 1:463420/1‑250 (MQ=255) cTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAAtt > 2:307410/1‑251 (MQ=255) aTGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCCCCGTATTACGACAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATCTCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGCCACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCCCTGATATCGAATTTCGTAAGccc < 2:193302/251‑1 (MQ=255) aTGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGccc < 2:69900/251‑1 (MQ=255) ccgcgATGGCGATCGGTAATCATGAATTTTATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCccgcc < 2:523011/250‑1 (MQ=255) cGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATgaagcg > 2:500675/1‑251 (MQ=255) ggTAATCATGAATTTGATAATCCGCTCACCGTATTCCGCCGGAAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAGGTACTGGCGAGCGCCTGTTA‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTAAAAATTG‑CCGTTATTGGGCTGACACCCG‑ATGACACAGAAAAAATTGG‑TACCCC‑GAAATA‑CTTCACTGAGATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCt < 1:98564/251‑1 (MQ=255) aTTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAgg > 2:537135/1‑251 (MQ=255) tGATAATCCCCTCACCGTATTCCCCCAGCAGAAAATGGGGGCCAAGTCCCCTTTGCTTTCCGCGAATATCTCCAAAAAAAGTACTGGCGACCGCTTTTTA‑AAACCGTGGCCGCTGTTTAAG‑CGTCAGGATCTAAAAATTG‑CCTTTATGGGGCTGACACCCG‑ATGACCCAAAAAAAATTGG‑AACCCC‑GAAATA‑CTTCCCTGATATCGATTTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAg < 1:332837/250‑1 (MQ=255) tCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGCAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑ATTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGAGATTAAGGAGCTGCAacagac > 1:37869/1‑250 (MQ=255) ggtattccccaagcagAAAAAGGGGGCAAGGTCCCCGTTGCTTCCCGCGAATATCTACAAAAAAAGTACGGGGGAGCCCTTGTTA‑AAGCGGGGGGCGTTGTTAAAG‑CGACAGGATAAGAAAAGTG‑CGGTGATGGGGCGGA‑AACCGGAGGAAAAAAAAAAAATGGG‑AAACAA‑GAAAGA‑CTCCAGGGATATGAAATTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGaaa < 2:127713/240‑1 (MQ=255) ttacccacggaagaaagggTGGGCAAAGTTCGCTTTGTTTTCGGCGAATATCTGCAAAAAAAGGAGTGGCGGTCGCCTTTTA‑AAACGGTGGTCGTTGTTTAAC‑CGACAGGATATGAAAATTG‑CCGTTTTGGGGCTAAACCCCG‑ATGACAC‑CAAAAAATTGGGAAACCC‑GAATTC‑CTTCTCGGATATCGAATTCCGAAAACCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAg < 2:379650/232‑1 (MQ=255) attccGCCAGCAGAAAAAGTGGGCCAAGTCCCCGTTGCTTTCCGCGAATATCTACAAAAAAAGTACTGGCGAGCGCCTGTTA‑AACCCGTGGGCGTTTTTAAAG‑CGTAAGGATCTGAAAATTG‑CCGTTATTGGGCGGACACCCG‑ATGACACAGAAAAAATGGG‑TACCCC‑GGAATC‑CTTCTCTAATATCGATTTTGGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGc < 1:63054/247‑1 (MQ=255) aTTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCCGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAACAATTGG‑TACCCC‑GGCATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGTTGTTTCATGAGCTGCAACAGACAGTAAAGc > 2:109502/1‑251 (MQ=255) gCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGAc > 1:309352/1‑251 (MQ=255) ccAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGCAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGAc > 1:486077/1‑250 (MQ=255) aaaaaggggcccaggtccccGTTGTTTCCCGGGAATATCTACAAAAAAAGTACTGGCGAGCCCCTGTTT‑AACCCGGGGGCGTTTTTAAAG‑CGTCAGGATCTAAAAATTG‑CCGTTATGGGGCTGACAACCG‑ATGACACAGAAAAAATTGG‑TACCCC‑GGAATC‑CTTCACTGATATGGATTTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATtattat < 1:29709/234‑1 (MQ=255) aaaaGGGGGCCAAGTTCCCGTTGCTTCCCGCGAATATCTACCAAAAAAGTACGGGGGGGCGCTTTTTT‑AACCCGGGGGCG‑TTTTTAAGGCGTCAGGATCTAAAATTTG‑CCGTTATGGGGCTAACACCCG‑AGGACACAGAAAAAATTGG‑TACCCC‑GGAATC‑CTCCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATc < 1:19158/251‑1 (MQ=255) gTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCgcgg > 2:228496/1‑251 (MQ=255) ggggCCAAGTCCCCGTTGTTTCCCGCGAATATCCACCAAAAAAGTACGGGCGGGCCCTTGTTA‑AACCCGGGGGCGCTGTTTAGG‑CGGAAGGATCTAAAAATTG‑CCGTTATGGGGCTGACACCCG‑ATGACACAGCAAAAATTGG‑TACCCC‑GAAATA‑CTCCACTGATATCGAATTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCgcggc < 1:114535/250‑1 (MQ=255) gggCCAATTTCCCGTTGCTTCCCGCAAATATCAACCAAAAAAGTCCTGGCGGGCGCTTGTTT‑AAACCGGGGGCGTTGTTTAGG‑CGTCAGTATCTAAAAATGG‑CCGTTTTGGGGCTGAAAACCA‑ATGACACAGAAAAAATTGG‑TA‑CCC‑GGAATTTCTCCACTAATAGCGATTTTCTAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCgcggcg < 1:178860/251‑1 (MQ=255) ccAAGTGCCCATTGCTTTCAGCCAATATCTACCCGCAACGTACTGGCGAGAGCCTGTTT‑AAACCGTGGCCGCTGCTTAAG‑CGTCAGGACCTGAAAATTG‑CCGTTATTGGGCTGCCAACCG‑ATGAAACAGCCAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGCATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATAGCGGCGAcc > 2:443817/1‑251 (MQ=255) caattgccctttGCTTCCCGAGAATATCTACCAAAAAAGTCCTGGGGAGCGCTTGTTT‑AACCCGGGGGCGCTGTTTAAG‑CGTCAGTATCAAAAAATTC‑CCGTTATTGGGCTGACAACCG‑ATGACACAAAAAAAATTGG‑TACCCC‑GGAATC‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGAccc < 1:300187/241‑1 (MQ=255) aaGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGAccc > 1:66209/1‑250 (MQ=255) ccGTTGCTTCCCGCAAATTTTTCCCAAAAAAGTACTGGGGGGCGC‑TGTTTTAAACGGGGGGCTCTTTTTAAG‑CGTCAGGATCT‑AAAATTGGCCGTTATTGGGATGACAACCG‑ATGACCCACAAAAAATTGG‑TACCCC‑GAAATA‑CTTCCCTGAAAACGAATTTGGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATggg < 1:352387/251‑1 (MQ=255) cTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGTCAtt > 1:453123/1‑250 (MQ=255) atatCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCAATATGGGGCATTACGATAATggg > 2:292303/1‑250 (MQ=255) tCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTg > 1:31801/1‑249 (MQ=255) aaaGAACTGGCGAGCGACTATTT‑AAAACGTGGGCGCTGATTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAACATTGG‑GAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGAAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCTTTACGATAATGGTGAGCACGGctct > 2:409281/1‑251 (MQ=255) tACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACg > 1:272311/1‑251 (MQ=255) aCTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGc > 1:47047/1‑251 (MQ=255) gCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCg > 1:289660/1‑251 (MQ=255) gCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCg > 1:31718/1‑251 (MQ=255) gcCTATTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAt > 2:58152/1‑251 (MQ=255) cccttttt‑AAACCGTGGGCGCTGTTAAAG‑CGTGAGTATCAAAAATTTC‑CCGTTATTGGCCTGACACCCG‑ATGACACAG‑AAAAATTTG‑CAACCCCGAAATA‑CTCCACTGATATCAATTTTCGTAGGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAt < 1:312321/246‑1 (MQ=255) aCCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGCTGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCCGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTTAGCACTGCTCTAACGCACCGGACGCTGTGgagaggg > 2:306892/1‑248 (MQ=255) cTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGCTTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGct > 1:424094/1‑251 (MQ=255) tttAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcc > 2:83316/1‑251 (MQ=255) cGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCgg > 2:198514/1‑251 (MQ=255) aGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCAAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGTTGGCGCGCGCGCTGCCTGCCGGATCg > 1:273434/1‑251 (MQ=255) aaaaTTG‑CTTTTATGGGGCTGACACCCG‑ATGACACAGAAAAAATTG‑‑TAACCCCGAAATT‑CTTAACGGATATCGTATTTGGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgat < 1:307410/251‑1 (MQ=255) atggacGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTAACTGATATCGAATTTCGTAAGCCCGCCGATGAAGAGAAGCTGGCGATTCAGGAGCTGCAACAGACAGAAACGCCAGACATTATTATCGCGGCGACACATATGGGGCATTACGATAATGGTGAGCACTGCTCTAACGCACCGGGCGATGTGGAGATGGAACGCGCTCTTCCTGCCGGAGTGCTGGCgatgat > 2:255252/6‑251 (MQ=255) g‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgt > 1:297622/1‑251 (MQ=255) ccGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgtc > 1:305792/1‑251 (MQ=255) gTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCgg > 1:467623/1‑251 (MQ=255) aTTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCggtgg > 2:161791/1‑251 (MQ=255) gggCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTc > 1:422846/1‑250 (MQ=255) gaaacccG‑ATGACACAGAAAAAATTGA‑TACCCC‑GAATTA‑CTTCTCTGATATAGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTc < 1:79348/244‑1 (MQ=255) gACAACCG‑ATGACACAGGAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCAAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGCGGTCCCTCGc > 1:100505/1‑251 (MQ=255) gACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGc > 1:34826/1‑251 (MQ=255) aacaccgaatgcaccacAAAAATTGGGTACCCC‑GAAATC‑CTTCATGGATATCTAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTc < 1:114130/233‑1 (MQ=255) aCCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGCATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCa > 1:435287/1‑248 (MQ=255) acacAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTc > 1:439326/1‑251 (MQ=255) cAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGCTATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATg > 2:204071/1‑251 (MQ=255) cAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCAGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATg > 2:126724/1‑251 (MQ=255) | GATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGACGCGATGGCGATCGGTAATCATGAATTTGATAATCCGCTCACCGTATTACGCCAGCAGGAAAAGTGGGCCAAGTTCCCGTTGCTTTCCGCGAATATCTACCAGAAAAGTACTGGCGAGCGCCTGTTT‑AAACCGTGGGCGCTGTTTAAG‑CGTCAGGATCTGAAAATTG‑CCGTTATTGGGCTGACAACCG‑ATGACACAGCAAAAATTGG‑TAACCC‑GGAATA‑CTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATG > NC_000913/505199‑505690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |