Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,518,356	A→G	intergenic (‑121/‑16)	fecI ← / → ykiE	RNA polymerase sigma factor FecI/protein YkiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,356	0	A	G	100.0%	277.7 / NA	72	intergenic (‑121/‑16)	fecI/ykiE	RNA polymerase sigma factor FecI/protein YkiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/28);  total (44/28)

GCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTATAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTCGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTG  >  NC_000913/4518111‑4518599                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    gCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATAAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAagg                                                                                                                                                                                                                                                >  2:597870/1‑251 (MQ=255)  cAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAaggt                                                                                                                                                                                                                                               <  2:23046/251‑1 (MQ=255)  cAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAaggt                                                                                                                                                                                                                                               <  2:455631/251‑1 (MQ=255)    aTGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCCAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGCCCTTTTTAACATGTTGTTTTTCTAAGTGTTGTAaggt                                                                                                                                                                                                                                               >  2:584910/1‑249 (MQ=255)         aTCTGCATCCAAAGCCGACTGGCGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTTATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGGAGGtat                                                                                                                                                                                                                                         >  2:555628/1‑250 (MQ=255)           cTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATaa                                                                                                                                                                                                                                       >  1:590559/1‑250 (MQ=255)                 cAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATggg                                                                                                                                                                                                                                 <  1:636310/250‑1 (MQ=255)                    aaGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATgg                                                                                                                                                                                                                             >  1:206488/1‑251 (MQ=255)                        aGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGcc                                                                                                                                                                                                                         <  1:292510/251‑1 (MQ=255)                         gACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCct                                                                                                                                                                                                                        >  1:444377/1‑251 (MQ=255)                                                                    gtgtgCCATAAAGCGACTCGACCGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTCATATGAGAATGGTTATCATTTCAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTc                                                                                                                                                                             >  2:512485/1‑251 (MQ=255)                                                                             aatgcgaCTCGATCGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAAACATGCGGAGGGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAAAATGTTGTTTTTCTACGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGTTCAGAATGACTCATGATGTGGTCTGCTActc                                                                                                                                                                     >  2:443751‑M1/4‑247 (MQ=255)                                                                             aaaGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTCCAATTGGAAATAAAATTGTTTCCAATAGACTTTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGATTAAAATGGGATGGGGCCTTTGCTTCTGGCTTGTGTCGGTCAGAATGATCCATGTTGTGTTCTGCTAccc                                                                                                                                                                     >  2:370429‑M1/1‑247 (MQ=255)                                                                               aGCGACTCGAACGTTAAGGAAGATGTGGTAGTGGCGCGGGCAGACATGCGGCGGGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCAGTACAATTGGAAATAAAATTGTTTCCACTAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGTCATGTGTCGGTCAGAATGACTCATGAAGTGGTCTGCTActca                                                                                                                                                                    >  2:548220‑M1/1‑245 (MQ=255)                                                                                      cGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaa                                                                                                                                                            <  2:319059‑M1/250‑13 (MQ=255)                                                                                             tAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaagggggaattgtgtc                                                                                                                                                     <  2:175927‑M1/250‑20 (MQ=255)                                                                                                ggAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcag                                                                                                                                                 <  2:296823‑M1/251‑24 (MQ=255)                                                                                                     cTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtatac                                                                                                                                            >  1:468181‑M1/1‑223 (MQ=255)                                                                                                              tGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgc                                                                                                                                         <  1:636429‑M1/245‑32 (MQ=255)                                                                                                              tGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgc                                                                                                                                         >  2:636429‑M1/1‑214 (MQ=255)                                                                                                               ggCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtatacctccagaaa                                                                                                                                   <  1:392240‑M1/250‑38 (MQ=255)                                                                                                               ggCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaac                                                                                                                                  <  2:325593‑M1/251‑39 (MQ=255)                                                                                                                 cgcgGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacgg                                                                                                                                >  1:81370‑M1/1‑211 (MQ=255)                                                                                                                 cgcgGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacgg                                                                                                                                <  2:590559‑M1/251‑41 (MQ=255)                                                                                                                     gTCAGACATGCGGAGAGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCACTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtatatcgccagaaacggggat                                                                                                                            >  2:152016‑M1/1‑207 (MQ=255)                                                                                                                       cAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggatt                                                                                                                           <  1:402817‑M1/250‑46 (MQ=255)                                                                                                                                   gTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacc                                                                                                              <  1:30605‑M1/251‑59 (MQ=255)                                                                                                                                     gCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacct                                                                                                             <  1:251654‑M1/250‑60 (MQ=255)                                                                                                                                            aaGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgtttcccg                                                                                                     <  1:394906‑M1/251‑68 (MQ=255)                                                                                                                                            aaGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccg                                                                                                     >  1:310178‑M1/1‑184 (MQ=255)                                                                                                                                               ttAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGCTGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggct                                                                                                  >  2:414820‑M1/1‑181 (MQ=255)                                                                                                                                                   ttATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGACATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacgg                                                                                              >  2:216788‑M1/1‑177 (MQ=255)                                                                                                                                                     aTCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCTAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggt                                                                                             <  2:294945‑M1/250‑76 (MQ=255)                                                                                                                                                     aTCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTATGCTActcaaggtggaattgtgtctcagtataccgccagaaacggggattaatactctttacctgttacccggccacggt                                                                                             >  2:63381‑M1/1‑175 (MQ=255)                                                                                                                                                       cACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacgtggattaatactctttacctgttacccgtctacggtagc                                                                                          <  2:12343‑M1/251‑79 (MQ=255)                                                                                                                                                         cGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtata                                                                                                                                             <  2:200251‑M1/198‑28 (MQ=255)                                                                                                                                                         cGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtata                                                                                                                                             >  1:200251‑M1/1‑171 (MQ=255)                                                                                                                                                            aGTCATATTAATATGAGAATGTTTATCATTACAATTGGAAATAAAATTGTTACCAATAGCCATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCAGGTGTCGGGCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcc                                                                                     <  2:204804‑M1/251‑84 (MQ=255)                                                                                                                                                               cATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccag                                                                                  <  1:597870‑M1/251‑87 (MQ=255)                                                                                                                                                                atatTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagt                                                                                 >  1:323326‑M1/1‑164 (MQ=255)                                                                                                                                                                atatTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagt                                                                                 >  1:220176‑M1/1‑164 (MQ=255)                                                                                                                                                                     aaTATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttga                                                                              >  2:38373‑M1/1‑159 (MQ=255)                                                                                                                                                                     aaTATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTATAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactatttacctgttacccggctacggtagctatcccagttgaag                                                                            <  1:5034‑M1/251‑93 (MQ=255)                                                                                                                                                                               tGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccac                                                                   >  1:446611‑M1/1‑149 (MQ=255)                                                                                                                                                                               tGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccac                                                                   >  1:228855‑M1/1‑149 (MQ=255)                                                                                                                                                                               tGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTAGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccac                                                                   >  2:395257‑M1/1‑149 (MQ=255)                                                                                                                                                                                    aTCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggaggaattgtgtcgcagtataccgccagaaacggggattaatactctttaccggttacccggctacggtagctatcccagttgaagatcacccacaaactg                                                             >  2:282828‑M1/1‑144 (MQ=255)                                                                                                                                                                                       aTTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgtt                                                           >  2:342751‑M1/1‑141 (MQ=255)                                                                                                                                                                                            aaTTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctg                                                                                                            <  1:249510‑M1/196‑61 (MQ=255)                                                                                                                                                                                            aaTTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctg                                                                                                            >  2:249510‑M1/1‑136 (MQ=255)                                                                                                                                                                                                ggAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgc                                                 >  1:552110‑M1/1‑132 (MQ=255)                                                                                                                                                                                                 gAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgca                                                <  2:420519‑M1/251‑121 (MQ=255)                                                                                                                                                                                                    aTAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatac                                             >  1:640980‑M1/1‑128 (MQ=255)                                                                                                                                                                                                         aTTGTTTCCAATAGCCATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGAATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGGGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccggaaacggggattaatactctttacctgttacccggctaaggtagctatcccagtttaagatcacccacaaactgttacccatgcgcatacggtcc                                        >  2:573791‑M1/1‑123 (MQ=255)                                                                                                                                                                                                          ttGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtcc                                        >  1:549099‑M1/1‑122 (MQ=255)                                                                                                                                                                                                            gTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatc                                     >  1:312389‑M1/1‑120 (MQ=255)                                                                                                                                                                                                              ttCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcac                                   >  1:207775‑M1/1‑118 (MQ=255)                                                                                                                                                                                                                ccAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgc                                 >  1:92048‑M1/1‑116 (MQ=255)                                                                                                                                                                                                                ccAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgc                                 >  1:267945‑M1/1‑116 (MQ=255)                                                                                                                                                                                                                   aTAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTGGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggcgacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgtt                              <  2:102884‑M1/251‑139 (MQ=255)                                                                                                                                                                                                                   aTAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgtt                              >  1:238408‑M1/1‑113 (MQ=255)                                                                                                                                                                                                                     aGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcg                            >  2:495731‑M1/1‑111 (MQ=255)                                                                                                                                                                                                                         aTTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgccca                        <  2:94585‑M1/251‑145 (MQ=255)                                                                                                                                                                                                                           ttttAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagc                      >  2:516412‑M1/1‑105 (MQ=255)                                                                                                                                                                                                                              tAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaa                    <  2:238408‑M1/250‑149 (MQ=255)                                                                                                                                                                                                                                aCATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatgctctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaactt                 <  1:555628‑M1/251‑152 (MQ=255)                                                                                                                                                                                                                                       gttTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcat           <  1:292918‑M1/250‑158 (MQ=255)                                                                                                                                                                                                                                         ttttCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctc        >  2:423541‑M1/1‑91 (MQ=255)                                                                                                                                                                                                                                          tttCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctct       >  1:114490‑M1/1‑90 (MQ=255)                                                                                                                                                                                                                                             cTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctcttcc    >  1:391853‑M1/1‑87 (MQ=255)                                                                                                                                                                                                                                             cTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctcttcc    >  2:635983‑M1/1‑87 (MQ=255)                                                                                                                                                                                                                                                aGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcatctcttccat  >  2:195489‑M1/1‑84 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTATAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTCGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTG  >  NC_000913/4518111‑4518599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑