Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	101.8 / NA	28	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652094‑1652570                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATg                                                                                                                                                                                                                                       <  1:38427/250‑1 (MQ=255)           ataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacagaa                                                                                                                                                                                                                            <  2:375164/251‑1 (MQ=255)           ataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCAAATCATTGATGTTCACCTCAGAGAGGATGGcagaacaga                                                                                                                                                                                                                             >  1:354801/1‑250 (MQ=255)                       gCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGTCATTCATCTTATTAAGCCCGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCAAATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGc                                                                                                                                                                                                                >  1:75209/1‑251 (MQ=255)                        cccTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCa                                                                                                                                                                                                               <  2:372322/251‑1 (MQ=255)                               gAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTg                                                                                                                                                                                                        <  2:354801/250‑1 (MQ=255)                                                   ggaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCCTATCATTGATGTTCACCTCAGAGGGTAAGGCAGAACAAAAAAAAAAAAATGCA‑CCTTTGTTTTTTAACCCGaaaggtc                                                                                                                                                                                     >  2:50203/1‑244 (MQ=255)                                                              aTCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCCTTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAAACTGAAGGGTCAGCGAGAGTaaa                                                                                                                                                                         >  1:49817/1‑250 (MQ=255)                                                                cTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGTCATTCATCTTATTAAAACAGCAATAGACGCTCTCCGGGGGCCAATGAAATTAACGAGAAAGCGCAAAGGGCATATCATTGAGTTTCAACCCCGGGAGTATGGCAGAACAGAAAAAAAAAAATGCA‑CCTTTTTTTTTCAACCCTAAGAGTCAGCgagaacaaaaa                                                                                                                                                                       >  2:155775/1‑243 (MQ=255)                                                                    aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt                                                                                                                                                                  <  2:20381/251‑1 (MQ=255)                                                                            aCAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCa                                                                                                                                                         <  1:186422/251‑1 (MQ=255)                                                                               atgatgATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGTACCAATCGGGTCATTCCTCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTTCACCTCAGAGAGGATGGCAGAACAAAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAAAAATAT‑GGATACCAttt                                                                                                                                                      >  2:197921/1‑251 (MQ=255)                                                                                       cAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTg                                                                                                                                              <  1:64508/251‑1 (MQ=255)                                                                                           agaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGACCAAACGGGTCATTTATATTATTAAGCCAGCCATCGGAACTCTCCGGAGTCAGATGACATTAACGGGACTGAGCAAAAAGCATATCATTGCTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACCAAAATACAACTTTTGTTTTT‑AAACAGCAGTGTCAGAGAGAGTAAAAAATTTTAGGTCAAC‑TTTTACCGTTGACTc                                                                                                                                          >  1:140999/1‑251 (MQ=255)                                                                                                         aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCAATCCTCTTATTAAGCCAGCAATCGACGCTCTCCGGGGTCAGATGACATTAACGAGGCTGGGCAAAGAGCCTATCATTTATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAAACTGAAGTGTAAGCGAGAGGAAAAAAATAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGAtgg                                                                                                                            >  1:107735/1‑250 (MQ=255)                                                                                                                  cACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAGCAAAGAGCATATCCTTTATGTTCACCCCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTTCCGTTGCCTCAATAAGGCAGATGTGGGACGc                                                                                                                     >  2:155668/1‑249 (MQ=255)                                                                                                                                                           atCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc                                                                            >  1:144111/1‑249 (MQ=255)                                                                                                                                                               tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCg                                                                       <  1:264977/250‑1 (MQ=255)                                                                                                                                                                  taAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAGATGCA‑CATTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATCTCAGTCGAGAc                                                                   >  2:138200/1‑251 (MQ=255)                                                                                                                                                                   aaGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGAc                                                                   <  1:197921/250‑1 (MQ=255)                                                                                                                                                                        aGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACtt                                                             >  2:56324/1‑251 (MQ=255)                                                                                                                                                                                aCGCTCTCCGGAGTCAGATTACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgc                                                     <  2:209129/251‑1 (MQ=255)                                                                                                                                                                                         ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCAGTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAG‑GGTGCCCATTTTACCGTGGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCGTCGAAAATCATATCAGTCGAGACATACTTATGCCTGTTGGTCGCTg                                            <  2:101386/251‑1 (MQ=255)                                                                                                                                                                                                   gACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGAAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCAGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGCCAGCTGGTg                                  >  2:277992/1‑251 (MQ=255)                                                                                                                                                                                                                  gAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAtt                     <  1:198515/249‑1 (MQ=255)                                                                                                                                                                                                                     cAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat                  >  1:38269/1‑249 (MQ=255)                                                                                                                                                                                                                      aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc               >  2:278472/1‑251 (MQ=255)                                                                                                                                                                                                                                   ttGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGc  >  2:175418/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCA‑CCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAT‑GGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652094‑1652570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑