Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	241.5 / NA	65	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (31/34);  total (31/34)

CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560207‑3560687                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         cttcttTTACCATCAACGTGTTAGCAACGTTGAGCTTGTTGGTGACAATGCGCAAATTGCTGTGCTTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCAGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GGACTGGAAGGCAGCGCCGCCCCGCCATGATGGcgc                                                                                                                                                                                                                                         >  2:472066/1‑249 (MQ=255)    cttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                      >  2:46318/1‑246 (MQ=255)        tcccATCAGCGTTTTAGCAACGTTGATATTGTTGGTGACAAGTCGCAAATTGCTGTGTTGGAGCGGTGCGGGCGCTCCCGCTTCGGGCGTGGTGCCGTAACCAATAACCGGGGTCGGGCCTTGGGGGTTTTTCCCCGCCTCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGGGCCACGGGGTGTTAA‑CC‑GACCTGGAAGGCAGCGCCCCACCGCCATGATGTCGCAGGATc                                                                                                                                                                                                                                   <  2:424686/248‑1 (MQ=255)         aCCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcaggctc                                                                                                                                                                                                                                   >  2:481344/1‑241 (MQ=255)                aCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                          >  2:89132/1‑251 (MQ=255)                     ttAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGTGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGc                                                                                                                                                                                                                     >  1:267762/1‑251 (MQ=255)                        gCAACGTTGAGCTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGCTTTGCCCCGcc                                                                                                                                                                                                                    >  2:125155/1‑249 (MQ=255)                          aaCGTTGAGATTGTTGGTGACAATGCACAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                                                                                                                                >  1:377445/1‑251 (MQ=255)                             gTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                             >  1:130874/1‑251 (MQ=255)                                   aTTGTAGGTGAAAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGAGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGAGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGCAGGCGGCGCCGCACCGCCATCATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt                                                                                                                                                                                                       >  2:179275/1‑251 (MQ=255)                                     tgttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                     >  1:111421/1‑251 (MQ=255)                                      gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGGGCCGATATCGATAAACAGCGTCGAGCCTTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                    <  1:4175/251‑1 (MQ=255)                                      gttgGTGACAAGGCGCAAATTGTTGTGTTTGGGCAGTGCGTGCGCTCCCGCTTCGGGCGTGGTGCCGATTTCGATAAACAGCGTGGTGCCATTGGGGATTGGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GACGTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                     <  1:298449/250‑1 (MQ=255)                                          gTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGCACTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGAAgcggcg                                                                                                                                                                                                >  2:333310/1‑251 (MQ=255)                                            gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                               >  1:104386/1‑250 (MQ=255)                                            gACAAGGCGAAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACGGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                                                                                                              <  1:302093/251‑1 (MQ=255)                                                   cgcAATTTGCTGTGATTGAGCGGTGCGGGCCCTCCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                                                       <  1:255967/251‑1 (MQ=255)                                                   cgcAAATTGCTGTGATTGCGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTTCCGATATCGATAAACAGCGTCGAGCCATTGGGGCTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGCGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGTCTg                                                                                                                                                                                       >  2:126731/1‑251 (MQ=255)                                                            gtgggtttGAGCAGTGGGTGCGCTCCCGTTTCGGGGGTGGTGCCGATATGGATAAACAGCGTCGGGCCATTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                              <  1:190873/245‑1 (MQ=255)                                                                       cAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                                   >  2:190006/1‑251 (MQ=255)                                                                               gcgcCACCGCTTCCGGCGGGGTTCCGATATCGTTAACCAGCGTCGGTCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTTTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                            <  1:507265/250‑1 (MQ=255)                                                                                   tACCGCTTCCGGCGTGGAGCCGATATCGATCAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAAAGTATGCGGGCTGACGGCAAAATGCTCTACCAGCTCtt                                                                                                                                                       >  2:418920/1‑251 (MQ=255)                                                                                       gCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                   >  2:172525/1‑251 (MQ=255)                                                                                          tCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                    >  2:394846/1‑183 (MQ=255)                                                                                          ccgggcgggtggcagttatggaaaaaCAGCGTCGGGCCATGGGGGTTTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTGGGTTGGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                 <  1:241461/227‑1 (MQ=255)                                                                                                         atatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCCCCCCTTTGCGGGCGATGCCCTCTTTTTCTTCGGTCTGGGTGGCCTG‑GCGATCGTGCCCCGGCGTGTTAA‑CC‑GAACTGGAAGCCGGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg                                                                                                                                 <  1:450424/251‑1 (MQ=255)                                                                                                           atggATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                <  1:50107/247‑1 (MQ=255)                                                                                                                aaaaaggcgccggcctaTTGGGAATTGGCCCCGCCACTTTGCGGGCGTTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGACCGTGCCACGGCGGGTAAA‑CC‑GAACTGGAAGACAGCGACGCACCGCCATGATGGCGCAGGATCAGGTTTTGCACCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                          <  1:472066/235‑1 (MQ=255)                                                                                                                   cagggtgcggtccgttgggtttttGCCCCGCCCCTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCGGGGGGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                       <  2:55856/230‑1 (MQ=255)                                                                                                                           gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAT‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                               >  1:452444/1‑251 (MQ=255)                                                                                                                           gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATTCACTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACAAGTTCgctcat                                                                                                               >  2:299303/1‑246 (MQ=255)                                                                                                                           gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                               >  2:482693/1‑251 (MQ=255)                                                                                                                              ccgtgggtgttttgcgcggCCACTGTTCGGGCGGTGCGCTCTTTTTCGTCGGTCTGGGTGGCCTG‑GCGAGCGTGCC‑CGGGGGGGTAAGCC‑GAAGTGGAAGCCGGCCCCGAACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGTCATAACCCTGCTGTTTAACCAGTTCGATAATAcc                                                                                                            <  2:137744/233‑1 (MQ=255)                                                                                                                                   gggattttGCTCCGCTACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGAAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATTCCGTtg                                                                                                        <  1:231983/245‑1 (MQ=255)                                                                                                                                      gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAACAGTCCGCTCATACCGttcgtg                                                                                                     >  2:422712/1‑246 (MQ=255)                                                                                                                                         ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATATTAACGTTGTGACGtt                                                                                                 >  2:190011/1‑251 (MQ=255)                                                                                                                                          gtctcccccctttggcggggggtGCGCTCTTTTGCGTCGGTCGGGGTGGCCTT‑GCGATCGTGCCACGGGGTGTTAC‑CC‑GAACTGGAGGGCAGGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                 <  2:267762/228‑1 (MQ=255)                                                                                                                                           gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                               <  1:108712/251‑1 (MQ=255)                                                                                                                                             cccGCCCCTTTGCGGGCGAGGCGCTCTTTTCCTCCGGTCTGGG‑GGCCTTTGCTATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTgtgt                                                                                             <  1:481344/250‑1 (MQ=255)                                                                                                                                               cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttg                                                                                           >  1:184040/1‑250 (MQ=255)                                                                                                                                                   aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                      >  2:460996/1‑188 (MQ=255)                                                                                                                                                   aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                      <  1:460996/188‑1 (MQ=255)                                                                                                                                                     tttGGGGGCGACTCGCTCTTTTTCTTCGGTCTGGGTGCCCTT‑GCGTTCGTGCCACGGCGTTTTAA‑CC‑GAACTGAAAGGCCGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATTACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgtttgttt                                                                                       <  2:537601/249‑1 (MQ=255)                                                                                                                                                        gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                  >  1:518853/1‑251 (MQ=255)                                                                                                                                                          gtgCGATGCGCTTTTTTTCTGAGGTCTGGGTGGCGTT‑GCGATCGTGCCAAGGGGTGTTAA‑CC‑GACCAGGAAGGCAGCGACGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                                                <  1:290146/249‑1 (MQ=255)                                                                                                                                                              gATGCGCTTTTTTTCTTCGGTGTGGGTGGCCTT‑GCGATGGGGCCACGGGGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                            <  2:130874/251‑1 (MQ=255)                                                                                                                                                                  cgctctttttgtggggccgggggggCCTT‑GCGAGGGTGCCACGGCGTGT‑AA‑CCAGACCGGGAGGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                        <  2:93904/228‑1 (MQ=255)                                                                                                                                                                        ttttCTTCGGTCTGGGTGGCCTT‑GCGATCGGGCCCCGGCGTGTAAA‑CC‑GAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                                     <  2:518853/248‑1 (MQ=255)                                                                                                                                                                              taggTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGAAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                               <  1:105720/246‑1 (MQ=255)                                                                                                                                                                               cGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcg                                                           >  2:349601/1‑251 (MQ=255)                                                                                                                                                                                  tCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                                  <  1:57997/177‑1 (MQ=255)                                                                                                                                                                                  tCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                                  >  2:57997/1‑177 (MQ=255)                                                                                                                                                                                    ggggTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                      <  1:295333/250‑1 (MQ=255)                                                                                                                                                                                     gggTGGCCTT‑GCGATTGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGTGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                  >  1:129353/1‑222 (MQ=255)                                                                                                                                                                                     gggTGGCCGT‑GGGATCTTGCCACGTCTGGTTAA‑CC‑AACCTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTTTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATGAttttcgttttc                                                      <  2:484886/250‑1 (MQ=255)                                                                                                                                                                                      ggTGGCCTT‑GCGAGCGTGCCACGGCGTGTAAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgc                                                    <  2:169347/251‑1 (MQ=255)                                                                                                                                                                                        gggCCTT‑GCGATCGGGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                  <  2:170222/250‑1 (MQ=255)                                                                                                                                                                                         ggCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttctaaa                                                  >  1:136201/1‑246 (MQ=255)                                                                                                                                                                                                 cGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                          <  1:89132/251‑1 (MQ=255)                                                                                                                                                                                                  gATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGCATTATTTTCGTTTTCGCGCATTGAGCg                                          >  2:236173/1‑250 (MQ=255)                                                                                                                                                                                                   aTCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                         >  1:260814/1‑250 (MQ=255)                                                                                                                                                                                                            cGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGTAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATATATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCACCaaa                                >  2:259809/1‑250 (MQ=255)                                                                                                                                                                                                            cGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                >  1:391666/1‑250 (MQ=255)                                                                                                                                                                                                               ggtgtTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                            <  1:332549/250‑1 (MQ=255)                                                                                                                                                                                                                  tttAA‑CC‑GAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt                          <  1:227120/249‑1 (MQ=255)                                                                                                                                                                                                                      a‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa                     <  1:157343/251‑1 (MQ=255)                                                                                                                                                                                                                              cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcca                >  2:218470/1‑249 (MQ=255)                                                                                                                                                                                                                                           ccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc  <  1:218470/251‑1 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         CTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTT‑GCGATCGTGCCACGGCGTGTTAA‑CC‑GAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560207‑3560687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑