Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	30 [0]	[0] 30	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	30 (0.590)	26/490	0.7	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

GCGAGAATATAATAAGGGCCATTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT  >  NC_000913/3079152‑3079625                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                  gccgctttccaggtcgataaacagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaa                                                                                                                                                                                                                                   >  1:180191‑M2/249‑249 (MQ=255)               cgataaacagacgctgtcagaaattacactctccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttagtaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGa                                                                                                                                                                                                                   <  2:267578‑M2/17‑1 (MQ=255)                gataaacagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGaa                                                                                                                                                                                                                  <  1:99088‑M2/18‑1 (MQ=255)                    aacagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCg                                                                                                                                                                                                              >  1:448956‑M2/230‑251 (MQ=255)                      cagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGgt                                                                                                                                                                                                            >  1:54606‑M2/228‑251 (MQ=255)                                       tacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGTTGTTTTGAAAACAGTGa                                                                                                                                                                                            <  1:300397‑M2/40‑1 (MQ=255)                                       tacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGa                                                                                                                                                                                            <  2:54606‑M2/40‑1 (MQ=255)                                              accagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccagaaatagcagcaactttggttttcatttttacctttgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaagcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACATTGAATTATTaa                                                                                                                                                                                    <  1:171308‑M2/48‑1 (MQ=255)                                               ccagtaattcattatcggtaagttctggcagttcaaattcgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTGTGAAAACAGTGAATTATtaag                                                                                                                                                                                   <  2:171829‑M2/49‑2 (MQ=255)                                                cagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAtt                                                                                                                                                                                  >  1:69907‑M2/202‑251 (MQ=255)                                                    aattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCt                                                                                                                                                                               <  1:241667‑M2/53‑1 (MQ=255)                                                       tcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTtat                                                                                                                                                                            <  1:253605‑M2/56‑1 (MQ=255)                                                           tatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATaa                                                                                                                                                                        <  1:329633‑M2/60‑1 (MQ=255)                                                              cggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACAtt                                                                                                                                                                    >  1:141340‑M2/188‑251 (MQ=255)                                                                                                            gccataaatagcatcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatacgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCa                                                                                                                      <  2:250308‑M2/110‑1 (MQ=255)                                                                                                                     agcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACAt                                                                                                              <  2:180191‑M2/118‑1 (MQ=255)                                                                                                                     agcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACAt                                                                                                              <  2:303773‑M2/118‑1 (MQ=255)                                                                                                                       cagcaactttgggtttcatttttacctctgtaatcagtgtttatgttcggcggttaatttattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACAGGTGTTTTGTAAACAGTGAATTATTAATTTCTTATATCACATTGAGTTATAACTATAACAAAAAATTTCAATCCGAATATCGTGATGCATATTACATAAt                                                                                                           >  2:296473‑M2/131‑251 (MQ=255)                                                                                                                           aactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGat                                                                                                       >  1:62998‑M2/127‑251 (MQ=255)                                                                                                                             ctttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGatat                                                                                                     >  1:129980‑M2/125‑251 (MQ=255)                                                                                                                                tggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGAtattt                                                                                                   <  2:63759‑M2/129‑1 (MQ=255)                                                                                                                                     ttcatttttacctctgtaatcagtgtttatgttcgacggttaattgattaaaaaaagtggcgagttttggatcaccaatatagttattaatcaatatcagttgtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGc                                                                                              <  2:26738‑M2/134‑1 (MQ=255)                                                                                                                                      tcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCa                                                                                             <  2:402728‑M2/135‑1 (MQ=255)                                                                                                                                       catttttacctatttaatcagtgtttatgttctgcggataattgattatataaagtgtcgagttttggatagccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTTAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCTTATTTCATAATTGATATTTATTGCat                                                                                            <  2:267712‑M2/136‑1 (MQ=255)                                                                                                                                                                                        ataaagtgtcgagttttggatcgccaatatagttattactcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAAAAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAAc                                          >  2:323872‑M2/66‑251 (MQ=255)                                                                                                                                                                                                    gttttggatcgccaatatagttattactcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCa                                >  2:175772‑M2/54‑249 (MQ=255)                                                                                                                                                                                                         ggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATACCATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTCTTGCTTGTCCGCAAACGGACATATCAAGGtaat                         >  1:393218‑M2/49‑251 (MQ=255)                                                                                                                                                                                                         ggatcgcaaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGtaat                         >  2:373050‑M2/49‑251 (MQ=255)                                                                                                                                                                                                                caatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATaaaaa                    >  1:234114‑M2/42‑249 (MQ=255)                                                                                                                                                                                                                                caagatcagtggtttatccgtaacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTGATTGCATATAAATATTTGTGAGCTACTACACGCCTTATCTATTGCTTGTCCGCAAACAGACATATCAAGGTGATAAAAAATGTCGCCGAATCGACCt  <  2:334967‑M2/226‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                  GCGAGAATATAATAAGGGCCATTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT  >  NC_000913/3079152‑3079625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑