Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	254,249	A→T	intergenic (+1088/+10)	yafP → / ← pepD	putative N‑acetyltransferase YafP/peptidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	254,249	0	A	T	95.7%	158.6 / NA	47	intergenic (+1088/+10)	yafP/pepD	putative N‑acetyltransferase YafP/peptidase D
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (18/26);  minor base G (0/2);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGC  >  NC_000913/254005‑254493                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aTTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcatatcaaa                                                                                                                                                                                                                                                 <  1:442605/251‑1 (MQ=255)    aGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAaata                                                                                                                                                                                                                                               <  2:76141/250‑1 (MQ=255)     gCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAaataat                                                                                                                                                                                                                                             <  1:8974/251‑1 (MQ=255)                       agCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGGCGGAAtttct                                                                                                                                                                                                                           <  2:350858/251‑1 (MQ=255)                          gTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAAGCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAAtttctttc                                                                                                                                                                                                                        <  2:44328/251‑1 (MQ=255)                                     gCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt                                                                                                                                                                                                             >  1:354545/1‑251 (MQ=255)                                        aaCAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtc                                                                                                                                                                                                            <  1:331464/249‑1 (MQ=255)                                                  ttgaaCGATTGCTGATTGCCTGGAAGCTGTAGCAACAGCATCAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGGGGAAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAAATGCAGGAAGGCGATTAAAGGCCATCAGGCAGAATATCAACTAATTACTTCTCCGGAATTTATTTCAGCAGTtcagtatccagtggaccg                                                                                                                                                                                                >  2:262132/5‑238 (MQ=255)                                                            gCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCt                                                                                                                                                                                      <  1:265434/250‑1 (MQ=255)                                                            gCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTa                                                                                                                                                                                     <  1:442583/251‑1 (MQ=255)                                                                   gCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGCCCTACGCttt                                                                                                                                                                               <  1:311112/250‑1 (MQ=255)                                                                         aaGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGAtgtg                                                                                                                                                                        >  1:68538/1‑251 (MQ=255)                                                                                agcaacagcaacAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGATCTTGc                                                                                                                                                                 <  2:340777/251‑1 (MQ=255)                                                                                      agcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCATTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATcc                                                                                                                                                           <  1:343158/251‑1 (MQ=255)                                                                                      agcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATcc                                                                                                                                                           <  1:19490/251‑1 (MQ=255)                                                                                               acaaTAGTGCGGAGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCAACGGACATTTACAGGGATGGCTTTTATCGAAGGATCATGAAGGATGAAACTTCAGGAAGGCGATTAAACGCCATCCGGCAGAATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGAAGTGT‑CCAGTAATGACATACGCCTTAGATGTGAACTTGCTCATCAGGAGGgtgt                                                                                                                                                  >  2:169795/3‑251 (MQ=255)                                                                                                  aTAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTgg                                                                                                                                                >  2:406969/1‑250 (MQ=255)                                                                                                  aTAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGa                                                                                                                                               <  1:121846/251‑1 (MQ=255)                                                                                                           cgcTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATa                                                                                                                                      <  1:113529/251‑1 (MQ=255)                                                                                                                           gcgAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGaa                                                                                                                       >  1:222625/1‑250 (MQ=255)                                                                                                                           gcgAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGAGAGTTGGCCCGATAGaaa                                                                                                                      <  1:301014/251‑1 (MQ=255)                                                                                                                             gAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAAcc                                                                                                                    >  2:273623/1‑251 (MQ=255)                                                                                                                                      aTCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAATCGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAtt                                                                                                           >  1:325814/1‑251 (MQ=255)                                                                                                                                      aTCACCAGATTGAACGTGGCAACCCGCGACGGACATGTACAGGGATGGCTTTTCTCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAGATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGGGCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAATCCATGTTCAtt                                                                                                           <  2:230414/251‑1 (MQ=255)                                                                                                                                        taCCAGATTGAACGGTGCAGCCCGCGACGGACATTTACAAGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGTCATCCGGCAGCATATCAAATAATTACTGCGCCGGAATTTCTTTCAGCAGTTCAGTCAGTAGTGT‑CCAGTAATGGTCTGCGATGTCGATGTGAACTTGATCATCCGGAGAGTGTTGACCGGTGATAGTTGGTCCGATAGATACCGTGTCCAttt                                                                                                          <  2:343588/249‑1 (MQ=255)                                                                                                                                                     gTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtttt                                                                                            >  1:382168/1‑251 (MQ=255)                                                                                                                                                       ggCAACCCGCGACGGACATTTACAGGGATGGATTTTATCGAAGGATAATGAAGGATGAAAATGCCTGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCTTTTCCGGATACGGtttttt                                                                                          <  2:194139/251‑1 (MQ=255)                                                                                                                                                         cAACCCGCTACGGACATTTACATGGATGGCTTTTATCGAAGGATAATTAAGGATGAAACTGCCGGAAGGCGATTAAACTCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGATAGTGTGGACCGGTGATAGTTTGCCCGATAGAAACCATGTCCATTTCCGTATACGGTTTTTTGa                                                                                        <  2:234414/251‑1 (MQ=255)                                                                                                                                                           aCCCGCGACGGACATTTACAGGGATGGCCTTTATCGATGGATAATGAAGGATGAAACTGCCAGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAACCCATGTCCATTTCCGGATACGGTTTTTTGaa                                                                                       >  2:198529/1‑250 (MQ=255)                                                                                                                                                              cgcgACGGACATTTACAGGGGTGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAGACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAAc                                                                                      >  2:444376/1‑248 (MQ=255)                                                                                                                                                                     gACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCTCAtt                                                                            <  1:360693/251‑1 (MQ=255)                                                                                                                                                                      aCATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGTCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTc                                                                           <  2:204488/251‑1 (MQ=255)                                                                                                                                                                          ttACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAg                                                                        <  1:266870/250‑1 (MQ=255)                                                                                                                                                                                ggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCcgcg                                                                 <  2:222625/251‑1 (MQ=255)                                                                                                                                                                                 gATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGt                                                                <  1:406969/251‑1 (MQ=255)                                                                                                                                                                                               aaGGATAATGAAGGAGGAAACTGCCGGAAGGCGATGAAACGCCATTCGGAAGAAGATCAAATAATTACGTCGCCGGAATTTCTGTCAGCAGTTCAGTCAGCAGTGT‑CCAGTTATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTGGGCCCGATATAAACCATGTGCATTGCCGGATATGGTGGTTTGAATAGGGGACACTCCAGGCTCGCGTGGATAAGCAGGATg                                                  <  2:150732/251‑1 (MQ=255)                                                                                                                                                                                                   aTAATGAAGGGTGATACTGCAGGAAGGAGTTGAAACGCCATCCGGCAGCATATCAAATAAGTACTGCGCCGGTATGTCTTTCAGCAGTTCAGTCAGCAG‑GTGCCAGGAATGACCTACGCTTTCGATGGGAACTTGCTCATCCGGAGAGTGTGGACCGGTGAAAGTTGTCCCGATATAACGTATGTCCAGCTCCGGATACGGTTTTTTAAACTGACAACATTCCTGGCGGGCGTAGATAATCTggtggttcg                                              <  2:338019/251‑8 (MQ=255)                                                                                                                                                                                                     aaTGAAGAATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGc                                            <  1:312934/251‑1 (MQ=255)                                                                                                                                                                                                         aaGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCCAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGCTAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCt                                        >  1:385866/1‑251 (MQ=255)                                                                                                                                                                                                            gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGAGCGGACCGGCGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGCTAATCTGGATGTTCGGCgctttgt                                     >  2:123773/1‑245 (MQ=255)                                                                                                                                                                                                                   cTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAACTAATTACTTCGCCGGACTTTCTTTCAGCAGTTCAGTCAGCAGTGT‑ACAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTACAGGCCCGCGTGGGTAATCTGGAAGTTCGTCGTCTTGTTGAAc                                >  1:321414/1‑249 (MQ=255)                                                                                                                                                                                                                     gCCGGAAGGCGATTAAGCGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGGGGAGAATCTTTATGTTCGGCGTCTTGTTGAACAg                              <  2:325814/249‑1 (MQ=255)                                                                                                                                                                                                                      tcgtaaGGCGATTAAACGCCATCCTGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCCGTCAGCAGTGT‑CCAGCAATGACCTACGCTTTCGATGTGAACTTGCTCCTCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGCTAGAAACCATGTACATTTACGGATACGGTTTTTAGAACAGACAACATTCCAGGCCAGCGTGGATAATCTGGATGTTCGGCGGCTTGTTGAAAAGgcg                           >  2:444982/5‑251 (MQ=255)                                                                                                                                                                                                                       cGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGCTAATCTGGATGTTCGGCTTCTTGTTGAACAGgcgc                          >  2:112622/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaCGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTc               >  1:129198/1‑250 (MQ=255)                                                                                                                                                                                                                                            ccGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGGCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATGCCAGGCCCGCGTGTATAAGCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGa     <  2:142149/251‑1 (MQ=255)                                                                                                                                                                                                                                               gcagcaTATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGc  >  2:227631/1‑251 (MQ=255)                                                                                                                                                                                                                                                cagcaTATCAAATAATTACTTCGCCGGAATTTCTTTAAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATCATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGc  >  2:303307/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGT‑CCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGC  >  NC_000913/254005‑254493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑