Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,586	G→A	Q249Q (CAG→CAA)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,586	0	G	A	100.0%	164.3 / NA	47	Q249Q (CAG→CAA)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/27);  total (20/27)

CGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCA  >  NC_000913/2251340‑2251829                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cGTGGAAGATAACTCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGCTGCGTTCCGCTGGATCATGCAAgacg                                                                                                                                                                                                                                                 >  2:370238/1‑251 (MQ=255)  gTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAACATCGTTCCTGATGCGTTCCGCTGGATCATGCAAgacg                                                                                                                                                                                                                                                 >  1:241710/1‑250 (MQ=255)                            cggCATTGATCCCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATcc                                                                                                                                                                                                                      <  2:202487/249‑1 (MQ=255)                            cTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATcc                                                                                                                                                                                                                      >  2:347189/1‑251 (MQ=255)                              gCATTGATCCCTGCCATGCTTTCGTTGCCGGGTATGATTTGCGTACTCCACACGAATGCGAGAAAACATCCGCGGAATTTGCCCGTACTGTCGGCTTTAATTATCTGCGCGGGATGCACCTTAACTATGCGAAAAGCACCTTTGGCAGTCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc                                                                                                                                                                                                                    <  2:954/251‑1 (MQ=255)                              gCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTc                                                                                                                                                                                                                    <  1:320627/251‑1 (MQ=255)                                                                                        cGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCCCCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACaaa                                                                                                                                                          <  2:57839/251‑1 (MQ=255)                                                                                         gagaAAACATTCGCGGATTTTGCCCGTACTTTCGGCATTAAGTATCTGCGCTGGATGCCCCTGAACGATGCGAAAAGCGCCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGCGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAAc                                                                                                                                                         <  2:138955/251‑1 (MQ=255)                                                                                         gagaAAACATTCGCGGATTTTGCACGTACTGTCGGCTGTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGCAAGCGCAACAAAc                                                                                                                                                         >  2:296445/1‑251 (MQ=255)                                                                                                 aTTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAg                                                                                                                                                 <  1:323569/251‑1 (MQ=255)                                                                                                    cgcgGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCgg                                                                                                                                              >  2:403665/1‑251 (MQ=255)                                                                                                     gcgGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGt                                                                                                                                             <  1:427474/251‑1 (MQ=255)                                                                                                              gCCCGTACTGTCGGCTTTAAGGATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGGAAGCGCAACAAACTGAAAAAGCGGTAGCCTGa                                                                                                                                      >  2:288909/1‑249 (MQ=255)                                                                                                                       gTCGGCTTTAAATATCTGCGCGGGATGCCCCTTAACGAGGCGAAAAGCACCTTTGGCAGCCGCTTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTGCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtc                                                                                                                            <  2:255081‑M1/250‑8 (MQ=255)                                                                                                                         cGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctc                                                                                                                         >  2:169821‑M1/1‑241 (MQ=255)                                                                                                                           gCTTTAAGTGCCTGCGCGGGATGACCCTTAACGATGCGAAAAGCACCATTGGCAGCCGCGTTGTCCGCCATCATAGCCTCGGTGAACGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGTATATCTGGGCAGAAGAGAGCGCCTGGCTGAAATCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacctcctctg                                                                                                                       <  2:107933‑M1/251‑13 (MQ=255)                                                                                                                              ttAAGTTTCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggt                                                                                                                     <  2:301955‑M1/250‑15 (MQ=255)                                                                                                                              ttAAGTATCTGCGCGGGATGCAGCGTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCGGATCCTCGAAACCAGCAACCCGGATATCTGGGCAGAAGAGAGCGCCTGGCTGAAAGCGCGACAAACTGAGAAAGCGGTAGCCTGCAGAgcacgtcctctggt                                                                                                                     <  2:92915‑M1/250‑15 (MQ=255)                                                                                                                                    aTCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaat                                                                                                              >  2:44142‑M1/1‑230 (MQ=255)                                                                                                                                     tCTGCGCGGGATGCACCTTAACGGTGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaat                                                                                                              <  2:274261‑M1/250‑22 (MQ=255)                                                                                                                                            gggactcaccttaACGATGCGAAAAGCACCTTTGGCAGCCGAGTTGACCGCCATCATAGCCTCGGAGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGACATTCCGCTGATCCTCGAAACCATCAACACGGATATCTGGGAAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAACGGTAGCCTGCAGAgcacgtcccctggtaaaaaattaacaacc                                                                                                      >  2:410009‑M1/7‑222 (MQ=255)                                                                                                                                            gggATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaacc                                                                                                      <  1:347189‑M1/251‑30 (MQ=255)                                                                                                                                                    ccTTAACGTTGCGCAAAGCACCTTTGGCATGCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGCCCGTGTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacggcctctggtaaaaaattaccaaccctgtcaat                                                                                              <  2:7298‑M1/251‑38 (MQ=255)                                                                                                                                                    ccTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCATGAAGCgcacgtcctctggtaaaaaattaccaaccctgtcaat                                                                                              >  1:403415‑M1/1‑214 (MQ=255)                                                                                                                                                       tAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATAATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCCAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgaacgtcctctggtaaaaaattaccaaccctgtcaatcc                                                                                            >  2:333007‑M1/1‑211 (MQ=255)                                                                                                                                                          cGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccaca                                                                                         >  1:442822‑M1/1‑208 (MQ=255)                                                                                                                                                                  aaaGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatat                                                                                  >  1:114808‑M1/1‑200 (MQ=255)                                                                                                                                                                     gCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagat                                                                              >  1:408718‑M1/1‑197 (MQ=255)                                                                                                                                                                        ccTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactg                                                                          <  1:370238‑M1/251‑58 (MQ=255)                                                                                                                                                                             ggCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaag                                                                     <  1:7916‑M1/251‑63 (MQ=255)                                                                                                                                                                                   cgcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtt                                                               >  2:27405‑M1/1‑183 (MQ=255)                                                                                                                                                                                    gcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggttt                                                              >  1:347773‑M1/1‑182 (MQ=255)                                                                                                                                                                                    ccttggaccgcCAACATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATACCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggttt                                                              <  2:240736‑M1/246‑70 (MQ=255)                                                                                                                                                                                        tGACCGCAATCATAGCATCGGAGAAGGCAATATAGGTCAAGATGCGTTCCGCTGGATAATGCAAGACGACCGTTTAGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcccgtcctctggtaaaaaattaccaaccctgtcaatccccaagaatatagacacagcaacgacggtttaaac                                                          >  2:143046‑M1/1‑178 (MQ=255)                                                                                                                                                                                          aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacat                                                        <  1:211259‑M1/251‑76 (MQ=255)                                                                                                                                                                                           ccGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGTCCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGAGATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCTGTAGCCTGAAGAgcacgtcctctggtaaaaaatttccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatg                                                       <  1:329598‑M1/251‑77 (MQ=255)                                                                                                                                                                                                atcatAGCCTCGGTGAAGGGAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttc                                                  <  2:372918‑M1/251‑82 (MQ=255)                                                                                                                                                                                                atcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttc                                                  >  1:401197‑M1/1‑170 (MQ=255)                                                                                                                                                                                                     aGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCGCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatat                                              <  1:292732‑M1/250‑86 (MQ=255)                                                                                                                                                                                                      gCCTCGGTGAAGGCAATATCGGTCATGATGCGGTCCGCTGGCTCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatttga                                            <  2:56010‑M1/251‑88 (MQ=255)                                                                                                                                                                                                                  gCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctc                                <  2:408718‑M1/251‑100 (MQ=255)                                                                                                                                                                                                                   cAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctc                                >  2:282883‑M1/1‑151 (MQ=255)                                                                                                                                                                                                                    aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAACAGCGGTAGCCTGAAGAgcacgtcctctggtacaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagccggtttaaacatgtgttcatatttaaacatgtgctcat                              >  1:246285‑M1/1‑150 (MQ=255)                                                                                                                                                                                                                         cGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagta                          <  1:291401‑M1/250‑106 (MQ=255)                                                                                                                                                                                                                                   gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgtttt                <  1:403665‑M1/250‑116 (MQ=255)                                                                                                                                                                                                                                         cGCTGGATCATGCAAGACGAACGTTTCGACGGCCTTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGATATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaagacggtttaaacatgtgttcatatttaaacatgtgcgcatagtatgcatgttttacgtaaa         <  2:256769‑M1/251‑123 (MQ=255)                                                                                                                                                                                                                                                 cATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCAGCAACCCGGATATCTGGGCAGAAGAGATCGCCGGGCTGAAAGCGCAACAAACTGAAAAATCGGTAGCCTGAATAgcacgtcctctggtaaaaaattaccaaccctgtcaatccacaagaatatagatactgcaaaggcggtttaaacatgtgttcatatttaaacatgtgctcatagtatgcatgttttacggaaagtaaatt  <  1:317349‑M1/250‑130 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCA  >  NC_000913/2251340‑2251829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑