Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,790,153 C→T P58S (CCC→TCC)  lhgD → L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,790,1530CT97.7% 146.9 / ‑5.2 44P58S (CCC→TCC) lhgDL‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/17);  minor base A (1/0);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATT  >  NC_000913/2789907‑2790392
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
aggcccaacgaaaagctctattgattacgttgggcctaaacgcatggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGGTTGATGTCTATCCGGACGACACCCTTGCGTTGCTGGAACAAGGGTCCGCCCCGGCCTGTC‑ACAAGACGGGACACAACAGCGGCGAGAGCCAAGCCAGGGGCGATGACAcgccccg                                                                                                                                                                                                                                             >  2:172999‑M2/57‑249 (MQ=255)
      aacgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGACATGCAACTGATTGATGTCTATCCGGACGCACGCCTTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCCTGGCGGGGGCCATTACACTACCGGCAGCCt                                                                                                                                                                                                                                       >  2:240375‑M2/51‑251 (MQ=255)
           aaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAgg                                                                                                                                                                                                                                  >  1:358589‑M2/46‑251 (MQ=255)
                    tggtttacgtggggcctaaccgcagggagactccccGCGCAAAGGAATTGAGCGGATGTAGGATTTTGTGATTATGGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGttt                                                                                                                                                                                                                          <  2:136458‑M2/214‑1 (MQ=255)
                           cgtgtggcctaaccgcggggagactccccGAGCAAAGGATTGGAGCGGATGTATGATTTTGTGATTATGGGGGGCGGCATCATCGGCATGACGACCGCCATGCAACTGATTGATGTCTCCCCGGACGCCCGCATTGCGTGGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGc                                                                                                                                                                                                                  <  2:10684‑M2/222‑1 (MQ=255)
                                  aataaagcgatgtggcatcccctcgcAAAGGAATTAGGCGGATGTATAATTTTGGGTTTATTGGCGGAGGCACAATCGGCATGCCGCCCGCCATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGTCC‑GTCCACCCGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCGATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAc                                                                                                                                                                                                           <  2:430624/228‑1 (MQ=255)
                                          cagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccac                                                                                                                                                                                                   >  1:397860‑M2/15‑251 (MQ=255)
                                                    ccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCttt                                                                                                                                                                                         <  1:424997‑M2/247‑1 (MQ=255)
                                                                gAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc                                                                                                                                                                             >  1:441398/1‑251 (MQ=255)
                                                                       gCGGAGGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTc                                                                                                                                                                      <  1:186736/251‑1 (MQ=255)
                                                                                 gATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACa                                                                                                                                                             >  1:92236/1‑250 (MQ=255)
                                                                                     ttGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGc                                                                                                                                                        >  1:375012/1‑251 (MQ=255)
                                                                                                ggcggcggcATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCt                                                                                                                                             <  1:247818/251‑1 (MQ=255)
                                                                                                         atcatcGGCATGTAGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCAc                                                                                                                                    >  2:392573/1‑251 (MQ=255)
                                                                                                                    gTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCg                                                                                                                         >  2:93810/1‑251 (MQ=255)
                                                                                                                                gCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACgg                                                                                                              >  1:387305/1‑250 (MQ=255)
                                                                                                                                   aCTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgc                                                                                                          >  1:144308/1‑251 (MQ=255)
                                                                                                                                       attgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcgcgc                                                                                                      >  1:164213/1‑251 (MQ=255)
                                                                                                                                          gaggTCTACCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGCCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCGGAAGGCGCAGGTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCt                                                                                                    <  2:397860/247‑1 (MQ=255)
                                                                                                                                           atGTCTATCCGGACGCCCGCATTGCGTTTCTGGAAAAAGAGTCGGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTACGGCAGCCTGAAGGCGCAGTGTTGCCTGGCGGGAAACCGCGCCAACAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCGCGAAATGGAACGGATGCGCGCCtt                                                                                                   >  2:36948/1‑250 (MQ=255)
                                                                                                                                                tATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATggg                                                                                              >  1:19687/1‑250 (MQ=255)
                                                                                                                                                       aCGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcac                                                                                       >  2:173711/1‑250 (MQ=255)
                                                                                                                                                            cGCATTGCGTTGCTGGAAAAAGAGCCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAgtggc                                                                                 <  1:290359/251‑1 (MQ=255)
                                                                                                                                                              cATTGCGTTGGTGGACAAAGCGTCCGCCCCCGCCTGTC‑ACCAGACGGCCAACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCACAGTTTTGCCTGGCGGGAAACCGCGCCCCCAACGCCTTTTGCGATCAAAACGGCATTCGCTACGACCACTGCGGCAAGCTGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGCACGCCCAgtggcg                                                                                >  2:336411/1‑250 (MQ=255)
                                                                                                                                                                    gTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                          >  2:239088/1‑250 (MQ=255)
                                                                                                                                                                         tGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcac                                                                                       >  1:226084/1‑232 (MQ=255)
                                                                                                                                                                               aaGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGaa                                                              <  1:403777/251‑1 (MQ=255)
                                                                                                                                                                                   gTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGc                                                          >  1:157832/1‑251 (MQ=255)
                                                                                                                                                                                    tCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCt                                                         >  1:302505/1‑251 (MQ=255)
                                                                                                                                                                                        ccccGGACTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAAc                                                     >  1:56800/1‑251 (MQ=255)
                                                                                                                                                                                              ccTGTC‑CCCAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                               <  1:184096/251‑1 (MQ=255)
                                                                                                                                                                                                     ccaaggcGGGCCCCAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGGTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTg                                         <  2:428794/245‑1 (MQ=255)
                                                                                                                                                                                                      ccAGACGGGCCACAACAGCGGAGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAACGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGATGGTTGCCAAGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAAAGCAAAGAGGCGAACGGTATCGAGCGCGAATGGCTTATGGCCGACGAACTgg                                        >  2:195903/1‑250 (MQ=255)
                                                                                                                                                                                                       cAGACGGCTCACAACAGCGGCGTGATCCATGACGGAGTCTATTACACGTCCGGCAGCCTGAACCCGCAGTTCTGCCTTGCGGGAAACCGCGCCACCAAAGCCTTTTGCGTTCAAAACGGCATTCGCTACGACAACTACCGCAAGATCCTGTTTGCCACGTACGATCCCGAAATGGAACGGATACGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGGGGGAATGGCTTAACGCCGACGAACTgcg                                       >  2:161940/1‑251 (MQ=255)
                                                                                                                                                                                                            gggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgagc                                  <  2:358589/251‑1 (MQ=255)
                                                                                                                                                                                                                  caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                            <  1:93810/251‑1 (MQ=255)
                                                                                                                                                                                                                  caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                            <  2:375012/251‑1 (MQ=255)
                                                                                                                                                                                                                    acaGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGCTGCGCGCCTTCTGGGAACGCACAGTGGCGAACGGGATCGAGCGCGAATGGCTTAACGCCGACGAACGGCGCGAGCGCGAACCg                          >  2:59399/1‑251 (MQ=255)
                                                                                                                                                                                                                          gcgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATc                    <  1:80633/251‑1 (MQ=255)
                                                                                                                                                                                                                              gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGCATCGAGCGCGAATGGCTTAACGCCGACGAACCGCGCGAGCGCGAACCGAATATCaacg                >  2:224358/1‑248 (MQ=255)
                                                                                                                                                                                                                                   aTGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTc           <  1:173711/251‑1 (MQ=255)
                                                                                                                                                                                                                                           gTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAt   >  1:393637/1‑251 (MQ=255)
                                                                                                                                                                                                                                           gTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAt   >  1:130555/1‑251 (MQ=255)
                                                                                                                                                                                                                                            tCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt  >  2:207680/1‑251 (MQ=255)
                                                                                                                                                                                                                                               aTTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCCATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGTCGGCAtt  <  1:195903/248‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTC‑ACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATT  >  NC_000913/2789907‑2790392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑