Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,626	0	G	A	24.5%	80.5 / 15.0	49	F180F (TTC→TTT)	ycfT	inner membrane protein YcfT
Reads supporting (aligned to +/- strand):  ref base G (28/9);  new base A (0/12);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 3.19e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCAC  >  NC_000913/1174376‑1174868                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      tACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑g                                                                                                                                                                                                                                                       >  1:372369/1‑251 (MQ=255) tACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑g                                                                                                                                                                                                                                                       >  1:350796/1‑251 (MQ=255)    cGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑Gaa                                                                                                                                                                                                                                                     >  1:391090/1‑250 (MQ=255)     gAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAAt                                                                                                                                                                                                                                                   <  2:348654/251‑1 (MQ=255)                    ttGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCAACGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCTACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑CGCGCCAAGG‑CTCTAATAAGGCAAATTGAGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACGGCCACACTc                                                                                                                                                                                                                                     >  2:370154/1‑250 (MQ=255)                               gcttcagaatacccaaaaccGATCCCAGCGACAACGGCAGCGAGAAAATAAAAAACCAGCCCCCGCCCACCAGAACGGTAAGAAAAGAAGCCATCAGCAGAGGGGGCCGCACCG‑GCACCTTTTTAACAAGGGTATTTTTTGTTG‑CGCCAAAC‑CA‑TGCGCCAGGG‑TTTAAATAGGGAAAATTGCGGATCACACG‑TTTCTTCCCCCACCACGG‑GGGGGAA‑CCAAAATTAACCGCCACACTCAGCAGTacaaac                                                                                                                                                                                                                         <  1:154196/232‑1 (MQ=255)                                   cATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAACAAACAACCAGGCACCGACCGCCAGAACGGAAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑AGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GACATTAACCGCCACACTCTGCAGTACAAACAA‑gg                                                                                                                                                                                                                    >  1:103562/1‑251 (MQ=255)                                                aTCGATACCAGCGAAAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATGGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAAGTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCTGCAGTACAAACAA‑GGCGAATAGCGGc                                                                                                                                                                                                         >  2:275607/1‑249 (MQ=255)                                                    aaaCCAGCGACAACAGAAGAGAGATATTAAACACCCGGGCCCCGACCGCCAGAACGGTAAGAAAAAACGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACCCAGGTCATTATTGTTG‑CCCAAAAC‑CA‑TGCCCCAAGG‑TTTTAATAAGGCAAATGGCGGGTCCCCCT‑GTTCATTCCCCCCCCCGGGGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGcc                                                                                                                                                                                                   <  1:346920/249‑1 (MQ=255)                                                       ccAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGCA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACCA‑GGCGAATAGTGGCAGCGcc                                                                                                                                                                                                   >  1:369765/1‑248 (MQ=255)                                                              caacaGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTa                                                                                                                                                                                          >  1:26958/1‑250 (MQ=255)                                                                 cagaagagagaTAGAAAACACCCAGGCCCCGCCCGCAAGAACGGTAAGAAAAGAACCCATCAGCAGATGGCGGCGAAACG‑GCCCCTCTTTAACACAGGTCATTATTGTGG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACCCT‑GTTCATCCCCCCCCCCGGCGGGGGAA‑C‑AAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTaaaaa                                                                                                                                                                                      <  1:55484/244‑1 (MQ=255)                                                                  agcagcGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAAt                                                                                                                                                                                     >  1:448926/1‑251 (MQ=255)                                                                    cagcGAGATAGTAAACAACCAGGCACCGAACGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAAttt                                                                                                                                                                                   >  1:310326/1‑251 (MQ=255)                                                                        gagaTAGGAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTAc                                                                                                                                                                                >  1:241590/1‑250 (MQ=255)                                                                        gaagaaaaaaaCACCCAGGCACCGACCGCCAGAACGGTAAAAAAAAACGCAAACAGCGGTGGGCGGCGCAACG‑GCCCCTCTTAAACACAGGCAATTATTGTTG‑CGC‑AAACACA‑TGCGCCAGGG‑CGAAAAAAAGGCAATTGGCGGATCACACT‑TTTCATCCCCCCCCACGGGGTGGAAA‑C‑AAAATAAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACa                                                                                                                                                                               <  2:160536/243‑1 (MQ=255)                                                                                    aacCAGGCACCGACAGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCCGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTACTAAGGCAACTTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACCCAGCAGTACAA‑CAACGGCGAATAGTGGCAGCGCCAGACGCCTCAAAACTTTACATACCACGAAAt                                                                                                                                                                    >  2:207128/1‑250 (MQ=255)                                                                                      ccAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑Ga                                                                                                                                                                                                                                                      >  1:148483/1‑167 (MQ=255)                                                                                      ccAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCGTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑Ga                                                                                                                                                                                                                                                      <  2:148483/167‑1 (MQ=255)                                                                                         ggCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGCAATAGACa                                                                                                                                                               >  1:266586/1‑250 (MQ=255)                                                                                           cACCGACCGCCAGAACGGTAAGCAAAGAAGCAATCCGCAGATGGCGGCGCAACG‑GCACCTCTTTAAAACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAtt                                                                                                                                                            >  1:336048/1‑251 (MQ=255)                                                                                                    cgaGAACGGAAAGCAAAGAACCTAGCAGAAGATGGCGGCGCACCG‑GCCCCTTTTTAACGATGGTCATTATTGTGG‑CGCCAAAC‑CA‑GGCGCAAGGG‑GTGTAATAAGGCAAATTGGGGATCACGCT‑TTTCATCCCCCACCCCGGGGGGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATAc                                                                                                                                                   <  2:449015/249‑1 (MQ=255)                                                                                                      gtaagggaaggaaaaGAACCCACCAGCGGAGGTCGGCGCAACG‑GCACCTATTAACCAGGGGTCTTTATTGTTG‑CGCAAAAC‑AAGTG‑GCAAAGT‑GTGTAATAAGGGAAATCGCGGA‑CCCACTTGTTCATCCCCCACCGCGGGGGGGAAA‑C‑AAAATTAGCCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAg                                                                                                                                                 <  1:394360/239‑1 (MQ=255)                                                                                                               aGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCACTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATCAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAAATTTACATACCACGAAATAGACAATTAACGCATACAGATACcaca                                                                                                                                         >  2:298652/1‑250 (MQ=255)                                                                                                                             ccatcagtgggcggcccagtgcacccTCTATAACACAGGACTTTTTTGTTC‑CCCCAAAC‑AA‑GGC‑CCAAGGGCTGTAAAAAGAAAAATTGGGGTGCCCACT‑GTTCCTTCCCCACCGCGCTGTGGGAA‑C‑AAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAGTAACGCATACAGATACCACAAGCTGGTGCTGGCg                                                                                                                           <  2:164208/227‑1 (MQ=255)                                                                                                                             cagcagAATGCGACGCAACG‑GCACCTCTTTAACACATGTCATTATTGTTG‑CGCCAAAC‑CC‑TGCGCCACGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACAGCCACACTCAGCAGTACAACCAC‑GGCGAATAGAGACAGCCCCAAACGGAAAAAAATTTAACCCACCACGCAATAGTAGATGAACGCATACAAATACCAAAAGcatgaccaggatg                                                                                                                          >  2:267163/1‑239 (MQ=255)                                                                                                                               gcagATGGGGGCGCA‑CGTGCACCTTTTAAACACAGGTAATGAT‑GTTGGCGCTAACA‑CA‑TGCGCCAGGG‑TTGTAAGAAGGCAAATGGGGGATCACACT‑CTCTATCCCCCACCACGGGGGGGAAA‑C‑GAAATAAGCCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTg                                                                                                                        <  2:84939/251‑1 (MQ=255)                                                                                                                                    tGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGatcat                                                                                                                   >  1:102895/1‑251 (MQ=255)                                                                                                                                     gggcggctcaccgcccccTCTTAAACACAGTTCATTTTGGGTG‑AGCCAAAC‑CA‑CGCGCCAGGG‑TTTAAAGAAGGCAAATGGCGGATCACCCT‑GTTCATCCCCCCCCACG‑CGGGGGAAAC‑GAAATAAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAAGTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGatcat                                                                                                                   <  2:102863/235‑1 (MQ=255)                                                                                                                                            cAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGgtcaactcgcgc                                                                                                            >  2:98844/1‑242 (MQ=255)                                                                                                                                              aCG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGatgctccccctccgg                                                                                                         >  2:112025/1‑238 (MQ=255)                                                                                                                                              aCG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑gg                                                                                                        >  2:291410/1‑251 (MQ=255)                                                                                                                                                          ttAACAGAGGTTATTTTTGTTG‑CCACAAAC‑CA‑GGCGCAAAGG‑CTGTAAAAAGGAAAATGGCGGACCACTCT‑GTTCTTTCCCCCCCCCGGCGGGGGAA‑G‑AAAATTAGCCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCgg                                                                                              <  2:179088/250‑1 (MQ=255)                                                                                                                                                           aaccacgggtttttatgGTTG‑CGCCAACC‑CG‑TGCGCAAAGT‑TTGTAAGAAGGAAAATGGCGGATCACCCT‑TTCCATCCCCCCCCCGGGCGTGGGAA‑A‑AAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTg                                                                                            <  1:334182/239‑1 (MQ=255)                                                                                                                                                                           tgttg‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAAAAAGGCAAATTGCGGATCACCCT‑GTTCATTCCCCCCCACGGCGTGGGAA‑C‑AAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCg                                                                             <  1:291410/250‑1 (MQ=255)                                                                                                                                                                            gttg‑CGCCAACC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGc                                                                           >  1:288363/1‑251 (MQ=255)                                                                                                                                                                                               cgcCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCtt                                                          >  1:288491/1‑251 (MQ=255)                                                                                                                                                                                               cgcCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGGGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCtt                                                          >  1:266472/1‑251 (MQ=255)                                                                                                                                                                                                  cAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAACCAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATATCACCAGCTGGTGCTGGCGGTGAACCTCCCGTGCA‑GGAACCCCCCCGGGGAATCGGCCTAAGTGGCA‑ATGGAGGCATTGCTTaa                                                        >  1:111391/1‑250 (MQ=255)                                                                                                                                                                                                    aGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGGGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATc                                                     >  1:259042/1‑251 (MQ=255)                                                                                                                                                                                                          gTAATAAGGCAAATTGCGGATCACCCT‑GTTCATTCCCCCCCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCt                                                <  1:66238/251‑1 (MQ=255)                                                                                                                                                                                                            aataaGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCCTCCCGTGCA‑GGAACACACCCGGGGAAACGGCCCAAACGGCAATTGG‑GGCATTTCCTTAATCGCGCTcc                                              >  2:183761/1‑250 (MQ=255)                                                                                                                                                                                                                 ggCAAATTGCGGATCCCACT‑GTTCATCCCCCCCCACGGGGTGGAAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTgc                                         <  1:67318/251‑1 (MQ=255)                                                                                                                                                                                                                      gttGCGAATCACACT‑TTGCAGCCCCCCCCCCGGGGTGGGCA‑C‑AAAATAACCCGCCACACGCAGCAGTACAAACAA‑GGCGAATAGTGGCGGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACATATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TGGGAGGCATTGCTGAAATCGCGCTCATGTgccagcc                                    <  2:338937/250‑1 (MQ=255)                                                                                                                                                                                                                        tGCGGATCCCTCT‑GTTCTTTCCCCCCCCCGGCGTGGGAA‑C‑AAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTgccagcca                                   <  1:275607/250‑1 (MQ=255)                                                                                                                                                                                                                         gCGGATCACACT‑GTTCATTCCCCACCACGGCGTGCGAA‑C‑GACATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCCGAGCCAGACGGCTAAAAATTTTACATAACACGAAATAGACAATTACAGCCTACAGATACCCCAAGCTGGTGCTGGCGGGGATCATCCCCGGCAAGGAA‑GAAACGGGGGAATCGGCACAACCGGCA‑TTGGAGGCATTGCTTAACTCGCGCCCAGGGgccacacaac                                 >  2:411338/1‑245 (MQ=255)                                                                                                                                                                                                                                        ccttcccccACCGCGGCGTGGAAA‑A‑GAAATAAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACt                    <  2:350796/245‑1 (MQ=255)                                                                                                                                                                                                                                        caatccccccccggggggTGGAAA‑C‑AAAATAAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACtt                   <  2:102895/234‑1 (MQ=255)                                                                                                                                                                                                                                               ccaccggggggTGGGAA‑C‑AAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgcca            <  1:411338/241‑1 (MQ=255)                                                                                                                                                                                                                                               ccaccaCGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAACGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgcc             >  1:167061/1‑250 (MQ=255)                                                                                                                                                                                                                                                         gTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGGATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGGTCAGCGCACTTAACGCCAGCCACTGcac  >  1:341884/1‑251 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      TACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACG‑GCACCTCTTTAACACAGGTCATTATTGTTG‑CGCCAAAC‑CA‑TGCGCCAAGG‑CTGTAATAAGGCAAATTGCGGATCACACT‑GTTCATTCCCCACCACGGCGTGGGAA‑C‑GAAATTAACCGCCACACTCAGCAGTACAAACAA‑GGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCA‑GGAACTCACCGGTGGAATCGGCATAAGCGGCA‑TTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCAC  >  NC_000913/1174376‑1174868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑