Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,027	T→G	intergenic (‑36/‑323)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,027	0	T	G	100.0%	139.5 / NA	39	intergenic (‑36/‑323)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/21);  total (18/21)

ACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTA  >  NC_000913/4312798‑4313246                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            aCTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGa                                                                                                                                                                                                         >  2:175436/1‑250 (MQ=255)    atcatcGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGaaaa                                                                                                                                                                                                      <  1:83940/250‑1 (MQ=255)     tcatcGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCCCTAGATCACCACAGCAAGAACAGaaa                                                                                                                                                                                                       >  2:59408/1‑248 (MQ=255)      catcGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGaaa                                                                                                                                                                                                       >  2:372085/1‑247 (MQ=255)          gCTTCAGATACCGCCGTCGATACTGAGACATATGTAATTGCGGGGGCACAACTCAGTTTACCGGGTGTTAGTAGCCACTCCACGATGCGGCTGTCATTTTCTGTCATTCCTTCCTGCTTCATTCTCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAAGCAAACGCTTACTGGCTCATCACGTTCTCATGGCCG‑TGAAGTGAAGACGCGACTAGATCACAACATCAATAACAGTAAAGATGCt                                                                                                                                                                                                <  2:58840/250‑1 (MQ=255)              cAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCg                                                                                                                                                                                            <  1:77691/250‑1 (MQ=255)                 atcccGCCAGTGCTTCTGCGACATCTTTCATTGCGGGTGCACAACTCAGGCTACCAGGTTTGAGTAACCACTCCACGAAGCGGCTTTAATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCCCTAACCCTATACCGTTCGGAAGCGCTGAATCAACCTCTGACTGGCTCATCACGTGCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCTCCACAGCAAGAACAGAAAAGATGCTGGCGACtt                                                                                                                                                                                        <  2:298553/248‑1 (MQ=255)                 aTACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGTAAGAACAGAAAAGATGCTGGCGACtt                                                                                                                                                                                        >  1:120560/1‑251 (MQ=255)                 aTACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACtt                                                                                                                                                                                        <  2:322484/251‑1 (MQ=255)                      gCCAGAGCTTCGGCGACATCTTTAATTGCGGGTGCACAAAGCAGGTTACCGGGTTTGAGTAACAACTCCACGATGCGGCTGTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑GGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACaa                                                                                                                                                                                   <  2:242511/251‑1 (MQ=255)                               tCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaata                                                                                                                                                                            <  1:381725/249‑1 (MQ=255)                                           ttAATTGCGGGTTCACAACTTAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTGCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGt                                                                                                                                                               <  2:78278/250‑1 (MQ=255)                                              aTTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTACTTCATGCTTCATTCGCAGGCAAGGCGCTAACCCTATACAGTTCGGAAGCGCTGAGTCAAACTCTGACCGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGATGCCACTAGATCACCACAGCAAGAACAGAAAAGATGTTGGCGACTTCACAAAAACATACAATTTATAAAGtttat                                                                                                                                                           >  2:14949/1‑251 (MQ=255)                                                tGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttatt                                                                                                                                                          <  1:417143/250‑1 (MQ=255)                                                          caACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAAc                                                                                                                                               <  1:384287/251‑1 (MQ=255)                                                            aCTCAGGTTACCGGGTTTGAGTACCCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TTAATTGAAGACGCCACTAGATCACCACAGCAATAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACaa                                                                                                                                             <  2:174610/251‑1 (MQ=255)                                                                aGGTTACCTGGTTTGAGTAACCTCTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAATGCGCTAACCCTATTCCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCAGCACGTTCTCTGGTCCT‑TGAAGTGAAGACTCCACTAGATCACCACAGCAAGAACAAAAAAGATGCTGTCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTGATTGTTAACAATGGc                                                                                                                                         <  2:141506/251‑1 (MQ=255)                                                                 ggTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAACTAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCg                                                                                                                                        >  2:168696/1‑251 (MQ=255)                                                                         ggTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCtt                                                                                                                                <  2:376829/251‑1 (MQ=255)                                                                               aGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtt                                                                                                                          >  1:354644/1‑251 (MQ=255)                                                                               aGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtt                                                                                                                          >  2:325743/1‑251 (MQ=255)                                                                                         ccACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAt                                                                                                                 <  1:175436/250‑1 (MQ=255)                                                                                           aCGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATtgtg                                                                                                                                                  >  1:299302/1‑215 (MQ=255)                                                                                           aCGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATtgtg                                                                                                                                                  <  2:299302/215‑1 (MQ=255)                                                                                                gCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAAtt                                                                                                           >  1:280407/1‑249 (MQ=255)                                                                                                   gCTTTCATTTTCTGTTATTCCTTCCTGCTTCATTCGCAGGTAAGGCGTTAACCCTATCCCGTTCGGCAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGCTCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTAACAACAAAATAAAATTTATAAAGATTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCCTTTAATTAGttt                                                                                                      >  2:114966/1‑251 (MQ=255)                                                                                                   gCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCACTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGttt                                                                                                      <  1:230640/251‑1 (MQ=255)                                                                                                      gtCATTGTCTGTCATTCCTGCCTGCTTCAGTCGCAGGTAAGGCGCTAACCCTATACCTTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGACCG‑TGAATTGAATACGCCACTAGATCACCACAGCGAGAACAGAAAAGATGCTTGCGACTTGACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAAGGGCGAGTGGGTGAAAATTTTATTTCATTTAATTATTTTa                                                                                                     <  2:96178/248‑1 (MQ=255)                                                                                                          ttttCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAAc                                                                                                >  1:262409/1‑250 (MQ=255)                                                                                                                   aTTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAt                                                                                      >  2:391844/1‑251 (MQ=255)                                                                                                                     tccttcctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAtt                                                                                     <  2:206214/250‑1 (MQ=255)                                                                                                                          cctgttgcATTCGCAGTTCAGGCGCTCACCCTATACCTTTCGGAAGCGCTGAATCAAACGCTGACTGGCTCAGCACGATCTCTTGTCCTATGA‑TTGAGGAAGCCACGAGATATACACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtttt                                                                               <  2:422988/244‑1 (MQ=255)                                                                                                                                     cGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt                                                                    <  1:314254/251‑1 (MQ=255)                                                                                                                                              ggCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTCGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatgat                                                             >  2:250347/1‑249 (MQ=255)                                                                                                                                                          ataCCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGAt                                               >  1:241511/1‑251 (MQ=255)                                                                                                                                                                  cGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAgtg                                       >  1:160358/1‑251 (MQ=255)                                                                                                                                                                                                    aCGTTATCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTAATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAActct     >  2:257871/1‑251 (MQ=255)                                                                                                                                                                                                       ttCTCTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGt   >  1:97515/1‑250 (MQ=255)                                                                                                                                                                                                        tctcTTGTCCT‑TGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTa  <  1:372085/250‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            ACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCT‑TGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTA  >  NC_000913/4312798‑4313246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑