Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,463	A→T	*247K (TAA→AAA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,463	0	A	T	100.0%	181.7 / NA	45	*247K (TAA→AAA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/24);  total (21/24)

ACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCT  >  NC_000913/155224‑155692                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      acacTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt                                                                                                                                                                                                                            >  2:33277/1‑251 (MQ=255) acacTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt                                                                                                                                                                                                                            >  2:304943/1‑251 (MQ=255)  cacTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCTCAAAACGTGGCGATACTGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt                                                                                                                                                                                                                           <  1:339183/251‑1 (MQ=255)      gACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc                                                                                                                                                                                                                         <  1:397875/249‑1 (MQ=255)         gtcgtAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa                                                                                                                                                                                                                    <  1:412605/251‑1 (MQ=255)           cgtAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTGGCCTGATTTCAGCCTGGCATTACCTTCAAt                                                                                                                                                                                                                  <  1:103658/251‑1 (MQ=255)                        tataGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaa                                                                                                                                                                                                       >  1:263722/1‑249 (MQ=255)                                          aCTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa                                                                                                                                                                                    >  1:42138/1‑250 (MQ=255)                                                  ggCTGCGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTAAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACttt                                                                                                                                                                           >  1:278400/1‑251 (MQ=255)                                                  ggCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACttt                                                                                                                                                                           <  1:39964/251‑1 (MQ=255)                                                      gagaTCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATAGCCTTGCCTGATTTCAGCCTGGCATTTCCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGc                                                                                                                                                                        <  2:358956/250‑1 (MQ=255)                                                              tatTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGc                                                                                                                                                               >  1:282662/1‑251 (MQ=255)                                                                                  aTCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTg                                                                                                                                            >  2:173554/1‑250 (MQ=255)                                                                                  aTCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTg                                                                                                                                            >  2:412264/1‑250 (MQ=255)                                                                                  aTCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGa                                                                                                                                           <  1:122347/251‑1 (MQ=255)                                                                                       gAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTc                                                                                                                                      >  1:83593/1‑251 (MQ=255)                                                                                        aaGGTATGGTCATATTCAACGCGGTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTAATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTc                                                                                                                                      >  2:283489/1‑250 (MQ=255)                                                                                                    tatTCAACGAGTTCAGCTCTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATGCTATAGTCACGCTATGCTTCCTGTGTAATTATATCCGTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc                                                                                                                                                                                                               <  2:377145/165‑1 (MQ=255)                                                                                                    tatTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc                                                                                                                                                                                                               >  1:377145/1‑165 (MQ=255)                                                                                                          aCGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTTCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCAAGACCTCATCACTAAATg                                                                                                                   <  2:356337/251‑1 (MQ=255)                                                                                                                 cAGCACTGAAAGCAGTATTGCAATACAGCAGCTCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCTCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAAGGGTGCAAt                                                                                                            <  2:350104/251‑1 (MQ=255)                                                                                                                             cAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATc                                                                                                <  1:57783/251‑1 (MQ=255)                                                                                                                               gTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCa                                                                                               >  2:341032/1‑250 (MQ=255)                                                                                                                                      aaTACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATa                                                                                       <  1:180894/251‑1 (MQ=255)                                                                                                                                         aCAGCAGCGCGCAAAACGTGGCGATACGGGTCCGATGATGATAATTTTTCGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTACTGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCg                                                                                    >  2:340389/1‑251 (MQ=255)                                                                                                                                           agcagcGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGtt                                                                                  >  2:284777/1‑251 (MQ=255)                                                                                                                                               gcgcgcAAAACGTGGCGAGACGGGTCCGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTTACTGATTTCAGCCTGTCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTTAATGGTGCAAACAGTTTCTCATCAATCGGATATCCTTTAcc                                                                              <  2:111290/251‑1 (MQ=255)                                                                                                                                                 gcgcAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGc                                                                            <  1:74556/251‑1 (MQ=255)                                                                                                                                                     aaaaCGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGTACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGc                                                                        <  2:72520/251‑1 (MQ=255)                                                                                                                                                      aaaCGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTTAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCt                                                                       <  1:304943/251‑1 (MQ=255)                                                                                                                                                      aaaCGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCt                                                                       <  1:33277/251‑1 (MQ=255)                                                                                                                                                              cGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATc                                                               >  2:229864/1‑251 (MQ=255)                                                                                                                                                                  aCGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATcacca                                                           <  2:42138/251‑1 (MQ=255)                                                                                                                                                                      gTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGc                                                       >  2:435164/1‑251 (MQ=255)                                                                                                                                                                                    aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTATCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACgt                                           >  1:439646/1‑249 (MQ=255)                                                                                                                                                                                      ttttATTATAATCTATAGTCACGCGATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAACTGGTGCAATCATTTTCACATCAATCGGCTAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACgaagt                                        >  2:234074/1‑250 (MQ=255)                                                                                                                                                                                          aGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAggg                                   >  1:310342/1‑251 (MQ=255)                                                                                                                                                                                               atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTg                               <  1:284777/250‑1 (MQ=255)                                                                                                                                                                                                ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTgg                              >  1:370902/1‑250 (MQ=255)                                                                                                                                                                                                      aGTCACGATATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCTTTACCTTCAATTGCGACACCACAATCATAAATGGCGTAAAAATGCACTTATGCAGAATACGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACAACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTc                       >  2:355483/1‑251 (MQ=255)                                                                                                                                                                                                           cGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCg                  <  2:83593/251‑1 (MQ=255)                                                                                                                                                                                                               aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAAt              <  1:412264/251‑1 (MQ=255)                                                                                                                                                                                                                     ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGc         <  1:139731/250‑1 (MQ=255)                                                                                                                                                                                                                        gcagaattagttCCTTGCCTTATGTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCTCGTTTGCATAAGTCGATTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCAGTTTCACTTCAAGCGGATAGCGTTTACCGCTAGCTTCTAAATCTCCCCTGCTAAAAGAGACGTAGTAAGGGTTTGGCTTGGTCACTCGTAATTACGcatt      <  2:174650/239‑4 (MQ=255)                                                                                                                                                                                                                           gAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCt  <  1:199444/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      ACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCT  >  NC_000913/155224‑155692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑