Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,549	T→C	intergenic (+63/‑37)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,549	0	T	C	100.0%	209.9 / NA	59	intergenic (+63/‑37)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/29);  total (30/29)

AAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGT  >  NC_000913/536304‑536791                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aaaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAt                                                                                                                                                                                                                                                <  1:385101/250‑1 (MQ=255)  aaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                                                                              <  1:343570/251‑1 (MQ=255)      tGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAgg                                                                                                                                                                                                                                           >  2:335478/1‑250 (MQ=255)               gCAAATTGCCGATAATCCGCATCGCTGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATGCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGa                                                                                                                                                                                                                                   <  1:494016/249‑1 (MQ=255)                cAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAt                                                                                                                                                                                                                                <  1:235586/251‑1 (MQ=255)                  aaTTGCCGATAATCCGCAGCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAttt                                                                                                                                                                                                                              <  1:292829/251‑1 (MQ=255)                  aaTTGACGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAttt                                                                                                                                                                                                                              >  1:395002/1‑251 (MQ=255)                         gATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAagag                                                                                                                                                                                                                       <  1:431743/251‑1 (MQ=255)                             aTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTa                                                                                                                                                                                                                   <  2:629138/251‑1 (MQ=255)                               ccGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                                                                                 >  1:564796/1‑251 (MQ=255)                                      gcggcgAACCGGGAACCGGAGAAATTAAGTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATAAAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAGTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGaaa                                                                                                                                                                                                          <  2:209374/251‑1 (MQ=255)                                           gaaccgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTggg                                                                                                                                                                                                     <  2:366565/251‑1 (MQ=255)                                             accgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGAt                                                                                                                                                                                                   >  1:280774/1‑251 (MQ=255)                                                gggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGAttt                                                                                                                                                                                                 >  1:593576/1‑250 (MQ=255)                                                     cccgagaAAATAATTATTATTATCTCTTTAAGGTAAACGAAAAATCTGGCTACAAAGGTTGGGGTGGG‑GTGGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAGTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGc                                                                                                                                                                                           <  2:363273/248‑1 (MQ=255)                                                                aattaTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTa                                                                                                                                                                                 <  1:426382/250‑1 (MQ=255)                                                                aaTTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAg                                                                                                                                                                                <  1:35333‑M1/251‑2 (MQ=255)                                                                    aTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagt                                                                                                                                                                            >  2:580727‑M1/1‑246 (MQ=255)                                                                          aTCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtagg                                                                                                                                                                      >  1:269018‑M1/1‑240 (MQ=255)                                                                                    ggTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacat                                                                                                                                                            >  1:144047‑M1/1‑230 (MQ=255)                                                                                    ggTAATCGAAAATACTGACTACAACGGTTGTGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCTAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacat                                                                                                                                                            <  1:139913‑M1/251‑22 (MQ=255)                                                                                     gTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatt                                                                                                                                                           <  1:474572‑M1/251‑23 (MQ=255)                                                                                          cGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTa                                                                                                                                                                                                                             >  2:632065/1‑180 (MQ=255)                                                                                          cGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTa                                                                                                                                                                                                                             <  1:632065/180‑1 (MQ=255)                                                                                           gAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcc                                                                                                                                                     >  1:546315‑M1/1‑223 (MQ=255)                                                                                              aaTTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACACCAAACCACCACGGAAGCCGGGTTAGGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGCTCGTGTTGTCCGATTTAAGGAAAACAGAAATTTAAAAGAGGTTCATTATGAACCTGGAATTTATTGGATTAGGCATTCgtagtcgtagtcattagccatttgtgactg                                                                                                                                                   >  2:8229‑M1/1‑220 (MQ=255)                                                                                                 tCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGAATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGCTTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgc                                                                                                                                               >  1:24435‑M1/1‑217 (MQ=255)                                                                                                    gACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgac                                                                                                                                            >  2:399378‑M1/1‑214 (MQ=255)                                                                                                          aaCGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCCGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGACCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttg                                                                                                                                       >  1:499532‑M1/1‑208 (MQ=255)                                                                                                          aaCGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAACGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgg                                                                                                                                      >  2:170271‑M1/1‑208 (MQ=255)                                                                                                          aaCGGTGGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGCTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAACGAAATTTAAACGAGGTTAATTATGAAACTGGGATTTGTTGGCTTAGGCATTAgtagtcgtaggaattagacatttgtgcctgatgcgacgcttg                                                                                                                                       >  2:15970‑M1/1‑208 (MQ=255)                                                                                                                tgggttgggtGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtc                                                                                                                                >  1:447026‑M1/1‑202 (MQ=255)                                                                                                                 gggttgggtGTG‑AATATAATCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattatacatttgtgcctgatgcgacgcttgacgcgtc                                                                                                                                <  1:296980‑M1/250‑50 (MQ=255)                                                                                                                 gggttgggtGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtct                                                                                                                               >  1:161286‑M1/1‑201 (MQ=255)                                                                                                                            g‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcct                                                                                                                     <  2:555602‑M1/250‑61 (MQ=255)                                                                                                                                          aaaCCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTActagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgc                                                                                                       >  1:92754‑M1/1‑177 (MQ=255)                                                                                                                                               accacGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccg                                                                                                  <  1:354407‑M1/251‑80 (MQ=255)                                                                                                                                                     gAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaatt                                                                                            >  1:416625‑M1/1‑166 (MQ=255)                                                                                                                                                              ttACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTGATTGGCTTAGGCATTAgtagtcggaggcattagacagttgtgccggatgcgacgcttgacgcgtcttagcaggcctacatccggtgccggagccggcaattggtgcaca                                                                                    <  2:206705‑M1/250‑94 (MQ=255)                                                                                                                                                               tACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaa                                                                                   <  2:280774‑M1/250‑95 (MQ=255)                                                                                                                                                                           atCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgat                                                                      >  2:274320‑M1/1‑144 (MQ=255)                                                                                                                                                                                            gtgacgcTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGCGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCATAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgcagcttgacgcgtcttatcaggcctacaaccggtgccgcatccgccaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                     >  1:192762‑M1/4‑127 (MQ=255)                                                                                                                                                                                              gacGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGCTCGTGTTGTCCGAATTCAGGCAAAGCGAAATTTAAACGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                     >  2:306508‑M1/2‑125 (MQ=255)                                                                                                                                                                                              gacGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                     >  2:2145‑M1/2‑125 (MQ=255)                                                                                                                                                                                                 gCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGTTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcct                                                                              >  1:253049‑M1/1‑122 (MQ=255)                                                                                                                                                                                                 gCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGTTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcct                                                                              <  2:253049‑M1/221‑100 (MQ=255)                                                                                                                                                                                                           cAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggt                                      <  1:582292‑M1/251‑140 (MQ=255)                                                                                                                                                                                                               gCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGCGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                   >  2:235450‑M1/1‑108 (MQ=255)                                                                                                                                                                                                               gCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                   <  1:235450‑M1/250‑143 (MQ=255)                                                                                                                                                                                                                      tAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggt                           >  1:121396‑M1/1‑101 (MQ=255)                                                                                                                                                                                                                       aGGCCGCTAAGTTGGCAGGGGATCGTGTTTTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTTGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggt                           <  1:502954‑M1/250‑151 (MQ=255)                                                                                                                                                                                                                           cGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggta                                     <  1:75009‑M1/236‑141 (MQ=255)                                                                                                                                                                                                                           cGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggta                                     >  2:75009‑M1/1‑96 (MQ=255)                                                                                                                                                                                                                               aaGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggat                  <  2:54539‑M1/251‑160 (MQ=255)                                                                                                                                                                                                                                   tGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgactgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcttcttatcaggcctacaatgggtaccggatcggtagcccggataagg              >  1:306215‑M1/1‑88 (MQ=255)                                                                                                                                                                                                                                       aGGGGATCGTGTTGTCCAAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTAGTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtt          <  2:395002‑M1/251‑168 (MQ=255)                                                                                                                                                                                                                                         gggATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTActagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgttta        <  2:92754‑M1/251‑170 (MQ=255)                                                                                                                                                                                                                                              cGTGTTGGCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgcctcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctactatgggtaccggatcggtaggccggataaggcgtttacgccg   <  2:396344‑M1/251‑175 (MQ=255)                                                                                                                                                                                                                                               gtgtTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAAGTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgtgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatgttatcaggcctacaatgggtaccggataggtaggccggttaaggcgtttacgccgc  >  1:270869‑M1/1‑76 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   AAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTG‑AATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGT  >  NC_000913/536304‑536791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑