Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,861	A→G	intergenic (+79/+6)	C0293 → / ← ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,861	0	A	G	100.0%	122.7 / NA	34	intergenic (+79/+6)	C0293/ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

TATGTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTAT  >  NC_000913/1196625‑1197075                                                                                                                                                                                                                                             |                                                                                                                                                                                                                       taTGTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacg                                                                                                                                                                                                          >  1:201539‑M1/1‑242 (MQ=255)      aTTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAcaatcaacgatta                                                                                                                                                                                                      <  1:784165‑M1/250‑14 (MQ=255)               tattaGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgct                                                                                                                                                                                             <  1:743999‑M1/250‑23 (MQ=255)                                aTTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgttt                                                                                                                                                                           >  1:270353‑M1/1‑211 (MQ=255)                                   aCCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttac                                                                                                                                                                         >  1:576401‑M1/1‑208 (MQ=255)                                       cTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggat                                                                                                                                                                    >  1:687753‑M1/1‑204 (MQ=255)                                         ccTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgt                                                                                                                                                                  >  1:775277‑M1/1‑202 (MQ=255)                                                       aaaTATTGTTAAAGTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACATAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaatt                                                                                                                                                    >  1:302213‑M1/1‑188 (MQ=255)                                                         atatTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACCGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattct                                                                                                                                                  >  2:462071‑M1/1‑186 (MQ=255)                                                                    ttGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaa                                                                                                                                        >  1:484316‑M1/1‑175 (MQ=255)                                                                                 aTCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGCACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagtt                                                                                                                           <  1:814412‑M1/250‑89 (MQ=255)                                                                                     actactCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggc                                                                                                                      >  1:708018‑M1/1‑158 (MQ=255)                                                                                     actactCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggc                                                                                                                      >  1:1676‑M1/1‑158 (MQ=255)                                                                                        actCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggg                                                                                                                   >  1:706609‑M1/1‑155 (MQ=255)                                                                                                   aCTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcc                                                                                                        <  1:447408‑M1/251‑108 (MQ=255)                                                                                                                 agaaaacaggCAGTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaacggcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagaccgccagttacggcgggaaacctcatcctatgggagaacaat                                                                                          <  2:270353‑M1/247‑122 (MQ=255)                                                                                                                  gCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggagaactcacccctctgggtaaccc                                                                                           >  2:172825‑M1/1‑129 (MQ=255)                                                                                                                     aaCAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaaactcatcctatggggggaaaatgaat                                                                                      >  1:462299‑M1/1‑126 (MQ=255)                                                                                                                               gggTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgacagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaatt                                                                             <  2:108291‑M1/250‑135 (MQ=255)                                                                                                                                      aTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaaactcatcctatgggggaacaatgaataatgaaattgcggggtt                                                                     >  1:157726‑M1/1‑109 (MQ=255)                                                                                                                                                    aagGTACATACGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGGAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagta                                                       <  2:687753‑M1/250‑157 (MQ=255)                                                                                                                                                     agggacaggcggaatttAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTTAGACTGTTTAACCGAGGGAATTATTGTCGTTccatcagagattaaactgcgcttcggcgaagttttcgtttacaggaggttgaaagggaaaattctggggcaaaaaaatgccgccagttacggcgtgaaacctcatcctatgggtgaacaatgaataatgaaattgcggggttatgatctcccagtat                                                      <  2:340913‑M1/243‑158 (MQ=255)                                                                                                                                                           aTGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccat                                                <  2:457952‑M1/251‑164 (MQ=255)                                                                                                                                                             gCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaaactcatcctatgggagaacaatgaataatgaaaatgcggggttatcatctccccggatatccatac                                              >  1:162835‑M1/1‑86 (MQ=255)                                                                                                                                                                      aaaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaata                                      <  2:230006‑M1/250‑174 (MQ=255)                                                                                                                                                                         aaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggt                                  <  1:778944‑M1/251‑178 (MQ=255)                                                                                                                                                                             gCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggggaaactcatcctattggggaacaatgaaaaatgaaatttcgggggtatcatctcccactaaattcatactaacaataaggttatt                              >  1:388793‑M1/1‑70 (MQ=255)                                                                                                                                                                              ccggtagctcggccccttcaCATCCATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATGGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttattt                             <  2:576401‑M1/242‑183 (MQ=255)                                                                                                                                                                                      gcgGCACCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaacc                     <  2:706609‑M1/251‑191 (MQ=255)                                                                                                                                                                                         gCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggaaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccagg                  <  1:172825‑M1/251‑194 (MQ=255)                                                                                                                                                                                                    tacatCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggggaacaatgaataattaaaaatcgggggtatcatctccccgcatatccataccaacaaaaagggtatttattcaacccagcctaaacattt       >  1:418757‑M1/1‑47 (MQ=255)                                                                                                                                                                                                           ttAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttt  <  2:484316‑M1/249‑210 (MQ=255)                                                                                                                                                                                                           ttAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgtt   <  1:190156‑M1/248‑209 (MQ=255)                                                                                                                                                                                                           ttAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgt    <  2:388793‑M1/247‑208 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                       TATGTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTAT  >  NC_000913/1196625‑1197075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑