Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,640,045	+C	coding (13/534 nt)	rrrQ ←	putative lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,640,045	1	.	C	97.4%	142.9 / ‑2.7	39	coding (13/534 nt)	rrrQ	putative lysozyme
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (15/23);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑T‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTTCTTTGTCC  >  NC_000913/1639795‑1640288                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    cATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑t                                                                                                                                                                                                                                                      <  2:145005/251‑1 (MQ=255)      cAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑t‑ctttt                                                                                                                                                                                                                                                <  1:358815/251‑2 (MQ=255)              tATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTc                                                                                                                                                                                                                                           <  1:245888/249‑1 (MQ=255)                             cGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtct                                                                                                                                                                                                                          >  1:368560‑M1/1‑235 (MQ=255)                                      ggTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatat                                                                                                                                                                                                                  <  1:32230‑M1/250‑25 (MQ=255)                                                     tCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAGCCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaacttt                                                                                                                                                                                                    <  1:309517‑M1/249‑39 (MQ=255)                                                           gTTTCGTATTGGGAAAAACGGTTTTTCCATCCACCACCGTGGCACCCCGACAGATGGTCCATATGCCAGAACCAGCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactgtgggatcat                                                                                                                                                                                            <  1:56206‑M1/251‑47 (MQ=255)                                                                         aaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGACCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccg                                                                                                                                                                              <  2:152310‑M1/251‑61 (MQ=255)                                                                          aaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGCGCAGATGCGCCAGCACCAATCAGCGCCAGACCGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatcttcatcataaactttgggatcatactgaagctccccgc                                                                                                                                                                             >  2:299128‑M1/1‑190 (MQ=255)                                                                               gTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgcc                                                                                                                                                                            >  1:92032‑M1/1‑185 (MQ=255)                                                                                   ttCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcct                                                                                                                                                                    >  1:320257‑M1/1‑181 (MQ=255)                                                                                   ttCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgccc                                                                                                                                                                    >  2:190233‑M1/1‑181 (MQ=255)                                                                                     ccatccaCCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcc                                                                                                                                                                  >  2:214085‑M1/1‑179 (MQ=255)                                                                                         ccaccaCTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgt                                                                                                                                                               >  1:29736‑M1/1‑175 (MQ=255)                                                                                                      aCCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcg                                                                                                                                                 >  1:203452‑M1/1‑162 (MQ=255)                                                                                                                             cAGAACCATCGCGGTTTGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGTAGCATATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctt                                                                                                                          <  1:130926‑M1/251‑113 (MQ=255)                                                                                                                                         ggTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaa                                                                                                              <  1:108504‑M1/251‑125 (MQ=255)                                                                                                                                                 aTTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctata                                                                                                                >  2:246778‑M1/1‑119 (MQ=255)                                                                                                                                                 aTTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctata                                                                                                                <  1:246778‑M1/241‑123 (MQ=255)                                                                                                                                                                tcttTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctg                                                                                         <  1:171519‑M1/249‑146 (MQ=255)                                                                                                                                                                   ttttCGTCCAAAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCGGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgct                                                                                       <  2:333675‑M1/248‑148 (MQ=255)                                                                                                                                                                             gAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagtt                                                                          <  2:233475‑M1/251‑161 (MQ=255)                                                                                                                                                                               aaCTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagtttt                                                                        >  2:110115‑M1/1‑89 (MQ=255)                                                                                                                                                                                   ggTCAAGTATCGGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtt                                                                     <  2:81030‑M1/250‑166 (MQ=255)                                                                                                                                                                                     acaAGTATCTGAGGAGCAGATGCGCCGGAACCAATCAGCGCAAGACCGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgttttt                                                                  <  2:11450‑M1/250‑169 (MQ=255)                                                                                                                                                                                           aTCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAGC‑TTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagcgataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaacc                                                             <  1:103176‑M1/250‑174 (MQ=255)                                                                                                                                                                                           aTCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaacc                                                             <  1:250026‑M1/250‑174 (MQ=255)                                                                                                                                                                                               gaggaaCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccga                                                           <  1:884‑M1/248‑176 (MQ=255)                                                                                                                                                                                                 ggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatac                                                      >  2:308620‑M1/1‑71 (MQ=255)                                                                                                                                                                                                                  cccaATCGGCGCCAGAACGGCATCCGACTGGCCGTATCTTA‑TCTTTTGTGTTCAcccggccgcttcgtcttacgatattcatcataaacgttgggatcatactgaagctccccgccagatgccgcctgtagacgtatcgcgcgaccttcgggaactaaagcccctttccagctatatagcgaagccaaacgaatacctgctgcttgtgcaagtgttgtttttgaaccgaaatacaaaagagcgtcagtttt                                     <  2:73931‑M1/250‑198 (MQ=255)                                                                                                                                                                                                                   ccAATCAGCGCCGGAACTGACGCCGCCAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggagcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatccaaaagagcgtcagtttta                                    <  2:239532‑M1/251‑199 (MQ=255)                                                                                                                                                                                                                        cAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcat                               <  1:50080‑M1/251‑204 (MQ=255)                                                                                                                                                                                                                         cgcgccAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCCcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatt                              <  1:190233‑M1/250‑205 (MQ=255)                                                                                                                                                                                                                          gcgcCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCCcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcattt                             <  1:33231‑M1/251‑206 (MQ=255)                                                                                                                                                                                                                                    ggCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctt                   >  2:41062‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                                          cGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctttattgt             <  1:55295‑M1/251‑222 (MQ=255)                                                                                                                                                                                                                                          cGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaag                                  >  2:283312‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                                          cGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaag                                  <  1:283312‑M1/230‑201 (MQ=255)                                                                                                                                                                                                                                             cAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctttattgtt            >  2:313178‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                              aGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctttattgttagt         <  1:151344‑M1/251‑226 (MQ=255)                                                                                                                                                                                                                                                      tCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaaaacctttattgttagtcataact  >  2:56600‑M1/1‑18 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    CATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑T‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTTCTTTGTCC  >  NC_000913/1639795‑1640288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑