Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	41 [1]	[0] 40	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	1 (0.010)	40 (0.590)	39/492	0.4	98.8%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

AGGGCCATTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGC  >  NC_000913/3079166‑3079640                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 cgataaacagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAggg                                                                                                                                                                                                                                   >  1:340003‑M2/235‑250 (MQ=255)          gacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGgtgt                                                                                                                                                                                                                         <  2:300720‑M2/26‑1 (MQ=255)           acgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATGGAGCAGGGAAACCGGTGtt                                                                                                                                                                                                                        <  1:325042‑M2/27‑1 (MQ=255)                gtcagaaattacactcaccagtaattcattatctgtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGa                                                                                                                                                                                                                    <  2:499598‑M2/31‑1 (MQ=255)                       attacacccaccagtaattcatcatcgggaatttctggcagttcaaatacgcgcaaacgcacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggctaattgattaaatcaagtgtcgagttttggatcgccaatatagttattaatccacatcagtggtttatccgtaaccAAGAAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTg                                                                                                                                                                                                            >  2:202886‑M2/213‑251 (MQ=255)                          acactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGaa                                                                                                                                                                                                          <  1:4022‑M2/41‑1 (MQ=255)                                  cagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAtt                                                                                                                                                                                                 <  1:140177‑M2/50‑1 (MQ=255)                                         tcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTtat                                                                                                                                                                                           >  1:314718‑M2/195‑250 (MQ=255)                                                    aatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAg                                                                                                                                                                                <  1:217835‑M2/67‑1 (MQ=255)                                                            gcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcctttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGat                                                                                                                                                                        >  2:160812‑M2/176‑250 (MQ=255)                                                                   aaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttaaccgtcacaAAGCAATTGAGCAGGGAGACCGGTCTGTTGAAAACAGTGAATTATTAGTGTCTGATATAACATTGAGTTATAGATATaacaac                                                                                                                                                                <  2:188995‑M2/83‑1 (MQ=255)                                                                              gacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATa                                                                                                                                                     <  1:23229‑M2/94‑1 (MQ=255)                                                                                             tgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGAt                                                                                                                                        <  2:56758‑M2/107‑1 (MQ=255)                                                                                              gccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGc                                                                                                                                      <  1:455984‑M2/109‑1 (MQ=255)                                                                                                  taaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGGGAATTATTAATTTCTTATATAACATTGATTTATAGATTTAAAAACAAATGTCAATAAGCATATCGTGATGCATAtt                                                                                                                                 >  2:289954‑M2/138‑251 (MQ=255)                                                                                                    aatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttgggtcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAAGCATATCGTGATGCATATTa                                                                                                                                >  2:193137‑M2/136‑250 (MQ=255)                                                                                                             aactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGat                                                                                                                      >  1:63815‑M2/127‑251 (MQ=255)                                                                                                              actttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGat                                                                                                                      >  2:17498‑M2/126‑250 (MQ=255)                                                                                                                       ttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgcgttttggatcgccaaaatagttattaatcaatatcagtggtttatccgtcacaAAGCAAATGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTATTATATCACCTTTAGTTATAGATATAACAACAAATGTCAATAAGCATAACGTGATGCATATTAAATAATTGTTATTTATTgcc                                                                                                            >  2:551384‑M2/117‑250 (MQ=255)                                                                                                                          atttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAAGTGATATTTATTGCatat                                                                                                         <  1:377856‑M2/138‑1 (MQ=255)                                                                                                                             tttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaa                                                                                                       >  2:208220‑M2/111‑250 (MQ=255)                                                                                                                                 cctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATGACATAATTGATATTTATTGCATATAAATAtt                                                                                                  <  1:428356‑M2/145‑1 (MQ=255)                                                                                                                                      gtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                                                                              >  1:475718‑M2/102‑250 (MQ=255)                                                                                                                                           cagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAt                                                                                                                                                                                                  >  1:540154‑M2/97‑145 (MQ=255)                                                                                                                                           cagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAt                                                                                                                                                                                                  <  2:540154‑M2/49‑1 (MQ=255)                                                                                                                                               gtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaaca                                                                                    <  1:526809‑M2/159‑1 (MQ=255)                                                                                                                                                        cggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATc                                                                           >  1:294621‑M2/84‑251 (MQ=255)                                                                                                                                                         ggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCt                                                                          <  1:160812‑M2/169‑1 (MQ=255)                                                                                                                                                         ggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCt                                                                          >  1:546710‑M2/83‑251 (MQ=255)                                                                                                                                                                aattgcttaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt                                                                   >  2:5952‑M2/76‑251 (MQ=255)                                                                                                                                                                   tgcttaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTc                                                                >  2:26471‑M2/73‑251 (MQ=255)                                                                                                                                                                         aataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCaaa                                                          <  1:343905‑M2/185‑1 (MQ=255)                                                                                                                                                                                tgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACat                                                   >  2:156337‑M2/60‑251 (MQ=255)                                                                                                                                                                                 gtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACat                                                   >  2:403275‑M2/59‑250 (MQ=255)                                                                                                                                                                                                  caatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAg                                 <  1:267597‑M2/210‑1 (MQ=255)                                                                                                                                                                                                          ttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCg                          >  1:460814‑M2/34‑250 (MQ=255)                                                                                                                                                                                                            attaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGaa                        <  2:216070‑M2/219‑1 (MQ=255)                                                                                                                                                                                                              taatcaatataagtggtttatccgtcacaAAGCAATTGAGCAGGGCAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCg                     >  1:66311‑M2/30‑251 (MQ=255)                                                                                                                                                                                                                      atcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt             >  1:501731‑M2/22‑251 (MQ=255)                                                                                                                                                                                                                                 atccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGc  >  1:336782‑M2/11‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 AGGGCCATTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGC  >  NC_000913/3079166‑3079640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑