Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,954	A→G	intergenic (+23/+78)	yoeF → / ← tmaR	putative uncharacterized protein YoeF/PTS system regulator TmaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,954	0	A	G	100.0%	146.3 / NA	39	intergenic (+23/+78)	yoeF/tmaR	putative uncharacterized protein YoeF/PTS system regulator TmaR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

CAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGATTTAGCCAGCATGGAACAAATATCTTTGGTGAAAAGTTGGTTACGGGAAGCTGGAGCGCACAACGTGTTAGTAACCAGTGCAGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGAAGGCTGTTGTTAATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTCATCC  >  NC_000913/2078716‑2079198                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     cgtgttaacgtccgacaaattgggtcattgacagggtgttctgtggattgactcatgtcattcagataatgaattaatgctactgcctgaacaaccagcaaacgggcattgtgtcctgaaatccaggctattgattcaacgtcagcgacagatctgctctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATGGGTTATCGGATGAGGATGATGTCGGt                                                                                                                                                                                                                                           <  2:424299‑M2/36‑1 (MQ=255)    gttaacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaagccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaattgtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGAt                                                                                                                                                                                                                                        <  2:289810‑M2/39‑1 (MQ=255)      taacgtcagacaaaatgcgtcattgacagcggtttctgtgtattgacacatttcattcagctaatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgcctatAATTTAGATTGGTTCTCGGATGAGGCTGATGTCGGTGATac                                                                                                                                                                                                                                      >  2:97258‑M2/210‑250 (MQ=255)       aacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATaca                                                                                                                                                                                                                                     <  1:263244‑M2/42‑1 (MQ=255)                 aaaattcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgcctgaacaaccagcaaacgggcatggtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaactggtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCGCGTATGAGGATGATGACGGTGATACACAGATAAGAAt                                                                                                                                                                                                                          <  2:333770‑M2/53‑1 (MQ=255)                              gacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaatg                                                                                                                                                                                                              >  1:7787‑M2/186‑250 (MQ=255)                                   cggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGtt                                                                                                                                                                                                        >  1:29246‑M2/181‑251 (MQ=255)                                   cggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGtt                                                                                                                                                                                                        <  1:251143‑M2/71‑1 (MQ=255)                                       ttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCg                                                                                                                                                                                                    >  2:253379‑M2/177‑251 (MQ=255)                                             ggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAAc                                                                                                                                                                                              <  1:126241‑M2/81‑1 (MQ=255)                                               attgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAAccc                                                                                                                                                                                            <  1:92596‑M2/83‑1 (MQ=255)                                                                 gataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACggg                                                                                                                                                                           >  2:194046‑M2/151‑250 (MQ=255)                                                                 gataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattagccggaatggtggctactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGATGGTGGCCGGGCAACCCTCATTGAATAAAACGGGa                                                                                                                                                                          <  1:133392‑M2/101‑1 (MQ=255)                                                                     atgacttaatgcaactaccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgacagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATAGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCATTGAGGGTGGCCGGGCAACACTCATTGAATAAAACGGGAAtt                                                                                                                                                                       >  2:224048‑M2/147‑250 (MQ=255)                                                                                        ggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTc                                                                                                                                                   <  1:127269‑M2/124‑1 (MQ=255)                                                                                            caaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCAttt                                                                                                                                               <  2:62347‑M2/128‑1 (MQ=255)                                                                                                        gggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCtt                                                                                                                                   <  1:194046‑M2/140‑1 (MQ=255)                                                                                                           cattgtgttctgaaatccaggctattgattcaacgtcagcgaccgatctgcgctgtaaaagaacttgcaaaacaacgtcatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGATTCGCCGTTTTTCATTTCGCCCATAGCTTTc                                                                                                                                 >  1:120418‑M2/109‑250 (MQ=255)                                                                                                                      gaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggtatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGGGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGGTTCGCCGTTTTTTATTTCTCCCATAGCTTTCAGCTTTttggtg                                                                                                                     >  2:248463‑M2/98‑249 (MQ=255)                                                                                                                                  attgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCgcggc                                                                                                           <  1:261166‑M2/164‑1 (MQ=255)                                                                                                                                         caacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTgg                                                                                                  <  2:66622‑M2/173‑1 (MQ=255)                                                                                                                                                 gcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCg                                                                                          >  1:287807‑M2/71‑251 (MQ=255)                                                                                                                                                    accgatcggcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggactggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACTCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGGTTTTCATTTCGCCCATAGCTTTAAGCTTTTGGGAGATATCGCGGCGTTCTTTGGAGAGCTCGgcc                                                                                       >  2:256010‑M2/68‑250 (MQ=255)                                                                                                                                                            gcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGTTTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTtga                                                                                <  1:383169‑M2/191‑1 (MQ=255)                                                                                                                                                                   aaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatGATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTGCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTaa                                                                        <  1:40381‑M2/199‑1 (MQ=255)                                                                                                                                                                                   caacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCATCCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGgag                                                                                                <  2:283260‑M2/175‑1 (MQ=255)                                                                                                                                                                                   caacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGgag                                                                                                >  1:283260‑M2/37‑211 (MQ=255)                                                                                                                                                                                   caacgtaatagaattatccggaatggtggcgactatAATTGCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCtt                                                        <  1:224048‑M2/215‑1 (MQ=255)                                                                                                                                                                                          atagaattatccggaatggtggcgactatAATTTCGAGTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTGCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTc                                                 <  2:377794‑M2/222‑1 (MQ=255)                                                                                                                                                                                             gaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTAGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCAcc                                              >  2:394595‑M2/27‑251 (MQ=255)                                                                                                                                                                                                 tatcaggaatggtggcgactatAATTTCGATTGGTTCTCGTATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGTGCTATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGACAACGCGATCTTCATAGTCACCTTTc                                          >  2:232254‑M2/23‑251 (MQ=255)                                                                                                                                                                                                     cggaatggaggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCACTGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGc                                      >  2:202753‑M2/19‑251 (MQ=255)                                                                                                                                                                                                         atggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAACACGGGAATTACTTCTCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGGTGTAATCGTCAACGCGATCTTCATCTTCCCCTTTCCTGCTGGc                                  >  2:21741‑M2/15‑251 (MQ=255)                                                                                                                                                                                                              ggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCgatg                              <  1:340018‑M2/241‑1 (MQ=255)                                                                                                                                                                                                               gcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCgatgat                            <  1:104193‑M2/243‑1 (MQ=255)                                                                                                                                                                                                                   ctatAATTTCGATTGGGTCCCGGATGAGGATGATGACGGTGATACACAGATAAGAATTAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAAAGGGAATTACTTCGCTTCGCCGGTTTTCATTTCGCCCATAAGTTTCAACTTTTTGGTGAGCTCGCTGCGTTTTTTGGCGAGCTCGGCATTTTTGATTATGTAATCTTCAACGCCAGTTTAATAGTCACCTTTTCTGCTGGCGATGATGccc                        >  2:286925/4‑251 (MQ=255)                                                                                                                                                                                                                       aaTTTCGATTGGTTCTCGGATGAGGAGGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGa                    <  1:234705/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggatctcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAAc          >  1:185911/4‑251 (MQ=255)                                                                                                                                                                                                                                         gATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTCATcc  >  2:265823/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     CAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGATTTAGCCAGCATGGAACAAATATCTTTGGTGAAAAGTTGGTTACGGGAAGCTGGAGCGCACAACGTGTTAGTAACCAGTGCAGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGAAGGCTGTTGTTAATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTCATCC  >  NC_000913/2078716‑2079198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑