Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,576	G→T	intergenic (‑154/‑152)	ibpA ← / → yidQ	small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,576	0	G	T	100.0%	212.4 / NA	56	intergenic (‑154/‑152)	ibpA/yidQ	small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/29);  total (27/29)

TTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGAGGG  >  NC_000913/3867331‑3867817                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ttACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGtt                                                                                                                                                                                                                                              >  1:661135/1‑251 (MQ=255)  tACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGttt                                                                                                                                                                                                                                             >  2:521412/1‑251 (MQ=255)   aCTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGttt                                                                                                                                                                                                                                             <  2:247323/250‑1 (MQ=255)      ctggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGACCACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATgg                                                                                                                                                                                                                                         >  1:711380/1‑251 (MQ=255)         gctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCt                                                                                                                                                                                                                                      >  1:667818/1‑251 (MQ=255)           cctggTTGTTTTCTAAGTGGTTAAACAAACGTTCAAATCTAATAGCAGAACGGTACAGCGGGGATAAATCAACGTGACGCATAAGCAAAAGCACCTGAAATCCGCGAGAATGTAAGAACCTCCACAAGTGACAGGTCAGGTAGCCAGAACACCCAGCAGGCGCGTCCTCATCGGCTACGATGGAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGTCTTTTTTTTTCAGTGTATGGTcgat                                                                                                                                                                                                                                    <  2:105491/250‑4 (MQ=255)              ggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTAtt                                                                                                                                                                                                                                   <  1:689842/249‑1 (MQ=255)              ggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTAtt                                                                                                                                                                                                                                   <  1:521412/249‑1 (MQ=255)                    tttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAAGGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGt                                                                                                                                                                                                                            <  1:386776‑M1/250‑2 (MQ=255)                      tCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTCGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtag                                                                                                                                                                                                                          <  1:105341‑M1/250‑4 (MQ=255)                       cTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGCCTTTTTTTGTCAGTTTTTGGTCTATTGCATAGtagtt                                                                                                                                                                                                                        >  2:48350‑M1/1‑246 (MQ=255)                       cTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGCAAAGCGTATATAAATCAAAGTTACGCATAAACAAAAGCTCCTGAAATCAGAGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCAAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGTTCTGGAAATGAAATTTTCAAACCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtaaat                                                                                                                                                                                                                        >  2:454367‑M1/1‑246 (MQ=255)                          aGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttg                                                                                                                                                                                                                     >  2:55267‑M1/1‑243 (MQ=255)                           gTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGGCCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgc                                                                                                                                                                                                                    >  1:624257‑M1/1‑242 (MQ=255)                             ggTTAAACAAACGGTAAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGGCCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACGTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgg                                                                                                                                                                                                                  <  2:624257‑M1/251‑12 (MQ=255)                                tAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcggga                                                                                                                                                                                                                <  2:529640‑M1/250‑14 (MQ=255)                                    caaacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgata                                                                                                                                                                                                           <  1:518259‑M1/251‑19 (MQ=255)                                     accctgTCAAATCCAATTGCAGAACGGGAAAGCGGGGATAAATCAAAGTTACGCATAATCCATAGCACCTGCAATCAGCGAGAATGTAAGACCTTCCACAATGAACAGGTCCGGTAGCCAGAACACCCATCCGGAGCGTCCTCATCGGCTACGAGGTAAAAATGGGTCTGGAAATGGACTTTTCAAGCCTTCTCAGCGGAATTTTTTTTTCAGTTTATGGTCTCTTCCATAgtagccttgcgggatgata                                                                                                                                                                                                           >  2:286051/6‑232 (MQ=255)                                     aaacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTCCGATGTAAAAATGGGACTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgata                                                                                                                                                                                                           >  2:358959‑M1/1‑232 (MQ=255)                                       acagtcaAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataatt                                                                                                                                                                                                        <  1:283809‑M1/248‑22 (MQ=255)                                       acGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataatt                                                                                                                                                                                                        <  1:306047‑M1/251‑22 (MQ=255)                                        cGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCATCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattg                                                                                                                                                                                                       <  2:224242‑M1/251‑23 (MQ=255)                                                  caatcgccgaacggtacAGCGGGGTTAAATCAAAGTTACGCATAATCAATAGCCCCTGAAATCAGCGCGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACAACCATCCGGCGCGCCCTCATCGGCTACGATGTAAAACAGGGACAGGAAATGAACTTTTCAAGCCTTACCAGCGGACTTTTTTTTTCAGTTTCTGGTCTATTGCATAGtagatttgcgggatgataattgcggattac                                                                                                                                                                                               >  2:167298‑M1/9‑219 (MQ=255)                                                      aGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaat                                                                                                                                                                                         <  1:55267‑M1/251‑37 (MQ=255)                                                      aGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaa                                                                                                                                                                                          <  1:572494‑M1/250‑36 (MQ=255)                                                        cAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAAGCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACCCCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataatggcggattgcagaaataa                                                                                                                                                                                       <  1:272255‑M1/251‑39 (MQ=255)                                                              ggTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaaggg                                                                                                                                                                                 >  1:33222‑M1/1‑207 (MQ=255)                                                                            aaaTCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctc                                                                                                                                                                    <  2:661135‑M1/250‑58 (MQ=255)                                                                              aTCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCCTAGttgatttgcgggttgatcattgtggatttcagaaattaaaagggcggagatgatcttcgc                                                                                                                                                                 >  2:1486‑M1/1‑191 (MQ=255)                                                                                    gTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgccctttt                                                                                                                                                           >  1:461291‑M1/1‑185 (MQ=255)                                                                                       aCGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttct                                                                                                                                                         <  2:842447‑M1/250‑69 (MQ=255)                                                                                             aaTCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagc                                                                                                                                                   <  1:157780‑M1/250‑75 (MQ=255)                                                                                                cAATAGCCCCTGAAATAAGCGAGAATGCAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTAATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatatccgcccttttcttatagcttct                                                                                                                                               >  2:132252‑M1/1‑173 (MQ=255)                                                                                                  aTAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACA‑CCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGGCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggcttgcagaaataaaaagggcggagatgatctccgcccttttcttcaagcctcttgc                                                                                                                                            >  2:840389‑M1/1‑170 (MQ=255)                                                                                                             aaTCAGCGAGAATGTAAGACCTTCCAAAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCAAAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggc                                                                                                                                  >  2:851295‑M1/1‑160 (MQ=255)                                                                                                                    gagaATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtggac                                                                                                                            >  2:684350‑M1/1‑153 (MQ=255)                                                                                                                             aGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttattcc                                                                                                                  >  1:191075‑M1/1‑144 (MQ=255)                                                                                                                                 cTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttccgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaaccccttatccggccc                                                                                                              >  2:23492‑M1/1‑140 (MQ=255)                                                                                                                                      acaATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaaccccttattcggcctacaa                                                                                                          >  2:678311‑M1/1‑135 (MQ=255)                                                                                                                                                    aggtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattca                                                                                           <  2:249979‑M1/251‑131 (MQ=255)                                                                                                                                                      gtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccgacctacaaaatcatgaaaattcaat                                                                                         >  2:808772‑M1/1‑119 (MQ=255)                                                                                                                                                          ccAGAACACCCATCAGGCGCGTCCTCACCGGCTACGATGTAAAAAGGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacat                                                                                     <  1:1486‑M1/251‑137 (MQ=255)                                                                                                                                                                cacCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAAGGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaag                                                                               <  1:258325‑M1/251‑143 (MQ=255)                                                                                                                                                                      acaGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttc                                                                         <  2:570607‑M1/250‑149 (MQ=255)                                                                                                                                                                          gcgcGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtag                                                                     <  1:426561‑M1/251‑153 (MQ=255)                                                                                                                                                                                           ccgATGTAAAAATGGGTCTGGAAATGAACTTTGCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATGGCATAGtagagttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcgtcgtgaacgccttatccggcctacaaaagcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagc                                                     <  2:228749‑M1/249‑169 (MQ=255)                                                                                                                                                                                             gATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttt                                                                                                                                                            >  2:176044‑M1/1‑80 (MQ=255)                                                                                                                                                                                             gATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttt                                                                                                                                                            <  1:176044‑M1/145‑66 (MQ=255)                                                                                                                                                                                                     aaTGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcagcaggcac                                          <  1:507912‑M1/251‑180 (MQ=255)                                                                                                                                                                                                         ggTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctc                                      >  1:848541‑M1/1‑68 (MQ=255)                                                                                                                                                                                                           tCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcc                                                                  >  2:163351‑M1/1‑66 (MQ=255)                                                                                                                                                                                                           tCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcc                                                                  <  1:163351‑M1/221‑156 (MQ=255)                                                                                                                                                                                                               gAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTAAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaaccccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggccagataagcgtagcgcatcaggcacgctcgcatgg                                >  2:609718‑M1/1‑62 (MQ=255)                                                                                                                                                                                                                      aCTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgc                         <  2:461291‑M1/251‑197 (MQ=255)                                                                                                                                                                                                                                           gACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggaggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgcc    <  2:1801‑M1/251‑218 (MQ=255)                                                                                                                                                                                                                                             cTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgccgc  >  1:85446‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGAGGG  >  NC_000913/3867331‑3867817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑