Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	112.4 / NA	33	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/18);  total (15/18)

TATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652086‑1652574                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tATGGTGTGTTTCGGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCag                                                                                                                                                                                                                                                  <  2:272797/250‑1 (MQ=255)    ggTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGCACCAATCCGGTCATTCCTCTTATTAAGCCAGCAATCGACGCCCTCCGGGGTCCGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                                                >  1:407039/1‑249 (MQ=255)       gtgtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTAt                                                                                                                                                                                                                                           <  1:403618/251‑1 (MQ=255)                                    ggTGAGCTGTGTGCACGTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCtt                                                                                                                                                                                                             <  2:434335/251‑1 (MQ=255)                                       gAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGt                                                                                                                                                                                                          <  2:417787/251‑1 (MQ=255)                                         gCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGttt                                                                                                                                                                                                        <  1:62930/251‑1 (MQ=255)                                                gCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAAc                                                                                                                                                                                                  <  1:195518/250‑1 (MQ=255)                                                       gATGGGGGGACATAGATCTGAAACATGGAACAATGATGATCAGGAGAAATGTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGACCAAAAGGGTCATTCATCTTATTAAACAAGCAAATGACGCCATCCCGAGTCAGATGGCATTAACGAGACTGAGCAAAAAGCATAAAATTGGTGGTTACCCCAGAGAGTAGTGCAAAACAAAAAAACAACAAATGAACCTT‑GTTTTTCaaaccgcaatg                                                                                                                                                                                          >  2:314454/1‑242 (MQ=255)                                                                            aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAATAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt                                                                                                                                                                     <  1:188570/251‑1 (MQ=255)                                                                            aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGAACCAAAAACACAGGCGGGAACCAACCGGGGAATTCATCTTATTAAAACAGCAATTGACGATCTCCGGAGTCAAAAGACATTAACGGGACTGAGCAAAGAGCATCTAATTTGTGTTCACCTCAGAGCGTATGGCAGAACAAAAAAACAA‑AAATACACCTTTTTTTTTCATACTGAAGTGTccacgggaggaaaaaaatat                                                                                                                                                                     >  2:359612/1‑232 (MQ=255)                                                                               aTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTTAGATGACATTAACGAGACTGAGCCAAAAGCATATCATTGATGTTCACCTCAGAGAGGATTGCAGAAAAGAAAAAAAA‑AAATGCACCTTTGTTTTTTAACCTGAAGGGGCAGCGAGAGTAAAAAATTATggg                                                                                                                                                                  >  1:283082/1‑250 (MQ=255)                                                                                                aGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAA‑AAATGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGGGACCATTTTAACGTTg                                                                                                                                                  >  1:160938/1‑250 (MQ=255)                                                                                                        tttAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGTAACCCATCGGGTCCTTCCTCTTATTAAGACAGCAAACGACGCTCTCCGGCGTCAGAAGACATTAAAGAGACTGAGCAAAAAGCATATCATTTATGTTCACCTCAGAGAGTTTGGCAGAACAAAAAAACAA‑AAATTCACCTTTTTTTTTTAACCTGAAGTGTCTACGAGGGGAAAAAATTATGGGGACCATTTTACCGTTGACTccatt                                                                                                                                          >  2:398016/1‑246 (MQ=255)                                                                                                                  gACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAACCAGCAATCGACGCTCTCCGGGGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGAtgtg                                                                                                                               >  1:111559/1‑251 (MQ=255)                                                                                                                          cACGGTACCAAAAACTCAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAGGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCa                                                                                                                        <  2:23684/250‑1 (MQ=255)                                                                                                                                           aGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg                                                                                                       >  2:390788/1‑250 (MQ=255)                                                                                                                                            gtcGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGACAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTTGGGTGACCATTTTACAGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACCTGCCGGAc                                                                                                     >  2:25874/3‑251 (MQ=255)                                                                                                                                                     cAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATc                                                                                            <  1:125289/251‑1 (MQ=255)                                                                                                                                                       aTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGa                                                                                          <  1:277718/251‑1 (MQ=255)                                                                                                                                                                 tcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc                                                                                >  2:277567/1‑251 (MQ=255)                                                                                                                                                                       tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGt                                                                             <  1:148463/248‑1 (MQ=255)                                                                                                                                                                       tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATGAACGAGACTGAGCAAAGAGCATATCATTGATGTTAACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCatataag                                                                              <  2:235547/247‑4 (MQ=255)                                                                                                                                                                       tagtaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGACTATTGCAGAACAGAAAAACAA‑AAATGCACCTTTTTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACTAAGCGATAAAACTTGCCGGACTCCGCCATCGAAAATCATATCAGt                                                                             <  2:190748/245‑1 (MQ=255)                                                                                                                                                                                          gCTCTCCGGAGTCCGATGACATTAACGAGACTGCGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgctg                                                       >  2:109729/1‑251 (MQ=255)                                                                                                                                                                                               ccGGAGTCAGATGACATGAACGAGACTAAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTTTGTCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAGCCGGTATTGTCAGCGAGAGTAATAAATTATGGTGACCATGTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAGCATAGCAGTCGTGACATACTTATGCCTGCTGGTc                                                    <  2:182960/249‑1 (MQ=255)                                                                                                                                                                                                cGGAGTCAGATGACATTAACGAGGCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTTACCATTTTACCGTTGACTCAATAAGGCAGATGTGTGACGCAGCGATAAATCGTTCCGGACTCCGCCATCGAAAATCATAGCAGTCGAGACATACTTATGCCTGCTGGTCGCt                                                 <  2:283082/251‑1 (MQ=255)                                                                                                                                                                                                           gACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACGTATGCCTGCTGGTCGCTGACAGCTGGt                                       <  1:109729/250‑1 (MQ=255)                                                                                                                                                                                                              aTTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGTACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTa                                   <  2:111559/251‑1 (MQ=255)                                                                                                                                                                                                                          gAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAttt                        >  2:43500/1‑250 (MQ=255)                                                                                                                                                                                                                              aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc                   >  1:70770/1‑251 (MQ=255)                                                                                                                                                                                                                                         cATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGAAGATGCTAACCCGGCATTTATAGCAAACCAGATg         <  1:43500/250‑1 (MQ=255)                                                                                                                                                                                                                                           ttGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGc      >  1:289514/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGAGTCAGAGAGAGTAAACAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATg  >  2:14821/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAA‑AAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652086‑1652574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑