Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	389,867	T→C	intergenic (‑140/‑384)	hemB ← / → yaiT	porphobilinogen synthase/putative autotransporter YaiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	389,867	0	T	C	100.0%	261.6 / NA	66	intergenic (‑140/‑384)	hemB/yaiT	porphobilinogen synthase/putative autotransporter YaiT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/40);  total (26/40)

AAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATG  >  NC_000913/389621‑390110                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aaGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGCAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAActtt                                                                                                                                                                                                                                                  >  2:861657/1‑250 (MQ=255)      cGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATAtt                                                                                                                                                                                                                                             <  2:1045897/250‑1 (MQ=255)      cGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATAtt                                                                                                                                                                                                                                             <  1:356565/250‑1 (MQ=255)      cGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTc                                                                                                                                                                                                                                            <  1:684182/251‑1 (MQ=255)       ggCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTc                                                                                                                                                                                                                                            >  2:12203/1‑250 (MQ=255)       ggCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCa                                                                                                                                                                                                                                           <  1:564386/251‑1 (MQ=255)        gCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCAc                                                                                                                                                                                                                                          <  1:12203/251‑1 (MQ=255)         cAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACt                                                                                                                                                                                                                                         <  1:122782/251‑1 (MQ=255)         cAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACt                                                                                                                                                                                                                                         >  2:122782/1‑251 (MQ=255)          aaCACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTg                                                                                                                                                                                                                                        >  1:1047931/1‑251 (MQ=255)              ccAGGTCGATAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTGGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATATTTTTAATAGAAAACGCAATAACTTTATATTCACTGaaa                                                                                                                                                                                                                                     >  2:825710/1‑250 (MQ=255)               cAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGTATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATTCCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAat                                                                                                                                                                                                                                   <  1:825710/251‑1 (MQ=255)                  gTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAtt                                                                                                                                                                                                                                <  1:770336/251‑1 (MQ=255)                   tCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAttt                                                                                                                                                                                                                               >  2:548220/1‑251 (MQ=255)                    cGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAtttt                                                                                                                                                                                                                              <  1:641712/251‑1 (MQ=255)                     gTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAttttt                                                                                                                                                                                                                             >  2:1034525/1‑251 (MQ=255)                         aGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATc                                                                                                                                                                                                                         >  1:709012/1‑251 (MQ=255)                            cTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTAGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAttttt                                                                                                                                                                                                                             <  1:619435/244‑1 (MQ=255)                            cTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTAGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAttttt                                                                                                                                                                                                                             >  2:619435/1‑244 (MQ=255)                              aaGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtttat                                                                                                                                                                                                                    <  1:824850/251‑1 (MQ=255)                                     gtCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTc                                                                                                                                                                                                                                            <  1:1000898/220‑1 (MQ=255)                                     gtCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTc                                                                                                                                                                                                                                            >  2:1000898/1‑220 (MQ=255)                                     gtCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGc                                                                                                                                                                                                             <  1:793906/251‑1 (MQ=255)                                         ctTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTg                                                                                                                                                                                                           <  2:403538/249‑1 (MQ=255)                                           tCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGtt                                                                                                                                                                                                         <  2:347781/249‑1 (MQ=255)                                            cAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGt                                                                                                                                                                                                      <  2:538328/251‑1 (MQ=255)                                                cATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGttatt                                                                                                                                                                                                  <  2:1047931/251‑1 (MQ=255)                                                    gcgcgcAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAttt                                                                                                                                                                                              <  1:640099/251‑1 (MQ=255)                                                             gcagGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGtt                                                                                                                                                                                     <  1:441178/251‑1 (MQ=255)                                                              cagGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAata                                                                                                                                                                                                                                  >  2:907764/1‑205 (MQ=255)                                                              cagGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAata                                                                                                                                                                                                                                  <  1:907764/205‑1 (MQ=255)                                                              cagGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGttt                                                                                                                                                                                    <  2:545051/251‑1 (MQ=255)                                                                              gCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATaa                                                                                                                                                                    <  2:151184/251‑1 (MQ=255)                                                                                   gaGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                                                                                >  1:1040448/1‑250 (MQ=255)                                                                                   gaGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                                                                                >  1:1059821/1‑250 (MQ=255)                                                                                     gggggtgggtttaagctggTCATG‑TCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaata                                                                                                                                                            <  1:387287/233‑1 (MQ=255)                                                                                           tGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTa                                                                                                                                                        >  2:813977/1‑250 (MQ=255)                                                                                            ggATTAAGTCTGTCATGGTCTGCCTGATGGTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTaa                                                                                                                                                       <  1:440456/250‑1 (MQ=255)                                                                                              aTTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAAc                                                                                                                                                    >  1:480226/1‑251 (MQ=255)                                                                                              aTTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTGTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAAc                                                                                                                                                    <  1:977060/251‑1 (MQ=255)                                                                                                  aGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttg                                                                                                                                                >  2:709589/1‑251 (MQ=255)                                                                                                         cATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCat                                                                                                                                          >  1:656822/1‑250 (MQ=255)                                                                                                                 gCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAAGTGAATAATTAAAACTTTGTTGCATATTCATAg                                                                                                                                  <  1:861657/250‑1 (MQ=255)                                                                                                                        gTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATAttt                                                                                                                           <  2:1040448/250‑1 (MQ=255)                                                                                                                                     aGGGGCATAGTATACCTGAAGCAGGGCAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtt                                                                                                             >  1:17009/1‑251 (MQ=255)                                                                                                                                              gTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatt                                                                                                    <  1:31690/251‑1 (MQ=255)                                                                                                                                                  aacTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGCGGCCCGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttatt                                                                                                >  2:806153/3‑251 (MQ=255)                                                                                                                                                      gAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATg                                                                                             <  2:476696/250‑1 (MQ=255)                                                                                                                                                      gAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATg                                                                                             <  1:839765/250‑1 (MQ=255)                                                                                                                                                       aaGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATACCTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTtattatacg                                                                                           >  2:260050/1‑248 (MQ=255)                                                                                                                                                          cAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTAt                                                                                        <  2:87312/251‑1 (MQ=255)                                                                                                                                                                            cGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATc                                                                                                                        >  2:36833/1‑201 (MQ=255)                                                                                                                                                                                 gTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTc                                                                                                                                      <  1:674632/182‑1 (MQ=255)                                                                                                                                                                                 gTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTc                                                                                                                                      >  2:674632/1‑182 (MQ=255)                                                                                                                                                                                    gTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAAc                                                              >  2:730604/1‑251 (MQ=255)                                                                                                                                                                                         gATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGTTTTTTATTATGCGTATGCTTCAAAAACAAATTTATCTGCAACATGtt                                                         >  2:700361/1‑251 (MQ=255)                                                                                                                                                                                                    gaagaGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACtgtg                                              >  2:497677/1‑251 (MQ=255)                                                                                                                                                                                                         gaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCtttt                                         <  1:730604/251‑1 (MQ=255)                                                                                                                                                                                                           aTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTAt                                       <  1:548220/251‑1 (MQ=255)                                                                                                                                                                                                               aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTCTTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAAt                                   >  2:821530/1‑251 (MQ=255)                                                                                                                                                                                                               aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAAt                                   <  1:1034525/251‑1 (MQ=255)                                                                                                                                                                                                               aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAAt                                   <  2:480226/251‑1 (MQ=255)                                                                                                                                                                                                                           gACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTgg                                                                                                                     >  2:718698/1‑157 (MQ=255)                                                                                                                                                                                                                           gACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTgg                                                                                                                     <  1:718698/157‑1 (MQ=255)                                                                                                                                                                                                                           gACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGtt                       <  1:815484/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaTAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATaa            <  1:461941/251‑1 (MQ=255)                                                                                                                                                                                                                                                gCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTGGCATATTCATAGGATATTTATCTTGTTTGTGTTTGGGATATTTATTATGCTTATGCGTGATAAACAAAGTTATCTGGAACATGTGGAAATACTGTGGTGTTAGGAATTTGACGCTGGTTTTTCTCCATAAATTATATATg  <  2:356664/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGGGCGTTGGATTAAGTCTGTCATGGTCTGCCTGATGTTTGTGGAATCGAGGGGCATAGTATACCTGAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATG  >  NC_000913/389621‑390110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑