Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	202.6 / NA	56	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652087‑1652574                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aTGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTTATGTGCACCTCagag                                                                                                                                                                                                                                               <  2:162310/251‑1 (MQ=255) aTGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGAAGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAAAGCATATCATTGATGTTCACCTCag                                                                                                                                                                                                                                                 >  1:257401/1‑249 (MQ=255)              gTTTATACTGGAGTTCGCCCTTGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcag                                                                                                                                                                                                                                     <  2:409105/248‑1 (MQ=255)                  ataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacagaa                                                                                                                                                                                                                              <  2:85259/251‑1 (MQ=255)                    gcTGGAGTTCGCCCTGGAGAACTGTGTGAACTTGGATGGGAGGACATAGATCTGACAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATAGTGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCTCAGGCAAAGTACATTAACGAGAATGAA‑CAAAAAGCCTAATAATGATGGTCACCTCCGAGAGTATGGcagaaacaaaa                                                                                                                                                                                                                             >  2:573714/2‑244 (MQ=255)                    aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTAATAAGCCAGCAATCGACGTCCTCCGGAGCCAGATGACATTAACGCGAATGAG‑CAAAGAGCATATCATTGATGGTACCCTCAGAGAGTATTGCagaaaagaaa                                                                                                                                                                                                                             >  2:28847/1‑250 (MQ=255)                     cTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGACAAAACAGGGCCTTCATCTTATTTAAACAGCAATCGAAGCTATCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGAGGTTCACCTCAAAGAGTATGGccgaaaaaaaaa                                                                                                                                                                                                                            >  2:148172/1‑243 (MQ=255)                              gCCCTGGTGAGCTGAGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGAGGGAACCAAACGGGTGAATTATCTTATTAAGACCGCCATCGACGCTCTCCCGAGTGAGAAGACATTAAAGAAACTGAGGCAAGCAACA‑CTCATTTATGTTCACCTCCGCGAGGAGGGCAGAAAAGAAAAAAAAAAAttc                                                                                                                                                                                                                  >  2:382186/1‑249 (MQ=255)                                   ggTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtt                                                                                                                                                                                                             <  2:205354/251‑1 (MQ=255)                                      gAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTg                                                                                                                                                                                                           <  1:622446/250‑1 (MQ=255)                                        gCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttt                                                                                                                                                                                                        <  1:236671/251‑1 (MQ=255)                                           gtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCTCCTTTGtttt                                                                                                                                                                                                       <  1:608526/249‑1 (MQ=255)                                               gcaattGGATGGGAGGACATAGATCTGAAAAATGGCACAATGATGATCAGGAGCAATTTAGCAAACGACCGTTTCAAGGAACCAAAAACACAGGAGGGAAACACACGGGTCCTTCATCTTTTTCAGCCAACAATCGACGCCCTACGGAGTCAGCTGACATTAACGAGACTGAG‑CAAAGAGCATAACATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAACCCAAAAATGCATCTTTGTTTTTCaccc                                                                                                                                                                                                 >  2:528052/5‑248 (MQ=255)                                                 aCTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTg                                                                                                                                                                                               <  1:429499/251‑1 (MQ=255)                                                     ggATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAg                                                                                                                                                                                            <  1:219378/250‑1 (MQ=255)                                                         gggAGGACATAGATCTGAAAAATGGAACAATGATGATCAGTAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACAGTAACGAGACTGAG‑CAAAGAGCATAGCATTGATGTTCTCCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCGCCTTGGTTTTTCACCCGTAAGTGTCa                                                                                                                                                                                       <  2:192025/251‑1 (MQ=255)                                                           gaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAAACTGAG‑CAAAGAGCATAACATTGATGTTCACCTCAGAGAGTATGGCAGAACCGAAAAAAAAAAAAGCACCTTTGTTTTTCAACCTGAAGAGTCAGc                                                                                                                                                                                     >  1:439993/1‑251 (MQ=255)                                                            aggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCg                                                                                                                                                                                    <  1:622224/251‑1 (MQ=255)                                                            aggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCg                                                                                                                                                                                    <  2:434633/251‑1 (MQ=255)                                                            aggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCATCTTATTAAGCCAACAATCGACGCCCTCCCGAGTCCGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTTACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAAACTGaaatggcag                                                                                                                                                                                      >  2:554320/1‑242 (MQ=255)                                                                       cTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCCTCTTATTAAACCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAAAGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCCGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTTCACCTGAAGTGTCAGCGAGAGGaaaaa                                                                                                                                                                          >  1:126229/1‑250 (MQ=255)                                                                             aaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATgg                                                                                                                                                                   <  1:148172/251‑1 (MQ=255)                                                                                 gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc                                                                                                                                                               <  1:28847/251‑1 (MQ=255)                                                                                      atgatgATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAACGGGGCATTCATCGTAGTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATAACATTAACGGGAATTAG‑AAAAGAGCATATAATTTATGGTCACCTCAGCGAGGGTGGCAGAAAAGAAAAACAAAAATGCACCTTTTTTTTTTAACCTGAAGTGACAGCGGGAGTAAAAAATTATGGGGACCAttt                                                                                                                                                          >  1:316104/1‑251 (MQ=255)                                                                                       tgatgaTCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAAAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAAAATTATGGTGACCCtttt                                                                                                                                                         >  1:607019/1‑251 (MQ=255)                                                                                        gatgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATACGGTCATTCATATTATTAAGCCCGCAAACAACGCTCTCCGGAGTCAGATGGCATTAACGAGACAGAG‑CAAAGAGCATATCATTGATGTTCAACTCAGAAAGGATGGCAGAAAAAAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAATTGTAAGCGGGGGGAAAAAATTATGGTGACCAtttt                                                                                                                                                         >  1:377748/1‑250 (MQ=255)                                                                                            atCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATAGGGACTTTTATCTTATTAAGCCAGCAATCGAACCTCTCCGGAGTCAGACGACATTAAAGAGACTGCG‑CAAAGAGCCTATCATTGATGTTTACCTCCGAGAGTATGGCAGAACAGAAAAACAAAACAGCACCTTTGTTTTTCAACCTGAAGGGTTAGCGGGAGTAAAAAATTATGGGTACCAtttttccct                                                                                                                                                    >  2:237614/1‑246 (MQ=255)                                                                                                    aaaTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCCTTTATCTTATTAACACAGAAATCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAG‑CCAAGAGCCTAAAATTTAAGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCTACCTGTAGTGTTCGCGAGCGAAAAAAAATATGGGGACCATTTTATCGTTGAccca                                                                                                                                             >  2:67192/1‑247 (MQ=255)                                                                                                            cAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAg                                                                                                                                    <  1:554320/251‑1 (MQ=255)                                                                                                                     gTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCCTCTTATTAAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGGAAGGCAGAACAGAAAAACAAAAATGCCCCTTTGTTTTTTAAACTGAAGTGTCAGAGAGAGAAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGGAGAAGTgggg                                                                                                                            >  1:389362/1‑249 (MQ=255)                                                                                                                                              gggAACCAATCGGGACATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGAATGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGAGGGACGCAGCGATAAAACGTGCCGGACTcc                                                                                                  >  1:335200/1‑251 (MQ=255)                                                                                                                                                              attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCata                                                                                  <  1:54028/251‑1 (MQ=255)                                                                                                                                                                       attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCg                                                                           >  1:98087/1‑249 (MQ=255)                                                                                                                                                                            gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTCCCGtt                                                                                                                                                   <  1:349983/172‑1 (MQ=255)                                                                                                                                                                            gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGtt                                                                                                                                                   >  2:349983/1‑172 (MQ=255)                                                                                                                                                                            gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACAt                                                                     >  2:285518/1‑250 (MQ=255)                                                                                                                                                                                 cAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTAt                                                               >  2:350614/1‑251 (MQ=255)                                                                                                                                                                                 cAATCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGATTCCGCCATCGAAAATCATATCAGTCGAGACATACTTAt                                                               <  2:352707/251‑1 (MQ=255)                                                                                                                                                                                  aaTCGACGCTCTACGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCGTATCATTGTTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTCCCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCCTATCAGTCGAGACATACTTATg                                                              >  2:456511/1‑251 (MQ=255)                                                                                                                                                                                   aTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGCAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGc                                                             >  2:51302/1‑251 (MQ=255)                                                                                                                                                                                     cGACGCTCTCCGGAGTCAGATGACATTAACTAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGcc                                                            <  2:389362/250‑1 (MQ=255)                                                                                                                                                                                     cGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCct                                                           <  1:51302/251‑1 (MQ=255)                                                                                                                                                                                              ccGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTc                                                    <  2:532156/249‑1 (MQ=255)                                                                                                                                                                                              ccGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTc                                                    <  1:285518/249‑1 (MQ=255)                                                                                                                                                                                                ggAGTCAGATGCCATTAACGAGCCTGCG‑CAAAGTGCATATCATTGATGTTAACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTACAAAATTATGGTGACCATTTTACCGTTGACTAAATAAGGCAGATGGGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATAAGGCGAGACATACTTATGACTGCTGGACGCtt                                                >  2:178449/1‑250 (MQ=255)                                                                                                                                                                                                ggAGTCAGATGACAATAACGAGACTGAG‑CAAAAAGCATAACATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGGCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTg                                                <  2:607019/251‑1 (MQ=255)                                                                                                                                                                                                      aGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTGTGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCt                                          >  1:627662/1‑251 (MQ=255)                                                                                                                                                                                                          gACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAGCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGt                                       <  2:572667/250‑1 (MQ=255)                                                                                                                                                                                                           aCATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAACATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGc                                     >  2:467009/1‑251 (MQ=255)                                                                                                                                                                                                               tAACGAGAATGAG‑CGAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAACCACAAATGCACCTTTGTTTTTCAACCTGAAGTGTAAGGGAGAGGAAACAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGAAGTGAGACGCAGCGATAAAACGTGCCGGCCTCCGCCATCGAAAATCATATCAGTCAAGACATACTTATACATCCTGGTCGCTGACAGCTGGTGATAAc                                 >  2:240188/1‑251 (MQ=255)                                                                                                                                                                                                                aaCGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAAc                                 <  1:382186/250‑1 (MQ=255)                                                                                                                                                                                                                                  agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAc               <  1:421023/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAt   <  2:345904/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAt   <  2:57716/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAt   >  2:7811/1‑251 (MQ=255)                                                                                                                                                                                                                                               tGTTCACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGGTAACCCGGCAGTTATAGCAAACACGCTGGGCCATg  >  2:275240/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAG‑CAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652087‑1652574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑