| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,867,576 | G→T | intergenic (‑154/‑152) | ibpA ← / → yidQ | small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,867,576 | 0 | G | T | 97.1% | 129.6 / ‑2.5 | 35 | intergenic (‑154/‑152) | ibpA/yidQ | small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (21/13); minor base . (1/0); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
GGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGA > NC_000913/3867338‑3867814 | ggctttggTTTTTTTCTAAGTTGTTAAACAAACGGTCTAATCCAAGAGCAGAGCGGTGAAGCGGGGATAAATCAAATTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGTCAGTTCAGGTAGCCAGAACTTCCAGAAGGCGCGTCCTCATCGGCTACTATGTAAAAATGGGTCTGAAAATGAGCTTTGCAAGCCTTATCAGCGTACTTTTTGTTTCAGTTTATGGt < 2:394550/250‑1 (MQ=255)ggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTAAAATCAAATAGCAGAACGGTAAAGAGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCCAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTc > 2:327860/1‑251 (MQ=255) ggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTAt < 1:121698/248‑1 (MQ=255) ggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTGCCACAATGGACAGGTCATGTAGCCAGAACGCCCATCAGGCGCGTCCTCGTCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTg < 1:393232/250‑1 (MQ=255) gttgttTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTT‑CAGTTTATGGTCTATTGCAt > 1:359624/1‑251 (MQ=255) aaGTGGTTAAACAAACGGTCAAATCCAAGAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAACCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAATTGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGTTCTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagat < 2:407822‑M1/249‑6 (MQ=255) aaacGGTAAAATCCAATAGCAGAAGGGTACAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTACTGAAATCAGCGAGAATGTCAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGACGCGTCCTCCTCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAACCTTATCAGCGGACTTTTTTTTTCAGTTTATGTTTTATTGCAGAGtagtctttcgggttgtta > 2:90462‑M1/1‑232 (MQ=255) aTAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcaga > 2:196790‑M1/1‑217 (MQ=255) gCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcgg > 1:141897‑M1/1‑201 (MQ=255) aaaTTAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTTGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctcc < 1:379746‑M1/251‑59 (MQ=255) aaaTCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctcc < 2:196882‑M1/251‑59 (MQ=255) gTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgccctttt > 1:50362‑M1/1‑185 (MQ=255) cATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGgagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctacgcccttttcttat > 1:205301‑M1/1‑179 (MQ=255) cAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttct < 2:50362‑M1/251‑79 (MQ=255) aGCTCCTGAAATCAGCGAGAATGGAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGGCCTCATCGGCTACGATGAAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGGCTATTGCATAGtagatttgcgggatgataattgcggcttgcagaaataaaaagggcggagatgctctccgccctgttcttatagcttcttgcc > 1:236891‑M1/1‑169 (MQ=255) gCGAGAATGTAAGACCTTCCACAATGGCCAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGAATTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtga > 1:341294‑M1/1‑155 (MQ=255) gagaATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacg > 1:173836‑M1/1‑153 (MQ=255) agaATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgc > 1:146805‑M1/1‑152 (MQ=255) aTGTAAGACCGTCCACAATGGACAGGTCAGGTAGCCAGAACACCCAGCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGATCTTTTCAAGCCTTATCAGCGGACTGTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgcctt < 2:36213‑M1/251‑103 (MQ=255) aGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGAGGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccg < 2:189352‑M1/251‑108 (MQ=255) tGGACAGGTCAGGGAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagacgatctccgcccttttcttatagattcttgcaggatgcggcgtgaaccccttttccggcctacaaaatcc > 2:447875‑M1/1‑131 (MQ=255) tcaggtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaatt > 1:172143‑M1/1‑123 (MQ=255) gcgcGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtaaatttgcgggatgctaattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaaagccttatccgtctttcacaataatcaaaattcaataaagttaaaaattttcct > 2:325466‑M1/1‑99 (MQ=255) cgTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcc > 1:156507‑M1/1‑96 (MQ=255) cTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaatgccttatccggcctacaaaatcatgaaaattcaat < 1:295452‑M1/224‑133 (MQ=255) tCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggttgataattgcggattgcagaaataaaaagagcggagatgatctccgcccttttcttatagcatcttgccggatgcggcgtgaacgccttatcctgccttcaaaatcatggaaattaaataaattgcaaacatttctgaaccctgct > 2:263294‑M1/1‑91 (MQ=255) aTCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaataatgaaaattcaatacattgcaagattttcttaagcctgata > 1:159575‑M1/1‑89 (MQ=255) gATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgca < 2:115820‑M1/251‑172 (MQ=255) gTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatc > 1:353101‑M1/1‑77 (MQ=255) gAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatgg > 1:76939‑M1/1‑62 (MQ=255) ccTTATCAGCGGACTTTTGTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagaggatctccgcccttttcttatagcttctttccggatgcggtgtgaacgccttatccggcctacaaaatcatgaaaattcaatacatggcaagattttcgtagtcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaata < 2:383096‑M1/250‑206 (MQ=255) tATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcg > 1:207808‑M1/1‑42 (MQ=255) cGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggttgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgtaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcatgcacgctcgcatggttagcgccattaaatatcgttattcg > 2:52646‑M1/1‑36 (MQ=255) cGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcttaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcg < 1:388918‑M1/251‑216 (MQ=255) ggACTTTTTTTTTCAGTTTATGGTCTATTGAATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggcgatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatcccgcctacaaaatcatgaaaattcaatacattgcaagattttcgtcggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgc > 1:126472‑M1/1‑35 (MQ=255) | GGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGA > NC_000913/3867338‑3867814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |