Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,637,436	G→A	intergenic (‑26/‑206)	yhiN ← / → pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,637,436	0	G	A	100.0%	138.2 / NA	37	intergenic (‑26/‑206)	yhiN/pitA	putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

CGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACC  >  NC_000913/3637188‑3637677                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  catgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgtcgtgctgcattttgccgatgacgttatcgcccagcgcgtcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgattctattattgggtcctttggtgaacgatcaacaaagggccactagcacaccttattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGt                                                                                                                                                                                                                                                <  1:229152‑M2/28‑1 (MQ=255) catgtgcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGt                                                                                                                                                                                                                                                <  1:369099‑M2/28‑1 (MQ=255) catgtgcacataaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgagcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgattaacaaagggccactatcacacctgattcctctctaaataccctatccgaGTTTCGCTCCTCTTA‑AAAAAACGGACGt                                                                                                                                                                                                                                                <  1:313595‑M2/28‑1 (MQ=255)      gcacatcaaccacgacgactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAAt                                                                                                                                                                                                                                            <  1:273349‑M2/32‑1 (MQ=255)                      gactgtctggaaatcgccgtgttgaaaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgctgtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGt                                                                                                                                                                                                                           >  1:13926‑M2/203‑251 (MQ=255)                                               aaggtgacatgggtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTTCTa                                                                                                                                                                                                  <  2:64415‑M2/74‑1 (MQ=255)                                                           gtgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTAtt                                                                                                                                                                                      <  2:349742‑M2/86‑1 (MQ=255)                                                            tgacgtgcagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTAttt                                                                                                                                                                                     >  1:148877‑M2/165‑251 (MQ=255)                                                                   cagcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGaa                                                                                                                                                                              <  1:230465‑M2/94‑1 (MQ=255)                                                                    agcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAAt                                                                                                                                                                             <  1:7420‑M2/95‑1 (MQ=255)                                                                     gcattttgccgatgacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAAtt                                                                                                                                                                            <  2:365380‑M2/96‑1 (MQ=255)                                                                                  gacgttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAatt                                                                                                                                                               >  1:222284‑M2/143‑251 (MQ=255)                                                                                     gttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAattatt                                                                                                                                                            <  1:124200‑M2/112‑1 (MQ=255)                                                                                     gttatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAattatt                                                                                                                                                            >  1:225668‑M2/140‑251 (MQ=255)                                                                                       tatcgcccagcgcggcgtgcggcacgggcatttgcagtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAACGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTAtttt                                                                                                                                                          >  2:53701‑M2/138‑251 (MQ=255)                                                                                                                gggcatttgcagtgcttgccgaaggaagagtgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCa                                                                                                                                 <  2:238022‑M2/139‑1 (MQ=255)                                                                                                                          agtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCaaaaa                                                                                                                        >  2:100394‑M2/103‑250 (MQ=255)                                                                                                                          agtgcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactaccacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAATTCaaaaa                                                                                                                        >  1:84825‑M2/103‑250 (MQ=255)                                                                                                                             gcttgccgaaggaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGGAAAGATTCCTCATTGGTCGCACCAGCATCAATAGTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAACAGt                                                                                                                    >  2:134591‑M2/100‑251 (MQ=255)                                                                                                                                tgccgaaggaagattgagtctattattgggtcccttggtgaacgagcaacaaagggccactagcacacctgattcctctctaaattccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATGGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCt                                                                                                                  <  1:194127‑M2/154‑1 (MQ=255)                                                                                                                                        gaagattgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCAc                                                                                                         <  2:147942‑M2/163‑1 (MQ=255)                                                                                                                                        gaagattgagtctattatcgggtcctttggtgaacgctcaacaaagggccactagcacacctgattcctctctaaataccctatccgcGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCAc                                                                                                         >  2:94197‑M2/89‑251 (MQ=255)                                                                                                                                            attgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTg                                                                                                     <  1:235027‑M2/167‑1 (MQ=255)                                                                                                                                              tgagtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGc                                                                                                    <  2:10157‑M2/168‑1 (MQ=255)                                                                                                                                                agtctattattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccg                                                                                                 >  2:138480‑M2/81‑251 (MQ=255)                                                                                                                                                     attattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgccg                                                                                            <  2:14594‑M2/176‑1 (MQ=255)                                                                                                                                                     attattgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgc                                                                                              <  1:369289‑M2/174‑1 (MQ=255)                                                                                                                                                     attattgggtcatttggtgaacgatcaacaaagggccactagcacacctgatacctctctaactcccctctccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGCATTGCAAAGATTCCTCAGTGCTCGCCCCAGCATCAATATTACTAAAAGGAAGAATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgccg                                                                                            >  1:111006‑M2/76‑251 (MQ=255)                                                                                                                                                        attgggtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCccgcgccgcg                                                                                          <  2:110531‑M2/178‑1 (MQ=255)                                                                                                                                                             gtcctttggtgaacgatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTc                                                                                    >  1:233516‑M2/68‑251 (MQ=255)                                                                                                                                                                           gatcaacaaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGcgcc                                                                      >  2:85929‑M2/54‑251 (MQ=255)                                                                                                                                                                                 caaagggccactagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTc                                                                <  1:324364‑M2/204‑1 (MQ=255)                                                                                                                                                                                          actagcacacctgattcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGTAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTc                                                        >  2:333913‑M2/39‑250 (MQ=255)                                                                                                                                                                                                        ttcctctctaaataccctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACT‑AAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTc                                        >  1:68481‑M2/25‑251 (MQ=255)                                                                                                                                                                                                              tctaaataacctatccgaGTTGCGCTCCTCTTA‑AAAAAACGGACGTGACTTGTAACGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATACGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATg                                   >  2:112764‑M2/19‑251 (MQ=255)                                                                                                                                                                                                                                            cttaaaaaaaCGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAAGGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTg        <  2:84825/245‑1 (MQ=255)                                                                                                                                                                                                                                                aaaaaaCGGACGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATAcc  <  1:198592/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  CGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATACCCGCCGCACCAGCGCCTATAATAATGGCATCAAACCTTTCCACGTTGCGCTCCTCTTAGAAAAAACGGGCGTGAATTGTAAAGATTCCTCAGTGGTCGCACCAGCATCAATATTACTAAAAGGAAGTATTTGCCTGAATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTACATTTGTTTGCTGGCCTGGATTTGCATACC  >  NC_000913/3637188‑3637677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑