BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	99.0%	361.8 / NA	101	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (52/48); minor base T (0/1); total (52/49)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 101 total aligned reads displayed.

AACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCA  >  NC_000913/1337148‑1337637

aaCCCAAGTGTGGTGAAGGCCGCCGGCCTGCTGGCGAAAAAAACCCTTACTCCGGGCCTCAAGGGGCAAACATTGGGCCAAGCGTCGCTGGCACCGGGTTCGAAAATCGTTTCTGATTAATTGGCAAAAAGCGAA‑CTGGCCCCGTTTCCCCGCCGA‑CTGGGGTTTAACCTTGTGGGATTCG‑GTTGTACCCCCTGTAATGGTAACTCTTGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGa                                                                                                                                                                                                                                                 <  1:18370/251‑1 (MQ=255)

   ccAAGTGGGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACTCCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGAttt                                                                                                                                                                                                                                              <  2:687988/251‑1 (MQ=255)

     aaGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTa                                                                                                                                                                                                                                             >  2:171368/1‑250 (MQ=255)

        ggtgCTGATGGCCGCAGGATTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCg                                                                                                                                                                                                                                         <  2:62586/250‑1 (MQ=255)

                    cGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCggtgcggtgc                                                                                                                                                                                                                             >  1:634977/1‑251 (MQ=255)

                    cGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGCCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGAATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGATGCCCGATCCTAT‑CGAAACGGCAATCAAACCAGGCGCTTTAACAGTCggagcggtgc                                                                                                                                                                                                                             >  2:371401/1‑251 (MQ=255)

                      cAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTg                                                                                                                                                                                                                           >  1:839541/1‑251 (MQ=255)

                      cAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTg                                                                                                                                                                                                                           >  1:733074/1‑251 (MQ=255)

                       aGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGt                                                                                                                                                                                                                          >  1:545410/1‑251 (MQ=255)

                         gCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTcc                                                                                                                                                                                                                        >  1:679639/1‑251 (MQ=255)

                         gCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTcc                                                                                                                                                                                                                        <  2:411842/251‑1 (MQ=255)

                           ttGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCgg                                                                                                                                                                                                                      >  1:251954/1‑251 (MQ=255)

                            tGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCCTGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGCTTCTGATTATCTGGCAAAA‑GCGACACTGACACCGTATCTC‑GACGCACTGGTGTTTAACCTTGTGGGACACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAC‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGc                                                                                                                                                                                                                     >  2:876835/1‑251 (MQ=255)

                             gCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAC‑CGAAACGGCAATCAAAAAAGGCGTTTTAACCGCCGGTGCGGTGCTGTCCGGc                                                                                                                                                                                                                     >  2:668509/1‑250 (MQ=255)

                               tGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAc                                                                                                                                                                                                                  >  1:20441/1‑251 (MQ=255)

                                ggCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCaa                                                                                                                                                                                                                   <  1:694262/249‑1 (MQ=255)

                                      aaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACt                                                                                                                                                                                                           >  1:537195/1‑251 (MQ=255)

                                             gTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAATGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                                                                                                                                                                                    <  1:155212/251‑1 (MQ=255)

                                               aaCTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGcc                                                                                                                                                                                                  <  1:713142/251‑1 (MQ=255)

                                                     gggCCTCAAGCGGCAACCATGGGTCAAAGCGGCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCTTAACTTTGAAGGCCGTatc                                                                                                                                                                                             <  2:733074/250‑1 (MQ=255)

                                                     gggCCTCAAGCGGCAACCATGGGGCAAAGCGGCGCTGGCACCGGGTGCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTa                                                                                                                                                                                               <  2:838805/248‑1 (MQ=255)

                                                       gCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccat                                                                                                                                                                                          >  1:301986/1‑251 (MQ=255)

                                                         cTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                                                                                                                                                                                        >  1:453393/1‑251 (MQ=255)

                                                             aGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                                                                                                                                                                                    >  1:95137/1‑251 (MQ=255)

                                                             aGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                                                                                                                                                                                    >  1:137870/1‑251 (MQ=255)

                                                               cGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGt                                                                                                                                                                                  <  2:631711/251‑1 (MQ=255)

                                                                 gCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTa                                                                                                                                                                                <  2:137870/251‑1 (MQ=255)

                                                                   aaCCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaa                                                                                                                                                                               >  2:418963/1‑250 (MQ=255)

                                                                      cATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaact                                                                                                                                                                           >  1:275054/1‑251 (MQ=255)

                                                                          ggTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaact                                                                                                                                                                       <  1:599666/251‑1 (MQ=255)

                                                                           gTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg                                                                                                                                                                      >  1:709628/1‑251 (MQ=255)

                                                                            tCAAAGCGTAGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTCTCTGGCAAAC‑GCGAAACTGACCCCGTATCTC‑GACGCACTGGGGTTTACCCTTGTGGAATACG‑GTTGTACCACCTGTATTGGTAACGCTGAGCCGCTGCCCGATCCTAT‑CGAACAGGCAATCAAACAAGGCGATTTAACCGTAGGTGCGGTGCCGTCCGGCAACCGTCCCTATGACGGCCGAATCCATCCGATGGtacacactaatcgg                                                                                                                                                                     >  2:572414/1‑238 (MQ=255)

                                                                                  cGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGCTTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                               >  1:235780/1‑251 (MQ=255)

                                                                                  cGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                               <  1:672931/251‑1 (MQ=255)

                                                                                        tGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccg                                                                                                                                                         >  2:319617/1‑251 (MQ=255)

                                                                                        tGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccc                                                                                                                                                         >  2:22240/1‑250 (MQ=255)

                                                                                         ggCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgc                                                                                                                                                        >  2:654094/1‑251 (MQ=255)

                                                                                         ggCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgc                                                                                                                                                        >  1:446645/1‑251 (MQ=255)

                                                                                         ggCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgcc                                                                                                                                                          <  1:461626/249‑1 (MQ=255)

                                                                                                ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GTCGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggt                                                                                                                                                 >  1:849250/1‑251 (MQ=255)

                                                                                                 gTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtg                                                                                                                                                <  1:840621/251‑1 (MQ=255)

                                                                                                    cGAAAGTCGTTTCTGAATATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGtt                                                                                                                                             <  1:668509/251‑1 (MQ=255)

                                                                                                    cGAAAGTCGATTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGAATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGAAATGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTACGGCAACCGCAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGACGCCGCTGTTGGtt                                                                                                                                             >  2:593455/1‑251 (MQ=255)

                                                                                                     gAAAGTCGTTTCTGCTTATCTGGCAAAA‑GAGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGAAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGGGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATACGCTGGTTAAAACTAACTGGCTGGCCTGGCAGCCGCtggtggctg                                                                                                                                            >  2:417923/1‑248 (MQ=255)

                                                                                                     gAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                                                                                                                            >  2:572085/1‑251 (MQ=255)

                                                                                                           cGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATg                                                                                                                                      >  2:761677/1‑251 (MQ=255)

                                                                                                            gTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgc                                                                                                                                     <  1:744841/251‑1 (MQ=255)

                                                                                                                  gATTATCTGGCCAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGc                                                                                                                               >  2:293398/1‑251 (MQ=255)

                                                                                                                      aTCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCggg                                                                                                                            >  1:594675/1‑250 (MQ=255)

                                                                                                                          ggCAAAA‑GCGAAACTGACGCCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTCCCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGa                                                                                                                           <  2:92293/247‑1 (MQ=255)

                                                                                                                          ggCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGaaa                                                                                                                         <  1:884549/249‑1 (MQ=255)

                                                                                                                          ggCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAata                                                                                                                       <  1:184091/251‑1 (MQ=255)

                                                                                                                          ggCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAat                                                                                                                        >  2:172800/1‑250 (MQ=255)

                                                                                                                           gCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatat                                                                                                                      >  1:799430/1‑251 (MQ=255)

                                                                                                                               aa‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAat                                                                                                                  >  1:549302/1‑251 (MQ=255)

                                                                                                                                  gCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGGGGTTGCCTATGCGCTGGCGGGGAATATGAata                                                                                                                 >  2:633980/1‑250 (MQ=255)

                                                                                                                                   cGAAACTGACACCGTATCGC‑GAAGTACTGGGGTTTAACCTTGTGCGATTCGCGTT‑TACCCCATGTGTTGGTAAGTCTGGGCCGCTGCCCTATCC‑ATGCGAAACGGCAATCAAAAAAGGGGATTTAACCGTCGGTGCGGTGCTGTCCGGCAGCCGTAACTTTGAAGGCCGTATCCATCTGCTGGTTAAAACTAAGTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAatat                                                                                                                <  2:494803/250‑1 (MQ=255)

                                                                                                                                    gAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCa                                                                                                              <  2:537195/251‑1 (MQ=255)

                                                                                                                                       aCTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAAAATGAATATCAAcc                                                                                                           >  2:756243/1‑251 (MQ=255)

                                                                                                                                         tGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTg                                                                                                         >  1:25413/1‑251 (MQ=255)

                                                                                                                                          gACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATCCG‑GTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTg                                                                                                         <  2:251954/250‑1 (MQ=255)

                                                                                                                                           acacCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGc                                                                                                       >  2:27421/1‑251 (MQ=255)

                                                                                                                                           acacCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGAACCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGCATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGACTATGAGCTGGCGGGAAATATTAATATCAACCTGGc                                                                                                       >  1:736848/1‑251 (MQ=255)

                                                                                                                                             acCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCtt                                                                                                     >  1:196008/1‑251 (MQ=255)

                                                                                                                                                      c‑gacgaACTGGGTTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGACGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCt                                                                                             <  1:415453/250‑1 (MQ=255)

                                                                                                                                                      c‑gacgaACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGGAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTa                                                                                            <  1:524061/251‑1 (MQ=255)

                                                                                                                                                      c‑gacgaACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCt                                                                                             >  1:63997/1‑250 (MQ=255)

                                                                                                                                                       cggcgaACTTGGGTTTAACCTTGTGGGCTACG‑GTGGGACCACCTGTAGTGGAAACTCTGGGCCGCTGCCGGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCGACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCGATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGTGcc                                                                                              <  2:206774/246‑1 (MQ=255)

                                                                                                                                                        gacgtACTGGGGTTTAACCTTGAGGCAGACG‑GTTGTACCACCTGCATTGGCAACTCTGGGCCGCTGCCCGATCCTAT‑CGACACGGCAATCAAAAACGGCGATTTAACCGTCGGTGCGGAGCAGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGGTAAAACTAACTGGCTGGCCTCGACGCCGCGGGTGGTGGCCTATTCGCTGGCGGGTCATCGGAATATCAACCTGCCTTCTTAGcgtac                                                                                           >  2:887693/1‑247 (MQ=255)

                                                                                                                                                         acgaACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATc                                                                                          <  1:572085/251‑1 (MQ=255)

                                                                                                                                                              cTGGGGTTTAACCTTGTGGGATACG‑GTTGTCCCACCTGTATTGGAAACTCTGGGCAGCTGCCCGATCCTAT‑CGAAACGCCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCAGCCGCTGGTGGTTGCCTATGCGCCGGAGGGACATAAGAAAATCTACAAGGCCTCTGACCCTATCGGccc                                                                                     >  2:454573/1‑250 (MQ=255)

                                                                                                                                                                ggggTTTAACCTTGTGGGATACG‑GTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATg                                                                                   <  1:491341/251‑1 (MQ=255)

                                                                                                                                                                ggggTTTAACCGTGGGGGATACG‑GTTGTACCACCTGTATATGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACTGCAATCAAAAAAGGCGAGTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAGCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCAt                                                                                    <  2:235780/250‑1 (MQ=255)

                                                                                                                                                                 gggTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGa                                                                                  <  1:604233/251‑1 (MQ=255)

                                                                                                                                                                      tAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGAGCATAT‑CGAAACGGCAATAAAAAAAGGCGATTTAACCGTCGGAGCGGCGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGACGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGACTATCAACCTGGCttttcatccgttacgccaaggtcgc                                                                              >  2:306551/1‑227 (MQ=255)

                                                                                                                                                                             gtgGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGaa                                                                                                                          <  1:235091/199‑1 (MQ=255)

                                                                                                                                                                             gtgGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCg                                                                       <  1:489351/250‑1 (MQ=255)

                                                                                                                                                                                ggATTCG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATcc                                                                   <  2:535656/251‑1 (MQ=255)

                                                                                                                                                                                ggATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATcc                                                                   <  1:171368/251‑1 (MQ=255)

                                                                                                                                                                                 gaaccG‑GTTGTACCACCTGTAGTGGTAAATCTGGGTCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGACTATCGGCCATGATCGCAAAGGCGATCCg                                                                  <  2:701285/247‑1 (MQ=255)

                                                                                                                                                                                           gtcccaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTATCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGTCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCt                                                          <  2:673504/247‑1 (MQ=255)

                                                                                                                                                                                           gTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGGGGTCCGGTTTATCTg                                                         >  2:788008/1‑251 (MQ=255)

                                                                                                                                                                                             accaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAA‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGGAATATGAATATCAACCTGGATTCTGAGCCTACCGGCCAAGATCGCAAAGGCCATCCGGGTTATccga                                                        >  2:739862/1‑247 (MQ=255)

                                                                                                                                                                                                    gTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGatat                                                 <  1:655305/250‑1 (MQ=255)

                                                                                                                                                                                                      aTTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCGCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGCAATATGCATATCAAACTGGCTTCTGAGCCTATCGGCACTGCACGCAAAGGCGGACCGGATTATCTGTAAGatttatg                                              >  2:522230/1‑246 (MQ=255)

                                                                                                                                                                                                             aCTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCg                                       <  2:177717/251‑1 (MQ=255)

                                                                                                                                                                                                                ctGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATTCGGCCATCGGcc                                    >  2:103111/1‑250 (MQ=255)

                                                                                                                                                                                                                ctGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCgg                                      <  1:8258/249‑1 (MQ=255)

                                                                                                                                                                                                                ctGGGCCGCAGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGCTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTAACGGCCATGCTCGCAAAAGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGc                                     >  2:805081/1‑250 (MQ=255)

                                                                                                                                                                                                                cggggCCGCGGCCCGATCCGAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGc                                     <  2:634977/248‑1 (MQ=255)

                                                                                                                                                                                                                  gggCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcaca                                  <  1:400192/251‑1 (MQ=255)

                                                                                                                                                                                                                         tGCCCGATCATAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAAtt                           >  1:618730/1‑251 (MQ=255)

                                                                                                                                                                                                                          gCCCGATCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAAt                            <  1:119034/249‑1 (MQ=255)

                                                                                                                                                                                                                             cgttCCTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGccc                       <  2:453393/248‑1 (MQ=255)

                                                                                                                                                                                                                                 ccTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTg                    <  1:756243/250‑1 (MQ=255)

                                                                                                                                                                                                                                 ccTAT‑CGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGACCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCTGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTg                    <  2:709628/250‑1 (MQ=255)

                                                                                                                                                                                                                                                  tCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTcc   >  1:51013/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCca  <  2:409466/251‑1 (MQ=255)

                                                                                                                                                                                                                                                   cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCca  <  1:761677/251‑1 (MQ=255)

                                                                                                                                                                                                                                                   cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCGGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTcc   <  2:190054/250‑1 (MQ=255)

AACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAA‑GCGAAACTGACACCGTATCTC‑GACGAACTGGGGTTTAACCTTGTGGGATACG‑GTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAT‑CGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCA  >  NC_000913/1337148‑1337637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`